WORKSHOP ON APPROACHES FOR RISK ASSESSMENT OF … of Abstracts-Workshop on... · 2017-07-04 ·...

BOOK OF ABSTRACTS

WORKSHOP ON APPROACHES FOR RISK ASSESSMENT OF EMERGING

CONTAMINANTS with ROUNDE TABLE „How to use EU funds“

University of Novi Sad, Faculty of Technology Novi Sad,

Novi Sad, Serbia

16-17th November 2015

Title: Book of Abstracts:. WORKSHOP ON APPROACHES

FOR RISK ASSESMENT OF EMERGING CONTAMINANTS with ROUND TABLE ”How to use EU funds”

Published by: University of NoviSad, FACULTY OF TECHNOLOGY,

Bulevar cara Lazara 1, Novi Sad, Serbia For publisher: Prof. Dr. Radomir Malbaša, Dean, Faculty of Technology,

Bulevar cara Lazara 1, Novi Sad,Serbia Editor: Prof. Dr. Biljana Škrbić, University of Novi Sad, Faculty of

Technology, Bulevar cara Lazara 1, Novi Sad, Serbia

No. of copies – 50

SCIENTIFIC COMMITTEE

President

Prof. Dr. Biljana Škrbić, University of Novi Sad, Faculty of Technology Novi Sad, Serbia

Members

Prof. Dr. Kiwao Kadokami, University of Kitakyushu,Faculty of Environmental Engineering, Japan

Prof. Dr. Csaba Vágvölgyi, University of Szeged, Faculty of Science and Informatics, Hungary

Prof. Dr. Mira Petrović, Institut Català de Recerca de l’Aigua, Girona, Spain

Dr. Yaqin Ji, Nankai University, Tianjin, China

Dr. Marinella Farré, CSIC-Institute of Environmental Assessment and Water Research, Spain

Prof. Dr. Maria Joao Fraqueza, Technical University of Lisbon, Portugal

Prof. Dr. Biljana Petanovska-Ilievska, Food Institute, Faculty of Agricultural Sciences and Food, “Ss. Cyril and Methodius” University in Skopje

Prof. Dr. Zlatan Sarić, Agriculture and Food Sciences, University of Sarajevo, Bosnia and Herzegovinia

ORGANIZING COMMITTEE

Prof. Dr. Biljana Škrbić, University of Novi Sad, Faculty of Technology Novi Sad, Serbia

Assoc. Prof. Dr. Jelena Cvejanov, University of Novi Sad, Faculty of Technology Novi Sad, Serbia

Assist. Prof. Dr. Nataša Đurišić-Mladenović, University of Novi Sad, Faculty of Technology Novi Sad, Serbia

Dr. Jelena Živančev, University of Novi Sad, Faculty of Technology Novi Sad, Serbia

Radmila Lukić, University of Novi Sad, Faculty of Technology Novi Sad, Serbia

Forewords The Workshop on approaches for risk assessment of emerging contaminants with round table „How to use EU funds“ organized by the Laboratory for Chemical Contaminants and Sustainable Development and the Center of Excellence in Food Safety and Emerging Risks (CEFSER) of the Faculty of Technology University of Novi Sad is dedicated to the protection of environment and different approaches for health risk assessment of emerging contaminants. The Workshop has attention to gather researchers from different multy and inter-disciplinary scientific areas covering the environmental pollutions and food safety issues. The program of the Workshop is divided in three main pillars relating to: I. Environmental Pollution II. Application of Green Technologies in the Environmental Protection III. Chemical Food Safet, with the aim to present the results of the new gathered knowledge created through realization of the contracts signed with the Provincial Secretariat for Science and Technological Development titled: - Influence of anthropogenic activities on the urban contamination level: occurrence of xenobiotics in street dust and soil as an indicator of the air pollution (No.114-451-1148/2014-2) and - Estimation of chemical safety of market basket and population dietary exposure (No.114-451-771/2015-2); the Serbian Ministry of Education, Science and Development: - Development and application of the advanced chromatographic and spectrometric methods in the analysis of xenobiotics and their degradation pathways in biotic and abiotic matrices (no.172050), and through the German Ministry of the Environment, Climate Protection and the Energy Sector - Influence of the wastewater discharge on the recipient - Preliminary study on sustainable water management in the Danube Basin (Serbia) (No. 0602-127-128). However, to extend the networking with international scientific organizations the round table “How to use EU fund” is organized with the aim to share ideas and experience, organized join scientific activities and to disseminate the knowledge gained through preparation of proposals in the frame of EU open calls for proposals such as IPA, HORISON 2020, COST, Erasmus+, EURECA, INTERREG. Moreover, this Workshop intends to make a forum for exchange of research ideas and results concerning the influence of food and the environment on the health in the light of the EU open call for proposal in 2016-2017.

Hoping that the Workshop will give the additional value to the environmental issues and green solution approaches and strengthen the research capacity of the participating institutions, I wish all of you welcome to Novi Sad and any professional success, Sincerely, In Novi Sad,

November 6, 2015. Prof. Dr. Biljana Škrbić

Contents:

Program of Workshop Presentation on the achievements of the CEFSER Centre and Laboratory for Chemical Contaminants and Sustainable Development, Biljana Škrbić ................................................................................................................................................................ 15 Chemical Food Safety RP-HPLC method development for determination of pesticide residues in apple juice, Lenche Velkoska-Markovska, Biljana Petanovska-Ilievska ................................................................................................................................................................ 17 Overview of the PT schemes on mycotoxins determination: CEFSER achievements, Biljana Škrbić,Jelena Živančev, Igor Antić ................................................................................................................................................................ 18 Occurrence of aflatoxin M1 in different types of milk from Serbia: Exposure assessment, Biljana Škrbić,Jelena Živančev, Igor Antić ................................................................................................................................................................ 19 Levels of aflatoxin M1 in selected Serbian white and hard cheese, Biljana Škrbić, Igor Antić, Jelena Živančev ................................................................................................................................................................ 20 Natural co-occurrence of 11 mycotoxins in wheat and wheat based products in Serbia, Biljana Škrbić, Jelena Živančev, Nataša Đurišić-Mladenović ................................................................................................................................................................ 21 Determination of 11 mycotoxins in rice by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry, Biljana Škrbić, Jelena Živančev, Igor Antić ....................................................................................................................................................................... 22 Comparison of the dry fermented sausages from distinct origins based on the toxic heavy elements analysis, Maria Fraqueza, Biljana Škrbić, Nataša Đurišić-Mladenović ....................................................................................................................................................................... 23 Daily intake of heavy elements by Spanish and Serbian infants through consumption оf different baby food types, Biljana Škrbić, Jelena Živančev, Igor Antić, Grigorije Jovanović, Jelena Cvejanov, Marinella Farre ....................................................................................................................................................................... 24 Heavy elements and phthalate esters in rice collected from the Serbian and Chinese market: Concentrations and risk assessment,Yaqin Ji, Biljana Škrbić, Igor Antić, Jelena Živančev ....................................................................................................................................................................... 25 Application of Green Technologies in the Environmental Protection Degradation of phenol and aniline derivatives by Phanerochaete chrysosporium strains, Csaba Vágvölgyi, Mónika Vörös, Bettina Bóka, Dejana Panković, Mira Pucarević, Ljubinko Jovanović, László Manczinger ................................................................................................................................................................ 27 Survey of the pollution status and microbial community structure of the surface water layer of the Maros River, Lóránt Hatvani, László Manczinger, Andrea Palágyi, Isidora Radulov, Lucian Nita, Lívia Vidács, Csaba Vágvölgyi ................................................................................................................................................................ 28

New methods for monitoring and assessing soil quality Manczinger László, Isidora Radulov, Lucian Nita, Andrea Palágyi, László Kredics, János Varga, Beáta Tóth, Csaba Vágvölgyi ................................................................................................................................................................ 29 Environmental Pollution Latest emerging pollutants in the environment, Mira Petrović, Biljana Škrbić, Marinella Farre ................................................................................................................................................................ 31 An overview on “green” approaches in the targeted analysis, Biljana Škrbić, Nataša Đurišić-Mladenović, Jelena Živančev, Igor Antić, Đorđe Tadić ................................................................................................................................................................ 32 Method for multicompound screening of the environmental samples, Kiwao Kadokami, Biljana Škrbić, Nataša Đurišić-Mladenović, Igor Antić, Mira Čelić, Nevenka Nikolić ................................................................................................................................................................ 33 Simulataneous preparation of the soil samples for the PAH and OCC analyses, Biljana Škrbić, Nataša Đurišić-Mladenović, Igor Antić, Vesna Marinković, Anita Petrović-Gegić ................................................................................................................................................................ 34 Risk assessment based on the analysis of SVOCs in soil and street dust samples from Novi Sad, Biljana Škrbić, Đorđe Tadić, Jelena Cvejanov, Vesna Marinković, Anita Petrović-Gegić ................................................................................................................................................................ 35 Hormones in water, Mira Petrović, Biljana Škrbić,Mira Čelić ................................................................................................................................................................ 36 Perfluorinated compounds in surface and waste water, Biljana Škrbić, Kiwao Kadokami, Igor Antić, Đorđe Tadić, Birgit Schlichtig, Bertram Kuch, Jovana Husemann ................................................................................................................................................................ 38 Distribution of phthalates in soil and street dust samples from Novi Sad and the related risk estimation, Yaqin Ji, Biljana Škrbić, Nataša Đurišić-Mladenović ................................................................................................................................................................ 40 Heavy elements in soil and street dust samples from Novi Sad, Biljana Škrbić,Yaqin Ji, Igor Antić, Maja Buljovčić, Jelena Cvejanov, Vesna Marinković ....................................................................................................................................................................... 41 BTEX in indoor air of screen printing industry, Anita Petrović-Gegić,Vesna Marinković ....................................................................................................................................................................... 43 Publications of the Center of Excellence in Food Safety and Emerging Risks (CEFSER) and Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology ................................................................................................................................................................ 44 Authors' Index ......................................................................................................................................... 58

PROGRAM

WORKSHOP ON APPROACHES FOR RISK ASSESSMENT OF EMERGING CONTAMINANTS

with ROUNDE TABLE „How to use EU funds“

University of Novi Sad, Faculty of Technology Novi Sad,

Novi Sad, Serbia

16-17th November 2015

10

16 November 2015

10.00-10.30 Registration

10.30-10.40 Welcome speech, Prof. Dr. Biljana Škrbić, Head of the Center of Excellence in Food Safety and Emerging Risks (CEFSER) and Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad

10.40-11.10 Presentation on the achievements of the CEFSER Centre and Laboratory for Chemical Contaminants and Sustainable Development, Prof. Dr. Biljana Škrbić

11.10-11.40 Interdisciplinary trends in the environmental sector – A cooperation between the

Department of Microbiology, Faculty of Science and Informatics, University of Szeged, and CEFSER-Faculty of Technology Novi Sad, Prof. Dr. Csaba Vagvolgyi, Head of the Department1, Biljana Škrbić2, Laszlo Manczinger1,

1Faculty of Science and Informatics, University of Szeged, Hungary 2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad

11.40-12.00 Coffee break 12.00-13.00 ROUND TABLE "How to use EU funds?" 13.00-14.30 Lunch break Chemical Food Safety

14.30-14.40 RP-HPLCmethod development for determination of pesticide residues in apple juice, Lenche Velkoska-Markovska, Biljana Petanovska-Ilievska Food Institute, Faculty of Agricultural Sciences and Food, “Ss. Cyril and Methodius”

University in Skopje 14.40-14.50 Overview of the PT schemes on mycotoxin determination: CEFSER achievements,

Biljana Škrbić,Jelena Živančev, Igor Antić Center of Excellence in Food Safety and Emerging Risks (CEFSER), Faculty of

Technology Novi Sad

14.50-15.00 Occurrence of aflatoxin M1 in different types of milk from Serbia: Exposure assessment, Biljana Škrbić, Jelena Živančev, Igor Antić

Center of Excellence in Food Safety and Emerging Risks (CEFSER), Faculty of Technology Novi Sad

15.00-15.10 Levels of aflatoxin M1 in selected Serbian white and hard cheese,Biljana Škrbić, Igor Antić, Jelena Živančev,


11

15.10-15.20 Natural co-occurrence of 11 mycotoxins in wheat and wheat based products in Serbia, Biljana Škrbić, Jelena Živančev, Nataša Đurišić-Mladenović


15.20-15.30 Determination of 11 mycotoxins in rice by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry, Biljana Škrbić, Jelena Živančev, Igor Antić;


15.30-15.50 Coffee break 15.50-16.00 Comparison of the dry fermented sausages from distinct origins based on the toxic

heavy elements analysis, Maria Fraqueza1, Biljana Škrbić2, Nataša Đurišić-Mladenović2 1Technical University of Lisbon, Portugal 2Center of Excellence in Food Safety and Emerging Risks (CEFSER), Faculty of Technology Novi Sad

16.00-16.10 Daily intake of heavy elements by Spanish and Serbian infants through consumption оf different baby food types, Biljana Škrbić1, Jelena Živančev1, Igor Antić1, Grigorije Jovanović2, Jelena Cvejanov1, Marinella Farre3

1Center of Excellence in Food Safety and Emerging Risks (CEFSER), Faculty of Technology Novi Sad

2University of Novi Sad, Faculty of Medicine, Novi Sad 3Department of Environmental Chemistry, Institute of Environmental Assessment and

Water Studies, CSIC, Barcelona, Spain

16.10-16.20 Heavy elements and phthalate esters in rice collected from the Serbian and Chinese market: Concentrations and risk assessment,Yaqin Ji1, Biljana Škrbić2, Igor Antić2, Jelena Živančev2,

