XBridge Columns

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XBridge™ HPLC Columns


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First introduced in 1999 in XTerra® columns, Waters patented organic/inorganicHybrid Particle Technology [HPT] surmounted significant limitations of silica-basedreversed-phase packing materials, particularly their hydrolytic instability at high pH.In 2005, second-generation HPT, branded as BEH Technology™, embodied in new

ACQUITY UPLC™ BEH Columns and in Waters XBridge™ HPLC columns, marks anew milestone in chromatography.

With an order-of-magnitude improvement in high pH stability and a higher level ofchromatographic performance, BEH Technology™ columns define the new benchmark for

LC method development.

A New Milestone in Chromatography


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3

BEH Technology™, the second generation patented hybrid particle*, is a product of Waters commitment tocontinued investment in particle Research and Development, providing reliable solutions to complex method

development problems. Based upon crucial customer feedback, three goals for this second-generationhybrid particle were considered paramount.

• Maximize efficiency

• Further improve high-pH stability

• Improve column reliability

After four years of research, we are proud to introduce BEH Technology™ hybrid particles.

BEH Technology™ synthesis creates particles that ensure extreme column performance and long column lifetime under harsh operatingconditions. These particles are prepared from two high purity monomers: tetraethoxysilane [TEOS] and bis(triethoxysilyl)ethane [BTEE,which incorporates a pre-formed ethylene bridge].

Introduction

BEH Technology ™

. .

Tetraethoxysilane(TEOS)

Polyethoxysilane(BPEOS)

Bis(triethoxysilyl)ethane(BTEE)

+4

Si

EtO

EtO OEtEtO

Si

EtO

EtO

EtO

Si

OEt

OEt

OEt

Eth ylene Bridgesin Silica Matrix

Si

Et O

O

CH 2 CH2

CH2

CH2

Si O Si

EtO

OEt

Si

O

O

OEt

Si

O

SiOO

Et

O

OO

Et

Et Et n

Anal. Chem. 2003, 75, 6781-6788

* Patent # 6,686,035 B2

BEH Technology ™ Particle Synthesis

One of the most important parameters in the design of the new hybrid particle was to significantly improvethe chromatographic equivalence to state-of-the-art silica based materials. The origins of band spreading,

which decreases separation efficiency, are described by the van Deemter equation:

h = a + b /υ + c υ

Data for both silica and BEH Technology™ packings, fitted to the van Deemter equation demonstrate effectiveequivalence in efficiency. The c term for XBridge™ C18 is virtually identical to that for the two state-of-the-art C 18silica columns, indicating that all these columns are comparable in mass transfer characteristics.

Maximizing Column Efficiency

van Deemter Curve Comparison

2

4

6

8

10

12

0 20 40 60 80

u [uid

p/D

m]

Reduced van Deemter Plot Terms

a b c r2

XBridge™ C18

5µm 1.04 8.7 0.044 0.998

Symmetr y® C18

5µm 0.76 10.6 0.045 0.999

SunFire™ C18

5µm 0.95 11.6 0.040 0.997

h using N1/2

Ht Anal yte: Decanophenone

Mobile Phase: 70/30 ACN/waterTemperature: 30 °C

The reduced plate height, h, is a function of the reduced linear velocity, υ , [both normalized for particle size] and a, b , andc summarize the contributions of eddy diffusion, longitudinal diffusion, and the sum of stationary- and mobile-phase masstransfer terms, respectively.


