WHAT’S NEW! Cell Lysis Reagents
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We Are Here For You! PURIFY CLONE REPEAT ANALYZE Clone. Purify. Analyze. Save 30% when you purchase any 3 featured solutions Enter promo code RCLO12 From now till 31 August 2020 Reverse Transcription Product Name Size Cat. No. SuperScript ™ IV CellsDirect ™ cDNA Synthesis Kit 50 reactions 11750150 500 reactions 11750350 SuperScript ™ IV First-Strand Synthesis System 50 reactions 18091050 200 reactions 18091200 SuperScript ™ IV One-Step RT-PCR System 25 reactions 12594025 100 reactions 12594100 SuperScript ™ IV One-Step RT-PCR System with ezDNase ™ 25 reactions 12595025 100 reactions 12595100 SuperScript ™ IV Reverse Transcriptase 2,000 units 18090010 SuperScript ™ IV VILO ™ Master Mix 50 reactions 11756050 500 reactions 11756500 Hi-Fidelity DNA Polymerase Product Name Size Cat. No. Platinum ™ SuperFi II DNA Polymerase 100 Reactions 12361010 500 Reactions 12361050 Platinum ™ SuperFi II Master Mix 100 Reactions 12368010 500 Reactions 12368050 TOPO Cloning Kit/ System Product Name Size Cat. No. Bac-to-Bac ™ C-His TOPO ™ Expression System 20 reactions A11100 Bac-to-Bac ™ HBM TOPO ™ Secreted Expression System 20 reactions A11339 Bac-to-Bac ™ N-His TOPO ™ Expression System 20 reactions A11101 Champion ™ pET100 Directional TOPO ™ Expression Kit with BL21 Star ™ (DE3) One Shot ™ Chemically Competent E. coli 20 reactions K10001 pBlue TOPO ™ TA Expression Kit 20 reactions K483101 pcDNA ™ 3.1/V5-His TOPO ™ TA Expression Kit 40 reactions K480040 *More TOPO cloning kits available on thermofisher.com/save30 Competent Cells - Cloning / Expression Product Name Size Cat. No. MAX Effciency ™ Stbl2 ™ Competent Cells 5 x 200 µL 10268019 MAX Effciency ™ DH5α ™ Competent Cells 10 reactions 18258012 One Shot ™ BL21(DE3) Chemically Competent E. coli 20 x 50 µL C600003 *More cells types & formats available on thermofisher.com/save30 DNA⁄RNA Purification Product Name Size Cat. No. iPrep ™ PureLink ™ Total RNA Kit 1 kit IS10006 PureLink ™ 96 HQ Plasmid DNA Purification Kit 4 x 96 preps K210096 PureLink ™ Expi Endotoxin-Free Maxi Plasmid Purification Kit 10 preps A31217 PureLink ™ Genomic DNA Mini Kit 50 preps K182001 PureLink ™ HiPure Plasmid Miniprep Kit 25 preps K210002 PureLink ™ HQ Mini Plasmid DNA Purification Kit 100 preps K210001 PureLink ™ miRNA Isolation Kit 25 preps K157001 PureLink ™ PCR Micro Kit 50 preps K310050 PureLink ™ PCR Purification Kit 50 preps K310001 PureLink ™ Quick Gel Extraction Kit 50 preps K210012 PureLink ™ Quick Plasmid Miniprep Kit 50 preps K210010 PureLink ™ RNA Mini Kit 50 preps 12183018A Cell Lysis Reagents Product Name Size Cat. No. B-PER ™ Complete Bacterial Protein Extraction Reagent 500 mL 89822 Pierce ™ IP Lysis Buffer 250 mL 87788 RIPA Lysis and Extraction Buffer 250 mL 89901 Subcellular Protein Fractionation Kit for Cultured Cells 35 mL 78840 Pro-Detect Rapid Assay Kit Product Name Size Cat. No. Pro-Detect ™ Rapid GST Assay Kit 10 assays A38502 Pro-Detect ™ Rapid MBP Assay Kit 10 assays A38503 Pro-Detect ™ Rapid SUMO Assay Kit 10 assays A38501 Pro-Detect ™ Rapid AVI Competitive Assay Kit 10 assays A38511 Pro-Detect ™ Rapid HA Competitive Assay Kit 10 assays A38509 Pro-Detect ™ Rapid Human Fc Assay Kit 10 assays A38504 Pro-Detect ™ Rapid Mouse Fc Assay Kit 10 assays A38506 Pro-Detect ™ Rapid Myc Competitive Assay Kit 10 assays A38512 Pro-Detect ™ Rapid Rabbit Fc Assay Kit 10 assays A38505 Pro-Detect ™ Rapid Streptag II Competitive Assay Kit 10 assays A38510 Pro-Detect ™ Rapid V5 Competitive Assay Kit 10 assays A38513 Protein Ladders Product Name Size Cat. No. PageRuler ™ Prestained Protein Ladder, 10 to 180 kDa 2 x 250 µL 26616 PageRuler ™ Plus Prestained Protein Ladder, 10 to 250 kDa 2 x 250 µL 26619 PageRuler ™ Prestained NIR Protein Ladder 2 x 250 µL 26635 Pre-Cast Protein Gels Product Name Bolt ™ Bis-Tris Plus Gels Novex ™ Tricine Protein Gels Novex ™ Tris-Glycine Mini Gels, WedgeWell ™ format Novex ™ Tris-Glycine Plus Midi Gels, 12+2-well Novex ™ Zymogram Plus (Gelatin) Protein Gels NuPAGE ™ Bis-Tris Midi Protein Gels, 12+2-well NuPAGE ™ Bis-Tris Protein Gels NuPAGE ™ Tris-Acetate Midi Protein Gels, 12+2-well NuPAGE ™ Tris-Acetate Protein Gels *Full list of gel types & well formats on thermofisher.com/save30 Product Name Size Cat. No. No-Stain ™ Protein Labeling Reagent 40 reactions A44449 NuPAGE ™ Large Protein Staining Kit 1 kit LP0002 Chemiluminescent Substrates Product Name Size Cat. No. SuperSignal ™ West Atto Ultimate Sensitivity Substrate 20 mL A38554 100 mL A38555 Endotoxin Detection & Removal Product Name Size Cat. No. Pierce ™ Chromogenic Endotoxin Quant Kit 30 reactions A39552S Pierce ™ High Capacity Endotoxin Removal Resin 100 mL 88271 Pierce ™ High Capacity Endotoxin Removal Spin Columns, 1 mL 5 columns 88276 NEW NEW NEW NEW NEW NEW NEW NEW Need help with your Western Blots? Optimize your protocol with our BlotBuilder tool now! Which polymerase fits you best? Find out using our Polymerase Selection Guide! For Research Use Only. Not for use in diagnostic procedures. © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. This promotion is open only to customers in Singapore. In order to obtain 30% discount, customers have to purchase 3 of any of our featured products on thermofisher.com/save30. In order to redeem the discount, the promotion code RCLO12 must be presented at time of order no later than 31 August, 2020, or before promotional supplies are depleted, whichever comes first. Health care professionals may not participate in this promotion. The promotion cannot be combined with products that are already in the pricing agreement and other promotions. The Company reserves the right to change these terms and conditions at any time without prior notice. For more information on this promotion, log on to thermofisher.com/save30 Exceptional Fidelity Combined with a Universal Annealing Temperature Find out more at thermofisher.com/platinumsuperfi Platinum ™ SuperFi II DNA Polymerase • Exceptional >300X Taq fidelity • Universal primer annealing at 60°C • Robust amplification of difficult-to-amplify targets PCR Visualization of Proteins in Gels within 10 Minutes Find out more at thermofisher.com/no-stain No-Stain ™ Protein Labeling Reagent • Lower limit of detection of 20 ng per band • Compatible with any gel type (precast or pour-your-own gel) using any commonly available imagers • Accurate normalization over a wide range of protein lysate concentrations (1–80 µg) SDS-PAGE Ultra-Sensitive Protein Detection at High-Attogram Level Find out more at thermofisher.com/supersignal SuperSignal ™ West Atto Ultimate Sensitivity Chemiluminescent Substrate • High sensitivity detection of proteins from low-femtogram to high-attogram • High stability - 48 hours for working solution; one year for kit at 4°C • Signal duration: up to six hours Western Blot WHAT’S NEW! Protein Visualisation
Transcript of WHAT’S NEW! Cell Lysis Reagents
We Are Here For You!
PURIFYCLONE
REPEATANALYZE
Clone. Purify. Analyze. Save 30% when you purchase any 3 featured solutions
Enter promo code RCLO12From now till 31 August 2020
Reverse TranscriptionProduct Name Size Cat. No.
SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
50 reactions 11750150
500 reactions 11750350
SuperScript™ IV First-Strand Synthesis System
50 reactions 18091050
200 reactions 18091200
SuperScript™ IV One-Step RT-PCR System
25 reactions 12594025
100 reactions 12594100
SuperScript™ IV One-Step RT-PCR System with ezDNase™
25 reactions 12595025
100 reactions 12595100
SuperScript™ IV Reverse Transcriptase 2,000 units 18090010
SuperScript™ IV VILO™ Master Mix50 reactions 11756050
500 reactions 11756500
Hi-Fidelity DNA Polymerase Product Name Size Cat. No.
Platinum™ SuperFi II DNA Polymerase100 Reactions 12361010
500 Reactions 12361050
Platinum™ SuperFi II Master Mix 100 Reactions 12368010
500 Reactions 12368050
TOPO Cloning Kit/ SystemProduct Name Size Cat. No.