1College of Environmental Science and Engineering, Nankai University, China 2Center of Excellence in Food Safety and Emerging Risks (CEFSER), Faculty of

Technology Novi Sad

16.20-16.30 Discussion on the possibilities of regional networking and knowledge transfer 17 November 2015

Application of Green Technologies in the Environmental Protection

10.00-10.10 Degradation of phenol and aniline derivatives by Phanerochaete chrysosporium strains,Csaba Vágvölgyi1, Mónika Vörös1, Bettina Bóka1, Dejana Panković2, Mira Pucarević2, Ljubinko Jovanović2, László Manczinger1 1University of Szeged, Faculty of Science and Informatics, Department of Microbiology, Szeged, Hungary 2University EDUCONS, Sremska Kamenica, Serbia

12

10.10-10.20 Survey of the pollution status and microbial community structure of the surface water layer of the Maros River, Lóránt Hatvani1, László Manczinger1, Andrea Palágyi1, Isidora Radulov2, Lucian Nita2, Lívia Vidács3, Csaba Vágvölgyi1 1University of Szeged, Faculty of Science and Informatics, Department of Microbiology, Szeged, Hungary 2Banat University of Agricultural Sciences and Veterinary Medicine, Timişoara, Romania

3Directorate for Water Management of Lower Tisza District (ATI-VIZIG), Szeged, Hungary

10.20-10.30 New methods for monitoring and assessing soil quality Manczinger László1, Isidora Radulov2, Lucian Nita2, Andrea Palágyi1, László Kredics1,

János Varga1, Beáta Tóth3, Csaba Vágvölgyi1 1University of Szeged, Faculty of Science and Informatics, Department of Microbiology, Szeged, Hungary 2Banat University of Agricultural Sciences and Veterinary Medicine, Timişoara, Romania 3Cereal Research Nonprofit Ltd., Szeged, Hungary

Environmental Pollution

10.30-10.40 Latest emerging pollutants in the environment, Mira Petrović1, Biljana Škrbić2,Marinella Farre3 1Institut Català de Recerca de l’Aigua, Spain 2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad 3CSIC-Institute of Environmental Assessment and Water Research, Spain

10.40-10.50 An overview on “green” approaches in the targeted analysis, Biljana Škrbić, Nataša Đurišić-Mladenović, Jelena Živančev, Igor Antić, Đorđe Tadić

Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad

10.50-11.00 Method for multicompound screening of the environmental samples, Kiwao Kadokami1, Biljana Škrbić2, Nataša Đurišić-Mladenović2, Igor Antić2, Mira Čelić2, Nevenka Nikolić3 1 Faculty of Environmental Engineering, University of Kitakyushu, Japan 2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad 3Public Water Management Company “Vode Vojvodine“ Novi Sad

11.00-11.25 Coffee break

11.25-11.35 Simulataneous preparation of the soil samples for the PAH and OCC analyses,Biljana Škrbić1, Nataša Đurišić-Mladenović1, Igor Antić1, Vesna Marinković2, Anita Petrović-Gegić2

1Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad 2Higher Education Technical School of Professional Studies, Školska 1, 2100 Novi Sad

13

11.35-11.45 Risk assessment based on the analysis of SVOCs in soil and street dust samples from Novi Sad, Biljana Škrbić1, Đorđe Tadić1, Jelena Cvejanov1, Vesna Marinković2, Anita Petrović-Gegić2 1Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad 2Higher Education Technical School of Professional Studies, Školska 1, 2100 Novi Sad

11.45-11.55 Hormones in water,Mira Petrović1, Biljana Škrbić2,Mira Čelić2 1Institut Català de Recerca de l’Aigua, Spain 2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad

11.55-12.05 Perfluorinated compounds in surface and waste water, Biljana Škrbić2, Kiwao Kadokami1, Igor Antić2, Đorđe Tadić2, Birgit Schlichtig3, Bertram Kuch3, Jovana Husemann3, 1 Faculty of Environmental Engineering, University of Kitakyushu, Japan 2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad 3Institute for Sanitary Engineering, Water Quality and Solid Waste Management,University Stuttgart, Stuttgart, Germany

12.05-12.15 Distribution of phthalates in soil and street dust samples from Novi Sad and the related risk estimation, Yaqin Ji1, Biljana Škrbić2, Nataša Đurišić-Mladenović2, 1College of Environmental Science and Engineering, Nankai University, China

2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad,

12.15-12.25 Heavy elements in soil and street dust samples from Novi Sad,Biljana Škrbić1, Yaqin Ji2, Igor Antić1, Maja Buljovčić1, Jelena Cvejanov1, Vesna Marinković3 1Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad 2College of Environmental Science and Engineering, Nankai University, China 3Higher Education Technical School of Professional Studies, Školska 1, 2100 Novi Sad

12:25-12:35 BTEX in indoor air of screen printing industry, Anita Petrović, Vesna Marinković

Higher Education Technical School of Professional Studies, Novi Sad, Serbia

12.35-14.10 Lunch break

14.30-16.30 Demonstration of the developed analytical methods in the CEFSER Lab

14

ABSTRACTS

15

Presentation on the achievements of the CEFSER Centre and Laboratory for Chemical Contaminants and Sustainable Development

Prof. Dr. Biljana Škrbić



CEFSER is Centre of Excellence in Food Safety and Emerging Risks Laboratory established during FP7 project CEFSER, 2009-2012. It is a unique Western Balkan laboratory with highly sophisticated analytical instruments, collaborating with advanced institutions from EU, China and Japan. In the Centre, the analytical methods for determination of mycotoxins, pesticides, organochlorine pesticides (OCP), polychlorinated biphenyls, pyrethroids, heavy elements, polycyclic aromatic hydrocarbons and other contaminants in foodsamples are developed and some of them (mycotoxins, OCP and heavy elements in food) are accredited according ISO 17025, being a part of Laboratory for Food Products Analysis at the Faculty of Technology. The CEFSER has been involved in the numerous proficiency testing schemes proving the efficiency of the developed methods. Apart of the food chemical safety interest of the CEFSER team members, their intensive research activities conducted within various national and international projects dedicated to the environmental pollution and protection, and sustainable approaches in domain of fuels have led to the recent creation of Laboratory for Chemical Contaminants and Sustainable Development recognized by the Faculty’s Statute as an institutional unit. Among the most important achievements of this Laboratory so far are the first results ever in Serbia on the presence of: a) pharmaceuticals in various types of water, b) perfluorinated compounds in food and surface and waste water, c) phthalates in rice from the European and Chines markets, surface soil and street dust samples from Novi Sad, and d) more than 1000 micropollutants in surface waters of Vojvodina. All these achievements are described in more details in some of the following presentations. Acknowledgement.The results presented here are obtained within the FP7-REGPOT-2008-1(CEFSER) project No. 229629 supported under theEuropean Union's Seventh Framework Programmeand EU IPA LACREMED project (HUSRB/1002/214/147)coordinated by Prof. Dr. Biljana Škrbić.

16

Chemical Food Safety

17

RP-HPLCmethod development for determination of pesticide residues in apple juice

Lenche Velkoska-Markovska, Biljana Petanovska-Ilievska

Food Institute, Faculty of Agricultural Sciences and Food, “Ss. Cyril and Methodius” University in Skopje

A reversed-phase high-performance liquid chromatography (RP-HPLC) method for qualitative and quantitative determination of 2,4-D ((2,4-dichlorophenoxy)acetic acid), atrazine, malathion, fenitrothion and parathion residues in apple juices has been developed. The analysis was performed on a LiChrospher 60 RP-select B (250 mm x4 mm, 5 µm, Merck) analytical column with mobile phase consisted of acetonitrile/water (60/40, V/V), flow rate of 1 mL/min, constant column temperature at 25 oC and UV-detection at 220 nm and 270 nm. A solid-phase extraction (SPE) was used for concentration and clean-up of analytes. Specificity, selectivity, linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were testedfor the method validation.The developed method proved satisfactoryvalues of multiple correlation coefficients for calibration curves,excellentprecisionand acceptablevaluesfor recovery of all pesticides. This method was applied for determination of above mentioned pesticide residues in apple juices which were taken from Macedonian markets.The run time of assay obtained from this chromatography condition was about 10 min.

18

Overview of the PT schemes on mycotoxins and heavy elements determination: CEFSER achievements

Biljana Škrbić, Jelena Živančev, Igor Antić

University of Novi Sad, Faculty of Technology Novi Sad, Center of Excellence in Food Safety and Emerging Risks (CEFSER), Serbia

The participation in PT schemes is considered as a powerful tool for detecting and removing sources of common errors due to the lack of quality control (QC) within a laboratory. Therefore PT schemes are welcome because they provide a clear, straightforward way of evaluating the accuracy (trueness and precision) of results obtained each laboratory participating in PTs. Proficiency testing performance of CEFSER accredited laboratoryin the period of 2010to 2015 was reported herein,thanks to the CEFSER lab participation in interlaboratory comparison ofmycotoxins in different matricesand heavy elements in chocolate organizedby the Institute for Reference Materials and Measurements (IRMM), Joint Research Centre, EuropeanCommission and the European Union Reference Laboratory (EU-RL), Rikilt, Wageningen. The aim of the proficiency testing schemeshas been evaluationofperformance of the laboratories analysing mycotoxins and heavy elements in different food items.The assessment of the proficiency was based on the z-score approach. CEFSER lab was participated in the following PT schemes for determination of:

ochratoxin A in wheat and green coffee;

deoxynivalenol, ochratoxin A and zearalenone in millet;

aflatoxin B1 and deoxynivalenol in corn;

aflatoxin M1 in milk;

zearalenone in wheat flour;

aflatoxin B1, ochratoxin A, deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, fumonisins B1 and B2 in feed and

heavy elements in chocolate. Only milk samples were prepared with immunoaffinity columns, while in all other cases mycotoxins method is based on a single extraction step i.e. crude extract followed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS).All the obtained results of CEFSER lab are considered satisfactory, based on z-score values, being always below |z-score| ≤ 2. Acknowledgement.The results presented here are obtained withinthe project No.114-451-771/2015-2 “Estimation of chemical safety of market basket and population dietary exposure” funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina and the project No. 172050 supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme.

19

Occurrence of aflatoxin M1 in different types of milk from Serbia: Exposure assessment



Milk is considered to be a perfect natural food for consumers of all age groups due to its high nutritional value. It is high in protein and a valuable source of calcium, vitamins and antioxidants. However, milk has the greatest potential demonstrated for introducing aflatoxina M1 (AFM1) into to human diet. The frequency of occurrence of AFM1 in commercially available milk and dairy products, the high intake of these products by human population, especially by infant and young children and its probable carcinogenic effect, led to an increased concern about the establishment of measures to control AFM1 contamination. In the light of this concern, the occurrence of AFM1in milk marketed in Serbia and the evaluation of the exposure degree to thetoxin through its consumption by an average citizen was studied. Estimation of the correspondingconcentration of AFB1in feedstuffs was also aimed. The level of AFM1 in 50 samples milk collected from February to June 2013 from Serbian market was determined using simple non-specific sample preparation method based on solid phase extraction (Oasis HLB, Waters) and ultra-high performance liquid chromatography with heated electrospray ionization triple quadrupole mass spectrometry (UHPLC/HESI-MS/MS). The contamination levels of the AFM1 toxin in the analyzed milk samples ranged from < LOD to 1.44 µg/kgwith mean value of 0.30 µg/kg. Thirty-eight (76%) samples were contaminated with AFM1 toxin with levels above 0.05 µg/kgset as maximum allowable concentration by EU. The highest level of 1.44 µg/kgwas found in raw sample of domestically produced milk. Additionally,the estimated intakes of AFM1 for the general Serbian adults were 1.420, 0.769 and 0.503 ng/kg bw/day during February, April and May, respectively, through consumed milk. Based on the AFM1 contamination of milk, the calculated average concentration of aflatoksina B1 (AFB1) in feeds consumed by the producing cows was 18.75 µg/kg. Acknowledgement.The results presented here are obtained within the projectNo. 114-451-771/2015-2 “Estimation of chemical safety of market basket and population dietary exposure” funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme, coordinated by Prof. Dr. Biljana Škrbić.

20

Levels of aflatoxin M1 in selected Serbian white and hard cheese

Biljana Škrbić, Igor Antić, Jelena Živančev


Aflatoxin M1 (AFM1) is an important mycotoxin frequently found in milk and dairy products. AFM1 is amajor metabolic product of aflatoxin B1 and is usually excreted in the milk and urine of dairy cattle that have consumed aflatoxin-contaminated feed. Considering its significant impact on human health, determinationof AFM1 levels in dairy products becomes imperative. The present study was undertaken to investigate, for the first time, the level of AFM1 in 54 samples of white and hard type of cheese produced in Serbia.Two methodologies were developed for sample extraction. The samples of white cheese were extracted with mixture of acetonitrile/water (86:14, v/v) without clean-up step, while in the case of hard cheese, method is based on extraction with dichloromethane/acetonefollowed by solid-phase extraction (SPE) clean-up by SPE C18 cartridges. The analysis of AFM1 in the obtained extracts was performed by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS).Average recoveries for AFM1 were 111% (at 0.25 µg/kg) and 73% (at 0.50 µg/kg) with corresponding precision (RSD) of 9 and 7% for white cheese. In the case of hard cheese, average recovery values from samples spiked at levels of 0.125-0.50 µg/kg were 71-80 %, with RSD ranged from 4 to 10%. The limit of quantification for AFM1 was 0.125 for white and 0.02 µg/kg hard cheeses, depending on type of cheese.Of the 54 analyzed samples, 13% were found to be contaminated with AFM1 above the maximum acceptable level of 0.25 µg/kg set up for AFM1 in some European countries like Switzerland, France, Austria and Italy. This is the first report of occurrence of the studied AFM1 in samples of white and hard cheese from Serbia. Acknowledgement.The results presented here are obtained within the project No. 114-451-771/2015-2 “Estimation of chemical safety of market basket and population dietary exposure” funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme, coordinated by Prof. Dr. Biljana Škrbić.

