World Renowned HPLC Media - Microsoft · App ID 23439 App ID 23441 App ID 23440 Intensity, cps...

1

OMEGA

World Renowned

HPLC MediaReinvented for UHPLC!

New Luna Omega 1.6 µm • Staggering UHPLC Efficiencies and Performance

• Extreme Ruggedness and Mechanical Strength

• Perfect Complement to Kinetex® Core-Shell Technology

Two Superb New Polar Selectivities for UHPLC and HPLC5

µm 3 µm 1.6

µmPolar C18 (1.6 µm, 3 µm, 5 µm)

PS C18 (1.6 µm, 3 µm, 5 µm)

2

Enhanced with 20 Years of Technology,

Innovation, and Experience

One of the world’s leading HPLC brands, now enhanced for incredible UHPLC performance! Luna Omega 1.6 µm UHPLC columns culminate 20 years of technological prowess, advancements, and innovation from Phenomenex!

With astounding efficiency levels, highly versatile selectivities, and trusted accuracy, Luna Omega columns will take your UHPLC

experience to a new level.

Luna® Omega UHPLC columns will boost your UHPLC instrumentation!

3

Cutting-Edge UHPLC ........................................................................... p. 4

Astounding Performance ...................................................................... p. 5

Separation Muscle ............................................................................... p. 6

Inert Foundation ................................................................................... p. 7

Excellent Reproducibility ................................................................. pp. 8-9

C18 Selectivity ................................................................................... p. 10

Utilizing Hydrophobicity ..................................................................... p. 11

Polar C18 Selectivity .......................................................................... p. 12

Enhanced Polar Retention ................................................................. p. 13

100% Aqueous Stability ..................................................................... p. 14

Polar and Non-polar Selectivity .......................................................... p. 15

Upgrading C18 Methods .................................................................... p. 16

Improving Existing Polar Methods ...................................................... p. 17

Polar Case Study: Catecholamines and PMETs .................................. p. 18

Polar Case Study: ETG/ETS ............................................................... p. 19

PS C18 Selectivity ................................................................................ p. 20

Enhanced Retention of Acids ............................................................... p. 21

Better Peak Shape for Bases ............................................................... p. 22

Excellent Loadability ............................................................................ p. 23

Greater Method Flexibility .................................................................... p. 24

Improving Existing Basic Compound Methods ..................................... p. 25

Use Luna and Luna Omega Phases Together ....................................... p. 26

UHPLC to HPLC to PREP Scalability.................................................... p. 27

C18 and Polar C18 - Complementary Work Horses ....................... pp. 28-29

Pairing With Core-Shell Technology .............................................. pp. 30-31

Column Lifetime ................................................................................... p. 32

Sample Preparation .............................................................................. p. 33

UHPLC Column Protection – Guard Cartridge System ......................... p. 34

HPLC Column Protection – Guard Cartridge System ............................ p. 35

Ordering Information ..................................................................... pp. 36-39

Why Luna® Omega Should be in Your Lab?

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© 2017 Phenomenex, Inc. All rights reserved.

Phenomenex l WEB: www.phenomenex.com

simonl

Sticky Note

Unmarked set by simonl

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Within the novel manufacturing process of Luna Omega 1.6 µm silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity.

Luna® is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, reproducibility and dependability for a wide range of analyses. The new Luna Omega 1.6 μm builds upon this legacy with an innovative yet rugged UHPLC silica particle architecture, designed and manufactured by Phenomenex based on more than 20 years of applied knowledge, invention, and customer experience.

Novel Design and Manufacturing Process

Cutting-Edge 1.6 μm Silica Particle

TMSTMS

C18 Polar C18

TMSTMS

PolarPolar

PS C18

TMSTMS

++

Thermal Modified Pore Structure

Most importantly, through our proprietary process, we eliminate micropores, further improving column efficiency, inertness, and reproducibility.

Consistent Porosity

Absence of Micropores


5

The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hy-brid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.

UHPLC Efficiency Comparison

Efficiency (plates/meter)

Luna® Omega 1.6 µm C18

Thermo Scientific® Hypersil GOLD® 1.9 µm C18

Agilent® ZORBAX® Eclipse 1.8 µm XDB-C18

Thermo Scientific Syncronis™ 1.7 µm C18

Waters® ACQUITY® HSS 1.8 µm C18

Waters ACQUITY BEH 1.7 µm C18

AkzoNobel® Kromasil® 1.8 µm C18

ACE® Excel™ 2 µm C18

YMC®-Triart 1.9 µm C18

Agilent ZORBAX 1.8 µm SB-C18

0 50,000 100,000 150,000 200,000 250,000 300,000 350,000

Conditions for all columns:Dimension: 50 x 2.1 mm

Mobile Phase: Acetonitrile/Water (65:35)Flow Rate: 0.5 mL/min

Temperature: AmbientDetection: UV @ 254 nm

System: ACQUITY UPLC®

Astounding PerformanceCutting-Edge 1.6 μm Silica Particle

Comparative separations may not be representative of all applications.


6

01.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e6

1 2 3 4 5 6 min

23441

1 2 3 4 5 6 min0.0

1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e6

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e56.0e56.5e57.0e57.5e5

1 2 3 4 5 6 min0.0

23440

Conditions for all columns:Columns: Luna Omega 1.6 µm C18

ZORBAX 1.8 µm XDB-C18ACE Excel 2 µm C18-AR

Dimension: 50 x 2.1 mmMobile Phase: A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in MethanolGradient: Time (min)

07

% B390

Flow Rate: 0.3 mL/minTemperature: 30 °C

Detection: MS/MSSample: 1. Succinic acid

2. MMA3. Glutaric acid4. Methylsuccinate5. Ethylmalonic acid6. Hippuric acid7. Homovanillic acid8. Suberic acid


ACE® Excel™ 2 µm C18-AR

Agilent® ZORBAX® 1.8 µm XDB-C18

Ap

p ID

234

39A

pp

ID 2

3441

Ap

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234

40

Inte

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Separation Muscle

Much Greater Resolution!

Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent station-ary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.



7

0.10

0

0.20

0.30

0.40

0.50

0.60

min0 1

1

2

4

3

5

6

2 3 4

Inert Foundation

Waters® ACQUITY® BEH 1.7 µm C18



ACQUITY BEH 1.7 µm C18Hypersil GOLD 1.9 µm C18

Dimension: 50 x 2.1 mmMobile Phase: A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in AcetonitrileGradient: Time (min)

056

6.18

% B5959555

Flow Rate: 0.4 mL/minTemperature: Ambient

Detection: UV @ 254 nmSample: 1. Pindolol

2. Chlorpheniramine3. Nortripytline 4. 3-Methyl-4-nitrobenzoic acid5. 5-Methyl salicylaldehyde6. Hexanophenone


Poor Peak Shape

Separation Muscle

0.10

0

0.20

0.30

0.40

0.50

0.60

min0 1

1

2

3

4

5

6

2 3 4

Excellent Peak Shape

Ap

p ID

238

05A

pp

ID 2

3806

0.10

0

0.20

0.30

0.40

0.50

0.60

min0 1

1

2

4

3

5

6

2 3 4

Ap

p ID

238

07

Luna Omega UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than tra-ditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy.


Poor Peak Shape


8

Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure depend-ability and reproducibility.

Luna Omega C18 – Batch A

0

0.2

0.4

0.6

0.8

1

1.2

1.4

10 2 3 4 5 min

1

23 4

56

7

8

Ap

p ID

235

23

Luna Omega C18 – Batch B

0

0.2

0.4

0.6

0.8

1

1.2

1.4

10 2 3 4 5 min

1

23 4

56

7

8A

pp

ID 2

3523

Luna Omega C18 – Batch C

0

0.2

0.4

0.6

0.8

1

1.2

1.4

10 2 3 4 5 min

1

23 4

56

7

8

Ap

p ID

235

23


Dimension: 50 x 2.1 mmPart No.: 00B-4742-AN

Mobile Phase: A: WaterB: Acetonitrile

Gradient: Time (min)06

6.018

% B20602020


Detection: UV @ 220 nmSample: 1. Estriol

2. Prednisolone3. Hydrocortisone4. Cortisone5. Cortisone Acetate6. 21-Hydroxycortisone7. 17-Hydroxycortisone8. Deoxycorticosterone

Peak Capacity

270

Peak Capacity

263

Peak Capacity

270

Consistent ResultsC18


9

Just like Luna® Omega C18, the Luna Omega Polar C18 is also stringently tested to make sure both batch-to-batch and column-to-column quality stays at the highest level before it reaches your lab bench.