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Probe: Methylparaben

50

60

70

80

90

100

0 50

100

Hours in1% TFA in H2O pH 1.0 at 80 °C

%

I n i t i a l R e t e n t i o n

XBridge™ C18

Zorbax® SB-C18

XBridge™ Phenyl

Zorbax® SB Phenyl

Luna® C18(2)

ACT Ace® C18

Gemini™ C18

Capcell PAK MG II

4

The predominant cause of shortened column lifetime in low pH mobile phases relates to the acid hydrolysis of thebonded phase. This hydrolysis can lead to significant changes in analyte retention time, making method suitability

requirements difficult to achieve.XBridge™ packings incorporate the use of well-characterized, state-of-the-art, proprietary procedures for bonding andendcapping. Bonded phases prepared using these procedures are much more stable and reproducible at low pHthan conventionally prepared materials and resist the typical ligand hydrolysis failure. In an accelerated lowpH stability test, XBridge™ C18 columns show very little retention loss and exhibit a column lifetime equivalent to thatof a sterically hindered C18-silica-bonded phase.

Improved Low pH Performance

pH Stability

XBridge™ columns have been specifically designed to be the most pH stable high-performance chromatographicphases commercially available. Unlike approaches which claim high pH resistance due to special surface

modifications, XBridge™

columns have stability built in as part of the particle synthetic process.Under accelerated pH 10 stability test conditions, a direct comparison to some of the most popular chromatographicphases, claimed to have extended high pH stability, clearly shows the XBridge™ C18 column lifetime exceeding that ofthe best silica column by over 1000% with very little degradation in chromatographic performance.

Over 1000% Longer High pH Endurance

Accelerated Low pH Stability Tests of Competitive Columns

USP Tailing Factors at pH 3 and pH 7

The combination ofexcellent particle andligand stability as well ashigh chromatographicefficiencies makesXBridge™ columns anideal choice for low andintermediate pH methods.

Analyte: Acenaphthene

030

50

70

90

110

50 100 150 200 hours

Hours in 50 mM TE A , pH 10, 50° C

%

I n i t i a l N

5 s

XBridge™ C18

XTerra® MS C18

Gemini™ C18

Luna® C18(2)

YMC™ Pro C18

Zorbax® Extend C18

Accelerated High pH Stability Test of Competitive Columns

0 4 8 12 16 20 24

Initial

100 h

200 h

300 h

N=13600

N=13500

N=14100

N=11700

12

34

5

Chromatograms, run at regular intervals during the high-pH lifetime study , verify that 86% of the original XBridge™ column efficiencyremains after 300 hours at pH 10 and elevated temperature, with little change in peak shape or retention time.

XBridge™ Family

Desipramine

Amitriptyline

pH 7

Nortriptyline

Amitriptyline

pH 3

USP Tailing Factors

1

2

3

C8 Phenyl RP18 C18 Zorbax®

SB-C18

Compounds1. Uracil2. Propranolol3. Naphthalene4. Acenaphthene5. Amitriptyline

Minutes


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3OH-

Si

Si(OH)4

O

O O Si

O

O Si

O

O Si

O

O Si

O

O Si

O

OHOH

OSi

O

O O

O O Si

O

O O O O

O Si

O

O O O O O O O

OH OH OH

SiO O Si O Si O Si O Si

O Si

OSiO O Si O Si

Surface

Core

SiO O

Si

O

O

Si

O

O Si

O

O Si O Si

O

OHOH

OSi

O

CH2 O

O O Si

O

OH OH O

O Si

CH2

CH2

O O O O O O O

OH OH CH2 CH2

CH2

CH2

Si

O O

O

SiO

SiO

SiO

SiO

SiO

Si

Si

O

O

O

Si

Si

O

CH2

OH

SiO O

O

O

O

O Si

O Si

Core

5

Chromatographic Lifetime Comparisons Structural Particle Integrity Resists High pH Attack

270 Hours

13 Hours

1050 15 20 1050 15 20

300 Hours 30 Hours

N = 2218N = 11700

100 20 3040 20 2416128

The choice of mobile phase buffer and concentration, particularly at elevated pH, has a profound impact not onlyon chromatographic peak shape but also on column lifetime. In order to be successful, the method development

chemist requires the flexibility to choose the appropriate buffer for selectivity and detection technique.The XBridge™ family of columns exhibit maximum stability in the widest range of volatile and non-volatile buffer types.