21

Natural co-occurrence of 11 mycotoxins in wheat and wheat based products in Serbia

Biljana Škrbić, Jelena Živančev, Jelena Cvejanov

University of Novi Sad, Faculty of Technology Novi Sad, Center of Excellence in Food Safety and

Emerging Risks (CEFSER), Serbia A multi-mycotoxin analysis method based on liquid chromatography coupled to triple quadrupole mass spectrometry was developed and applied for the determination of eleven mycotoxins, including aflatoxins B1 (AFB1), G1 (AFG1), B2 (AFB2) and G2 (AFG2), ochratoxin A (OTA), zearalenone (ZON),deoxynivalenol (DON) HT-2 toxin, T-2 toxin and fumonisins B1 (FB1) and B2 (FB2) inwheat flour bought in super markets in Novi Sad, the capitol of the northern Serbian province of Vojvodina. Mycotoxins were extracted from samples by simple one-step solvent extraction without any cleanup.The most prevalent mycotoxin was DON followed by ZON and T-2, while other mycotoxins were not detected in any of the samples. The average value obtained for DON, ZON and T-2 in analyzed samples were 325, 4.6 and 4.1 µg/kg, respectively. All the samples complied with current European/Serbian legislation, except one sample that exceeded

the DON maximum level of 750 g/kg. Additionally, the obtained data were used to estimate the potential exposure levels. The mycotoxin intakes were calculated for average consumers among adults and children and then compared withthe tolerable daily intakes (TDIs).The estimated daily intakes ofZON and T-2 toxins werelower than respective TDIs. However, the dietary exposure to DON was assessed to be higher than TDI, i.e. 1.5 and 1.7 μg/kg bw/day for adults and children, respectively. This presentation gives an important contribution in terms of mycotoxin analysis and occurrence in wheat flour and in assessing the mycotoxicological risk posed by regulated and non-regulated mycotoxins for the final consumers of wheat based products. Acknowledgement.The data presented here are obtained within the project No. 114-451-771/2015-2 “Estimation of chemical safety of market basket and population dietary exposure” funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina, coordinated by Prof. Dr. Biljana Škrbić.

22

Determination of 11 mycotoxins in rice by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry


University of Novi Sad, Faculty of Technology Novi Sad, Center of Excellence in Food Safety and

Emerging Risks (CEFSER), Serbia A rapid, reliable and sensitive method was developed to determine 11 mycotoxins (deoxynivalenol, aflatoxins B1, B2, G1, G2, fumonisins B1 and B2, ochratoxin A, HT-2 and T-2 toxin and zearalenone) simultaneously in rice samples. The method is based on a single extraction step using acetonitrile/water/acetic acid mixture (79/20/1 v/v/v) followed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS). Raw extracts were diluted 1+1 and were injected without any clean-up thank to the selectivity of the MS/MS detectionallowed the elimination of further clean up steps.The fast high UHPLC resolution enables separation ofeleven mycotoxins in only 4 min applying by gradient elution. Quantification of samples was carried out with matrix-matched standards calibration. Method performance characteristics were determined after spiking blank samples on two levels in triplicate. The validation showed that the method performance was good for quantitative work, recoveries mostly between 60% and 115%, and repeatability being from 1.5% to 20% within day and from 6% to 27%between days (as relative standard deviation).Limits of detection and limits of quantification were always below the tolerance levels of mycotoxins set by EuropeanUnion in the matrices evaluated.After being validated, the method has been successfully applied to 15 rice samples collected from Chinese and Serbian market. Acknowledgement.The data presented here are obtained within the project No. 114-451-771/2015-2 “Estimation of chemical safety of market basket and population dietary exposure” funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina, coordinated by Prof. Dr. Biljana Škrbić.

23

Comparison of the dry fermented sausages from distinct origins based on the toxic heavy elements analysis

Maria Fraqueza1, Biljana Škrbić2, Nataša Đurišić-Mladenović2

1Technical University of Lisbon, Portugal

2Center of Excellence in Food Safety and Emerging Risks (CEFSER), Faculty of Technology Novi Sad

Meat and meat based products can be a potential source of inorganic contaminants such as heavy elements some of which are highly toxic. The level of heavy elements in meat and meat products depends on factors such as environmental conditions, type of pasture, genetic characteristic of organisms and technological treatments. The aim of this work is to determine levels of selected heavy elements in representative samples of commercially produced dried sausages from Serbia and Portugal. The elements were determined using graphite furnace atomic absorption spectrometry (GF-AAS) after microwave digestion of samples. The highest concentration level was obtained for iron (Fe) in both types of investigated sausages. Among the toxic elements, lead (Pb) was detected with highest level in analyzed samples.Portuguese dried sausages contained significantly higher concentration of Fe (20.02-329.05 mg/kg) than the Serbian ones (25.79-72.79 mg/kg). On the other hand, in Serbian dried sausages higher levels of Mn (0.70-4.30 mg/kg) were detected in comparison with content of Mn (< LOD-1.88 mg/kg) in Portuguese dried sausages. Content of Cr was higher in Portuguese traditional dried sausages (<0.02-0.15 mg/kg) than in analyzed Serbian traditional dried sausages (<0.02-0.05 mg/kg). Co was detected inonly one Portuguese sample at level of LOD, while in all other cases it was below LOD. The content of Ni was similar in Serbian and Portuguese investigated sausages. Levels of Pb in investigated samples were similar, except for one Serbian sample where the detected concentration was significantly higher (0.34 mg/kg). In most of investigated Portuguese sausages the content of As was below limit of detection, while in the selected Serbian samples found levels of As were from < LOD to 0.1 mg/kg. Regarding the Cd, similar range of concentrations were found in Portuguese sausages (< LOD-0.01 mg/kg), and in Serbian sausage (< LOD-0.03 mg/kg) The heavy element contents of sausages differ probably due to variations in raw materials and products’ formulation as a consequence of natural mineral occurrence. The obtained results contribute to a better chemical characterization of different dry fermented sausages, establishing a heavy element profiles in order to transmit quality confidence and reliability on meat products. Acknowledgement. The data presented here are obtained within the project No. 114-451-771/2015-2 “Estimation of chemical safety of market basket and population dietary exposure” funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina, coordinated by Prof. Dr. Biljana Škrbić.

24

Daily intake of heavy elements by Spanish and Serbian infants through consumption оf different baby food types

Biljana Škrbić1, Jelena Živančev1, Igor Antić1, Grigorije Jovanović2, Jelena

Cvejanov1, Marinella Farre3

1University of Novi Sad, Faculty of Technology Novi Sad , Center of Excellence in Food Safety and Emerging Risks (CEFSER), Serbia

2University of Novi Sad, Faculty of Medicine, Novi Sad

3Department of Environmental Chemistry, Institute of Environmental Assessment and Water Studies, CSIC, Barcelona, Spain

Toxic elementscan enter the food chain through the environment and/or as a consequence of the manufacturing processes making foodstuffs the main human exposure route to these chemical elements. The risk associated with this exposure is of such a big concern for human health that the European Food Safety Agency recommends to reduce the exposure to toxic elements so as to protect especially vulnerable subgroups of population (e.g., infants). Thus, in this study toxic (arsenic (As), cadmium (Cd), lead (Pb), tin (Sn), nickel (Ni)) and essential (cobalt (Co), copper (Cu), chromium (Cr), manganese (Mn) and iron (Fe)) elements were determined in most consumed baby foods (i.e. infant and follow-on formula, vegetables and meat porridges, fruit porridges, etc)collected from Serbian and Spanish market.Concentrations of the analyzed elements were determined by atomic absorption spectrometry with a graphite furnace (AASGF) after microwave digestion of the analysed samples. Concentrations of the essential and potentially toxic elements in 62 samples of various baby food itemswere measured, primarily to assess whether the intakes comply with recommended desired levels for essential and permissible levels for toxic elements.Intakes of Fe and Mn through consumption of infant and follow-on formulas were assessed to be higher than recommended/adequate daily intakes. Only exposure to As and Pb appeared to be of health concern depending on the choice of porridge type. Estimated daily intake of As exceeded the safety limit of3 µg/kg bw/day only for one Spanish porridge made of vegetables and fish, while average daily intake of As (1.44 µg/kg bw/day) was below the corresponding safety limit for the mentioned porridge type.Average daily intakes of Pb for the Spanish porridgesmade of vegetables/fish and vegetables/chicken were 0.09 and 1.04 µg/kg bw/day, respectively, being very closely or above to the respective toxicological value.In regard to the estimated infant intakes of the other toxic (Sn and Ni) and the essential (Co, Cu, Cr, Feand, Mn) elements the values were lower than the respective toxicological values. Acknowledgement. The results presented here are obtained within the project No. 114-451-771/2015-2 "Estimation of chemical safety of market basket and population dietary exposure" supported by Secretariat for Science and Technological Development of the Province of Vojvodina and the project No.172050 funded by the Ministry of Education and Science of the Republic of Serbiaand bilateral project within Programme of scientific and technological cooperation between the Republic of Serbia and the Kingdom of Spain supported by the Ministry of Education and Science of the Republic of Serbia.

25

Heavy elements and phthalate esters in rice collected from the Serbian and Chinese market: Concentrations and risk assessment

Ji Yaqin1, Biljana Škrbić2, Igor Antić2, Jelena Živančev2

1College of Environmental Science and Engineering, Nankai University, China 2University of Novi Sad, Faculty of Technology Novi Sad , Center of Excellence in Food Safety and

Emerging Risks (CEFSER), Serbia

Dietary exposure to phthalate esters(PAEs) and heavy metals is a matter of concern for human health risk through the consumption of rice, vegetables and other major foodstuffs. Thus, this investigation was conducted to survey the levels often heavy elements – arsenic (As), lead (Pb), cadmium (Cd), nickel (Ni), cobalt (Co), chromium (Cr), manganese (Mn), iron (Fe), copper (Cu), tin (Sn) and six PEs – dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), benzylbutyl phthalate (BBP), di(2-ethyl-hexyl) phthalate (DEHP), di-n-octyl phthalate (DOP)in 14 commercial rice samples collected from the Serbian and Chinese market in 2014. For the determination of PAEs, suitable extraction technique with organic solvent is used, while for heavy elements, microwave digestion of samples is applied. The content of PAEs and heavy elements is determined by gas chromatography with mass spectrometry and atomic absorption spectrometry with a graphite furnace, respectively. The mean values of the detected essential elements for both Serbian and Chinese rice samples were as follows: 22.9 and 17.5 mg/kg for Mn, 4.9 and2.0 mg/kg for Fe and 1.8 and 1.5 mg/kg Cu, respectively. Of all analyzed toxic elements only Ni (0.10 mg/kg) was detected in rice samples available on the Serbian market, while As (0.05 mg/kg), Pb (0.16 mg/kg) and Ni (0.05 mg/kg) were found in Chinese rice samples.DEHP was the predominant PAE for all analyzed samples followed by DMP and DBP. Content of PAEs in Serbian samples was in range from 3.4 to 620.8 µg/kg, while in Chinese samples it was in range from 2.9 to 391.8 µg/kg. Additionally,the potential health risk was estimated for general Serbian and Chinese population through the daily consumption of the rice.The estimated intakesof PAEs and heavy elements were fairly below corresponding reference dietary values. Acknowledgement.The results presented here are obtained within the project No. 114-451-771/2015-2 "Estimation of chemical safety of market basket and population dietary exposure" supported by Secretariat for Science and Technological Development of the Province of Vojvodina and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme, coordinated by Prof. Dr. Biljana Škrbić.

26

Application of Green Technologies in

the Environmental Protection

27

Degradation of phenol and aniline derivatives by

Phanerochaete chrysosporium strains

Csaba Vágvölgyi1, Mónika Vörös1, Bettina Bóka1, Dejana Panković2, Mira Pucarević2, Ljubinko Jovanović2, László Manczinger1

1University of Szeged, Faculty of Science and Informatics, Department of Microbiology, Szeged, Hungary

2University EDUCONS, Sremska Kamenica, Serbia

Phanerochaete chrysosporium, a basidiomycetous filamentous fungus, has outstanding pollutant-degrading capabilities with its extracellular and intracellular oxidoreductases. It is a white rot fungus which has a highly efficient lignin degrading enzyme system. With these enzyme systems the fungus can also break down different organic pollutants. In these types of degradation processes, the lignin peroxidase and the manganese peroxidase have great significance. We worked out a new selective medium, containing rose bengal, dichloran and carbendazim, with which the isolation of Phanaerochete strains from soil and from other habitats could be simply accomplished. By this new method P. chrysosporium strains were isolated from different Hungarian habitats. In soil microcosm experiments the investigated Phanerochaete strains were able to degrade various aniline-type herbicides in different soil types. These isolates secreted the lignin degrading enzymes at high level. One of the isolates investigated (Pha 78, SZMC 20961), proved to be promising in remediation processes as its enzyme system breaks down broad range of xenobiotics. In our experiments, this strain degraded different phenol and aniline derivatives both in lignin peroxidase and manganese peroxidase media. The key enzyme in these degradation processes was the manganese peroxidase, as the degradation process was very efficient in manganese peroxidase inductive medium. Acknowledgement.This work was supported by the European Union through the Hungary-Serbia IPA Cross-border Co-operation Programme (PHANETRI, HUSRB/1002/214/068) and by the European Union co-funded by the European Social Fund (TÁMOP-4.2.2/B-10/1-2010-0012).