Luna Omega Polar C18 – Batch A

25

0

50

75

100

120

100

150

175

10 2 3 4 min

12

34

5 6

7

Ap

p ID

235

70

Luna Omega Polar C18 – Batch B

25

0

50

75

100

120

100

150

175

10 2 3 4 min

1 2

34

5 6

7A

pp

ID 2

3570

Luna Omega Polar C18 – Batch C

25

0

50

75

100

120

100

150

175

10 2 3 4 min

1 2

34

5 6

7

Ap

p ID

235

70

Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18


Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile

Gradient: Time (min)0 5

% B595


Detection: UV @ 254 nmSample: 1. Uracil

2. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-nitrobenzoic acid 6. 5-Methyl salicylaldehyde7. Hexanophenone

Peak Capacity

163

Peak Capacity

166

Peak Capacity

162

QUALITYMANAGEMENT SYSTEMCERTIFIED BY DNV GL

9001:2008

Consistent ResultsPolar C18


10

Selectivity Highlight Luna Omega C18

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Luna® Omega C18 is an excellent first choice for chromatographers who are just starting method de-velopment or attempting to improve upon existing chromatographic results with other C18s. With its higher performance potential, excellent retention profile, and greater inertness, the Luna Omega C18 was designed to be the new all-purpose UHPLC solution for industries all over the world.

Phase C18

Particle Size 1.6 µm

Pore Size 100 Å

pH Range 1.5 - 8.5*

Surface Area 260 m2/g

Carbon Load 11 %

Pressure Limit 1000 bar

USP Listing L1

Greater Retention and Better ResultsHigher efficiency levels in combination with excellent stationary phase coverage and greater particle inert-ness, translates to improved separation power for you. Now you can utilize the greater retention of Luna Omega C18 to tackle both easy and difficult separations.

Luna Omega 1.6 µm C18


min0 0.5 1 1.5 2 2.5

AU

0

11

2 3

4

5 6

7

9

8

min0 0.5 1 1.5 2 2.5

AU

0

1

12

3

4

5 6

7

9

8

Ap

p ID

234

85

Ap

p ID

234

82

*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.

TMSTMS

Conditions for all columns:Columns: Luna Omega 1.6 μm C18

ACQUITY BEH 1.7 μm C18Dimension: 50 x 2.1 mm


Gradient: Time (min)0 3

3.5 3.51

5

% B10 55 55 10 10


Detection: UV @ 205 nmSample: 1. Acetaminophen

2. 4-Aminobenzoic Acid3. 4-Hydroxybenzoic Acid4. 2-Acetaminophenol5. 3-Hydroxybenzoic Acid6. Salicylicamide7. Phenol8. Benzoic Acid9. Salicylic Acid

Peak Capacity

196

Peak Capacity

160

Greater Peak Capacity and Retention



11

Small and Large Compound Mixtures

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18

Phenols

OTC Drug - Pill Formulation Profile

Synthetic Cannabinoids

Peptide Map – Digested BSA

AU

-0.005

0

0.005

0.01

0.015

0.02

0.025

min0 1

1

2

2

3

3

45

67

4 50.0

1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e6

3 4 5 6 7 8 9 min

Ap

p ID

234

42

Columns: Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm

Part No.: 00B-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water


067

7.19

% B5505055


Detection: UV @ 270 nmSample: 1. 3-Hydroxyphenol

2. Phenol3. 4-Nitrophenol4. 4-Methylphenol5. 2-Methylphenol6. 2,4-Dimethylphenol7. 1-Naphthol


Part No.: 00B-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water


06

% B50100


Detection: MS/MS (SCIEX API 4000™)Sample: 1. JWH-073 Butanoic acid metabolite

2. JWH-073 3-hydroxybutyl metabolite 3. JWH-018 Pentanoic acid metabolite 4. JWH-073 4-hydroxybutyl metabolite 5. JWH-018 4-hydroxypentyl metabolite 6. AM-2201 4-hydroxypentyl metabolite 7. JWH-018 5-hydroxypentyl metabolite 8. AM-694 9. AM-220110. JWH-073 11. JWH-018

Ap

p ID

234

34

0.01

0.02

0.03

0.04

0

AU

0 1 2 3 4 min0.00

1.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e91.10e9

10 20 30 min

Ap

p ID

236

00


Part No.: 00B-4742-ANMobile Phase: A: 20 mM Potassium Phosphate pH 7.2

B: AcetonitrileGradient: Time (min)

056

6.18

% B5707055


Detection: UV @ 254 nmSample: OTC Drug Pill


Part No.: 00D-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water


050

50.1

% B3503


Detection: MS/MS (SCIEX API 4000)Sample: Tryptic digest of BSA

Ap

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235

17

Inte

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, cp

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Strong and focused hydrophobic retention, incredible efficiency and valuable inertness of Luna® Omega C18 columns make them an excellent choice for small mixtures of compounds differing in hydrophobicity as well as large mixtures of compounds like impurity/degradation profiles and peptide maps.



12

Selectivity Highlight Luna Omega Polar C18

Luna® Omega Polar C18 is a novel UHPLC station-ary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non-polar analytes. The all-pur-pose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides en-hanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18 an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.

Phase Polar C18

Particle Size 1.6 µm, 3 µm, 5 µm

Pore Size 100 Å

pH Range 1.5 - 8.5*


Carbon Load 9 %


USP Listing L1

A C18, But DifferentLuna Omega Polar C18 is a uniquely modified C18-based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize UHPLC for mixtures of polar and non-polar compounds.

Luna Omega C18 silica surface

TMSTMS

Luna Omega Polar C18 silica surface

TMS

TMS


PolarPolar

TMSTMS

PolarPolar

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Greater Polar Retention

With its polar modified surface, the Luna® Omega Polar C18 offers UHPLC users enhanced separation pow-er that can greatly improve resolution values for target compounds.

Nicotine and Metabolites

Catecholamines

02.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e6

1+2 3

4

5

2 min1

1.0e5

2.0e5

3.0e64.0e6

5.0e6

6.0e67.0e6

8.0e6

02 min

2

3 4

5

1

1

2.0e54.0e56.0e58.0e61.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e6

02 min1

1+23

4

5

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

8.0e6

02 min

2

34

5

1

1

Ap

p ID

235

95A

pp

ID 2

3596


ACQUITY BEH 1.7 µm C18Dimension: 100 x 2.1 mm

Mobile Phase: A: Water with 0.1% Formic AcidB: Acetonitrile with 0.1% Formic Acid


3.1

% B0900


Detection: MS/MS (SCIEX API 4000)Sample: 1. Norepinephrine

2. Epinephrine3. Normetanephrine4. Dopamine5. Metanephrine

Ap

p ID

235

94A

pp

ID 2

3591

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CORTECS 1.6 µm C18Dimension: 50 x 2.1 mm

Mobile Phase: A: 10 mM Ammonium Formate with 0.1% Formic AcidB: Acetonitrile with 0.1% Formic Acid


3.1

% B2902


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Nornicotine

2. 3-Hydroxycotinine3. Nicotine4. Cotinine5. Anabasine

Greater Retention

Greater Retention

Luna Omega 1.6 μm Polar C18

Waters® CORTECS®

1.6 µm C18


Luna Omega 1.6 µm Polar C18

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100 % Aqueous Stability

No Stationary Phase CollapseTraditional C18 phases are known to collapse under 100% aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technol-ogy was used for the Luna® Omega Polar C18 in order to ensure aqueous stability. The graph below displays the excellent stability of Polar C18 in 100% aqueous buffer conditions for over 2 weeks.