Every XBridge™ column product is shipped witha technical information CD. Included in thisinformation is a chart showing the full range ofcompatible buffer types, concentrations, andeffective pHs.

Column Lifetime and Buffer Choice

The uniform distribution, and effective cross-linking of the ethylene-bridged co-monomer throughout the backbone, deliverschromatographic particles which exhibit unprecedented mechanical strength and hydrolytic stability.

Unbonded silica particles are extremely unstable in alkaline mobile phases. The failure mechanism generallyaccepted as predominant is nucleophilic attack by hydroxide ions on its structural siloxane bonds.

XBridge™ particles exhibit excellent resistance to high pH solutions, even prior to bonding, due to the presence ofstructural ethylene bridges which do not easily hydrolyze. Up to six siloxane bonds would have to be broken to freeone ethylene-bridge unit. This fact, coupled with the excellent mechanical strength of the particle, explains theexceptional lifetime of XBridge™ columns in high pH mobile phases.

Maximum Particle Stability

Silica Particle

XBridge™ Particle

XBridge™ C18 Gemini™ C18

XBridge™ C18 Gemini™ C18

50 mM TEA, 50 °C, pH 10

30 mM KH2PO4, 30 °C, pH 12.3

Minutes Minutes

Minutes Minutes


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The development route to a final, robust LC method may require investigation of many selectivity tools, such as bond-ed ligands, solvent type, modifiers, temperature and most importantly pH. This route is complicated further by the fact

that modern column chemistries usually are designed to excel in only a limited combination of these conditions,making the selection of the most suitable column extremely difficult.

The XBridge™ family of columns is designed to eliminate this compromise and deliver the flexibility to work under anymobile phase, temperature and pH conditions, thus speeding up the process to an optimum and rugged finalmethod (recommended operating limits are shown on page 9).

The Universal Family of Columns

Method Development Flexibility

The XBridge™ family of columns introduces a new level of chromatographic performance and confidence to themethod development chemist. Robust, rugged methods can now be achieved across the entire pH range of 1-12,

simplifying the validation and method transfer process.Utilizing a simple method development protocol, consisting of XBridge™ columns, two mobile phase pHs, and twoorganic solvents, even the most challenging of sample mixtures can be quickly resolved.

Efficient Method Development

111

1

1 .

111

1, ,

1

1

1

,

11

1

C18

Phenyl

Shield RP18

C18

Phenyl

Shield RP18

XBridge™ Low p H in Acetonitrile XBridge™ High pH in Acetonitrile

11

1

1

,11

11

1

1

1,1

11

1

1

1

1

2

1

3

4,5

119

8

6 7

12

10

2

1

1110

5

4

8

6

7

9

123

2

1

3

119,10

5

4

8

6

7

12

11

62

4

1

3

13912

5

7

10

8

11

6

2

4

1

3

139

12

5

7

10

8

11,12

62

4

1

3

139

5 810

7

. . . . . .

0.0 4.00 8.00 12.00 16.00 20.00

. . . . . .

0.0 4.00 8.00 12.00 16.00 20.00

1110

2

3

1

9

5

4

6

7

8

16.68

1110

1,2

3

8,9

5

6

12

4 7

2

3

1

10

57,8

6

11

12

4

9

XBridge™ Low pH in Methanol

C18

Shield RP18

Phenyl

C18

Shield RP18

Phenyl

XBridge™ High pH in Methanol

11

62

1

3 12

8,1310

79

5

11

62

1

3 12

139,10

7 8

5

11

62

1

3 12

13

9

10

5

7

1

,

11

1

1

1

111

1

1

11,1

1

11

1

1

1

1

11

1

1

1

1

11,1

1

1

1

0.0 4.00 8.00 12.00 16.00 20.00

. . . . . .

0.0 4.00 8.00 12.00 16.00 20.00

. . . . . .