28

Survey of the pollution status and microbial community structure of the surface water layer of the Maros River

Lóránt Hatvani1, László Manczinger1, Andrea Palágyi1, Isidora Radulov2, Lucian

Nita2, Lívia Vidács3, Csaba Vágvölgyi1


2Banat University of Agricultural Sciences and Veterinary Medicine, Timişoara, Romania 3Directorate for Water Management of Lower Tisza District (ATI-VIZIG), Szeged, Hungary

In the frame of a Hungary-Romania Cross-Border Co-operation Programme we investigated the quality of the water of the Maros River at both sides of the border at ten sampling places. In addition to the compounds presented on the list of EU Water Framework Directive regarding to the priority pollutants in surface waters, those pollutants were also measured that are problematic in the region in the water of Maros. The microbial diversity and genotoxicity values were also determined in the water samples. The tendencies show that the abundance of the important pollutants and inorganic elements depends on the actual season (spring, summer, autumn, winter) in the water of Maros, within a year were explored. The bacterial and fungal diversity of the river were followed in time. Correlation analyses were performed between the results of microbial fingerprints, mutagenic activity data and water pollutant data. Some interesting correlations were found between the inorganic pollutants, bacterium titers and genotoxicity values. The nitrite content of the samples showed strong positive correlation with the Ames test genotoxicity data. The analysis the water chemical, microbiological data and mutagenic activity data resulted in a complex water quality database. Taking into account of the most important inorganic pollutants, seasonal pollution maps of the river were constructed. Pollution hot spots appeared in spring and autumn, first of all on the Romanian part of the River. Acknowledgement.This work was supported in part by the project MARIVMICCOLL (HURO/1001/129/2.2.2), which is implemented under the Hungary-Romania Cross-Border Co-operation Programme 2007-2013 and is part-financed by the European Union through the European Regional Development Fund, Hungary and Romania. The content of this publication does not necessarily represent the official position of the European Union. The content of this publication does not necessarily represent the official position of the European Union.

29

New methods for monitoring and assessing soil quality

Manczinger László1, Isidora Radulov2, Lucian Nita2, Andrea Palágyi1, László Kredics1, János Varga1, Beáta Tóth3, Csaba Vágvölgyi1


2Banat University of Agricultural Sciences and Veterinary Medicine, Timişoara, Romania

3Cereal Research Nonprofit Ltd., Szeged, Hungary

The agroecosystem is made up of many interacting components with multiple goals.

Soil quality is one important part of sustainable agroecosystem management, beside

the water and air quality. Assessing soil quality may help farmers to identify more

sustainable agricultural practices. The classical soil quality monitoring systems

determine mainly the physical and chemical parameters of the investigated soil. In

recent, more complex approaches of soil quality assessment, soil enzyme activities

and soil microbial community diversity values are also frequently measured.

In the frame of a previous Hungary-Romania Cross-Border Co-operation Programme

we investigated the applicability of these approaches for soil quality evaluation. In the

beginning, some typical soil enzymes (glucosidases, amidases, ureases and

phosphatases) were measured with classical laboratory methods. The broad range of

new chromogenic and fluorogenic enzyme substrates available commercially, allows

the routine measurement of many other important enzyme systems connected to

presence of soil microbial communities. Application of microplate photometers and

fluorimeters provides convenient tools for high-throughput sample processing. For the

determination of soil microbial diversity various new molecular methods (DGGE,

TGGE, TTGE, SSCP, t-RFLP, RISA) have been developed in the last twenty years

and applied successfully in the soil quality assessment systems.

Acknowledgment.The project was co-financed by the European Union through the Hungary-Romania

Cross-Border Co-operation Programme 2007-2013 (SOILMAP, HURO/0901/058/2.2.2).

30

Environmental Pollution

31

Latest emerging pollutants in the environment

Mira Petrović1, Biljana Škrbić2, Marinella Farre3

1Institut Català de Recerca de l’Aigua, Spain

2Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad

3CSIC-Institute of Environmental Assessment and Water Research, Spain

A significant number of emerging pollutants resulting from point and diffuse pollution is present in the environment. These pollutants may be mobile and persistent in air, water, soil, sediments and ecological receptors even at low concentrations. They are not commonly monitored but have the potential to enter the environment and cause adverse ecological and human health effects. Although great advances have been made in the detection and analysis of trace pollutants during recent decades, due to the continued development and refinement of specific techniques, a wide array of undetected contaminants of emerging environmental concern need to be identified and quantified in various environmental components. Robust data on their fate and behaviour in the environment, as well as on threats to ecological and human health, are still lacking. Moreover, the ecotoxicological significance of some emerging micropollutants remains largely unknown, because satisfactory data to determine their risk often do not exist. This presentation gives an overview of the pharmaceuticals, perfluorinated compounds and carbon-based nanomaterials as the latest emerging pollutants, their distribution in various environmental compartments and the international efforts to determine the background levels. Acknowledgement.The results presented here are obtained within FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme and the project No. 172050 supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, coordinated by Prof. Dr. Biljana Škrbić.

32

An overview on “green” approaches in the targeted analysis

Biljana Škrbić, Nataša Đurišić-Mladenović, Jelena Živančev, Igor Antić, Đorđe Tadić


Environmental friendly practices have been introduced in analytical chemistry including

targeted analysis of micropollutants due to concern about environment pollution. The

main objectives of “green” aaproaches in targeted analysis are to obtain new analytical

protocols and/or technologies or to modify an old method to incorporate procedures

that use less hazardous chemicals or use smaller amounts of hazardous chemicals.

This concept has been gaining interest particularly in the past decade with a great effort

of development made to obtain analytical technologies able to do direct analysis, using

miniaturized equipment, reduced amounts of solvents, and reducing wastes.

Sample pre-treatment can be considered the most polluting step of the whole analytical

process because it deals with the crude sample, in which the analytes may exist in

1000-fold smaller quantities than bulk constituents, so, often, the use of organic

solvents is required to enrich the target compounds selectively and remove potentially

interfering matter. An ideal “green” protocol avoids sample pre-treatment. However, in

the majority of the cases, this is not feasible, so different strategies have been

developed to obtain greener approaches.

This presentation gives an overview of the efforts made in the Laboratory for Chemical

Contaminants and Sustainable Development to introduce “green” approaches into the

sample preparation methods for analysis of selected micropollutants. It describes the

preconditions, advantageous and disadvantages of protocols like crude extracts for

mycotoxins analysis, and “in-cell” pressurized liquid extraction for semi-volatile organic

compounds.

Acknowledgement. The data presented here were obtained within the project No. 114-451-1148/2014-2 “Influence of anthropogenic activities on the urban contamination level: occurrence of xenobiotics in street dust and soil as an indicator of the air pollution”funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme, coordinated by Prof. Dr. Biljana Škrbić.

33

Method for multicompound screening of the environmental samples

Kiwao Kadokami1, Biljana Škrbić2, Nataša Đurišić-Mladenović2, Igor Antić2, Mira Čelić2, Nevenka Nikolić3

1 Faculty of Environmental Engineering, University of Kitakyushu, Japan


3Public Water Management Company “Vode Vojvodine“ Novi Sad

Chemical substances are indispensable materials for modern society. The number and production volume of them have been rapidly increasing. Thus, it is of utmost importance to have possibility to screen as many as possible chemicals in the environmental matrices, particularly in the aquatic environment, in order to better understand water quality and pollution sources and to allow the implementation of sustainable water use management strategies.However, the number of chemicals and survey frequency are insufficient due to limitation of the existing analytical methods, indicating needs of novel comprehensive analytical methods.

At present two types of analytical instruments, GC-MS and LC-MS, are usually used for analysing chemical substances due to high sensitivity and selectivity. Two novel automated identification and quantification system (AIQSs) developed for them, were used in this study for screening of organic micropollutants in river samples from the Vojvodina

Province, Serbia. AIQS for GC-MS consists of retention times, mass spectra and calibration curves of nearly 1000 semi-volatile organic compounds (SVOCs). In AIQS for LC-TOF-MS, retention times, accurate-mass spectra and calibration curves of 300 polar substances are registered. Thus, this is the first comprehensive study on the occurrence of about 1300 organic micro-pollutants in various water systems throughout the Vojvodina Province, allowing the insight into a complete pollution picture of micro-pollutants in waters of this northern Serbian province. Results revealed

generally low presence of industrial and domestic chemicals in the sediment samples, where dominated micro-pollutants reflecting natural sources and/or faecal contamination, while in the surface water samples prevailed domestic and industrial chemicals.

Acknowledgement.The presented results are obtained with in Japanese Society for the Promotion of Science Fellow Program 2014 and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programme, coordinated by Prof. Dr. Biljana Škrbić.

34

Simultaneous preparation of the soil samples for the PAH and OCC analyses

Biljana Škrbić1, Nataša Đurišić-Mladenović1, Igor Antić1, Vesna Marinković2, Anita Petrović-Gegić2


2Higher Education Technical School of Professional Studies, Školska 1, 2100 Novi Sad

The objectives of this study were to investigate the levels, patterns, spatial variation of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in Novi Sad, Serbia. Surface soil samples were collected during winter 2015. at 15 different locations covering roadsides, schools, recreational, residential and industrial zone. In this study a pressurized liquid extraction method (PLE) so called in-cell, was used for the simultaneous extraction and clean-up of PAHs, PCBs and OCPs in soil samples. The accuracy measured as the efficiencies of the fortified samples ranged from 33.09% to 103.07% for PAHs, 106.37 to 132.80% for PCBs and 73 to 130% for OCPs, respectively.The results showed that the method limit of quantification were 0.003-0.155 ng/g for PAHs, 0.009-0.054 ng/g for PCBs and 0.009-0.134 ng/g (dry weight) for OCPs. The total concentrations of 20 priority PAHs from both USA 16EPA and 15+1 EU lists varied from 22.44 to 2246.64 ng/g dw (mean value 360 ng/g), for PCBs from <0.009 to 32.62 ng/g dw (mean value 2.94 ng/g) and for OCPs from <0.009 to 182.78 ng/g dw (mean value 16.51 ng/g). Concerning the spatial variation, tendencies for higher concentrations of PAHs and PCBs were observed at sampling location near roadsides, while industrial soils exhibited relatively higher concentration of OCPs. The composition pattern of PAHs in soil was characterized by the high molecular weight 4 to 6 rings PAHs, counting 84% of the total PAHs. While, summary of individual PCBs congeners concentrations shows that Novi Sad soil samples are enriched in penta-, hexa-, and hepta- chlorobiphenyls (CB) (80% in mass). The concentrations and profiles OCPs clearly indicated the dominance of ΣDDTs contaminants with average percent of 96% of total OCPs, respectively. It was found that PAHs, PCBs and OCPs levels in the sampled urban soils of Novi Sad were in the range of he levels found in most European soils and other regions. Acknowledgement. The data presented here were obtained within the project No. 114-451-1148/2014-2 “Influence of anthropogenic activities on the urban contamination level: occurrence of xenobiotics in street dust and soil as an indicator of the air pollution”funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina and the project No. 172050 partly funded by the Ministry of Education, Science and Technological Development of the Republic, coordinated by Prof. Dr. Biljana Škrbić.

35

Risk assessment based on the analysis of SVOCs in soil and street dust samples from Novi Sad

Biljana Škrbić1, Đorđe Tadić1, Jelena Cvejanov1, Vesna Marinković2, Anita Petrović-Gegić2



Organic pollutants are known to accumulate in surface soils as a result of both contaminations from point sources and from long-range aerial transport. Street dust is an indicator of pollution status of a city area, while soil generally acts as a sink throughout the year for medium to low volatility atmospheric PAHs however, during summer soil becomes a source, especially for volatile PAHs. PAHs are receiving extensive attention because of their toxicity, which include probable mutagenic and carcinogenic effects and exert of potential health risk to urban residents. Therefore, a comprehensive investigation was conducted to the urban surface soil and street dust in Novi Sad due to investigate 16 United States Environmental Protection Agency (US EPA) polycyclic aromatic hydrocarbons (PAHs) and selected one from the 15+1 EU PAHs list content in 30 surface soil and street dust samples collected from 15 different urban locations in Novi Sad, Serbia.In order to evaluate the carcinogenic potency of investigated PAHs detected in surface soil, the toxic equivalency factors (TEFs) were used to estimate the toxicity equivalent (BaPeq) concentrations of soil PAHs.The cancer risk (CRs) of studied PAHs were calculated for two age groups (children and adults) over the three exposure pathways: ingestion (CRing), dermal contact (CRderm) and inhalation (Crinh). The Total Lifetime Carcinogenic Risk (TLCR) was calculated by summing the CRs. The proportion of the concentration sum of seven carcinogenic PAHs to the concentration sum of the investigated selected PAHs was calculated, too. The so-called mutagenic equivalent (MEQ) can also be useful as indicator to assess the mutagenic PAH mixture health effect.

The total lifetime carcinogenic risk (TLCR) mean values for surface soil were 5.94x10-

4 and 4.2x10-4 for children and adults, respectively indicating high health risk. While mean TLCR values for street dust samples were 3.03x10-4 and 3.54x10-4 for children and adults, respectively. Mean MEQ and ∑PAHcarc/∑PAH values were 71.98 and 0.45, and 3.5 and 0.28 estimated for surface soil and street dust samples respectively. In general, PAHs in all sampling locations in the studied area of Novi Sad are likely to pose a potential health risk for population of Novi Sad based on ingestion, dermal contact and inhalation exposure pathways and estimated mutagenic equivalent. All estimation is based on lower bond approach using zero value as concentration of every investigated PAHs being lower than the LOD.