Mobile Phase: 10 mM Ammonium Formate with 0.1 % Formic Acid

Flow Rate: 0.4 mL/min

Temperature: 22 °CDetection: UV @ 254 nm

Sample: 1. Adenine2. Guanosine-5-Monophosphate3. Thymine

Aqueous Stability of Luna Omega Polar C18

% o

f Ini

tial R

eten

tion

Tim

e

Adenine G-5-MP Thymine

100%110%

80%

60%

40%

20%

0%

Day 1

Day 25

Nucleosides in 100 % Aqueous Conditions



Mobile Phase: 20 mM Ammonium Formate pH 3.0Flow Rate: 0.4 mL/min

Temperature: 22 °C

Detection: UV @ 285 nmSample: 1. Thiourea

2. 5-Fluorocytosine3. Guanosine-5-Monophosphate4. Adenine5. Thymine

0.080

0.0100.020

0.030

0.040

0.050

0.060

0.070

1

2

3 4 5

min0 1 2

0

0.080

0.0100.020

0.030

0.040

0.050

0.060

0.070

1

2

34 5

min0 1 2

0

23585

Ap

p ID

235

85

Ap

p ID

235

86

Ap

p ID

235

77

Use 100% aqueous conditions for the greatest polar acid/base retention.



Duration of Exposure

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15

0

20

40

60

80

100

0

20

40

60

80

100

0

20

40

60

80

100

0

20

40

60

80

100

0 2 4 6 8 10 min

RT: 0.00 - 12.00

RT: 2.97AA: 96610SN: 1654

RT: 1.84AA: 7670SN: 36

RT: 1.93AA: 508SN: 4

RT: 2.68AA: 384SN: 10

RT: 8.23AA: 847097SN: 2491

RT: 9.77AA: 313SN: 10

RT: 7.62AA: 3628908SN: 4156

RT: 9.17AA: 167SN: 13

NL: 7.27E3TIC F: + c ESI

SRM ms2115.000

[98.099-98.101]MS ICISTest03b


SRM ms2166.100

[149.099-149.101]MS ICIS Test03b


SRM ms2171.100

[125.099-125.101]MS ICIS Test03b

RT: 1.32AA: 373SN: 34

RT: 4.64AA: 179SN: 35


SRM ms2416.000

[194.099-194.101]MS ICIS Test03b

100 % Aqueous Stability Versatility – Hydrophobic and Polar Interactions

Combined retention of polars and non-polars by the Luna® Omega Polar C18 gives you an incredible range of potential success when working with methods from just about any industry. Here we illustrate the useful-ness of these interaction mechanisms with water soluble vitamins from drink and tablet, a multi-compound antibiotic screen, and a UHPLC/MS/MS separation of algal toxins.

Antibiotic Screen

Find complete method at Phenomenex.comApplication ID: 23569

1.0e6

2.0e6

3.0e6

4.0e6

min0 1 2 3 4 50

Ap

p ID

235

87



Mobile Phase: A: 10 mM Ammonium Formate with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


4.17

% B09000


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Pyridoxamine

2. Thiamine3. Nicotinic acid4. Pyridoxine

5. Pantothenic acid6. Folic acid7. Riboflavin

Inte

nsity

, cp

s

2.0e5

1.0e5

3.0e5

5.0e56.0e5

4.0e5

2 3 4 min10

2 3

4

5

1.0e6

2.0e6

3.0e6

4.0e6

4.5e6

2 3 4 min10

2

4

5

6 71

Ap

p ID

235

78A

pp

ID 2

3579

Inte

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, cp

s

Rel

ativ

e A

bun

dan

ce

Inte

nsity

, cp

s

02.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e6

2

2

3

3

4

4

56

7

min1

1

Ap

p ID

235

84

Standards

Uracil-d4

Vitamin Enriched Drink

Anatoxin-a

Vitamin Tablet

L-Phenylalanine-d5

Cylindrospermopsin

Columns: Luna Omega 1.6 µm Polar C18Dimension: 50 x 2.1 mm

Part No.: 00B-4748-ANMobile Phase: A: 10 mM Ammonium Formate with

0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


0.55

5.1

% B09500


Detection: MS/MS (SCIEX API 4000)

Water Soluble Vitamins Algal Toxins (EPA 545)

1. Sulfaguanidine 2. Sulfadiazine 3. Cefalexin 4. Lincomycin 5. Amoxicillin 6. Sulfathiazole 7. Sulfapyridine 8. Cefazolin 9. Sulfamerazine 10. Ciprofloxacin 11. Sulfamethazine 12. Sulfamonomethoxine 13. Enrofloxacin 14. Difloxacin 15. Chlortetracycline 16. Sulfamethoxazole 17. Florfenicol 18. Sulfaquinoxaline 19. Oxacillin 20. Sulfadimethoxine

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16

Highly Useful Alternative to the Common C18

Unlike traditional UHPLC C18 stationary phases, the polar and hydrophobic versatility of Polar C18 com-bined with its high efficiency levels allow for enhanced separation power. Combine that with 100 % aqueous stability and you can really see how the Luna® Omega Polar C18 can potentially lead to more separation success compared to traditional C18 columns.

Ketotifen Impurity Profile

Beta Blockers

0.005

0.010

0.015

0.020

0.025

0.030

0

2 3 4 5 min1

0.010

0.015

0.020

0.025

0.030

0.035

02 3 4 5 min1

0.005

2

3

45

6

1

0.005

0.010

0.015

0.020

0.025

0.030

0

2 3 4 5 min1

0.005

0.010

0.015

0.020

0.025

0.030

0.010

0.015

0.020

0.025

0.030

0.035

0

0.005

2

2

3

3

4

4

5

56

min1

1

Ap

p ID

236

05A

pp

ID 2

3612


Hypersil GOLD 1.9 µm C18Dimension: 50 x 2.1 mm

Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


2.5610

10.0113

% B515203055


Detection: UV @ 254 nmSample: Ketotifen


ZORBAX 1.8 µm SB-C18Dimension: 100 x 2.1 mm

Mobile Phase: A: 20 mM Postassium Phosphate pH 7.2B: Acetonitrile


2.5

% B535


Detection: UV @ 280 nmSample: 1. Atenolol

2. Pindolol3. Timolol4. Metoprolol5. Labetolol6. Propranolol

Ap

p ID

236

04A

pp

ID 2

3611

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

sIn

tens

ity, c

ps




Agilent® ZORBAX® 1.8 µm SB-C18

Clean, sharp peaks and excellent resolution

Greater retention and resolution of impurities

Lu

na

Om

eg

a P

ola

r C

18



17

Excellent Alternative to Existing Polar Columns

Luna® Omega Polar C18 is an incredibly high performing UHPLC column with a unique selectivity profile that can be used to upgrade existing methods or jumpstart new method development. Realize lower limits of detection with the increase in sensitivity levels or just utilize the efficiency gains to help resolve closely eluting peaks.

Mycotoxins Screen

SAMHSA Workplace Drug Testing Screen

2.0e5

3.0e5

4.0e5

5.0e5

6.0e5

1.0e5

0

1

3+4

256

78

1 2 3 4 5 6 min

2.0e5

3.0e5

4.0e5

5.0e5

6.0e5

1.0e5

01 2 3 4 5 6 min

23571

2

3+4

5

67 8

1

2.0e5

0

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

1.6e61.8e6

1 2 3 4 min

23589

2.0e5

0

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

1.6e61.8e6

23588

1 2 3 4 min

Ap

p ID

235

72

Ap

p ID

235

88

Ap

p ID

235

89

Conditions same for all columns:Columns: Luna Omega 1.6 µm Polar C18

ACQUITY HSS 1.8 µm T3Dimension: 50 x 2.1 mm



3.1

% B1510010015


Detection: MS/MS (SCIEX API 4000)Sample: 1. Morphine

2. Codeine 3. Amphetamine 4. MDA 5. Methamphetamine 6. 6-MAM 7. MDMA 8. MDEA 9. BZE 10. PCP 11. THC-COOH 12. THC

Conditions same for all columns:Columns: Luna Omega 1.6 µm Polar C18

ACQUITY HSS 1.8 µm T3Dimension: 50 x 2.1 mm



4.16

% B20902020


Detection: MS/MS (SCIEX API 4000™)Sample: Positive Mode (ESI+)

1. 15-Acetyldeoxynivalenol2. Aflatoxin G23. Aflatoxin G14. Aflatoxin B25. Aflatoxin B16. Aflatoxin M17. HT2 Toxin8. T2 Toxin

Ap

p ID

235

71

Inte

nsity

, cp

s

Inte

nsity

, cp

s

Inte

nsity

, cp

s


Waters® ACQUITY® HSS 1.8 µm T3


Waters® ACQUITY® HSS 1.8 µm T3

Lu

na

Om

eg

a P

ola

r C18



18

Current analytical tests for pheochromocytoma (tumor of chromaffin cells of the adrenal medulla) focus on measuring for elevated levels of plasma free metanephrine and normetanephrine, which are continually se-creted by the tumors. Supplementary methods also analyze the metabolites of metanephrine and normeta-nephrine, including vanillylmandelic acid. With such a range of polar compounds, the aqueous stability and enhanced polar retention of the Luna® Omega Polar C18, both play an strong part in generating excellent separation between all these important analytes.