Bac-to-Bac™ C-His TOPO™ Expression System
20 reactions A11100
Bac-to-Bac™ HBM TOPO™ Secreted Expression System
20 reactions A11339
Bac-to-Bac™ N-His TOPO™ Expression System
20 reactions A11101
Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
20 reactions K10001
pBlue TOPO™ TA Expression Kit 20 reactions K483101
pcDNA™3.1/V5-His TOPO™ TA Expression Kit
40 reactions K480040
*More TOPO cloning kits available on thermofisher.com/save30
Competent Cells - Cloning / ExpressionProduct Name Size Cat. No.
MAX Effciency™ Stbl2™ Competent Cells 5 x 200 µL 10268019
MAX Effciency™ DH5α™ Competent Cells 10 reactions 18258012
One Shot™ BL21(DE3) Chemically Competent E. coli
20 x 50 µL C600003
*More cells types & formats available on thermofisher.com/save30
DNA ⁄RNA Purification Product Name Size Cat. No.
iPrep™ PureLink™ Total RNA Kit 1 kit IS10006
PureLink™ 96 HQ Plasmid DNA Purification Kit
4 x 96 preps K210096
PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit
10 preps A31217
PureLink™ Genomic DNA Mini Kit 50 preps K182001
PureLink™ HiPure Plasmid Miniprep Kit 25 preps K210002
PureLink™ HQ Mini Plasmid DNA Purification Kit
100 preps K210001
PureLink™ miRNA Isolation Kit 25 preps K157001
PureLink™ PCR Micro Kit 50 preps K310050
PureLink™ PCR Purification Kit 50 preps K310001
PureLink™ Quick Gel Extraction Kit 50 preps K210012
PureLink™ Quick Plasmid Miniprep Kit 50 preps K210010
PureLink™ RNA Mini Kit 50 preps 12183018A
Cell Lysis ReagentsProduct Name Size Cat. No.
B-PER™ Complete Bacterial Protein Extraction Reagent
500 mL 89822
Pierce™ IP Lysis Buffer 250 mL 87788
RIPA Lysis and Extraction Buffer 250 mL 89901
Subcellular Protein Fractionation Kit for Cultured Cells
35 mL 78840
Pro-Detect Rapid Assay Kit Product Name Size Cat. No.
Pro-Detect™ Rapid GST Assay Kit 10 assays A38502
Pro-Detect™ Rapid MBP Assay Kit 10 assays A38503
Pro-Detect™ Rapid SUMO Assay Kit 10 assays A38501
Pro-Detect™ Rapid AVI Competitive Assay Kit
10 assays A38511
Pro-Detect™ Rapid HA Competitive Assay Kit
10 assays A38509
Pro-Detect™ Rapid Human Fc Assay Kit 10 assays A38504
Pro-Detect™ Rapid Mouse Fc Assay Kit 10 assays A38506
Pro-Detect™ Rapid Myc Competitive Assay Kit
10 assays A38512
Pro-Detect™ Rapid Rabbit Fc Assay Kit 10 assays A38505
Pro-Detect™ Rapid Streptag II Competitive Assay Kit
10 assays A38510
Pro-Detect™ Rapid V5 Competitive Assay Kit 10 assays A38513
Protein Ladders Product Name Size Cat. No.
PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
2 x 250 µL 26616
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
2 x 250 µL 26619
PageRuler™ Prestained NIR Protein Ladder
2 x 250 µL 26635
Pre-Cast Protein GelsProduct Name
Bolt™ Bis-Tris Plus Gels
Novex™ Tricine Protein Gels
Novex™ Tris-Glycine Mini Gels, WedgeWell™ format
Novex™ Tris-Glycine Plus Midi Gels, 12+2-well
Novex™ Zymogram Plus (Gelatin) Protein Gels
NuPAGE™ Bis-Tris Midi Protein Gels, 12+2-well
NuPAGE™ Bis-Tris Protein Gels
NuPAGE™ Tris-Acetate Midi Protein Gels, 12+2-well
NuPAGE™ Tris-Acetate Protein Gels
*Full list of gel types & well formats on thermofisher.com/save30
Product Name Size Cat. No.
No-Stain™ Protein Labeling Reagent 40 reactions A44449
NuPAGE™ Large Protein Staining Kit 1 kit LP0002
Chemiluminescent SubstratesProduct Name Size Cat. No.
SuperSignal™ West Atto Ultimate Sensitivity Substrate
20 mL A38554
100 mL A38555
Endotoxin Detection & Removal Product Name Size Cat. No.