N

0

1

2

3

4

5

6

7

8

9

10

11

12

13

14

0 1 2 3 4 5 6 7 8 9 10 11 12

0 1 2 3

pH

pH 10

pH 5

pH 2

B

B

A,B

A

A

N

N

0 1 2 3

0 1 2 3

C a p a c i t y

F a c t o r ( k )

Minutes

Neutral (N)

Acid (A)

Base (B)

For the analysis of ionizable compounds, pH provides the most powerful tool in optimizing selectivity. Dramatic changes in theretention time and peak shape of acidic and basic molecules will occur purely as a function of the mobile phase pH.

The Effect of pH on Retention for Acidic, Basic, and Neutral Analytes


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With the introduction, and subsequent widespread adoption, of high purity reversed phase silica columns in the early1990’s, the method development chemist has been unable to freely utilize phosphate buffers (pH7 and above) due

to their extremely aggressive nature. The combination of phosphate, intermediate pH and relatively low temperatures(40 °C) is well recognized to lead to short column lifetimes for most modern phases.

Phosphate buffers however do have quite desirable qualities for a non-MS based assay

• Excellent UV transparency

• Unique peak shape and selectivity characteristics

• Good buffering capacity at multiple pK a values

XBridge™ and Phosphate Buffer

The increasing productivity demands on the chromatography laboratory present an additional tough

challenge to the method developer. Once an initial method has been established, optimization of the totalcycle time becomes the key parameter.

The availability of multiple particle sizes and dimensions allows the optimization of the total cycle time withoutsacrificing resolution.

Particle Sizes and Dimensionsfor Method Optimization

pH 2

pH 7

pH12

1

26

3 54

23

1

4

6 5

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00

3 2

465

1

Phosphate Buffer pH Selectivity

Maintaining Resolution with Constant Length/Particle Size Ratio

. . . . . .

0.00 0.40 0.80 1.20 1.60 2.00

0.00

5 µm – 150 mmInjection = 5.0 µLRs (2,3) = 2.28

Flow Rate: 0.2 mL/min

3.5 µm – 100 mmInjection = 3.3 µLRs (2,3) = 2.32Flow Rate: 0.3 mL/min

2.5µm – 75 mmInjection = 2.5 µLRs (2,3) = 2.34Flow Rate: 0.5 mL/min

.I j i .

l : . i

2.00 4.00 6.00 8.00 10.00

0.00 3.002.001.00 4.00

1 2 3

4

0.00 0.20 0.40 0.60 0.80 1.00 1.10

. . . . .

.

I j i .

l : . i

.I j i .

l : . i

.I j i .

l : . i

1.7 µm – 50 mmInjection = 1.7 µLRs (2,3) = 2.29Flow Rate: 0.6 mL/min

. . . .

. ...

ACQUITY UPLC™ systems and BEH chemistries are specifically designed to enable the quickest method development procedures,fastest cycle times while delivering maximum resolution.

Reference ACQUITY UPLC™ brochure, Literature number 720001140EN

XBridge™ columns are unique in their ability to withstand these aggressive conditions (even at pH 12) allowing complete flexibility forthe method development chemist, while maintaining the highest efficiency values associated with silica columns.

Mo bi le Ph as e A: 0 .1 % FA in H2OMo bi le Ph as e B : 0 .1 % FA in AC NIsocratic: 95% A: 5% B

Compounds1. 1-methylxanthene2. 1,3-dimethyluric acid3. theobromine4. 1,7-dimethylxanthene

Column: XBridge™ C18, 3.5 µm4.6 x 100 mmMobile Phase: A1: 30 mM potassium phosphate buf fer (pH 2)

A2: 30 mM potassium phosphate buffer (pH 7)A3: 30 mM potassium phosphate buffer (pH 12)

Mob il e P hase B : A ce to ni tr il eFlow Rate: 1.4 mL/minGradient: Time Profile

(min) %A %B0.0 90 107.0 20 808.0 20 80

I nj ec tio n Vo lum e: 20 µLSample Concentration: 50 µg/mL each in

80:20 Water:MethanolTemperature: 30°CDetection: UV @ 210 nm (pH 2,7);