Acknowledgement.The data presented here were obtained within the project No. 114-451-1148/2014-2“Influence of anthropogenic activities on the urban contamination level: occurrence of xenobiotics in street dust and soil as an indicator of the air pollution”funded by the Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina, coordinated by Prof. Dr. Biljana Škrbić.

36

Hormones in water

Mira Petrović1, Biljana Škrbić2, Mira Čelić2

1Institut Català de Recerca de l’Aigua, Spain


Endocrine-disrupting compounds (EDC) are environmental contaminants that disturb normal function and cause male reproductive dysfunction in humans and wildlife. Among the wide range of substances with endocrine-disrupting properties, natural and synthetic estrogens are of particular interest due to their high estrogenic potency. According to the European Union Water Framework Directive (EU WFD 2013/39/EU) the list of priority substances contains only 45 contaminants and/or contaminants group. The natural and synthetic estrogens are still out of the priority list but two of them (such as 17 alpha-ethinylestradiol (EE2) and 17 beta-estradiol (E2)) have been added to a new 'watch list' of emerging aquatic pollutants. Adding them to the new 'watch list' of substances for Union-wide monitoring, the maximum acceptable method detection limit (LOD) for them was defined. Thus, the present work describes the development of an analytical method for simultaneous determination of natural and synthetic estrogens (estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), diethylstilbestrol (DES)) and their conjugates (estrone-3-sulfate, estriol-3-sulfate, estrone-3-glucuronide, estradiol-17-glucuronide and estriol-16-glucuronide) in water samples, which would enableLOD values set up in Article 8b of Directive 2008/105/EC.The method is based on on-line solid-phase extraction (SPE), using dual column switching system coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI) in the negative mode. It was demonstrated that the proposed method is simple, sensitive and suitable for analysis and monitoring of estrogens at very low levels in water samples. Fast and simple sample preparation was employed, i.e. water samples after filtration were injected directly into autosampler and the analytes were preconcentrated on online SPE column. After cleanup step the analytes were eluted in back flash mode and then separated on a liquid chromatography column. The experimental parameters, such as a sample loading flow rate, cleanup condition and elution time were optimized. In that way, different solvents were tested in order to achieve the optimal conditions for ionization of compounds. Besides that, also the influence of different pH values of samples was studied to obtain satisfactory separation of compounds. It has been shown that, high ionization efficiency of all studied analytes was achieved with pH adjustment directly at sample by adding 1% sodium hydroxide. The applicability of the method was tested on four types of water samples, including underground water, drinking water, river water and wastewater from territory of the Province of Vojvodina. The obtained recoveries for all water types ranged from 64.53%

37

to 107.51% while the overall precision of the analysis was satisfactory with average RSD of triplicate measurements ranged from 3.14% to 18.32%. Applying this technique, the low limits of detection (LOD) for all studied compounds was obtained. LODs ranged from 0.020 to 0.147 ng/l, 0.015 to 0.093 ng/l, 0.021 to 0.079 ng/l, 0.023 to 0.306 ng/l for underground water, drinking water, river water and wastewater, respectively. Obtained values are in accordance with the ones specified in Directive 2008/105/EC. The developed method was used to contribute to the discovery of new knowledge of water quality in the Province of Vojvodina. To the authors' the best knowledge it is the first work describing the occurrence of hormones in different types of Serbian water. Acknowledgment.The work is a part of the project No. 172050 partly funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia and COST Action ES1403 (NEREUS).

38

Perfluorinated compounds in surface and waste water

Biljana Škrbić1, Kiwao Kadokami2, Igor Antić1, Đorđe Tadić1, Birgit Schlichtig3, Bertram Kuch3, Jovana Husemann3

1 Faculty of Environmental Engineering, University of Kitakyushu, Japan

2 Laboratory for Chemical Contaminants and Sustainable Development, Faculty of Technology Novi Sad

3Institute for Sanitary Engineering, Water Quality and Solid Waste Management, University Stuttgart, Stuttgart, Germany

A wide range of so-called “emerging” or “new” unregulated contaminants have appeared as an environmental problem and become a hot topic for environmental analytical chemists. Perfluoroalkyl acids (PFAAs) are listed among emerging contaminants because they are globally distributed, environmentally persisted, bioaccumulative and potentially harmful. The PFAAs can be grouped in perfluoroalkyl carboxylic acids (PFCAs), perfluoroalkyl sulfonic acids (PFASs), perfluoroalkyl sulfonamides (PFASA). Although the occurrence of PFAAs in various environmental matrices has been vigorously investigated in the developed countries in the world, to the best of our knowledge there is no data available on PFAAs occurrence in any surface and wastewater of the Vojvodina Province, the Serbian northern part. The method developed in this study is capable to detect 11 PFAAs: perfluorobutane sulfonic acid (PFBS); perfluorooctane sulfonic acid (PFOS); perfluorooctane sulfonamide (PFOSA); perfluorobutyric acid (PFBA); perfluorohexanoic acid (PEHxA); perfluoroheptanoic acid (PFHpA); perfluorooctanoic acid (PFOA); perfluorononanoic acid (PFNA); perfluorodecanoic acid (PFDA); perfluoroundecanoic acid (PFUndA); perfluorododecanoic acid (PFOdA) at low ng/L levels in surface and wastewater samples.

Twelve composite surface samples were collected down and up stream of the wastewater discharged point from the Danube River. Additionally, at the time period of 24 h eight composite wastewater samples were collected directly at discharge point. The water samples were extracted by solid-phase extraction (SPE) with Oasis HLB (225 mg) cartridges and analysed using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) equipped with a PFC-free Thermo Scientific Accela LC system in order to minimize background contamination of analysed samples with perfluorinated compounds. Whole method efficiency at a spiking level of 10 ng/L to 500 mL HPLC grade water was in the range from 64 to 121 %. The relative standard deviations of efficiency ranged from 6 to 14 %, indicating good method precision. Limits of quantification were in accordance with literature published values for PFAAs, in the range from 0.01 to 0.43 ng/L. PFAAs were quantified by internal calibration procedure. Calibration curves for all PFAAs in both studied matrices were linear within the working range from 0.5 ng/L to 30 ng/L. Squared correlation

coefficients (R2) showed good linearity ( 0.99) for all analysed PFAAs. PFOA and PFHxA were the most abundant compounds in surface water samples, while the highest concentration of PFOSA (7.38 ng/L) was detected in wastewater samples. The

39

highest concentration of PFOA and PFHxA in surface water were 11.77 ng/L and 14.24 ng/L, respectively. The obtained results were used in order: to give a preliminary insight into the occurrence of PFAAs in Serbian surface and wastewater samples; to provide the first data of the levels of PFAAs in selected water sample matrices and to compare the determined levels of PFAAs with the ones found in the other countries. Additionally, the occurrence temporal trend is also revealed as the samples were collected in the time period of one month (November-December, 2014).

Acknowledgement.The results presented here are obtained within the project No. 172050 supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia and the project„Influence of the wastewater discharge on the recipient - Preliminary study on sustainable water management in theDanube Basin (Serbia)“ funded by the German Ministry of the Environment, Climate Protection and the Energy Sectorcontract No. 0602-127-128 and FP7-REGPOT-2008-1 (CEFSER) project No. 229629 supported under the European Union's Seventh Framework Programm, coordinated by Prof. Dr. Biljana Škrbić.

40

Distribution of phthalates in soil and street dust samples from Novi Sad and the related risk estimation

Yaqin Ji1, Biljana Škrbić2, Nataša Đurišić-Mladenović2

1College of Environmental Science and Engineering, Nankai University, China


This study reports the presence of 6 phthalic acid esters (PAEs) in 60 composite soil and street dust samples collected in the urban zone of Novi Sad. The results were used to assess for the first time in Serbia the children’s and adults’ PAEs nondietary daily intakes (DIs) through incidental soil and dust ingestion and/or dermal absorption. Levels of six phthalate acid esters: dimethyl phthalate, DMP; diethyl phthalate, DEP; dibutyl phthalate, DBP; butyl benzyl phthalate, BBP; di-(2-ethylhexyl) phthalate, DEHP and dioctyl phthalate, DOP, were determined in the sample extracts by gas chromatograph coupled with mass spectrometer. All six PAEs were detected in every soil and street dust samples in the range 0.0002 – 4.82 mg kg-1. In all soil samples total content of 6 PAEs exceeded the limit sets by the relevant Serbian Regulation for the soil sustainable quality, but were much lower than the limit requiring remediation measures. The results were compared with the relevant literature data: the levels in soils were lower than the urban locations in China, but they seemed to be comparable to the France urban zone. The occurrence of PAEs in the street dust samples was compared with the data reported for Guangzhou city, China: the highest level of DEHP found in the street dust from Novi Sad (4.82 mg kg-1) was 64 times lower than the maximum level of 310.63 mg kg-1 found in in Guangzhou. The estimates of the total DIs of 6 PAEs through nonintentional intake of soil and street dust were much below the known reference values. Children were more susceptible to PAEs intake than the adults regardless of the exposure routes: the total DIs when all 4 exposure pathways were considered (soil ingestion and dermal absorption and street dust ingestion and dermal absorption) was about 4 times higher for children than for adults. All the estimated DIs values were far below the known reference values.

Acknowledgement. The presented results are obtained during the bilateral Chinese-Serbian project “Human exposure assessment to heavy elements, phthalic acid esters and persistent organic pollutants through air, water, dust and food” supported by Secretariat of the Science and Technological Development of the Autonomous Province of Vojvodina contract No. 114-451-3843/2013-01 and project No.172050 partly funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia.

41

Heavy elements in soil and street dust samples from Novi Sad

Biljana Škrbić1, Yaqin Ji2, Igor Antić1, Maja Buljovčić1, Jelena Cvejanov1, Vesna Marinković3


2College of Environmental Science and Engineering, Nankai University, China


The urban environment is affected by a wide range of contaminants, which vary

appreciably within and among cities. Heavy elements are one class of the pollutants

known to accumulate in surface soils and street dust as a result of both contaminations

from point sources and from long-range aerial transport. The propose of this study is

to determine the concentrations and health risk of the soil and street dust for children

and adults taking into account the heavy elements occurrence in surface soil and street

dust samples from urban area of Novi Sad. A total of 30 samples of soil and street

dust were collected from different sites of urban areas in the city of Novi Sad, the

second largest city in Serbia. Concentrations of 10 heavy elements (Pb, Cd, As, Cu,

Cr, Co, Ni, Mn, Fe and Sn) for 30 samples of soil and street dust were analysed. The

samples were microwave digested and the concentrations of heavy elements were

measured by atomic absorption spectrometer (AAS) with a graphite furnace (GF). The

measured concentrations were corrected for recoveries, the range of recoveries, which

were ranged from 72-106%. This is the first report of the heavy elements in street dust.

The average concentration of all studied Pb, Cd, As, Cu, Cr, Co, Ni, Mn, Fe and Sn)

were in the range of 0.11-29800 mg/kg.

Determined concentration were applied to evaluate and to quantify exposure risks

posed to human health with the risk assessment model recommended by the US

Environmental Protection Agency. Exposure of humans to heavy metals in urban soil

and street dust samples can occur via three main pathways: direct oral ingestion of

substrate particles; inhalation of soil particles trough the mouth and nose; and dermal

absorption of heavy metals in particles adhered to exposed skin. Both non-

carcinogenic and carcinogenic risks of these exposure routes were considered. To

assess the overall potential for non-carcinogenic effects posed by all exposure

pathways a Hazard Index (HI) approach has been employed. Children are relatively

vulnerable population in comparison with adults. HI in soil for children is higher than

for adult, being in the ranged between 0.07 to 0.94 and 0.01-0.79, respectively. The

obtained results indicate than HI values for soil are higher than HI values for street

dust.

42

Acknowledgement.The data presented here were obtained within the project No. 114-451-1148/2014-2

“Influence of anthropogenic activities on the urban contamination level: occurrence of xenobiotics in street

dust and soil as an indicator of the air pollution” funded by the Secretariat of the Science and

Technological Development of the Autonomous Province of Vojvodina, coordinated by Prof. Dr. Biljana

Škrbić.

43

BTEX in indoor air of screen printing industry

Anita Petrović, Vesna Marinković

1Higher Education Technical School of Professional Studies, Novi Sad, Serbia

Employees in the printing industry are mostly exposed to the harmful effects of volatile organic compounds (VOCs). The printing industry is using various chemicals (developers, fixers, solvents, inks, wetting agents, varnishes, adhesives, cleaning products), and they all have a negative impact on the health of employees and the environment. The objectives of this study were to investigate the level of BTEX (benzene, toluene, ethylbenzene and xylene) during the process of screen printing in Novi Sad, Serbia. Air samples were taken from standing breathing zone of employees, ~150 cm above the ground level, and were analyzed using gas chromatography with flame ionization detector (GC-FID). The concentrations of benzene varied from <0.0044 to 1.35 mg/m3, toluene from <0.0045 to 0.86 mg/m3 while ethylbenzene and xylene were not detected. Levels of BTEX in indoor air were compared with occupational exposure limit levels. Occupational concentrations of BTEX were within national and international occupational limits throughout the monitoring period, based on 8-hour workday weighted averages. Although the concentrations of BTEX were lower than the national occupational exposure limits, however, most of the printing industry work for 8 h per day and 5 days per week. Under this condition, the exposure risks of employees could not be negligible. Cancer risks were estimated by using the personal exposure concentrations. Lifetime cancer risks for the people working in the screen printing industry were high. Thus, much increase in indoor air exchange rate is required to decrease BTEX concentration to a safe and healthy level. Acknowledgement.The results presented here are obtained within the project “The impact of the graphic and paper industries on the living and working environment” and they are part of the contract No.114-451-1909/2011-01 of Secretariat for Science and Technological Development of the Province of Vojvodina.