Catecholamines (ESI+)

PMETs from Plasma (ESI+)

Catecholamine Metabolites (ESI-)Flow Rate: 0.3 mL/min

Ap

p ID

235

91A

pp

ID 2

3592

Ap

p ID

235

93

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

8.0e6

02

2

3

3 4

5

6

4 min1

1

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

8.0e6

02 3

34

5

6

9

4 min1

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

02

8

7

9

3 min1

Conditions for all columns unless otherwise noted:Columns: Luna Omega 1.6 µm Polar C18

Dimension: 100 x 2.1 mmPart No.: 00D-4748-AN



3.1

% B0900


Detection: MS/MS (SCIEX API 4000™)Sample: Positive Mode (ESI+)

1. Norepinephrine2. Epinephrine3. Normetanephrine4. Dopamine5. Metanephrine6. 3-MethoxytyramineNegative Mode (ESI-)7. Vanillylmandelic acid (VMA)8. 5-Hydroxyindoleacetic acid (5-HIAA)9. Homovanillic acid (HVA)

Recommended Sample Preparation MethodProduct: Strata®-X-CW Microelution 96-Well PlatePart No.: 8M-S035-4GA

Condition: 200 µL MethanolEquilibrate: 200 µL Water

Load Sample: 250 µL Plasma diluted with 250 µL WaterWash 1: 200 µL WaterWash 2: 200 µL 50:50 Acetonitrile/IPA

Dry for 1 minute at 10” Hg to remove excess wash solvent

Elute: 2 x 25 µL 85:15 Acetonitrile/Water with 2 % Formic acid

Lu

na

Om

eg

a P

ola

r C

18Polar Case StudyCatecholamines and Metabolites


19

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are metabolites of ethanol that are used by analytical labora-tories to detect recent ethanol ingestion. In this application, the Luna® Omega Polar C18 provides excellent separation between EtG and EtS, along with valuable separation from a urine interference peak and where other matrix suppression is common. Additionally, the high efficiency levels provided by the Luna Omega 1.6 µm allow for a highly sensitive, yet rapid method.

ETG and ETS from Urine Sample

Ap

p ID

235

97A

pp

ID 2

3597

Ap

p ID

235

97

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s

2000400060008000

0

1.0e41.2e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e43.2e43.4e43.6e4

min2 31

2

3

1

200100

0

400300

600500

800700

9001000

12001100

13001365

2 3 min1

3

1


Dimension: 100 x 2.1 mmPart No.: 00D-4748-AN

Mobile Phase: A: 10 mM Ammonium Formate with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


1.15

% B05000


Detection: MS/MS (SCIEX API 4000™)Sample: Negative Mode (ESI-)

1. Urine interference2. ETS3. ETG

Overlaid XIC

XIC to ETS

XIC to ETG

2000400060008000

0

1.0e41.2e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e43.2e43.4e43.6e4

min2 31

2

1

ETS

ETG

Lu

na

Om

eg

a P

ola

r C18

Polar Case StudyETG/ETS


20

Selectivity HighlightLuna Omega PS C18

Luna® Omega PS C18 is a unique mixed-mode sta-tionary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C18 contains a positive charge which aids in the reten-tion of acidic compounds through ionic interactions, while the C18 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups.

Phase PS C18

Particle Size 1.6 µm, 3 µm, 5 µm

Pore Size 100 Å

pH Range 1.5 - 8.5*


Carbon Load 9 %


USP Listing L1

A C18, But More PositiveLuna Omega PS C18 has been fine-tuned and manufactured by Phenomenex to provide a mixed selectivity that is highly useful for method development involving either combinations of polars and non-polars, or just one single compound class with small changes in functional groups.

Luna Omega C18 silica surface

TMSTMS

Luna Omega PS C18 silica surface

TMS

TMS


++

TMSTMS

++

+

Lu

na

Om

eg

a P

S C

18


21

Inte

nsity

, cp

s

Waters® ACQUITY® HSS 1.8 μm T3

1

1

23

4

5

6

7

8

9

2 3 4 5 6 min0

1.00e52.00e53.00e54.00e55.00e56.00e57.00e58.00e59.00e51.00e61.10e61.20e6

1 2 3 min0

1.0e5

2.0e5

3.0e5

3.6e5ACE® Excel ™ 2 μm C18-AR

Luna Omega 1.6 μm PS C18

1 2 3 min0

2.0e4

4.0e4

6.0e4

8.0e4

1.0e5

1.2e5

1.4e5

1.6e5

1.8e52.0e5

Enhanced Retention of Acids

With its positive surface (PS) the Luna® Omega PS C18 provides valuable in-crease in retention of acids through ionic/polar interactions. In addition, the C18 ligand also stimulates hydrophobic retention that greatly promotes increased resolution between analytes of interest.

MMA and Succinic Acid

Organic Acids

Ap

p ID

236

79A

pp

ID 2

3689

Conditions for all columns:Columns: Luna Omega 1.6 μm PS C18

ACQUITY HSS 1.8 μm T3Dimension: 100 x 2.1 mm



5.17

% B05000


Detection: MS/MS (SCIEX API 4000)Sample: 1. Lactic acid

2. Succinic acid3. MMA4. Glutaric acid5. Ethylmalonic acid6. Methyl succinate7. Hippuric acid8. Suberic acid9. Sebacic acid

Ap

p ID

236

77A

pp

ID 2

3686

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s


ACE Excel 2 μm C18-ARDimension: 50 x 2.1 mm



5.17

% B05000


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Succinic acid

2. MMA

Greater Retention and Resolution

Increased Resolution

Luna Omega 1.6 µm PS C18

1

1

2 3

4

5

6

7

8

9

2 3 4 5 6 min0

1.00e52.00e53.00e54.00e55.00e56.00e57.00e58.00e59.00e51.00e61.10e61.20e6

Lu

na

Om

eg

a P

S C

18



22

1 2 3 min0

5.0e4

1.0e5

1.5e5

2.0e5

2.5e5

3.0e5

3.5e5

4.0e5

4.5e5

5.0e5

5.5e55.7e5

0 1

1

2

3

45

6

7

2 3 4 5 min-0.050.000.050.100.150.200.250.300.350.400.450.500.55

AU

Waters® ACQUITY® BEH 1.7 μm C18


0 1

1

2

4

3

5

6

7

2 3 4 5 min-0.050.000.050.100.150.200.250.300.350.400.450.500.55

Better Peak Shape for Bases

While traditional alkyl phases are prone to show tailing for basic compounds because of secondary inter-actions occurring at the silica surface, the surface of the Luna® Omega PS C18 was designed with positive charges that serve to repel strong basic species and consistently display sharp peak shape.

Pharmaceutical Compound Mixture

Intact Insulin

Ap

p ID

237

14


Hypersil GOLD 1.9 μm C18ZORBAX 1.8 µm SB-C18

Dimension: 50 x 2.1 mmMobile Phase: A: Water with 0.1% Formic Acid

B: Acetonitrile with 0.1% Formic Acid


3.15

% B38033


Detection: MS/MS (SCIEX API 4000)Sample: Insulin

Ap

p ID

237

07

Ap

p ID

236

92, 2

3693

and

236

94

AU

Inte

nsity

, cp

s





5.18

% B59555


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Uracil

2. Pindolol3. Chlorpheniramine4. Nortriptyline5. 3-Methyl-4-nitrobenzoic acid6. 5-Methyl salicylaldehyde7. Hexanophenone

Better Peak Shape



Agilent® ZORBAX® 1.8 µm SB-C18


Lu

na

Om

eg

a P

S C

18

Better Peak Shape


23

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

1.5 2 2.5 min

AU

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

1.5 2 2.5 min

Excellent Loadability

The combination of great surface coverage and novel surface chemistry results in narrow peak shapes for an extended amount of sample loaded on the Luna® Omega PS C18. This can visually be seen in the examples below where the excellent peak shape at different loads is kept relatively constant on the Luna Omega PS C18, while more conventional UHPLC columns show tailing and much greater peak broadening as more sample is loaded.