220 nm (pH 12)Instrument: Waters Alliance® 2695

with 2996 PDA

Compounds1. Doxylamine2. Benzamide3. Hydroxyisophthalic Acid4. Doxepine5. Flavone6. Fenoprofen

HPLC

UPLC™

Minutes

MinutesMinutes


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Poor lifetimes and the lack of consistent performance of preparative columns have been a significant element of

concern to the purification chemist. Cost per purification needs to be reduced and the loss of samples due to earlycolumn failure must be minimized. After many years of research into column packing and design, Watersintroduced the patent pending Optimum Bed Density (OBD™) preparative column designs, effectively answeringthese concerns. This format is now recognized in the industry as delivering the most stable efficient and reproduciblepreparative columns.

The combination of the rugged XBridge™ packings and the OBD™ design takes preparative column performance toa new level, ensuring direct scalability, maximum efficiencies and the longest column lifetimes.

OBD™ Preparative Columns

Direct Scalability Maximum Efficiency/30% Lower Backpressure

0.00 2.00 4.00 6.00 8 .00 10.00 12.00

12

14.00 16.00 18.00 20.00

The OBD™ preparative column design delivers the equivalent packed bed density to that of the analyticalcolumn and therefore ensures direct scalability.

Preparative packing materials are routinely offered with a much wider particle size distribution than the equivalentanalytical particle. While this approach does reduce the cost of material manufacturing, the chromatographic

performance is almost certainly compromised.XBridge™ Prep packing materials are fully optimized to deliver maximum efficiency and the highest loadability at thelowest possible back-pressures. These attributes allow the purification chemist the flexibility to choose the bestcombination of particle size and column dimension to easily purify even the most complex of samples.

A Particle Designed for PurificationReaching a New Level inPreparative Column Durability

0.00 2 .00 4.00 6.00

1 2

3

4

5

8.00 10.00 12.00 14.00 16.00 18.00 20.00

XBridge™ Prep Columns deliver the same high loading capacity and reliability expected of ourXTerra® Prep products, with a significantly reduced column backpressure.

XBridge™ C18 4.6 x 100 mm, 5 µmInjection Volume: 30 µLTotal Load: 6 mg

Mobile Phase A: 10 mM ammonium ace tate , pH 10Mobile Phase B: acetonitri le/100 mM ammonium acetate (90/10)F low Ra te : 1 .0 6 mi n/m L ( an a); 18 min /mL (p re p)G ra die nt : 1 0- mi n g ra die nt fr om 5 %B t o 9 5%BDetection: UV @ 270 nm

Compounds1. Econazole (100 mg/mL)2. Miconazole (100 mg/mL)

XBridge Prep C18 19 x 100 mm, 5 µmInjection Volume: 510 µLTotal Load: 102 mg

XTerra® Prep MS C18, 19 x 50 mm, 5 µmInjection Volume: 660 µLTotal Load: 198 mgMaximumBackpressure: 1000 psi

XBridge™ Prep C18, 19 x50 mm, 5 µm

Injection Volume: 660 µLTotal Load: 198 mgMaximumBackpressure: 700 psi

Mobile Phase A: 0.1% DEA in waterMobile Phase B: 0.1% DEA in acetonitrileSample Concentration: 300 mg/mL in DMSOInstrument:: AutoPurification® SystemDetection: UV @ 260 nm

Compounds1. Labetolol (50 mg/mL)2. Quinine (50 mg/mL)3. Diltiazem (50 mg/mL)4. Verapamil (100 mg/mL)5. Amitriptyline (50 mg/mL)

MinutesMinutes

Flow Rate:23.8 mL/minGradien t: T ime Pro fi le

(min) %A %B0.0 95 51.79 95 56.79 5 957.79 5 95

Injection Volume: 510 µL

Flow Rate:23.8 mL/minGradien t: T ime Pro fi le

(min) %A %B0.0 95 51.79 95 56.79 5 957.79 5 95

Injection Volume: 510 µL


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XBridge™ Column Characteristics