44

Publications of the Center of Excellence in Food Safety and Emerging Risks (CEFSER) and Laboratory for Chemical Contaminants and Sustainable

Development, Faculty of Technology

Articles published in journals from the SCI list

1. B. Škrbić, J. Cvejanov, N. Đurišić-Mladenović, Chemometric characterization of vegetable oils based on the fatty acid profiles for selection of potential feedstocks for biodiesel production, J. Biobased Mater. Bioenergy 9 (3), 358-371, 2015, DOI:10.1166/jbmb.2015.1527

2. N. Đurišić-Mladenović, Z. Predojević, B. Škrbić, Conventional and advanced biofuels, Hemijska industrija, DOI:10.2298/HEMIND150311029D

3. B. Škrbić, Z. Predojević,N. Đurišić-Mladenović, Esterification of sludge palm oil as apretreatment step for biodiesel production, Waste Management & Research, 33 (8), 723-729, 2015, DOI: 10.1177/0734242X15587546

4. B. Škrbić, I. Antić, J. Živančev, Presence of aflatoxin M1 in white and hard cheese samples from Serbia, Food Control 50, 111-117, 2015.

5. B. Škrbić, N. Đurišić-Mladenović, N. Mačvanin, A. Tjapkin, S. Škaljac, Polycyclic aromatic

hydrocarbons in smoked dry fermented sausages with protected designation of origin (Petrovská klobása) from Serbia, Maced. J. Chem. Chem. Eng., 33, 227–236, 2014.

6. F. Pérez, M. Llorca, M. Köck-Schulmeyer, B. Škrbić, L. Silva Oliveira, K. da Boit Martinello, N. A.

Al-Dhabi, I. Antić, M. Farré, D. Barceló, Assessment of perfluoroalkyl substances in food items at global scale, Environ. Res., 135, 181–189, 2014.

7. N. Stilinović, B. Škrbić, J. Živančev, N. Mrmoš, N. Pavlović, S. Vukmirović, The level of elements

and antioxidant activity of commercial dietary supplement formulations based on edible mushrooms, Food Funct., 5, 3170-3178, 2014.

8. E. Sajben-Nagy, L. Manczinger, B. Škrbić, J. Živančev, I. Antić, J. Krisch, Cs. Vágvölgyi,

Characterization of an extracellular laccase of Leptosphaerulina chartarum, World J. Microbiol. Biotechnol., 30, 2449-2458, 2014.

9. B. Škrbić, J. Živančev, M. Godula, Multimycotoxin analysis of crude extracts of nuts with ultra-

high performance liquid chromatography/tandem mass spectrometry, J. Food Compos. Anal. 34,

171-177, 2014. 10. B. Škrbić, J. Živančev, I. Antić, M. Godula, Levels of aflatoxin M1 in different types of milk

collected in Serbia: Assessment ofhuman and animal exposure, Food Control, 40, 113-119, 2014. 11. S. Škaljac, Lj. Petrović, T. Tasić, P. Ikonić, M. Jokanović, V. Tomović, N. Džinić, B. Šojić, A.

Tjapkin, B. Škrbić, Influence of smoking in traditional and industrial conditions on polycyclic

45

aromatic hydrocarbons content in dry fermented sausages (Petrovská klobása) from Serbia, Food Control, 40, 12-18, 2014.

12. M. Petrović, B. Škrbić, J. Živančev, L. Ferrando-Climent, D. Barcelo, Determination of 81

pharmaceutical drugs by high performance liquid chromatography coupled to mass spectrometry with hybrid triple quadrupole–linear ion trap in different types of water in Serbia, Sci. Total Environ., 468-469, 415-428, 2014.

13. B. Škrbić, K. Heberger, N. Đurišić-Mladenović, Comparison of multianalyte proficiency test results

by sum of ranking differences, principal component analysis, and hierarchical cluster analysis, Anal. Bioanal. Chem., 405, 8363-8375, 2013.

14. B. Škrbić, J. Živančev, N. Mrmoš, Concentrations of arsenic, cadmium and lead in selected

foodstuffs from Serbian market basket: Estimated intake by the population from the Serbia, Food Chem. Toxicol., 58, 440-448, 2013.

15. B. Škrbić, S. Koprivica, M. Godula, Validation of a method for determination of mycotoxins

subjected to the EU regulations in spices: the UHPLC-HESI-MS/MS analysis of the crude extracts, Food Control, 31, 461-466, 2013.

16. B. Škrbić, N. Đurišić-Mladenović, Distribution of heavy elements in urban and rural surface soils:

the Novi Sad city and the surrounding settlements, Serbia", Environ. Monit. Assess., 185, 457-471, 2013.

17. Cs. Vágvölgyi, E. Sajben –Nagy, B. Boka, M. Voros, A. Berki, A. Palagyi, J. Krisch, B. Škrbić, N.

Đurišić-Mladenović, L. Manczinger, Isoaltion and characterization of antagonistic Bacillus strains capable to degrade ethylenethiourea, Curr. Microbiol., 66, 243-250, 2013.

18. I. Samojlik, V. Mijatović, S. Petković, B. Škrbić, B. Božin, The influence of essential oil of aniseed

(Pimpinella anisum, L.) on drug effects on the central nervous system, Fitoterapia, 83, 1466-1473, 2012.

19. K. Héberger, B. Škrbić, Ranking and similarity for quantitative structure–retention relationship

models in predicting Lee retention indices of polycyclic aromatic hydrocarbons, Anal. Chim. Acta, 716, 92-100, 2012.

20. B. Škrbić,J. Živančev, N. Đurišić-Mladenović, M. Godula, Principal mycotoxins in wheat flour from

the Serbian market: levels and assessment of the exposure by wheat-based products, Food Control, 25, 389-396, 2012.

21. B. Škrbić, S. Milovac, M. Matavulj, Multielement profiles of soil, road dust, tree bark and wood-

rotten fungi collected at various distances from high-frequency road in urban area, Ecol. Ind., 13, 168-177, 2012.

22. Z. Predojević, B. Škrbić, N. Đurišić-Mladenović, Transesterification of linoleic and oleic sunflower

oils to biodiesel using CaO as a solid base catalyst, J. Serb. Chem. Soc., 77, 815-832, 2012.

46

23. B. Škrbić, A. Malachova, J. Živančev, Z. Veprikova, J. Hajšlova, Fusarium mycotoxins in wheat samples harvested in Serbia: A preliminary survey, Food Control, 22, 1261-1267, 2011.

24. Z. Predojević, B. Škrbić, N. Đurišić-Mladenović, Effects of ethanol and MTBE additives on

properties at blends with base gasoline, Fresen. Environ. Bull., 20, 1401-1405, 2011. 25. B. Škrbić, N.Mačvanin, Nutritional and sensorial aspects of wheat bread enriched with high-oleic

sunflower seed, Acta Alimentaria, 40, 194-204, 2011.

26. B. Škrbić, J. Cvejanov, The enrichment of wheat cookies with high-oleic sunflower seed and hull-less barley flour: Impact on nutritional composition, content of heavy elements and physical properties, Food Chemistry, 124, 1416-1422, 2011.

27. T. Stafilov, B. Škrbić, J. Klanova, P. Čupr, I. Holoubek, M. Kočor, N. Đurišić-Mladenović,

Chemometric assessment of the semivolatile organic contaminants content in the atmosphere of the selected sites in the republic of Macedonia, J. Chemometr., 25, 262-274, 2011.

28. S. Žilić, D.Dodig, V. Hadži-Tašković Šukalović, M. Maksimović, G. Saratlić, B. Škrbić, Bread and

durum wheat compared for antioxidants contents, and lipoxygenese and peroxidase activities, Internat. J. Food Sci. Technol., 45, 1360-1367, 2010.

29. B. Škrbić, N. Đurišić-Mladenović, Chemometric interpretation of heavy metal paterns in soils

worldwide, Chemosphere, 80, 1360-1369, 2010. 30. B. Škrbić, N. Ðurišić-Mladenović, N.Mačvanin, Determination of metal contents in sugar beet

(Beta vulgaris) and its products: Empirical and statistical approach, Food Sci. Technol. Res., 16, 123-134, 2010.

31. B. Škrbić, K. Szyrwińska, N. Đurišić-Mladenović, P. Nowicki, J. Lulek, Principal component

analysis of indicator PCB profiles in breast milk from Poland, Environ. Int., 36, 862-872, 2010. 32. M.Šćiban, M.Klašnja, M. Antov, B.Škrbić, Removal of water turbidity by natural coagulants

obtained from chestnut and acorn, Bioresour. Technol., 100, 6639-6643, 2009. 33. Z.Predojević, B. Škrbić, Alkali-catalyzed production of biodiesel from waste frying oils, J. Serb.

Chem. Soc., 74, 993-1007, 2009. 34. B. Škrbić, N. Ðurišić-Mladenović, Levels of PAHs in soil samples from the vicinity of oil refinery

Novi Sad-Serbia, Kuwait J. Sci. Eng., 36 (1A), 63-75, 2009.

35. B. Škrbić, S. Milovac, D. Dodig, B. Filipčev, Effects of hull-less barley flour and flakes on bread nutritional composition and sensory properties, Food Chemistry, 115, 982-988, 2009.

36. B. Škrbić, J.Cvejanov, N. Đurišić-Mladenović, Polycyclic aromatic hydrocarbons in products of a

beet sugar factory in Vojvodina: Levels and intakes, Polycycl. Aromat. Comp., 28, 348-361, 2008.

47

37. B. Škrbić, Z.Predojević, Levels of organochlorine pesticides in crops and related products from Vojvodina, Serbia: Estimated dietary intake, Arch. Environ. Contam. Toxicol., 54, 628-636, 2008.

38. M.Šćiban, M.Klašnja, B.Škrbić, Adsorption of copper ions from water by modified agricultural by-

products, Desalination, 229, 170-180, 2008.

39. B. Škrbić, J. Suđi, R. Vučković, Levels of polycyclic aromatic hydrocarbons in products of the Serbian beet sugar factory, Zuckerindustrie, 133, 31-35, 2008.

40. B. Škrbić, B.Filipčev, Nutritional and sensory evaluation of wheat breads supplemented with oleic-

rich sunflower seed, Food Chemistry, 108, 119-129, 2008.

41. B. Škrbić, Assessment of the Serbian population exposure to polychlorinated biphenyls by crops, Environ. Toxicol. Pharmacol., 25, 171-175, 2008.

42. B. Škrbić, J. Gyura, Iron, copper and zinc in white sugar from Serbian sugar beet refineries, Food

Control, 18, 135-139, 2007.

43. B. Škrbić, A. Onjia, Multivariate analyses of microelement contents in wheat cultivated in Serbia (2002), Food Control, 18, 338-345, 2007.

44. B. Škrbić, J. Cvejanov, N. Đurišić-Mladenović, Organochlorine residues in some the Serbian

agricultural products, Fresen. Environ. Bull., 16, 122-126, 2007.

45. B. Škrbić, J. Cvejanov, N. Đurišić-Mladenović, Organochlorine pesticides and polychlorinated biphenyls in surface soils of Novi Sad and bank sediment of the Danube River, J. Environ. Sci. Health, B42, 311-319, 2007.

46. B. Škrbić, B.Filipčev, Element intakes through the consumption of different types of bread by

Serbian population, Acta Alimentaria, 36, 217-229, 2007.

47. B. Škrbić, Organochlorine and organophosphate pesticide residues in wheat varieties from Serbia, Food Addit. Contam., 24, 695-703, 2007.

48. B. Škrbić, N. Đurišić-Mladenović, Distribution of chlorinated organic pollutants in a wide variety of

soils from Europe and Asia worldwide: A multivariate statistical approach, Arch. Environ. Contam. Toxicol., 52, 466-474, 2007.

49. B. Škrbić, N. Đurišić-Mladenović, Non-dioxin-like PCBs in crops and related products: levels and

intakes in Serbia, Food Addit. Contam., 24, 652-662, 2007.

50. B. Škrbić, N. Đurišić-Mladenović, Principal component analysis for soil contamination with organochlorine compounds, Chemosphere 68, 2144-2152, 2007.

51. B. Škrbić, Level of organochlorine pesticides and polychlorinated biphenyls in products of sugar

beet refineries in Serbia, Fresen. Environ. Bull., 16, 576-581, 2007.

48

52. B. Škrbić, A.Onjia, Prediction of programmed-temperature retention indices of polycyclic aromatic hydrocarbons in the Lee index scale by artificial neural network, MATCH Commun. Math. Comput. Chem., 55, 287-304, 2006.

53. B. Škrbić, J. Gyura, Survey on some contaminants in white sugar from Serbian sugar beet

refineries, Food Addit. Contam., 23, 31-35, 2006.

54. B. Škrbić, A.Onjia, Prediction of the Lee retention indices of polycyclic aromatic hydrocarbons by artificial neural network, J. Chromatogr. A, 1108, 279-284, 2006.

55. M.Šćiban, M.Klašnja, B.Škrbić, Modified hardwood sawdust as adsorbent of heavy metal ions

from water, Wood Sci. Technol., 40, 217-227, 2006.

56. M.Šćiban, M.Klašnja, B.Škrbić, Modified softwood sawdust as adsorbent of heavy metal ions from water, J. Hazard. Mater., B136, 266-271, 2006.