Amitriptyline Loading Study

Ap

p ID

237

38





% B580


Detection: UV @ 254 nmSample: Amitriptyline

Ap

p ID

237

37

Inte

nsity

, cp

sA

U


Titan 1.9 µm C18HALO 2 µm C18

Dimension: 50 x 2.1 mmMobile Phase: Water with 0.1% Formic Acid / Acetonitrile with 0.1%

Formic Acid (78:22)Flow Rate: 0.5 mL/min


Sample: Amitriptyline

Better Peak Shape Over All Loads

Waters® ACQUITY® BEH 1.7 μm C18



Luna Omega PS C18 and Competitors 50 x 2.1 mm

Pea

k W

idth

at

50%

µg on column

Amitriptyline 1µg Load

min0 1 2 3

mAU

0

2

4

6

8

10

12

14

16

Ap

p ID

237

24


Supelco® Titan™ 1.9 µm C18

AMT HALO® 2 µm C18

0 0.1 0.2 0.3 0.4 0.5 0.6

0

0.09

0.08

0.07

0.05

0.06

0.04

0.03

0.02

0.01

Lu

na

Om

eg

a P

S C

18

Luna 1.6 µm PS C18

HALO 2 µm C18

ACQUITY BEH 1.7 µm C18

Hypersil GOLD® 1.9 µm C18

Ap

p ID

237

39

Better Peak Shape Over All Loads


24

0 1 2 3 4 5 6 7 8 9 10 min

0

0.05

0.10

0.15

0.20

0.25

0.30

0.35

0 1 2 3 4 5 6 7 8 9 10 min

0

0.05

0.10

0.15

0.20

0.25

0.30

0.35

0 1 2 3 4 5 6 7 8 9 10 min

0

0.05

0.10

0.15

0.20

0.25

0.30

0.35

Method Development Flexibility

The combination of available Luna® Omega stationary phases represent an outstanding tool set for the sepa-ration of acids, bases, neutrals or mixtures. Use the C18 to focus on hydrophobic interactions or the mixed-mode functionality of the Polar C18 and PS C18 to get enhanced retention of both polars and non-polars.

Pharmaceutical Drug Impurity Profile

Ap

p ID

237

41A

pp

ID 2

3743

Ap

p ID

237

44

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s


Luna Omega 1.6 μm Polar C18Luna Omega 1.6 μm C18

Dimension: 50 x 2.1 mmMobile Phase: A: Water with 0.1% Formic Acid

B: Acetonitrile with 0.1% Formic AcidGradient: Time (min)

010

% B540


Detection: UV @ 254 nmSample: Proprietary drug impurity profile


Luna Omega 1.6 μm C18


Lu

na

Om

eg

a P

S C

18

Useful alternative peak elution order


25


0 1 3 3 4 5 min-0.010

0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

0 1 3 3 4 5 min-0.010

0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

1 2 3 4 min0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e5

1 2 3 4 min0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e5

Peptides by LC/MS

Improving Existing Basic Compound Methods

Luna® Omega PS C18 is a brilliant UHPLC stationary phase with a distinctive selectivity profile that can spur new method development. At the same time, this versatile phase can also upgrade existing methods by way of increases in sensitivity and efficiency levels to help resolve closely eluting peaks.

Beta-Blockers


ACQUITY CSH 1.7 µm C18Dimension: 50 x 2.1 mm



3.15

% B39533


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Angiotensin II

2. Met-Enkephalin3. Leu-Enkephalin


CORTECS 1.6 μm C18+Dimension: 50 x 2.1 mm

Mobile Phase: A: 10 mM Ammonium Formate pH 3.0B: Acetonitrile


% B235


Detection: UV @ 280 nmSample: 1. Pindolol

2. Timolol3. Metoprolol4. Labetolol5. Propranolol

Inte

nsity

, cp

s


Ap

p ID

237

27

Inte

nsity

, cp

sIn

tens

ity, c

ps

Waters® ACQUITY® CSH 1.7 µm C18

Ap

p ID

237

36

Inte

nsity

, cp

s

Ap

p ID

237

03

1 2 3 4 min0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e5

Ap

p ID

237

02A

pp

ID 2

3701

Better Sensitivity

Luna Omega

ACQUITY CSH

Lu

na

Om

eg

a P

S C

18

Waters® CORTECS® 1.6 μm C18+



26

mAU

-2

0

2

4

6

8

10

12

14

min10 20 30 40 50 60

mAU

-2

0

2

4

6

8

10

12

14

min10 20 30 40 50 60

mAU

-2

0

2

4

6

8

10

12

14

min10 20 30 40 50 60

mAU

-2

0

2

4

6

8

10

12

14

min10 20 30 40 50 60

Use Luna and Luna Omega Phases Together

Combine the new Luna® Omega 5 µm Polar C18 and PS C18 with Luna 5 µm phases like Phenyl-Hexyl and PFP(2) to expand your HPLC method development choices or try different separation modes with Luna phases such as Silica(2), NH2, and HILIC.

Ezetimibe Degradation Profile

Ap

p ID

237

97A

pp

ID 2

3798

Ap

p ID

237

99A

pp

ID 2

3800

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

sIn

tens

ity, c

ps

Conditions for all columns:Columns: Luna 5 μm PFP(2)

Luna Omega 5 μm Polar C18Luna 5 μm Phenyl-HexylLuna 5 μm C18(2)

Dimension: 250 x 4.6 mmMobile Phase: A: Water


03760

% B323255

Flow Rate: 2 mL/minTemperature: 22 °C

Detection: UV @ 215 nmSample: Ezetimibe Degradation Profile

Luna 5 μm PFP(2)

Luna 5 μm Phenyl-Hexyl

Luna 5 μm C18(2)

Luna Omega 5 μm Polar C18


27

1 2 3 4 min

65

43

21

7

mAU

0

25

50

75

100

125

150

175

Direct Scalability 1.6 µm to 5 µm

UHPLC to HPLC to PREP Scalability

With direct selectivity scalability from Luna® Omega 1.6 µm to 5 µm you can fluidly transfer methods from UHPLC platforms to HPLC and preparative instrumentation. Additionally, you can easily go in reverse and use a Luna Omega 1.6 µm to analyze fractions taken from a Luna Omega 5 µm preparative column.

UHPLC to HPLC to PREP

Analyze Prep Fractions via

UHPLC

Conditions for all columns:Columns: Luna Omega 1.6 μm Polar C18

Luna Omega 5 µm Polar C18Dimension: 50 x 2.1 mm



% B595


Detection: UV @ 254 nmSample: 1. Uracil

2. Pindolol3. Chlorpheniramine4. Nortriptyline5. 3-Methyl-4-nitrobenzoic acid6. 5-Methyl salicylaldehyde7. Hexanophenone

Conditions for all columns (as noted):Columns: Luna Omega 5 μm PS C18

Mobile Phase: A: Water with 0.1% TFAB: Acetonitrile with 0.1% TFA


% B1090


Sample: 1. Impurity2. Proprietary API3. Impurity

Ap

p ID

237

45

Inte

nsity

, cp

s


Luna Omega 5 μm Polar C18

UHPLC Scout

Dimension: 50 x 2.1 mmFlow Rate: 0.2 mL/min

0 1 2 3 min-0.0020.0000.0020.0040.0060.0080.0100.0120.0140.0160.0180.020

0 1 2 3 min-0.0020.0000.0020.0040.0060.0080.0100.0120.0140.0160.0180.020

Preparative Purification

Dimension: 150 x 21.2 mmFlow Rate: 30 mL/min

min5 6 7 8

mAU

0

20

40

60

80

100

120

140

min5 6 7 8

mAU

0

20

40

60

80

100

120

140

Optimized HPLC

Dimension: 150 x 4.6 mmFlow Rate: 1.5 mL/min

min5 6 7 8

mAU

0

20

40

60

80

100

120

140

min5 6 7 8

mAU

0

20

40

60

80

100

120

140

Ap

p ID

237

55A

pp

ID 2

3757

Ap

p ID

237

56



28

Complementary UHPLC Work Horses

Influence Selectivity with 100 % Aqueous Stability and Polar Interactions

Intact PeptidesConditions for all columns except where noted:

Columns: Luna Omega 1.6 µm Polar C18Luna Omega 1.6 µm C18

Dimension: 50 x 2.1 mmMobile Phase: A: Water with 0.1 % TFA

B: Acetonitrile with 0.1 % TFAGradient: Time (min)

015

% B0065


Detection: UV @ 210 nmSample: 1. Gly-Tyr

2. Val-Tyr-Val3. Met-Enkephalin4. Leu-Enkephalin5. Angiotensin II

0

2 3 4 5 min1

200

400

600

mAU

0

2 3 4 5 min1

200

400

600

mAU

Ap

p ID

236

06

Ap

p ID

236

09

Use the 100 % aqueous stability of polar C18 to aid in better retention of polar compounds



Combine the versatile interaction mechanisms of the Luna® Omega C18 and Polar C18 to achieve success-ful separations and improve upon challenging existing methods.