O Si

O

O

i l

O Si

O

O

i l

O Si

CH

PolarGroup

3

CH3

O Si

O

O

i l

Ligand Type Trifunctional C18 Trifunctional C8Monofunctional

Embedded Polar GroupTrifunctionalC6 Phenyl

AvailableParticleSizes (µm)

2.5, 3.5, 5, 10** 2.5, 3.5, 5, 10** 2.5, 3.5, 5, 10** 2.5, 3.5, 5, 10**

LigandDensity*

3.1 µmol/m2 3.2 µmol/m2 3.3 µmol/m2 3.0 µmol/m2

Carbon Load* 18% 13% 17% 15%

Endcap Style Proprietary Proprietary TMS Proprietary

pH Range 1-12 1-12 2-11 1-12

Low pHTemp. Limits

80 °C 60 °C 30 °C 80 °C

High pHTemp. Limits

45 °C 45 °C 45 °C 45 °C

PoreDiameter*

135Å 135Å 135Å 135Å

Pore Volume* 0.7 mL/g 0.7 mL/g 0.7 mL/g 0.7 mL/g

Surface Area* 185 m2/g 185 m2/g 185 m2/g 185 m2/g

* Expected or Approximate Values** 10 µm available late 2005

B on d e d

P h a s e

In order to explore the limits of high-pH stability, the entire family of XBridge™ columns was subjected at 50 °C to a0.02 N NaOH mobile phase at pH 12.3. The lifetimes of all XBridge™ columns ranged from 28 to 45 hours under

conditions that cause silica-based columns to fail within 1 to 2 hours.For the first time, a family of columns stable at the extremes of pH conditions is now available, dramaticallysimplifying the entire method development process.

Exploring the Extremes

0 10 20 30 40 50

XBridge™ C8

XBridge™ C18

XBridge™ Phenyl

XBridge™ Shield RP18

XTerra® MS C18

Symmetry® C18

Hours in 0.02N NaOHpH 12.3 at 50 °C to 50% Efficiency Loss

Exploring the Limits of XBridge™ Column Lifetime at pH 12.3

XBridge™ Phenyl, C8, and C18 columns have nearly equivalent high-pH stability, in marked contrast to silica-based columns where theshorter-chain primary functional groups [phenyl, C8] typically fail much sooner than their C18 counterparts.

Challenge mobile phase: 0.02N NaOH pH 12.3 at 50 °CTest mobile phase: 50/50 acetonitrile/water at 50 °CTest probe: Decanophenone

B E H

P

ar t i c l e


10/1210

XBridge™ Ordering Information

Dimensions Type Particle Size C18 C8 Shield RP18 Phenyl

1.0 x 50 mm Column 2.5 mm 186003118 186003164 186003136 1860033062.1 x 10 mm Guard 2.5 µm 1860030563 1860030743 1860030653 1860033593

2.1 x 20 IS

™

Column 2.5 µm 186003201 186003167 186003139 1860033072.1 x 30 mm Column 2.5 µm 186003084 186003099 186003091 1860033082.1 x 50 mm Column 2.5 µm 186003085 186003101 186003092 1860033093.0 x 20 mm IS ™ Column 2.5 µm 186003087 186003168 186003140 1860033103.0 x 20 mm Guard 2.5 µm 1860030574 1860030754 1860030664 18600336043.0 x 30 mm Column 2.5 µm 186003121 186003169 186003141 1860033113.0 x 50 mm Column 2.5 µm 186003122 186003170 186003142 1860033124.6 x 20 mm IS ™ Column 2.5 µm 186003088 186003172 186003144 1860033134.6 x 20 mm Guard 2.5 µm 1860030584 1860030764 1860030674 1860033614

