ADVIA Centaur Immunoassay System BYZEESHAN YOUSUF
Technology
• Chemiluminescence is a chemical reaction that emits energy in the form of light. When used in combination with immunoassay technology, the light produced by the reaction indicates the amount of analyte in a sample. Direct chemiluminescent reactions directly measure the light energy without the use of added steps or amplifying molecules. The ADVIA Centaur XP assays use acridinium ester (AE) as the chemiluminescent label, because AE does not require the addition of a catalyst or substrate.
• Direct ChemiluminescenceIt is easy to automate direct chemiluminescence using AE
and provides many benefits, such as long reagent shelf life, fast reaction time, and assay sensitivity. The ADVIA Centaur XP assays use the dimethyl form of AE because its stability allows long reagent shelf life.
Assay Reaction Formats
The system uses a variety of formats to detect antigens as well as antibodies.
1
sandwich format
2
competitive format
3
antibody-capture format
ADVIA CENTAUR COMPONENTS
.
SYSTEM PROCESSING AREAS
Comprehensive Menu• 30 on board reagents maximize test capacity
Powerful Productivity• 240 tests per hour• High-resolution touch screen for easy interaction• Expanded ancillary reagent and fluid capacity• Universal sample rack design eliminates manual
tasks
• STAT port guarantees priority sampling at any time
Always Ready Replenish Supplies without Pausing 840 tips
USE OF SYSTEM
TECHNICAL SPECIFICATION
SAMPLE HANDLING
Sample Tubes•1 mL, 2 mL, 3 mL, 5 mL, 7 mL, 10 mL, micro container tubes
Automatic Dilutions •Varies by assay, up to 1:1000
Sample Handling •Serum, plasma, urine - assay dependent
Sample Volume •10 uL to 200 uL assay dependent
Sample Integrity Control •Pressure transducer technology with clot management, detection, and flagging.
REAGENT HANDLING
Reagent Cooling •30-position reagent tray cooled 4°C to 8°C
Reagent Integrity Control •Barcode reagent identification, automatic inventory tracking and flagging, calibration validity tracked and flagged, reagent on board stability tracked and flagged, reagent expired/ reagent low flagging
Reagent Mixing •ReadyPacks automatically rocked onboard
Quality Control
• Advanced QC package includes Levy-Jennings plots and Westgard rules; 65,000 control results can be stored• Results within ± 2SD accepted
TYPES OF ASSAYS
Allergy •Total IgE
Anemia•Ferritin•Folate•RBC Folate•Vitamin B12
Bone •Vitamin D
Tumor Markers/Oncology
•AFP•BR•CA 15-3•CA 19-9•CA 125II•CEA•Complexed PSA•Free PSA•PSA•Serum HER-2/neu
ToRCH and Special ID
•CMV IgG•CMV IgM•Rubella IgG•Rubella IgM•Syphilis•Toxo IgG•Toxo IgM
Cardiovascular
•BNP•CKMB II•D-Dimer•Myoglobin•Tnl-Ultra
Diabetes•C-Peptide (serum, urine)•Insulin
Hepatitis and
HIV
•Anti-HBe•Anti-HBs•Anti-HBs-2•eHIV 1/O/2†•HAV IgM•HAV Total•HBc Total•HBc IgM•HBe Ag•HBs Ag•HBs Ag Confirmatory•HBs AgII•HCV†•HIV Combo (CHIV)†
TDM
•Carbamazepine•Digitoxin•Digoxin•Gentamicin•Phenobarbital•Phenytoin•Theophylline•Tobramycin•Valproic Acid•Vancomycin
Liver Fibrosis
•HA (ELF Marker)•TIMP (ELF Marker)•PIIINP (ELF Marker)
Metabolic •Cortisol (serum, urine)•Homocysteine
Reproductive Endocrinolog
y
•AFP•DHEAS•Estradiol-6•Estradiol-6 III•FSH•LH•Progesterone•Prolactin•SHBG•Testosterone•Total hCG
Immunosuppresants
•Cyclosporine•Tacrolimus*
Sepsis •Procalcitonin
Thyroid Function
•Anti-TG Ab•Anti-TPO Ab•Free T3•Free T4•Intact PTH•TSH•Third Generation TSH•T Uptake•Total T3•Total T4•TSH 3-UL
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