Natural Cannabinoids

Ap

p ID

235

81


Luna Omega 1.6 µm C18Dimension: 100 x 2.1 mm

Mobile Phase: A: 20 mM Ammonium Formate pH 3.2B: Acetonitrile


13.0115

% B6095956060


Detection: UV @ 256 nmSample: 1. CBDV

2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. Delta 9 THC 8. Delta 8 THC 9. CBC 10. THCA-A

Ap

p ID

235

80

Inte

nsity

, cp

sIn

tens

ity, c

ps

0

4 6 7 8 9 105 min3

0.010

0.020

0.030

0.040

0.050

0.060

0.070mAU

0

4 6 7 8 9 105 min3

0.010

0.020

0.030

0.040

0.050

0.060

0.070mAU


Greater hydrophobic retention




29

Complementary UHPLC Work Horses

Conditions same for all columns:Columns: Luna Omega 1.6 µm C18

Luna Omega 1.6 µm Polar C18Dimension: 100 x 2.1 mm



50.1

% B3503


Detection: MS/MS (SCIEX API 4000™)Sample: Digested BSA

Inte

nsity

, cp

s

1.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e92.00e93.00e94.00e9

14 16 18 20 22 24 26 28 30 32 34 36 38 min2 4 6 8 10 12

Ap

p ID

235

99

Inte

nsity

, cp

s

0.001.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e91.10e9

10 20 30 min

Ap

p ID

236

00

Inte

nsity

, cp

s

Inte

nsity

, cp

s

018 19 min17

2.00e8

4.00e8

6.00e8

8.00e8

10.00e8

12.00e8

Ap

p ID

236

01

Ap

p ID

236

01


Luna Omega 1.6 µm

Polar C18



Greater resolution of early, more polar eluters

Better resolution of more hydrophobic compounds

018 19 min17

2.00e8

4.00e8

6.00e8

8.00e8

10.00e8

12.00e8

For the analysis of a large screen or mixture of compounds, you can benefit greatly from utilizing both the Luna® Omega C18 and Polar C18 during method development. The strong focused hydrophobic retention of the C18 will provide excellent non-polar retention and separation, while the Polar C18 provides a complementary hydro-phobic/polar combined selectivity that can retain and separate typically early eluting compounds

Tryptic Digest - Peptide Map of BSA



30

Luna Omega + Kinetex = Happy UHPLC

Increase your method development options by screening a combination of core-shell and fully porous selectivities.

Natural Cannabinoids

Inte

nsity

, cp

sIn

tens

ity, c

ps

0

4 6 7 8 9 105 min3

0.010

0.020

0.030

0.040

0.050

0.060

0.070mAU

1 2 37 8

910

4

5

6

0.010

0.020

0.030

0.040

0.050

0.060

0.070

0

4 6 7 8 9 105 min3

mAU

178

9 10

2-4

5

6

Ap

p ID

235

82A

pp

ID 2

3580


Kinetex 1.7 µm BiphenylKinetex 1.7 µm F5

Dimension: 100 x 2.1 mmMobile Phase: A: 20 mM Ammonium Formate pH 3.2


01213

13.0115

% B6095956060


Detection: UV @ 256 nmSample: 1. CBDV

2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. Delta 9 THC 8. Delta 8 THC 9. CBC 10. THCA-A

Luna® Omega 1.6 µm Polar C18

Kinetex 1.7 µm Biphenyl

Biphenyl

TMSTMS

Inte

nsity

, cp

s

0

0.010

0.020

0.030

0.040

0.050

0.060

0.070

4 6 7 8 9 105 min3

mAU

1 2 3 7 8

910

4

5

6

Ap

p ID

235

83

Kinetex® 1.7 µm F5

F5

F

F

F

F

F

TMSTMS

Excellent resolution

Faster analysis with similar peak order

Alternative peak elution order

TMSTMS

PolarPolar



31

Conditions for all columns except where noted:Columns: Luna Omega 1.6 µm C18

Kinetex 1.7 µm BiphenylKinetex 1.7 µm EVO C18

Dimension: 50 x 2.1 mmMobile Phase: Luna Omega 1.6 µm C18 and Kinetex 1.7 µm Biphenyl

A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in AcetonitrileKinetex 1.7 µm EVO C18A: 10 mM Ammonium Bicarbonate pH 10B: Acetonitrile


5.1

% B595955


Detection: MS/MS (SCIEX API 4000™)Sample: Drugs of Abuse

Luna Omega + Kinetex =Happy UHPLC

Combining orthogonal UHPLC particle morphologies and selectivities like the Luna Omega C18, Kinetex Biphenyl, and Kinetex EVO C18 will drastically in-crease your probability of separation success!

Morphine

Drugs of Abuse Analysis Luna® Omega 1.6 µm C18

Kinetex® 1.7 µm Biphenyl

Kinetex 1.7 µm EVO C18A

pp

ID 2

3444

1 2 3 4 min

23443

1 2 3 4 min

1 2 3 4 min

Ap

p ID

234

43

pH 2.5

pH 2.5

pH 8.5

ADVANTAGE Excellent inertness and

strong hydrophobic retention

ADVANTAGE Extra aromatic (pi-pi)

and dipole-dipole retention

ADVANTAGE Excellent peak shape and

retention of bases at high pH

Ap

p ID

234

45



32

Excellent Lifetime

Luna Omega 1.6 µm media was designed to be durable and withstand the high pressures and rigors of UHPLC work in combination with providing excellent performance.

Accelerated Lifetime Study

% In

crea

se o

ver

Initi

al

Bac

kpre

ssur

e

Injection Cycle

Luna® Omega 1.6 µm C18 (AVG)

Waters® ACQUITY® BEH 1.7 µm C18 (AVG)

0 10 20 30 40 50 600.0%

1.0%

2.0%

3.0%

4.0%

5.0%

6.0%

7.0%

8.0%

9.0%

10.0%

Ap

p ID

236

10

Conditions same for both columns:Columns: Luna Omega 1.6 µm C18




4.1

% B5955


Detection: UV @ 210 nmSample: Protein Matrix

Extend UHPLC Column Lifetime Even More with SecurityGuard™ ULTRASee page 34 for details



33

Further Improve Lifetime with Sample Preparation Both UHPLC instrumentation and columns can benefit from consistent removal of microparticulates/contaminants that may appear in solvents, samples or spe-cific matrices. Over time these problematic compounds can build up on instru-mentation and columns, leading to the need for costly maintenance and repair. Strata-X polymeric SPE, Novum liquid extraction, and Phenex syringe filters are excellent options to try.

Convenient and inexpensive way to remove particulates from LC samples

www.phenomenex.com/Phenex

Very targeted form of sample preparation that allows you to isolate your analyte of interest while removing any interfering compounds in your sample

www.phenomenex.com/StrataX

Remove interferences such as proteins, phospholipids, salts, and more in a quick and simple procedure

www.phenomenex.com/Novum

Sample Preparation

novumsimplified liquid extraction

Polymeric SPEPolymeric SPE


34

Protect any UHPLC Column

See it in action: www.phenomenex.com/SecurityGuardULTRA

Secu

rityGu

ard™ U

LTR

AU

HP

LC C

olumn to D

etectorFrom

Injector

CartridgeHolder

SecurityGuard ULTRA Guard Cartridge System (Cartridge connected to Holder)

* Cartridge schematic not drawn to scale

Compounds of Interest

Contaminants and Particulates

Material Chamber

FritContaminants Trapped

ULTRAUHPLC Column Protection

Protect your UHPLC column, including Luna® Omega 1.6 µm columns, from damaging contaminants and microparticulates with the SecurityGuard ULTRA guard cartridge system!