57. B. Škrbić, N. Đurišić-Mladenović, J. Cvejanov, Principal component analysis of trace elements in

Serbian wheat, J. Agric. Food Chem, 53, 2171-2175, 2005.

58. B. Škrbić, S. Čupić, Toxic and essential elements in soft wheat grain cultivated in Serbia, Eur. Food Res. Technol., 221, 361-366, 2005.

59. B. Škrbić, J. Cvejanov, N. Ðurišić-Mladenović, Polycyclic aromatic hydrocarbons in surface soils

of Novi Sad and bank sediment of the Danube River, J. Environ. Sci. Health A, 40, 29-43, 2005.

60. B. Škrbić, N. Ðurišić-Mladenović, Toxic and essential elements in Serbian sugar beet, molasses and white sugar, Zuckerindustrie, 130, 913-917, 2005.

61. E. Lončar, Lj.Kolarov, R. Malbaša, B. Škrbić, Qualitative TLC determination of some polycyclic

aromatic hydrocarbons in sugar-beet, J. Serb. Chem. Soc., 70, 1237-1242, 2005.

62. S. Sremac, B. Škrbić, A. Onjia, Artificial neural network prediction of quantitative structure – retention relationships of polycyclic aromatic hydrocarbons in gas chromatography, J. Serb. Chem. Soc., 70, 1291-1300, 2005.

63. B.Škrbić, A.Onjia, J.Cvejanov, N.Đurišić-Mladenović, S.Čupić, Relation between selected heavy

metal concentrations in wheat from growing regions of Serbia, J. Environ. Prot. Ecol., 6, 651-659, 2005.

64. B.Škrbić, S.Čupić, J.Cvejanov, A.Onjia, Determination and distribution of heavy metals in crops

harvested in 2001 from different parts of Serbia, J. Environ. Prot. Ecol., 5, 36-42, 2004.

65. D.Golobočanin, B.Škrbić, N.Miljević, Principal component analysis for soil contamination with PAHs, Chemom. Intell. Lab. Syst., 72, 219-223, 2004.

49

66. B. Škrbić, N. Đurišić- Mladenović, J. Cvejanov, Discrimination between linear and non-linear

models for retention indices of polycyclic aromatic hydrocarbons in the so-called Lees scale, Chemom. Intell. Lab. Syst., 72, 167-171, 2004.

67. B. Škrbić; S. Čupić, Trace metal distribution in surface soils of Novi Sad and bank sediment of

the Danube River, J. Environ. Sci. Health A, 39, 1547-1558, 2004.

68. L. Slavković, B.Škrbić, N.Miljević, A.Onjia, Principal component analysis of trace elements in industrial soils, Environ. Chem. Lett., 2, 105-108, 2004.

69. B.Škrbić, S.Čupić, J.Cvejanov, Determination of heavy metals in beet sugar samples from

Vojvodina Province, J. Environ. Prot. Ecology, 4, 657-661, 2003.

70. B. Škrbić, J.Novaković, N. Miljević, Mobility of heavy metals originating from bombing of industrial sites, J. Environ. Sci. Health, A 37, 7-16, 2002.

71. B. Škrbić, N. Miljević, An evaluation of residues at an oil refinery sites following fires,

J. Environ. Sci. Health, A 37, 1029-1039, 2002. 72. B. Škrbić, N. Miljević, Mobility of heavy metals originating from bombing of industrial sites in

Serbia, J. Environ. Prot. Ecology, 2, 874-881, 2001. 73. B.Škrbić, Comparison of methods for prediction of the retention data of aromatic hydrocarbons

on UCON LB 550X and on polydimethylsiloxane, Chromatographia, 47, 11/12, 721-723, 1998. 74. B.Škrbić, Unified retention concept - statistical treatment of Kovats retention index,

J.Chromatogr.A, 764, 257-264, 1997.

75. B.Škrbić, Lj.Pavić-Suzuki, Application of the unified retention data in environmental analysis, J. Serb. Chem. Soc., 62, 565-573, 1997.

76. B.Škrbić, Unified retention indices of hydrocarbons on dinonyl-phthalate phase, J. Serb. Chem.

Soc., 62, 575-579, 1997. 77. B.Škrbić, J.Cvejanov, Lj.Pavić-Suzuki, Contribution to the unified retention data of hydrocarbons

on squalane, Chromatographia, 42, 660-664, 1996. 78. B.Škrbić, Comparison of correlation for prediction of gas chromatographic separation of

alkylbenzenes, Chromatographia, 41, 183-186, 1995.

79. B.Škrbić, J.Cvejanov, Pressure drop determination in packed column for sulfur dioxide absorption, J.Serb.Chem.Soc., 60, 821-825, 1995.

80. B.Škrbić, J.Cvejanov, Liquid holdup determination in packed columns for sulfur dioxide

absorption, Sep. Purif.Tech., 8, 13-16, 1994.

50

81. B.Škrbić, Unified retention indices of some alkylbenzenes and bicyclic aromatic and related compounds on OV-101 and SE-30, J. Serb. Chem. Soc., 59, 153-155, 1994.

82. B.Škrbić, M.Vojinović-Miloradov, A contribution to the qualitative GC analysis of some non-

chlorinated xenobiotic chemicals in waste waters, Wat. Sci. Tech., 30, 91-93, 1994.

83. B.Škrbić, J.Cvejanov, Correlation of unified retention indices for OV-101 and squalane, Chromatographia, 35, 109-110, 1993.

84. B.Škrbić, J.Cvejanov, R.Paunović, Citrate process for SO2 recovery, Vapor-liquid data and

correlation, Sep. Purif.Tech., 7, 27-30, 1993.

85. B.Škrbić, J.Cvejanov, Unified retention indices of alkylbenzenes on OV-101 and SE-30, Chromatographia, 37, 215-217, 1993.

86. B.Škrbić, J.Cvejanov, Unified retention indices of hydrocarbons on BP-1 dimethylsiloxane

stationary phases, Chromatographia, 34, 83-84, 1992. 87. B.Škrbić, J.Cvejanov, Comparative analysis of methods for determination of calorific values of

natural gas mixture, Fuel Process Technol., 28, 307-314, 1991.

88. B.Škrbić, J.Cvejanov, R.Paunović, An extension of semiempirical gas-liquid equilibrium model for sulphur dioxide absorption in aqueous sodium citrate solution, Chem. Eng. Sci., 46, 3314-3317, 1991.

89. B.Škrbić, S.Joksimović-Tjapkin, The effect of solid particles on burning velocity of premixed flame:

critical review, Hung. J. Ind. Chem., 16, 393-405, 1988. 90. B.Škrbić, Note of inaccuracy of the particle track methods, J. Serb. Chem. Soc., 50, 419-425,

1985.

91. B.Škrbić, J.Cvejanov, M.Peruničić, Selection of mixing rule for the prediction of the laminar burning velocity for multicomponent mixture, Hung. J .Ind. Chem., 13, 199-208, 1985.

92. R.Paunović, M.Janković, B.Škrbić, An extended Ishii-Otto algorithm for multistage

multicomponent separation with stage efficiency included, Comput. Chem. Eng., 8, 249-252, 1984.

93. B.Škrbić, M.Zlatković, Simple method for the rapid analysis of natural gas by gas

chromatography, Chromatographia, 17, 44-46, 1983. Analytic Chemistry Application Review ‘85, 57 (5) 274 R (1985).

94. R.Paunović, M.Janković, B.Škrbić, A modified Ishii-Otto Method for multistage multicomponent

separation stage efficiencies included, Bull.Soc.Chim. Belgrade, 47, 67-74, 1982.

51

95. A.Tolić, M.Sovilj, Đ.Vataji, B.Škrbić, D.Petrović, Study of extraction characteristics of multicomponent system: selected oil distillate-furfurol, Bull.Soc.Chim. Belgrade, 47, 282-291, 1982.

96. R.Paunović, B.Škrbić, Prediction of the vapor pressure of pure substance by using an equation

of state, High Pressure-High Temperature, 11, 519-528, 1979.

97. R.Paunović, B.Škrbić, D.Miščević, A.Tolić, Selection of constants in BWR equation and its modification for the calculation of vapor-liquid equilibrium in mixtures of natural gas, Bull.Soc.Chim. Belgrade, 44, 291-296, 1979.

Invited lectures published in Proceedings

1. B. Škrbić, V.Marinković, V. Milanko, S. Spaić, A. Senderak, Benzene in combustion products and thermal decomposition products of poplar wood sawdust, Proceedings 4th International Conference Safety Engineering, Fire, Environment, Work Environment, Integrated Risk, 14 th International Conference Fire and Explosion Protection, p.411-419, Novi Sad, Serbia, 2-3 October 2014.

2. B. Škrbić, K. Héberger, Evaluation of models predicting retention indices for polycyclic aromatic

hydrocarbons by method based on sum of ranking differences, Proceeding of Joint Events - 2nd CEFSER (Center of Excellence for Food Safety and Emerging Risks) Workshop “Persistent organic pollutants in food and environment", 26th Symposium on Recent Developments in Dairy Technology, BIOXEN seminar “Novel approaches for environmental protection”, p.7-11, Novi Sad, Serbia, 8-10 September, 2011.

3. E. Sajben, L. Manczinger, J. Krisch, B. Škrbić, Cs. Vágvölgyi, Characterization of an

ethylenethiourea-degrading Pseudomonas strain, Proceeding of Joint Events - 2nd CEFSER (Center of Excellence for Food Safety and Emerging Risks) Workshop “Persistent organic pollutants in food and environment", 26th Symposium on Recent Developments in Dairy Technology, BIOXEN seminar “Novel approaches for environmental protection”, p.50-53, Novi Sad, Serbia, 8-10 September, 2011.

4. B. Škrbić, N.Đurišić-Mladenović, J. Cvejanov, Distribution of polycyclic aromatic hydrocarbons in

the environment, Proceedings of 9th International Conference on Fundamental and Applied Aspects of Physical Chemistry, p.613-620, Belgrade, Serbia, September 24-26, 2008.

5. B. Škrbić, J. Cvejanov,S.Čupić, Trace elements in soft winter wheat from Serbia,Proceeding of

6th Symposium of Romanian Academy-Branch Timisoara, Metal Elements in Environment, Medicine and Biology, Eds.: Z.Garban, P.Dragan et al., p.75-84, Timisoara, Romania, Novemeber 8-10, 2004.

6. B. Škrbić, N.Miljević, O.Nešković, M.Veljković, M.Pavlović, N. Đurišić, Distribution of metals in

soil at the oil refinery site, Proceeding of 4th International Symposium on Metal Elements in Environment, Medicine and Biology, Tome IV, 265-270, Timisoara, November 6-8, 2000.

53

Invited lectures published in Book of Abstracts

1. B. Škrbić, M. Petrović, D. Barcelo, Pharmaceutical environmental waters: occurrence and chemical analysis, Book of Abstracts of 17th International Symposium on Environmental Pollution and its Impact on Life in the Mediterranean Region, p.128, Istanbul, Turkey, September 28-October 1, 2013.

2. B. Škrbić, N. Đurišić-Mladenović, Z. Predojević, Liquid biofuels production and characterisation:

overview of the investigation, Book of Abstracts of 12th Eurasia Conference on Chemical Sciences, p.S4-OP4, Corfu, Greece, 16-21 April 2012.

3. B. Škrbić, N. Đurišić-Mladenović, M. Petrović, Activities in the laboratory regarding the safety

issues, International seminar of EU FP7 project FLAVOURE, New methods in assessment of food/feed quality and safety, Saku (Harjumaa), Estonia, 17-18.01.2012.

4. B. Škrbić, J. Hajšlová J. Živančev, S. Koprivica, Determination of mycotoxins in wheat samples

and related commodities, International seminar of EU FP7 project FLAVOURE, New methods in assessment of food/feed quality and safety, Saku (Harjumaa), Estonia, 17-18.01.2012.

5. B. Škrbić, Mycotoxins in cereal-derived products: occurrence and intakes in selected Serbian

commodities, Book of abstracts of 2nd FCUB ERA Workshop, Food Safety and Health Effects of Food, p.11, Belgrade, Serbia, 18-19 October, 2011.

6. B. Škrbić, K. Héberger, Evaluation of models predicting retention indices for polycyclic aromatic

hydrocarbons. Comparison of methods based on sum of ranking differences and pair-correlation, Book of Abstracts of Joint Events - 2nd CEFSER (Center of Excellence for Food Safety and Emerging Risks) Workshop “Persistent organic pollutants in food and environment", 26th Symposium on Recent Developments in Dairy Technology, BIOXEN seminar “Novel approaches for environmental protection”, p.6, Novi Sad, Serbia, 8-10 September, 2011.

7. Cs.Vagvolgyi, J. Krisch, B. Škrbić, L. Manczinger, Microbial degradation of pesticides in the

environment, Book of Abstracts of Joint Events - 2nd CEFSER (Center of Excellence for Food Safety and Emerging Risks) Workshop “Persistent organic pollutants in food and environment", 26th Symposium on Recent Developments in Dairy Technology, BIOXEN seminar “Novel approaches for environmental protection”, p.2, Novi Sad, Serbia, 8-10 September, 2011.

8. Cs. Vágvölgyi, B. Škrbić, J. Krisch, L. Manczinger, Remediation of pesticide-polluted soils by

microbial bioaugmentation, Book of abstracts of 12th Danube-Kris-Mures-Tisa (DKMT) Conference on Food, Environment and Health, p.31, Novi Sad, Serbia, September 14-15, 2010.

9. B. Škrbić, N. Đurišić-Mladenović, Formation of centre of excellence in food safety and emerging

risks, Workshop Specific methods for food safety and quality, Vinča-Belgrade, Serbia, September 21, 2010.

