World Congress on BIOTECHNOLOGY-2011 Biotechnologyelectron microscopy(SEM),Cyclic voltametry(CV) and...
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BIOTECHNOLOGY-2011 384 21-23 March 2011 World Congress on Biotechnology A enzyme electrode for biosensor application based on layer by layer technique using cholesterol oxidase(COx) as a enzyme catalyst presented here.e technique is based on direct electron transfer between active site of enzyme and carbon nanotube(CNT) modified electron surface.Carbon nanotube mixed with nafion and Polyethyleneimine(PEI) solution is deposited directly on carbon fiber(CF).e cholesterol oxidase is deposited on CF/CNT matrix by entrapment of enzyme with PEI film.e PEI is positively charged acts as a binder between the negatively CF/CNT surface and COx enzyme.en a layer of nafion is deposited on both side of the CF/CNT/PEI/COx surface which is the bioelectrode.e bioelectrode is attached to gold electrode and characterized using scanning electron microscopy(SEM),Cyclic voltametry(CV) and electrochemical Impedance Spectroscopy(EIS). References 1. J.Wang, Carbon nanotube based Electrochemical Biosensor:A Review, Electroanalysis 17,No 1 (2005)pp 7-14. 2. Urmila Saxena, Madhuri Das, Seraj Ahmed, Lepakshi Barbora, Mala Borthakur, Anil Verma, Utpal Bora and Pranab Goswami* Multiwalled Carbon Nanotube-Based Enzyme Electrode for Total Cholesterol Estimation in Human Serum, Journal of Experimental Nanoscience (2010) DOI TJEN-2010- 0078-R1. Fabrication of Cholesterol Oxidase bioelectrode using Carbon nanotube and Polyethyleneimine with Carbon nanaotube based direct electron transfer for Biosensor application Madhuri Das and Pranab Goswami Indian Institute of Technology Guwahati, India doi:10.4172/2155-6210.1000001
Transcript of World Congress on BIOTECHNOLOGY-2011 Biotechnologyelectron microscopy(SEM),Cyclic voltametry(CV) and...
BIOTECHNOLOGY-2011
384
21-2
3 M
arch
2011
World Congress on
Biotechnology
A enzyme electrode for biosensor application based on layer by layer technique using cholesterol
oxidase(COx) as a enzyme catalyst presented here.The technique is based on direct electron transfer between active site of enzyme and carbon nanotube(CNT) modified electron surface.Carbon nanotube mixed with nafion and Polyethyleneimine(PEI) solution is deposited directly on carbon fiber(CF).The cholesterol oxidase is deposited on CF/CNT matrix by entrapment of enzyme with PEI film.The PEI is positively charged acts as a binder between the negatively CF/CNT surface and COx enzyme.Then a layer of nafion is deposited on both side of the CF/CNT/PEI/COx surface which is the bioelectrode.The bioelectrode is attached to
gold electrode and characterized using scanning electron microscopy(SEM),Cyclic voltametry(CV) and electrochemical Impedance Spectroscopy(EIS).
References
1. J.Wang, Carbon nanotube based Electrochemical Biosensor:A Review, Electroanalysis 17,No 1 (2005)pp 7-14.
2. Urmila Saxena, Madhuri Das, Seraj Ahmed, Lepakshi Barbora, Mala Borthakur, Anil Verma, Utpal Bora and Pranab Goswami* Multiwalled Carbon Nanotube-Based Enzyme Electrode for Total Cholesterol Estimation in Human Serum, Journal of Experimental Nanoscience (2010) DOI TJEN-2010-0078-R1.
Fabrication of Cholesterol Oxidase bioelectrode using Carbon nanotube and Polyethyleneimine with Carbon nanaotube based direct electron transfer for Biosensor applicationMadhuri Das and Pranab GoswamiIndian Institute of Technology Guwahati, India
doi:10.4172/2155-6210.1000001
