What are the three steps in PCR?

What are the three steps in PCR?

DenaturationHybridization of Primer

DNA replication

What is the enzyme used for PCR?

What is the enzyme used for PCR?

DNA Polymerase from a hyperthermophile

This is why the enzyme is not destroyed at 94 degrees Celsius

What are introns?

What are introns?

Introns are non-coding sequences found in structural genes in eukaryotes

They are removed during splicing

What is reverse transcriptase?

What is reverse transcriptase?

It is an enzyme that copies RNA into DNA

It is produced by certain viruses

What are restriction enzymes?

What are restriction enzymes?

They are enzymes that cut DNA at specific base sequences

They are produced by bacteria Many of them cut the DNA so that

they produce “sticky ends” or single stranded ends

What are vectors used in making recombinant DNA?

What are vectors used in making recombinant DNA?

Vectors are self replicating pieces of DNA

They are used to get DNA into cells Two commonly used vectors are

plasmids and bacteriophage

What are three techniques used to get recombinant DNA into cells?

What are three techniques used to get recombinant DNA into cells?

Transformation Electroporation Microinjection (for eukaryotic cells)

What does RFLP stand for?

What does RFLP stand for?

Restriction fragment length polymorphism

It is often used in DNA fingerprinting

It requires gel electrophoresis which separates DNA by size