Winnie Svendsen, DTU

www.nanotech.dtu.dk/nabis

Point-of-Care devices

Winnie E. Svendsen

DTU Nanotech


Point of Care - Health

http://www.continuaalliance.org/about-the-alliance


Point of Care – Health

http://health.rush.edu/HealthInformation/Care%20Guides/28/000278.aspx


Point of Care Technology

Ref: Center for Bio photonics Science and technology University of California


Point of Care – Enviroment

Vandforurening i Kalundborg Børnehave ramt af vandforurening på tredje uge Udgivet den 02.01.2013 21:18

Advarsel: Drik ikke vandet i KBH Fredag d. 19. aug. 2011 kl. 17:11

2013

2012

2011

Stadig forbud mod brug af vand i centrum af Kalundborg Udgivet den 20.12.2012 21:09 af TV2 ØST's Redaktion

http://www.tv2east.dk/artikler/boernehave-ramt-af-vandforurening-paa-tredje-uge

http://www.tv2east.dk/artikler/stadig-forbud-mod-brug-af-vand-i-centrum-af-kalundborg

http://www.tv2east.dk/user/12





Point of Care – Enviroment

The Law : Lab test • 30 test per year • 72 hours response time

http://www.bt.dk/sites/default/files-dk/node-images/451/4/4451884-vand.jpg


Nano Bio Integrated Systems (NaBIS )

2012

Micro and Nano Sensor Platforms

Microfluidic Sample handling and manipulation

Bio – Medical Applications

Our vision is to incorporate our research in point of care (POC) technology for diagnostic and monitoring. This is achieved by application-driven research, adapting and refining methodologies to micro- and nanotechnology within bio-medical fields


Nano Bio Integrated Systems (NaBIS )





Point of Care – Sensors





Nano Bio sensors - Health

Biomedical engineering for cancer

and brain disease diagnosis and therapy development

A screenning system for The plasmacytosis virus in Mink

http://www.engcabra.eu

http://www.engcabra.eu/


64 chips

4 SiNW on one chip

The Silicon Nano Wires Sensor

300 nm

SiNW h= 50 nm l = 200 µm w= 50 µm


SiNW h= 50 nm l = 200 µm w= 50 µm

Azeem Zulfigar


Semiconducting Nanowire Sensor

+ +

Acceptors Holes (mobile)

=> Get a chage in conductance (DG/G)

+ + + +

+

- - -

Receptors binding a target molecule (negative)

Know and can control charge carriers in the wire


Silicon Nanowire Biosensor

Nanowire

Add a functional layer

Multiple functional layers


Silicon Nanowire Biosensor


SiNW sensor for Aleutian Disease Virus (ADV)

Integration of Silicon Nanowires in Microfluidic Devices


SiNW sensor for Aleutian Disease Virus (ADV)

Aleutian Disease or Mink Plasmacytosis is a highly contagious parvovirus affecting the animals, causing spontaneous abortion and death in minks and ferrets. ADV smallest virus existing d~7 nm Task : Screen blood samples for antibodies


Immobilization of whole virus

Goal : Attach the dead virus on the surface

Detect antibodies in the animal sample

APTES


Test Functionalisation Scheme Immobilization results

12 hours without light

3 hours with P H3CBF4

Conclusion: 1.High selectivity 2.High Yield 3.The catalysts worked as planned

12 hours with light


Screening for ADV virus in Minks sample

Lars Andresen

KU Sund 2010


Michael Jørgensen Msc Martin H. Sørensen MSc

Integration of Silicon Nanowires in Microfluidic Devices

www.nanotech.dtu.dk/nabis www.nanotech.dtu.dk/nabis

Fully automated system


POCT Screening of Cancer

Biomedical engineering for cancer

and brain disease diagnosis and therapy development

CA9 NKG2D ligands


Functionalisation Scheme

APTES

Andrea DTU

Lars KU Sund


Mouse Antibody – Goat Antibody interaction


Sample Handling

Andrea Pfreundt DTU Nanotech


System Engineering/ Embedded Systems

Jan Madsen DTU Compute

Francois Patou DTU NTCH

www.nanotech.dtu.dk/nabis www.nanotech.dtu.dk/nabis

Acnowledgements People NaBIS

Funds

Collaborators

DTU Nanotecth Mads Brandbyge

Kristian Mølhave

KU-life Søren Schou

Lars Andresen

Franziska , Phd student

DTU Farma Åse Uttenthal

DPA Leif Bruun

Rebekka Dam-Tuxen, Phd Stud.

iSAS – Slovakia

Prof. Silvia Patrokova

DTU Compute Prof. Jan Madsen

http://en.fi.dk/

Winnie Svendsen, DTU

Technology

Transcript of Winnie Svendsen, DTU