X Ray Microscopy

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An easy approach to X-ray microscopy and 3D tomography

Transcript of X Ray Microscopy

Microscopía de Rayos-XTomografía 3D (BESSY)

Microscopio de rayos X

1 - 10 nm

(Alta resolución)

100 - 1000 eV

(Buena penetración)

Rayos - X Blandos

Magnificación

Óptico Rayos-X SEM

¿De dónde salen los rayos X?

Microscopio de Transmisión de rayos-X

(TXM)BESSY

Tomografía 3D de célulasutilizando TXM

BESSY

¿Por qué rayos X blandos?

2,34 - 4,38 nm

280 - 530 eV

CONTRASTE

AGUA-PROTEÍNA

Configuración del experimento

Análisis de los datos

Propagación lineal de los Rayos-X

1 px = 1 proyección

LAC

Resultado

Drosophila Melanogaster

Chlamydomonas reinhardtii

Referenciashttp://www.xradia.com/Technology/xraymicroscopy.html

http://xray1.physics.sunysb.edu/research/intro.php

http://en.wikipedia.org

D.Weiss, G. Schneider, B. Niemann, P. Guttmann, D. Rudolph and G. Schmahl, Ultramicroscopy 84 (2000), 185-197

G. Schneider, E. Anderson, S. Vogt, S. Knöchel, D. Weiss, M. Legros, and C. Larabell, “Computed tomography of cryogenic cells” Surface Review and Letters 9 (2002), 177-183

G. Schneider, P. Guttmann, S. Heim, S. Rehbein, D. Eichert, B. Niemann: X-Ray Microscopy at BESSY: From Nano-Tomography fo Fs-Imaging, SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation instrumentation (Eds.: J.-Y Choi, S. Rah), AIP Conference Proceedings 879 (2007), 1291-1294

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