Wild Type Drosophila
description
Transcript of Wild Type Drosophila
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Wild Type Drosophila
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Ommatidium
8 Photoreceptor cells (R1-R8)4 Cone cells (secrete lens)8 Pigment cells
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Wild Type Eye Phenotype
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Eyeless Mutant Phenotype (hypomorphic allele)
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DNA
Paired Domain Protein
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Figure 1A
LacZ
No Transcription of LacZ
Gal4 Enhancer-Trap Method
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Figure 1A
LacZ
Enhancer that controls expression in specific tissues
Gal4 Enhancer-Trap Method
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Figure 1A
LacZ
Tissue-specific Expressionof Gal4
Gal4 Enhancer-Trap Method
Tissue-specific Expressionof LacZ
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Figure 1
Eye-Antennal disc wing disc leg disc
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Figure 1A
LacZ
Gal4 Enhancer-Trap Method
Enhancer that controls expression in imaginaldisc cells
Transcription of LacZ in imginal disc cells
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Figure 1A
Enhancer that controls expression in imaginaldisc cells
Transcription of eyeless in imginal disc cells
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Figure 2
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Figure 3
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DNA
Paired Domain Protein
Drosophila ey, mouse Pax-6 (sey) and Human Pax-6 (Aniridia) genesare ~ 94% similar in Paired domain
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Enhancer that controls expression in imaginaldisc cells
Transcription of Pax-6 in imginal disc cells
Pax-6
Ectopic Expression of Mouse Pax-6 Gene
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Figure 5
Ectopic Expression of Mouse Pax-6 Gene
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Aniridia -- Human Pax-6 Mutation
Absence of the iris
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Xenopus Oocyte
Animal Pole
Vegetal Pole
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Xwnt-11 mRNA Localization
Xwnt-11 is Drosophila wingless homogue
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Normal
Ventralized
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Embryo Grafting Experiment
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Goosecoid ProteinExpression
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Embryo Grafting Experiment
InjectmRNAs
ForChordin etc.
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Formation of ventral structures
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Normal
Dorsalized
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