Yeasts - WIV-ISPBudding yeasts and pseudomycelium in urine sediment (Unstained). Candida tropicalis...
Transcript of Yeasts - WIV-ISPBudding yeasts and pseudomycelium in urine sediment (Unstained). Candida tropicalis...
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Yeastsin clinical specimens and in culture
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Candida albicans Gram-positive staining yeastcells and polymorpho-
nuclear leukocytes in sputum (Gram stain).
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Candida albicans C. albicans in a vaginal smear. The budding pseudomycelium is much broader than the Gram-
positive lactobacilli (Gram stain).
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Candida albicans C. albicans in a vaginal smear. The pseudomycelium
is much broader and longer than the Gram-positive lactobacilli (Gram stain).
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Candida albicans Abundant Gram-positive pseudomycelium and
epithelial cells in saliva (Gram stain).
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Candida albicans Pseudomycelium, blastospores, and Gram-positive
rods in a case of thrush (Gram stain).
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Candida albicans Pseudomycelium, blastospores, and Gram-positive rods in oesophagitis from an HIV-patient (Gram stain).
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Candida albicansPseudomycelium in faeces (Gram stain).
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Candida albicans In urinesediment: many epithelial cells, lactobacilli
and pseudomycelium (Gram stain).
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Candida albicans Blastospores in faeces. They are smaller ( 3 to 4 μ) than the
smallest cysts and have no typical structure. They also stain dark violet with Gram’s method (Lugol stain).
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Candida albicans Mycelium, round to slightly oval blastospores, and two
thick-walled chlamydospores in a slide culture on corn-meal agar (Cotton blue stain).
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Candida albicans Mycelium and many thick-walled chlamydospores in a
slide culture on corn-meal agar (Cotton blue stain).
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Candida albicans Mycotula aspect: mycelium and round to slightly oval
blastospores in clusters around the mycelium in a slide culture on corn-meal agar (Cotton blue stain).
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Candida albicans This species and Candida stellatoidea form «germ tubes»within 3 to 4 hours of incubation in serum. This phenomenon is
used for rapid identification (Unstained).
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Candida albicans This species and Candida stellatoidea form «germ tubes»within 3 to 4 hours of incubation in serum. This phenomenon is
used for rapid identification (Unstained).
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Candida albicans This species and Candida stellatoidea form «germ tubes»within 3 to 4 hours of incubation in serum. This phenomenon is
used for rapid identification (Unstained).
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Candida albicans A Gram stain of Candida albicans in culture shows
yeasts and mycelium (Gram stain).
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Candida glabrata Absence of mycelium, but presence of round to slightly oval small blastospores in a slide culture on
corn-meal agar (Cotton blue stain).
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Candida glabrata Absence of mycelium, but presence of round to
slightly oval small blastospores in a culture on Sabouraud agar (Gram stain).
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Candida krusei Mycocandida aspect: mycelium and typical elongated
blastospores branching from the mycelium in a slide culture on corn-meal agar (Cotton blue stain).
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Candida kruseiElongated blastospores in broth (Unstained).
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Candida kruseiA thin powdery pellicle that creeps up against the glass
wall of a tube of Trypticase Soy Broth (TSB).
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Candida parapsilosis Mycocandida aspect: delicate mycelium and round to
slightly oval blastospores in a slide culture on corn-meal agar (Cotton blue stain).
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Candida pseudotropicalis Mycocandida aspect: delicate mycelium and round to
slightly elongated blastospores in a slide culture on corn-meal agar (Cotton blue stain).
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Candida sake Absence of mycelium, but presence of round to slightly oval small blastospores in a slide culture on
corn-meal agar (Cotton blue stain).
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Candida tropicalis Budding yeasts and pseudomycelium in urine sediment
(Unstained).
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Candida tropicalis Delicate mycelium and round to slightly oval blastospores in a slide culture on corn-meal agar
(Cotton blue stain).
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Candida zeylanoides Mycocandida aspect: delicate mycelium and round to
slightly oval blastospores in a slide culture on corn-meal agar (Cotton blue stain).
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Cryptococcus neoformans Round large yeast cell with bud, surrounded by a translucent capsule in cerebrospinal fluid (India ink
stain).
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Cryptococcus neoformans Round large yeast cells, surrounded by a capsule in
pus (Gram stain).
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Cryptococcus neoformans Round large budding yeast cells in culture (Gram
stain).
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Cryptococcus neoformans Absence of mycelium and round blastospores of varying diameter in a slide culture on corn-meal agar
(Cotton blue stain).
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Geotrichum candidum Large arthrospores and round blastospores of G.
candidum in faeces can sometimes be mistaken on direct examination for cysts of protozoa (Lugol stain).
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Geotrichum candidum Arthrospores and large blastospores of G. candidum in
faeces can be differentiated from cysts of protozoa by Gram stain (Gram stain).
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Geotrichum candidum Large arthrospores in a slide culture on corn-meal agar
(Cotton blue stain).
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Geotrichum candidum Large arthrospores in a culture on Sabouraud’s agar
(Unstained).
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Malassezia furfur Round spores and small fragments of mycelium in skin
scrapings from a lesion of tinea versicolor (Stained with Parker ink in potassium hydroxide).
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Malassezia furfur Round spores and small fragments of mycelium in skin
scrapings from a lesion of tinea versicolor (Stained with Parker ink in potassium hydroxide).
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Pityrorosporum ovale (syn.Malassezia furfur)
Small yeast cells in a peripheral blood smear from a patient receiving intravenous lipid-enriched alimentation (May-Grünwald-Giemsa stain).
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Rhodotorula sp. Round and oval blastospores in culture (Unstained).
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Saccharomyces cerevisiae The ascospores are stained green by malachite green,
and the blastospores red with safranin (Wirtz stain).
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Trichosporon beigelii (syn. cutaneum)
Mycelium, round to ovoid blastospores and rectangular arthrospores in a slide culture on corn meal
agar (Cotton blue stain).
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Trichosporon beigelii (syn. cutaneum)
Mycelium, round to ovoid blastospores and rectangular arthrospores in a slide culture on corn meal
agar (Unstained).
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Trichosporon capitatum Mycelium, elongated arthrospores, and blastospores in
a slide culture on corn meal agar (Unstained).