4.6 x 30 mm Column 2.5 µm 186003089 186003173 186003145 1860033144.6 x 50 mm Column 2.5 µm 186003090 186003174 186003096 1860033154.6 x 75 mm Column 2.5 µm 186003124 186003175 186003146 186003316

1.0 x 50 mm Column 3.5 µm 186003126 186003177 186003148 186003317

1.0 x 100 mm Column 3.5 µm 186003127 186003178 186003149 1860033181.0 x 150 mm Column 3.5 µm 186003128 186003179 186003150 1860033192.1 x 10 mm Guard 3.5 µm 1860030593 1860030773 1860030683 18600336232.1 x 20 mm IS ™ Column 3.5 µm 186003019 186003180 186003151 1860033202.1 x 30 mm Column 3.5 µm 186003020 186003046 186003035 1860033212.1 x 50 mm Column 3.5 µm 186003021 186003047 186003036 1860033222.1 x 100 mm Column 3.5 µm 186003022 186003048 186003037 1860033232.1 x 150 mm Column 3.5 µm 186003023 186003049 186003038 1860033243.0 x 20 mm IS ™ Column 3.5 µm 186003024 186003181 186003152 1860033253.0 x 20 mm Guard 3.5 µm 1860030604 1860030784 1860030694 18600336343.0 x 30 mm Column 3.5 µm 186003025 186003182 186003153 1860033263.0 x 50 mm Column 3.5 µm 186003026 186003050 186003039 1860033273.0 x 100 mm Column 3.5 µm 186003027 186003051 186003040 1860033283.0 x 150 mm Column 3.5 µm 186003028 186003052 186003041 186003329

4.6 x 20 mm IS ™

Column 3.5 µm 186003029 186003183 186003154 1860033304.6 x 20 mm Guard 3.5 µm 1860030614 1860030794 1860030704 1860033644

4.6 x 30 mm Column 3.5 µm 186003030 186003184 186003155 1860033314.6 x 50 mm Column 3.5 µm 186003031 186003053 186003042 1860033324.6 x 75 mm Column 3.5 µm 186003032 186003185 186003043 1860033334.6 x 100 mm Column 3.5 µm 186003033 186003054 186003044 1860033344.6 x 150 mm Column 3.5 µm 186003034 186003055 186003045 186003335

2.1 x 10 mm Guard 5 µm 1860030623 1860030803 1860030713 18600336632.1 x 20 mm IS ™ Column 5 µm 186003107 186003186 186003156 1860033362.1 x 30 mm Column 5 µm 186003129 186003187 186003157 1860033372.1 x 50 mm Column 5 µm 186003108 186003011 186002999 1860033382.1 x 100 mm Column 5 µm 186003109 186003012 186003002 1860033392.1 x 150 mm Column 5 µm 186003110 186003013 186003003 186003340

XBridge™ Analytical Columns


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3.0 x 20 mm IS ™ Column 5 µm 186003130 186003188 186003158 1860033413.0 x 20 mm Guard 5 µm 1860030634 1860030814 1860030724 18600336743.0 x 30 mm Column 5 µm 186003111 186003189 186003159 1860033423.0 x 50 mm Column 5 µm 186003131 186003190 186003160 1860033433.0 x 100 mm Column 5 µm 186003132 186003191 186003004 1860033443.0 x 150 mm Column 5 µm 186003112 186003014 186003005 1860033453.0 x 250 mm Column 5 µm 186003133 186003192 186003161 1860033464.6 x 20 mm IS ™ Column 5 µm 186003134 186003193 186003162 1860033474.6 x 20 mm Guard 5 µm 1860030644 1860030824 1860030734 18600336844.6 x 30 mm Column 5 µm 186003135 186003194 186003163 1860033484.6 x 50 mm Column 5 µm 186003113 186003015 186003006 1860033494.6 x 75 mm Column 5 µm 186003114 186003195 186003007 1860033504.6 x 100 mm Column 5 µm 186003115 186003016 186003008 1860033514.6 x 150 mm Column 5 µm 186003116 186003017 186003009 1860033524.6 x 250 mm Column 5 µm 186003117 186003018 186003010 186003353


