113,612 high-quality 3’-end ESTs

• CAP3 assembly

32,980 transcripts

Filter for FL candidate criteria• 2,771 missing 5’ SSPA• 1,312 short 5’- and/or 3’-end ESTs (Q20 < 400 nt)• 920 missing polyA tail• 357 5’-end ESTs > 100 nt shorter than public match

• Select PUTs with FLcDNA library clones

10,710 candidate FL transcripts

ROUND 1147,146 high-quality

3’-end ESTs

ROUND 2

46,745 transcripts

9,759 candidate FL transcripts

Filter for FL candidate criteria• 3,673 missing 5’ SSPA• 1,514 short 5’- and/or 3’-end ESTs (Q20 < 400 nt)• 1,119 missing polyA tail• 450 5’-end ESTs > 100 nt shorter than public match

• Select PUTs with FLcDNA library clones• Discard clones selected in round1

• CAP3 assembly

5,348 candidates selected

2,779 candidates selected

5,298 clones finished(65.1%)

• Rearray into 384-well plates• 2x 3’-end and 2x 5’-end per clone

1,166 clones finished(14.3%)

• Rearray into 96-well plates• Gap-closing by primer walking

1,396 clones incomplete(17.1%)

267 clones aborted(3.2%)

• Manual and automated inspection for finished quality

• Manual and automated inspection for finished quality