• Simple to use

• Extend column lifetime

• Pressure rated to 20,000 psi (1,378 bar)

• Fits virtually all manufacturers’ columns 2.1 to 4.6 mm ID


35

Protect any HPLC Column

See it in action: www.phenomenex.com/SecurityGuard

Accelerated lifetime test using endogenous biomolecule matrix on a 5 µm 50 x 4.6 mm column with SecurityGuard cartridges. Backpressure values represent additional backpressure contributed by SecurityGuard.

The SecurityGuard analytical cartridge holder (pat-ented) directly finger-tightens into virtually any man-ufacturer’s column endfitting. Contaminants are retained by an inexpensive disposable cartridge instead of damaging your valuable HPLC and SFC column investment. Simply replace SecurityGuard cartridges instead of your expensive columns. In this graph, once the expired SecurityGuard cartridge was replaced, the pressure immediately dropped and the column performance was restored allowing for extended column use.

BG

uard

Cartrid

ge S

ystemH

PLC

Colum

n to Detector

From Injector

A

Contaminants Trapped

contaminants

compounds of interest

A

B

Cutaway view showing cartridge — can be easily inspected for contaminants

Universal Fingertight connection to HPLC column — no wrenches required

-10

0

10

20

30

40

50

60

70

80

90

100

110

Cartridge Replaced

Back

pres

sure

(Bar

)

Injections

Column returns to initial backpressure

HPLC Column Protection

The easiest way to extend column performance is to prevent contaminants and particulates from getting into your Luna® Omega 5 µm column with the SecurityGuard Standard guard cartridge system.

• Protects and extends column lifetimes

• Virtually no change in chromatography

• Simple to use

Fingertight connection to virtually any female inverted endfitting worldwide


36

Luna Phases Description

Particle Sizes (µm)

Pore Size (Å)

Surface Area

(m2/g)

Carbon Load (%)

pH Stability

Reversed Phase

Normal Phase HILIC IEX

Silica(2) Unbonded silica 3, 5, 10, 10-PREP,

15

100 400 — 2.0 - 7.5

C5 5 Carbon ligand 5, 10 100 440 12.5 1.5 - 9.0*

C8(2) C8 ligand optimized for improved peak shape

3, 5, 10, 10-PREP,

15

100 400 13.5 1.5 - 9.0*

C18(2) C18 ligand optimized for improved peak shape

2.5, 3, 5, 10,

10-PREP, 15

100 400 17.5 1.5 - 9.0*

CN Versatile CN phase 3, 5, 10 100 400 7.0 1.5 - 7.0

NH2 Rugged and reproducible NH2 3, 5, 10 100 400 9.5 1.5 - 11

Phenyl-Hexyl Phenyl phase attached to C6 (hexyl) ligand

3, 5, 10, 10-PREP,

15

100 400 17.5 1.5 - 9.0*

SCX Benzene sulfonic acid 5, 10 100 400 Binding Capacity:

0.15 meq/g

2.0 - 7.0

HILIC Reproducible, cross-linked diol 3, 5 200 200 5.7 1.5 - 8.0

PFP(2) Pentafluorophenyl with a C3 (propyl) linkage

3, 5 100 400 11.5 1.5 - 8.0

* pH range is 1.5 - 9 under gradient conditions. pH range is 1.5 - 10 under isocratic conditions.

Luna® Omega Phases Description

Particle Sizes (µm)

Pore Size (Å)

Surface Area

(m2/g)

Carbon Load (%)

pH Stability

Reversed Phase

Normal Phase HILIC IEX

C18 C18 ligand optimized for improved peak shape

1.6 100 260 11 1.5 - 8.5*

Polar C18 Enhanced selectivity/retention for polar analytes without diminishing useful non-polar retention

1.6, 3, 5 100 260 9 1.5 - 8.5*

PS C18 Mixed-mode functionality offering enhanced retention of polar acids along with improved peak shape for strong bases

1.6, 3, 5 100 260 9 1.5 - 8.5*

* pH stability under gradient conditions. pH stability is 1.5 - 10 under isocratic conditions.

Luna and Luna Omega Column Characteristics

Find more information on all these phases online at www.phenomenex.com/Luna


37

Ordering Information

Buy Luna® Omega Now

5 µm Minibore and MidBore™ Columns (mm) SecurityGuard

Cartridges (mm)Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 50 x 3.0 100 x 3.0 150 x 3.0 4 x 2.0*

/10 pkPolar C18 00A-4754-AN 00B-4754-AN 00D-4754-AN 00F-4754-AN 00B-4754-Y0 00D-4754-Y0 00F-4754-Y0 AJ0-7600PS C18 00A-4753-AN 00B-4753-AN 00D-4753-AN 00F-4753-AN 00B-4753-Y0 00D-4753-Y0 00F-4753-Y0 AJ0-7605

for ID: 2.0 - 3.0 mm

5 μm Analytical Columns (mm) SecurityGuard

Cartridges (mm)Phases 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0*

/10 pkPolar C18 00B-4754-E0 00D-4754-E0 00F-4754-E0 00G-4754-E0 AJ0-7601PS C18 00B-4753-E0 00D-4753-E0 00F-4753-E0 00G-4753-E0 AJ0-7606

for ID: 3.1-8.0 mm

5 μm Axia™ Packed Preparative Columns (mm)SecurityGuard

Cartridges (mm)Phases 150 x 21.2 250 x 21.2 150 x 30 250 x 30 250 x 50 15 x 21.2** 15 x 30.0♦

/ea /eaPolar C18 00F-4754-P0-AX 00G-4754-P0-AX 00F-4754-U0-AX 00G-4754-U0-AX 00G-4754-V0-AX AJ0-7603 AJ0-7604PS C18 00F-4753-P0-AX 00G-4753-P0-AX 00F-4753-U0-AX 00G-4753-U0-AX 00G-4753-V0-AX AJ0-7608 AJ0-7609

for ID: 21.2 mm for ID: 30 mm

‡ SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000

* SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-4282

** PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223

♦ PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8277

1.6 μm Minibore Columns (mm) SecurityGuard™

ULTRA Cartridges‡

Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 3/pk

Polar C18 00A-4748-AN 00B-4748-AN 00D-4748-AN 00F-4748-AN AJ0-9505PS C18 00A-4752-AN 00B-4752-AN 00D-4752-AN 00F-4752-AN AJ0-9508C18 00A-4742-AN 00B-4742-AN 00D-4742-AN 00F-4742-AN AJ0-9502

for 2.1 mm ID

3 µm Minibore and MidBore™ Columns (mm) SecurityGuard™

Cartridges (mm)Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 50 x 3.0 100 x 3.0 150 x 3.0 4 x 2.0*

/10 pkPolar C18 00A-4760-AN 00B-4760-AN 00D-4760-AN 00F-4760-AN 00B-4760-Y0 00D-4760-Y0 00F-4760-Y0 AJ0-7600PS C18 00A-4758-AN 00B-4758-AN 00D-4758-AN 00F-4758-AN 00B-4758-Y0 00D-4758-Y0 00F-4758-Y0 AJ0-7605

for ID: 2.0-3.0 mm

3 µm Analytical Columns (mm)SecurityGuard


/10 pkPolar C18 00B-4760-E0 00D-4760-E0 00F-4760-E0 00G-4760-E0 AJ0-7601PS C18 00B-4758-E0 00D-4758-E0 00F-4758-E0 00G-4758-E0 AJ0-7606

for ID: 3.1-8.0 mm

1.6 μm Microbore Columns (mm) Phases 50 x 1.0 100 x 1.0 150 x 1.0

Polar C18 00B-4748-A0 00D-4748-A0 00F-4748-A0C18 00B-4742-A0 00D-4742-A0 00F-4742-A0


If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, return the column with comparative data within 45 days FOR A FULL REFUND.

