Workshop jaca spain 2014_alfredo garcia fernandez

AdAptA project:

Population size and population origin for

marginal/peripheral populations

• Alfredo García Fernández

• Carlos Lara-Romero

• Javier Morente-López

• Mª Luisa Rubio Teso

• Jose Carmen Soto

Correa

• José M. Iriondo Alegría

• COST ACTION MaP JACA 2014.

Ideas & ObjetivesDesigns &

ExperimentsQueriesIntroduction

• High mountain ecosystems, highly endangered by global change.

• For many alpine species, Mediterranean mountains are southern limit of distribution

(glacial refugee).

• Upward/latitudinal migration is not an option.





(glacial refugee).

• Upward/latitudina migration is not an option.

• Adaptation as one of the main response against new environmental conditions.





(glacial refugee).

• Upward migration is not an option.

• Adaptation as one of the main response against new environmental conditions.

• Introduction of “altitude variable” in the consideration of Central - Periphery populations

e.g. One population in the southern distribution limit but at intermediate altitude could be

considered marginal population?

Local gradient VS Whole distribution.

Silene ciliata Pourret

• Perennial cushion plant.

• Mediterranean mountain plant

• Central System (Central Iberian Peninsula) southern

limit of species distribution.

• Small occupation area. Certain population isolation.

• Inhabit altitude range from 1900 to 2500 m.a.s.l.

o High phenotypic variability

o Population genetic

o Limited dispersal availability

o Demographic fitness



Not a forest species



Importance of gene flow and population size in evolutionary potential in marginal populations

FITNESSEvolutive potentialGenetic diversity

Marginal populations

Gene flow between populations of different size.BIG/SMALL

(Lemu & Fischer 2008)

Natural selection

Gene flow between populations CENTRER/PERIPHERY

(Sexton et. al. 2011)

?

?

OBJETIVES

Evaluate the effects of gene-flow between Center-Periphery VS Periphery-Periphery and

population size in the biological performance of individuals from marginal populations of S.

ciliata.

QUESTIONS:

(1) Can be improved the viability of marginal populations by gene flow with pollen from

populations that occur in the environmental optimum conditions of the species?

OR MAYBE

(2) Could be more effective the gene flow with pollen from other marginal populations that are

under similar selective pressures?

AND ALSO

(3) What is the influence of population size in the population viability?



Definition of centrer and periphery

A critical step for our approach is the definition of center and periphery populations



Ávila

SegoviaSalamanca

Madrid

• Environmental point of view

instead of geographic

• MAXENT approach with

environmental values that explain

the species distribution.

• Identification of center and

periphery populations

Definition of centrer and periphery

A critical step for our approach is the definition of center and periphery populations



GuadarramaGredosBéjar

• Central Big Population

• Peripheric Big Population

• Peripheric Small Population

EXPERIMENT 1:

1. In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-

populations).



Planteamiento Diseño PreguntasIntroducción

Periphery Big from other mountain range

Periphery Small A

Central Big A

Periphery Big A

• Each periphery population received pollen from itself, from the central of its same mountain range and from other peripheral of the same or bigger population size.

Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Periphery effects

• Plant cuttings from 20 mother plants in each population

Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations).

• Manual crossings.

• Seed sowing in situ in periphery populations:• 3 types of crossings.• Control of intrapopulation variability. • Block design to avoid environmental heterogeneity

• Production of clonal families from cuttings.



180 mother plants!

14400 cuttings!!!

18000 crosses!!!

Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations).



24000 SEEDS !!!!!!

Béjar

Guadarrama

Gredos

¿?

¿?

¿?

Experiment 2: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Full effects.



Periphery Small

Central Big

Periphery Big

Periphery Small

Central Big

Periphery Big

Periphery Small

Central Big

Periphery Big

Experiment 3: Transcriptome comparisons during the seedling stage



Periphery population

Central population

Seed collection Greenhouse sowing ARN extraction and sequencing

Plant ARN after

drought treatment

Plant ARN control

Some ideas…

• Experiment 1: Common garden approach, Periphery effects:

Sowing design.

• Experiment 2: Common garden approach, Full effects:

Following the same design as experiment 1, > 50,000 seeds.

• Experiment 3: Transcriptome approach:

Experience in NGS?

Drought treatment?

Pool individuals



Thanks for your attention

AdAptA project:

Population size and population origin for

marginal/peripheral populations

• Alfredo García Fernández

• Carlos Lara-Romero

• Javier Morente-López

• Mª Luisa Rubio Teso

• Jose Carmen Soto

Correa

• José M. Iriondo Alegría

• COST ACTION MaP JACA 2014.

Workshop jaca spain 2014_alfredo garcia fernandez

Science

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