10 x 10 mm Guard 5 µm 1860029721 1860029911 1860029831 18600335410 x 50 mm Column 5 µm 186002973 186003264 186003257 18600327110 x 100 mm Column 5 µm 186003255 186003265 186003258 18600327210 x 150 mm Column 5 µm 186002974 186003266 186003259 18600327310 x 250 mm Column 5 µm 186003256 186003267 186003260 18600327419 x 10 mm Guard 5 µm 1860029752 1860029922 1860029842 186003355OBD™ 19 x 30 mm Column 5 µm 186002976 186003268 186003261 186003275OBD™ 19 x 50 mm Column 5 µm 186002977 186002993 186002985 186003356OBD™ 19 x 100 mm Column 5 µm 186002978 186002994 186002986 186003357

OBD™

19 x 150 mm Column 5 µm 186002979 186002995 186002987 186003358OBD™ 30 x 50 mm Column 5 µm 186002980 186002996 186002988 186003277OBD™ 30 x 75 mm Column 5 µm 186002981 186003269 186003262 186003278OBD™ 30 x 100 mm Column 5 µm 186002982 186002997 186002989 186003279OBD™ 30 x 150 mm Column 5 µm 186003284 186003083 186002990 186003276

XBridge™ Analytical Columns

XBridge™ Preparative Columns

1 Requires 10 x 10 mm Prep Guard Holder 2890007792 Requires 19 x 10 mm Prep Guard Holder 1860007093 Requires Universal Sentry™ Guard Holder - 2.1 x 10 mm WAT0979584 Requires Universal Sentry™ Guard Holder - 3.0 x 20 mm/4.6 x 20 mm WAT046910


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©2005 Waters Corporation. Waters, XBridge, OBD, HPT, BEH Technology, ACQUITY UPLC, Atlantis,

SunFire, XTerra, Symmetry, IS, AutoPurification and Alliance are trademarks of Waters Corporation.

Luna and Gemini are trademarks of Phenomenex, Inc., ACE is a trademark of ACT.Inertsil is a trademark of GL Sciences.

Zorbax and Zorbax Extend are trademarks of Agilent Technologies. Capcell is a trademark of Shiseido Co., Ltd.

YMC is a trademark of YMC , Co., Ltd. All other trademarks are acknowledged.

June 05, 720001255EN

The quality management system of Waters’ manufacturing facilitiesin Taunton, Massachusetts and Wexford, Ireland complies with theInternational Standard ISO 9001:2000 Quality Management andQuality Assurance Standards. Waters’ quality management system isperiodically audited by the registering body to ensure compliance.

Austria and European Export (Central South Eastern Europe, CISand Middle East) 43 1 877 18 07

Australia 61 2 9933 1777

Belgium 32 2 726 1000

Brazil 55 11 5543 7788

Canada 1 800 252 4752 x2205

China 86 10 8451 8918

CIS/Russia 7 095 336 7000

Czech Republic 420 2 617 1 1384

Denmark 45 46 59 8080

Finland 358 9 506 4140

France 33 1 3048 7200

Germany 49 6196 400600

Hong Kong 852 29 64 1800

Hungary 36 1 350 5086

India and India Subcontinent

91 80 2837 1900

Ireland 353 1 448 1500

Italy 39 02 27 421 1

Japan 81 3 3471 7191

Korea 82 2 820 2700

Mexico 52 55 5524 7636

The Netherlands 31 76 508 7200

Norway 47 6 384 60 50

Poland 48 22 833 4400

Puerto Rico 1 787 747 8445

Singapore 65 6278 7997

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United Kingdom 44 208 238 6100

All other countries:Waters Corporation U.S.A.1 508 478 20001 800 252 4752

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XBridge Columns

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