38


Buy Luna® Now

Find 2.5 µm and 3 µm Luna column options at www.phenomenex.com/Luna


5 μm Microbore and Minibore Columns (mm)SecurityGuard™ Cartridges (mm)

Phases 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0* /10 pk

Silica(2) — — — 00A-4274-B0 00B-4274-B0 00F-4274-B0 00G-4274-B0 AJ0-4347C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292C8 (2) — 00F-4249-A0 — 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289C18 (2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286CN — — — — 00B-4255-B0 00F-4255-B0 — AJ0-4304Phenyl-Hexyl 00B-4257-A0 — — 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350NH2 00B-4378-A0 00F-4378-A0 — 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301PFP(2) — — — 00A-4448-B0 00B-4448-B0 00F-4448-B0 — AJ0-8326

for ID: 2.0 - 3.0 mm

5 μm MidBore™ Columns (mm)SecurityGuard


/10 pkSilica(2) — 00B-4274-Y0 00F-4274-Y0 — AJ0-4347C5 — — 00F-4043-Y0 — AJ0-4292C8(2) 00A-4249-Y0 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 AJ0-4289C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 AJ0-4286CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 AJ0-4304Phenyl-Hexyl — 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 AJ0-4350NH2 — 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 AJ0-4301SCX — — 00F-4398-Y0 — AJ0-8307HILIC — — 00F-4450-Y0 — AJ0-8328PFP(2) — 00B-4448-Y0 00F-4448-Y0 — AJ0-8326

for ID: 2.0 - 3.0 mm

5 μm Analytical Columns (mm)SecurityGuard

Cartridges (mm)Phases 30 x 4.6 50 x 4.6 75 x 4.6 4 x 3.0*

/10 pkSilica(2) — 00B-4274-E0 — AJ0-4348C5 — 00B-4043-E0 — AJ0-4293C8(2) 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4290C18(2) 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4287CN 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4305Phenyl-Hexyl 00A-4257-E0 00B-4257-E0 — AJ0-4351NH2 — 00B-4378-E0 — AJ0-4302SCX — 00B-4398-E0 — AJ0-4308HILIC — — — AJ0-8329PFP(2) — 00B-4448-E0 — AJ0-8327

for ID: 3.2-8.0 mm*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282

39


Buy Luna® Now


5 μm Analytical and Semi-Prep Columns (mm) SecurityGuard™ Cartridges (mm)Phases 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 4 x 3.0* 10 x 10‡

/10 pk /3 pkSilica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 00G-4274-N0 AJ0-4348 AJ0-7223C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 00G-4043-N0 AJ0-4293 AJO-7372C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 AJ0-4290 AJ0-7222C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 AJ0-4287 AJ0-7221CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 AJ0-4305 AJ0-7313Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 AJ0-4351 AJ0-7314NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 AJ0-4302 AJ0-7364SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 AJ0-4308 AJ0-7369HILIC 00D-4450-E0 00F-4450-E0 00G-4450-E0 00G-4450-N0 AJ0-8329 AJ0-8902PFP(2) 00D-4448-E0 00F-4448-E0 00G-4448-E0 00G-4448-N0 AJ0-8327 AJ0-8376

for ID: 3.2 - 8.0 mm 9 - 16 mm

5 μm Axia™ Packed Preparative Columns (mm) SecurityGuard

Cartridges (mm)Phases 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 15 x 21.2**

/eaSilica(2) — 00D-4274-P0-AX 00F-4274-P0-AX 00G-4274-P0-AX AJ0-7229C5 — — — 00G-4043-P0-AX —C8(2) — — 00F-4249-P0-AX 00G-4249-P0-AX AJ0-7840C18(2) 00B-4252-P0-AX 00D-4252-P0-AX 00F-4252-P0-AX 00G-4252-P0-AX AJ0-7839CN — — — 00G-4255-P0-AX AJ0-8220Phenyl-Hexyl — 00D-4257-P0-AX 00F-4257-P0-AX 00G-4257-P0-AX AJ0-7841NH2 — — 00F-4378-P0-AX 00G-4378-P0-AX AJ0-8162PFP(2) — 00D-4448-P0-AX 00F-4448-P0-AX 00G-4448-P0-AX AJ0-8377HILIC — 00D-4450-P0-AX 00F-4450-P0-AX 00G-4450-P0-AX AJ0-8829

for ID: 18 - 29 mm

5 μm Axia™ Packed Preparative Columns (mm) (cont'd)SecurityGuard

Cartridges (mm)Phases 50 x 30 100 x 30 250 x 30 15 x 30 ◊

/eaSilica(2) — — 00G-4274-U0-AX AJ0-8312C8(2) — 00D-4249-U0-AX — AJ0-8302C18(2) 00B-4252-U0-AX 00D-4252-U0-AX 00G-4252-U0-AX AJ0-8301Phenyl-Hexyl — — 00G-4257-U0-AX AJ0-8303PFP(2) — 00D-4448-U0-AX — AJ0-8378HILIC — — 00G-4450-U0-AX AJ0-8830

for ID: 30 - 49 mm

* SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-4282‡ SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-9281

** PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223

♦ PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8277

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]

Australiat: +61 (0)2-9428-6444 f: +61 (0)2-9428-6445

[email protected]

Austriat: +43 (0)1-319-1301f: +43 (0)1-319-1300

[email protected]

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[email protected]

Canadat: +1 (800) 543-3681f: +1 (310) 328-7768

[email protected]

Chinat: +86 400-606-8099f: +86 (0)22 2532-1033

[email protected]

Denmarkt: +45 4824 8048f: +45 4810 6265

[email protected]

Finlandt: +358 (0)9 4789 0063f: +45 4810 6265

[email protected]

Francet: +33 (0)1 30 09 21 10 f: +33 (0)1 30 09 21 11

[email protected]

Germanyt: +49 (0)6021-58830-0f: +49 (0)6021-58830-11

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Italyt: +39 051 6327511f: +39 051 6327555

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Mexicot: 01-800-844-5226f: 001-310-328-7768

[email protected]

The Netherlandst: +31 (0)30-2418700 f: +31 (0)30-2383749

[email protected]

New Zealandt: +64 (0)9-4780951f: +64 (0)9-4780952

[email protected]

Norwayt: +47 810 02 005f: +45 4810 6265

[email protected]

Puerto Ricot: +1 (800) 541-HPLCf: +1 (310) 328-7768

[email protected]

Spaint: +34 91-413-8613f: +34 91-413-2290

esp [email protected]

Swedent: +46 (0)8 611 6950f: +45 4810 6265

[email protected]

United Kingdomt: +44 (0)1625-501367f: +44 (0)1625-501796

[email protected]

USAt: +1 (310) 212-0555f: +1 (310) 328-7768

[email protected]

All other countries

Corporate Office USA t: +1 (310) 212-0555f: +1 (310) 328-7768

[email protected]

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

Trademarks Luna, Kinetex, and Strata are registered trademarks and SecurityGuard, Novum, Phenex, Axia, and MidBore are trademarks of Phenomenex. ACE is a registered trademark and Excel is a trademark of Advanced Chromatography Technologies Limited. Waters, ACQUITY, UPLC, and CORTECS are registered trademarks of Waters Corporation. Agilent and ZORBAX are registered trademarks of Agilent Technologies, Inc. Thermo Scientific is a registered trademark and Syncronis is a trademark of Thermo Fisher Scientific. Hypersil GOLD is a registered trademark of Thermo Hypersil-Keystone LLC. AkzoNobel and Kromasil are registered trademarks of AkzoNobel Pulp and Performance Chemicals AB. YMC is a registered trademark of YMC Co., Ltd. API 4000 is a trademark of AB SCIEX Pte. Ltd. AB SCIEX™ is being used under license. Supelco is a registered trademark and Titan is a trademark of Sigma-Aldrich Co. LLC. HALO is a registered trademark of Advanced Materials Technology, Inc. ("AMT").

Disclaimer Phenomenex is not affiliated with Advanced Chromatography Technologies Limited, Agilent Technologies, AkzoNobel, Thermo Fisher Scientific, YMC, Waters Corporation, Sigma-Aldrich, or AMT.


Axia column and packing technology is patented by Phenomenex. U.S. Patent No. 7, 674, 383

Kinetex EVO is patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,038 and foreign counterparts.

SecurityGuard is patented by Phenomenex. U.S. Patent No. 6,162,362 CAUTION: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP or ULTRA holders, or to any cartridges.

Strata-X is patented by Phenomenex. U.S. Patent No. 7,119,145

© 2017 Phenomenex, Inc. All rights reserved.BR

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