Pierce™ Chromogenic Endotoxin Quant Kit 30 reactions A39552S
Pierce™ High Capacity Endotoxin Removal Resin
100 mL 88271
Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL
5 columns 88276
NEW
NEW
NEW
NEW
NEW
NEW
NEW
NEW
Need help with your Western Blots?
Optimize your protocol with our
BlotBuilder tool now!
Which polymerase fits you best?
Find out using our
Polymerase Selection Guide!
For Research Use Only. Not for use in diagnostic procedures. © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
This promotion is open only to customers in Singapore. In order to obtain 30% discount, customers have to purchase 3 of any of our featured products on thermofisher.com/save30. In order to redeem the discount, the promotion code RCLO12 must be presented at time of order no later than 31 August, 2020, or before promotional supplies are depleted, whichever comes first. Health care professionals may not participate in this promotion. The promotion cannot be combined with products that are already in the pricing agreement and other promotions. The Company reserves the right to change these terms and conditions at any time without prior notice.
For more information on this promotion, log on to thermofisher.com/save30
Exceptional Fidelity Combined with a Universal Annealing Temperature
Find out more at thermofisher.com/platinumsuperfi
Platinum™ SuperFi II DNA Polymerase
• Exceptional >300X Taq fidelity• Universal primer annealing at 60°C• Robust amplification of difficult-to-amplify targets
PC
R
Visualization of Proteins in Gels within 10 Minutes
Find out more at thermofisher.com/no-stain
No-Stain™ Protein Labeling Reagent
• Lower limit of detection of 20 ng per band• Compatible with any gel type (precast or pour-your-own gel) using any commonly available imagers• Accurate normalization over a wide range of protein lysate concentrations (1–80 µg)S
DS
-PA
GE
Ultra-Sensitive Protein Detection at High-Attogram Level
Find out more at thermofisher.com/supersignal
SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate
• High sensitivity detection of proteins from low-femtogram to high-attogram• High stability - 48 hours for working solution; one year for kit at 4°C• Signal duration: up to six hours
West
ern
Blo
tWHAT’S NEW!
Protein Visualisation
Buy 3 for 30% OFF Visit thermofisher.com/save30 for all participating products
PositiveC C
T
NegativeT
Detect
Discover new
Thermo Scientifi c™ Pro-Detect™
rapid assay kits and rapid
competitive assay kits
• Enables accurate, qualitative confi rmation of expressed tagged-proteins within 10–15 minutes
• Detect directly from cell culture media, lysate, or purifi ed protein
• No special equipment needed
• Ideal for monitoring at all phases of protein expression in real time
SuperScript IV Reverse
Transcriptase
Platinum SuperFi II DNA Polymerase
TOPO PCR Cloning
MAX Efficiency™ DH5α Competent
Cells PureLink™ HiPure Plasmid
Miniprep Kit
B-PER™ with Enzymes Bacterial
Protein Extraction Kit
Pro-Detect Rapid Assay Kit
PageRuler™ Plus Prestained Protein
Ladder, 10 to 250 kDa
NuPAGE Bis-Tris Gels
No-Stain™ Protein Labeling Reagent SuperSignal West
Atto Ultimate Sensitivity
Chemiluminescent Substrate
Pierce Chromogenic Endotoxin Quant Kit
Pierce High Capacity Endotoxin Removal
Reisin
NEW
NEW
NEW
• Fast: Reduction of incubation time from >50 minutes to 10 minutes
• Superior: Significantly improved inhibitor resistance and better processivity
• Exceptional fidelity: >300x Taq
• Universal primer annealing at 60°C
• Fast: 5-min reaction at RT
• High cloning efficiency:
Up to 95%
• High transformation efficiency: Up to 1 × 109 transformants/µg plasmid DNA
• High: Purity plasmid DNA in
<2 hrs
• Efficient: Increased yields (up to 30μg), with low endotoxin levels (0.1-1 EU/ μg)
• Ready-to-use :Just add and incubate to recover soluble proteins in <15 mi
• Convenient: Ready-to-use format for direct gel loading
• Fast: Visualize and normalize proteins (down to 20ng per band) in 10 min
• Convenient: Compatible with all gel types and common imagers in the lab
• Fast: Confirm expression of your tagged protein within 5-10 min
• Sensitive: Down to low µg/mL concentrations
• Better protein integrity: neutral pH minimizes protein modifications
• Optimal protein separation: wide range of molecular weight separation
• Highly sensitive: Detect low-
abundance proteins from low-femtogram to high-attogram levels
• Detects 0.01 EU/ mL to 1 EU/mL in as little as 20min
• Eliminate >99% endotoxins with ≥85% protein recovery in 1hr
Reverse Transcription
Polymerase Chain Reaction
Vector Construction
TransformationPlasmid
ExtractionProtein
ExtractionConfirmation
Endotoxin Test & Removal
Downstream Applications
Western Blotting
Protein Gel Electrophoresis