54

10. B. Škrbić, N. Đurišić-Mladenović, M. Zorić, J. Živančev, S. Milovac, Evaluation of heavy elements distribution in surface soils of Novi Sad, Book of abstracts of the Pre10 Conference, Protection and Restoration of the Environment X, p.144, Corfu, Greece, July 05-09, 2010.

11. B. Škrbić,Heavy elements in the environment and food matrices: empirical and chemometrical

approach – overview of research activities of the group from Faculty of Technology, Seminar Microanalytical techniques in applied Earth science, RESTCA, Belgrade, Serbia, February 18-26, 2010.

12. B. Škrbić,FP7 REGPOT projects as a way to bridge R&D transnational gaps: the CEFSER project,

Seminar Microanalytical techniques in applied Earth science, RESTCA, Belgrade, Serbia, February 18-26, 2010.

13. B.Škrbić, N.Đurišić-Mladenović, B.Filipčev, Estimation of population exposure to PCBs by crops

in Serbia, International Conference Food Convention, Novi Sad, Serbia, March 30-April 5, 2008.

14. B.Škrbić, J.Cvejanov, S. Čupić, Polycyclic aromatic hydrocarbons and trace element distribution in Novi Sad, Book of abstracts of the Sixth European Meeting on Environmental Chemistry, p.47, Belgrade, December 6-10, 2005.

15. B.Škrbić, S. Čupić,N.Đurišić-Mladenović, Intake of toxic and essential elements from wheat-

based products for the Serbian population, Short course on Medical Geology, Health and the Environment, Symposium of the Western University «Vasile Goldis», Arad, Romania, 5-8 May, 2005.

16. B.Škrbić, Unified Retention Concept - Statistical Interpretation of Kovats Retention Index,

Summary of Abstracts of 10th International Symposium Advances and Applications of Chromatography in Industry, p.74, Bratislava, June 30-July 4, 1996.

Webinar Library M. Godula, B. Škrbić, Rapid Analysis of Mycotoxins in Foods and Feed, Webinar Library, Food and BeverageSolutions, Thermo Scientific,http://info1.thermoscientific.com/content/CMD_KL_Webinars?kl=FT_&ce=E.13CMD.MS136.G038730.00&iq=IQLAAEGAAUFZZZMZZZ&gp=DME-Direct%20Mkt%20Email&&elq_mid=5176&elq_cid=1738085#&rid=7440

Projects

Project of Provincial Secretariat for Science and Technological Development: Influenceof anthropogenic activities on the urban contamination level: occurrence of xenobiotics in street dust and soil as an indicator of the air pollution, 2015.Coordinator: Prof. Dr. Biljana Škrbić

Joint cooperation with the University of Stuttgart: Influence of the wastewater discharge

on the recipient – Preliminary study on sustainable water management in the Danube Basin

(Serbia) – Determination of micropollutants, 2014. Coordinator: Prof. Dr. Biljana Škrbić

55

Project of Provincial Secretariat for Science and Technological Development:

Occurence of emerging pollutants in the environment and foodstuffs from the Serbian

market, 2014.Coordinator: Prof. Dr. Biljana Škrbić

Project ofSerbian Ministry of Education and Science: No. 172050, Development and

application of the advanced chromatographic and spectrometric methods in the analysis of

xenobiotics and their degradation pathways in biotic and abiotic matrices, 2011-2015.

Coordinator: Prof. Dr. Biljana Škrbić

Project of Provincial Secretariat for Science and Technological Development:

Estimation of chemical safety of market basket and population dietary exposure, 2011-2015.


Bilateral project within Programme of Serbian - Chinese Science and Technology

Cooperation, Human exposure assessment to heavy elements, phthalic acid esters and

persistent organic pollutants through air, water, dust and food, 2013 -2015. Coordinator of

the Serbian team: Prof. Dr. Biljana Škrbić

Project cofinanced by EU within Hungary-Serbia IPA Cross-border Co-operation

programme implemented within the 2007 – 2013 Europan Union financial framework under

the Instrument for Pre-accession Assistance (IPA), Development of an enzymological

(laccase-based) remediation product and technology (LACREMED ), HU - SRB

/1002/214/147, 2012 -2013. Coordinator of the Serbian team (Deputy Research Project

Manager): Prof. Dr. Biljana Škrbić

Bilateral project within Programme of scientific and technological cooperation between the

Republic of Serbia and the Kingdom of Spain, Advanced chromatographic and mass

spectrometric techniques in food chemical safety analysis, 2012-2013. Coordinator of the

Serbian team: Prof. Dr. Biljana Škrbić

COST Action TD 1203 – Food waste valorization for sustainable chemicals, materials and

fuels (EUBis), 2012-2016, participation

COST Action ES 1202 – Conceiving wastewater treatment in 2020 – Energetic,

environmental and economic challenges (Water_2020), 2012-2016, participation

COST Action ES 1403 - New and emerging challenges and opportunities in wastewater

reuse (NEREUS), 2014-2018, participation

FP7 project No. 229629, CEFSER - Reinforcing research potential in the Laboratory for

Chemical Contaminants at the Faculty of Technology towards the establishment of the

Center of Excellence in Food Safety and Emerging Risks, 2009-2012. Coordinator: Prof. Dr.

Biljana Škrbić

Bilateral project within Serbian-Portugal intergovermental S&T programme, Polycyclic

aromatic hydrocarbons and biogenic amines in smoked dry traditionally manufactured meat

products from Serbia and Portugal, 2011-2012. Coordinator of the Serbian team: Prof. Dr.

Biljana Škrbić

56

Bilateral project within Serbian–Croatian intergovermental S&T programme, Inorganic

and organic pollutants in urban areas, 2011-2012. Coordinator of the Serbian team: Prof. Dr.

Biljana Škrbić

Project cofinanced by EU within Hungary-Serbia IPA Cross-border Co-operation

programme implemented within the 2007 – 2013 Europan Union financial framework under

the Instrument for Pre-accession Assistance (IPA), Development of xenobiotic - degrading

bioaugmentation products (BIOXEN), HU - SRB /0901/214/150, 2010-2011. Coordinator of

the Serbian team (Deputy Project Manager): Prof. Dr. Biljana Škrbić

Bilateral project within Serbian– Hungarian intergovermental S&T programme,

Comparison of various analytical and chemometric methods, 2010-2011. Coordinator of the

Serbian team: Prof. Dr. Biljana Škrbić

Bilateral project within Serbian–Slovenian intergovermental S&T programme, Heavy

metals in the environment as a consequence of the anthropogenic activities, 2010-2011.

Coordinator of the Serbian team: Prof. Dr. Biljana Škrbić

Project ofSerbian Ministry for Science and Technological Development: No. 152001B,

Sources identification and correlations amongst the elements and organic compounds in

abiotic and biotic matrices: risk analysis and a contribution to the monitoring and

improvement of the environmental status, 2008-2010. Coordinator: Prof. Dr. Biljana Škrbić

Certificated reference materials – wheat flour and soil certificated contents of polycyclic

aromatic hydrocarbons, Carlsberg Srbija d.o.о., 2006./2007. Coordinator: Prof. Dr. Biljana

Škrbić

Project of Serbian Ministry for Science and Technological Development: No. BTN-

321004B, within National Biotechnology and Agriculture Program: Baked goods and flour

confectioneries with addition of industrial plant seed, 2006-2007.Coordinator: Prof. Dr.

Biljana Škrbić

Production of new certificated reference materials. Project awarded within competition of

The Best Tehnological Innovation, 2007. Coordinator: Prof. Dr. Biljana Škrbić

Project of Austrian Federal Ministry of Foreign Affairs within Course development plus

program implemented in Support to higher education in Serbia and Montenegro in

2005/2007 (2006). Coordinator: Prof. Dr. Biljana Škrbić

Project of Serbian Ministry for Science No. 1775, within Basic Research-Chemistry:

Development of methods for identification of chemical residues and contaminants in major

food crops, 2002-2005. Coordinator: Prof. Dr. Biljana Škrbić

Project ofSerbian Ministry for Science and Environment, No. 02E31: Development of

new technologies for wastegas and wastewater purification and methods for gas and water

quality control, 1996-2000. Coordinator: Prof. Dr. Biljana Škrbić

Project of Serbian Ministry for Science and Environment, No. 0935: Development of the

ecologically accepted processes and products in oil-petrochemical industry, 1991-1995.


57

President of the Scientific and Organizing Committee of:

Workshop on New emergency approach to the identification of targeted compounds in environmental issues, Novi Sad, Serbia, 8 June 2015.

Workshop on Valorisation of Vegetable Waste, COST Actions no. TD1203 EUBis, Novi Sad, Serbia, 6-7 August 2014.

Training Course onChemical Contaminants in the Environment, Novi Sad, Serbia, 21-23 July 2014.

LACREMED Training Course, Novi Sad, Serbia, 19-20 September, 2013.

LACREMED-Mid-term meeting, Novi Sad, 18. January 2013.

CEFSER Closing event and final training, Novi Sad, Serbia, 30 July 2012.

5th CEFSER Training Course - Analysis of chemical contaminants in food and the environment, Novi Sad, Serbia, May 7-11, 2012.

4th CEFSER Training Course - Persistent organic pollutants in food and environment: Risk assessment, Novi Sad, Serbia, November 14-15, 2011.

2nd CEFSER Workshop – Persistent organic pollutants in food and environment, Novi Sad, Serbia, September 8-10, 2011.

BIOXEN Seminar - Novel approaches for environmental protection, Novi Sad, Serbia, September 8-10,2011.

BIOXEN Training Course - High resolution mass spectrometry of xenobiotics, Novi Sad, Serbia, June 1-3, 2011.

The 3rdCEFSER Training Course – High resolution mass spectrometry in quantitative analysis and screening of organic contaminants in food and environment, Novi Sad, Serbia, September 16-17, 2010.

The 12th Danube-Kris-Mures-Tisa (DKMT) Euroregion Conference on Food, Environment and Health, Novi Sad, Serbia, September 14-15, 2010.

The 1st Center of Excellence for Food Safety and Emerging Risks Workshop, Regional perspective in food safety, Novi Sad, Serbia, September 14, 2010.

The 2ndCEFSER Training Course - Quality Assurance (QA) and Quality Control (QC) Procedures in Analysis of Contaminants and Pharmaceutical Compounds in Food and the Environment, Novi Sad, Serbia, April 9, 2010.

The 1st CEFSER Training Course – Capabilities of U-HPLC-MS/MS in Аnalysis of Contaminants and Pharmaceutical Compounds in Food and the Environment, Novi Sad, Serbia, April 6-8, 2010

FP7CEFSERСимпозијум „Значај преноса научних сазнања“, Технолошки факултет, Нови Сад, 30.11.2009.

The 5th Danube-Kris-Mures-Tisa (DKMT) Euroregion Conference on Environment and Food Quality, Novi Sad, Serbia and Montenegro, 4-5 September, 2003.

58

A U T H O R S’ I N D E X

A Antić, I. 18, 19, 20, 22, 24, 25,

32, 33, 34, 38, 41

B Bóka, B. 27 Buljovčić, M. 41

C Cvejanov, J. 21, 24, 35, 41

Č

Čelić, M. 33, 36

Đ Đurišić-Mladenović, N. 23, 32, 33, 34, 40

F Farre, M. 24, 31

Fraqueza, M. J. 23

H Hatvani, L. 28 Husemann, J. 38

J

Ji, Y. 25, 40, 41 Jovanović, G. 24 Jovanović, Lj. 27

K Kadokami, K. 33, 38 Kredics, L. 29 Kuch, B. 38

M Manczinger, L. 27, 28, 29 Marinković, V. 27, 28, 29, 34, 35, 41, 43

N Nikolić, N.. 33 Nita, L. 28, 29 P Palágyi, A. 28, 29 Panković, D. 27 Petanovska-Ilijevska, B. 17 Petrović, M. 31, 36 Petrović-Gegić, A. 34, 35, 43 Pucarević, M. 27

R Radulov, I. 28, 29

S

Schlichtig, B. 38

Š Škrbić, B. 15, 18, 19, 20, 21, 22 ,

23, 24, 25, 31, 32, 33, 34, 35, 36, 38, 40, 41

T Tadić, Đ. 32, 35, 38 Tóth, B. 29

V Vágvölgyi, Cs. 27, 28, 29 Varga, J. 29 Velkovska-Markovska, L. 17 Vidács, L. 28 Vörös, M. 27

Ž Živančev, J. 18, 19, 20, 21, 22, 24,

25, 32

CIP - Каталогизација у публикацији

Библиотека Матице српске, Нови Сад

502:614.8.027.1

WORKSHOP on approaches for risk assessment of emerging contaminants

with Round table "How to use EU fund" (2015 ; Novi Sad)

Book of abstracts [Elektronski izvor] / Workshop on approaches for risk

assessment of emerging contaminants with Round table "How to use EU fund",

16-17th November 2015, Novi Sad. - Novi Sad : Faculty of Technology, 2015. -

1 elektronski optički disk (CD-ROM) ; 12 cm

Registar.

ISBN 978-86-6253-050-9

a) Заштита животне средине - Загађења - Процена ризика - Зборници -

Апстракти

COBISS.SR-ID 300854279

http://www.vbs.rs/scripts/cobiss?command=DISPLAY&base=COBIB&RID=300854279#_blank

WORKSHOP ON APPROACHES FOR RISK ASSESSMENT OF … of Abstracts-Workshop on... · 2017-07-04 ·...

Documents

Transcript of WORKSHOP ON APPROACHES FOR RISK ASSESSMENT OF … of Abstracts-Workshop on... · 2017-07-04 ·...