Welcome to the Jungle - Targeted Therapies, Immunomodulatory Approaches, and the New Drugs in HNSCC...
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Transcript of Welcome to the Jungle - Targeted Therapies, Immunomodulatory Approaches, and the New Drugs in HNSCC...
Welcome to the Jungle - Targeted Therapies, Immunomodulatory Approaches, and the New
Drugs in HNSCC
Ezra E. W. CohenMoores Cancer Center
University of California San Diego
“Welcome to the jungle
We've got fun 'n' games
We got everything you want...”
GNR
Incidence
• 2014, US:–~50000 new cases –~8000 deaths
• Worldwide:–500000 new cases–6th leading cause of death
• M:F = 3:1
Ten Leading Cancer Types, United States, 2012
CA Cancer J Clin 2012 Jan-Feb;62(1):10-29.
Risk Factors
• Age (majority of patients >50 years old)• Tobacco • Alcohol• Viral
– Epstein-Barr virus (NPC)– HPV (oropharynx)
• Hereditary (rare):– Family history increases risk (3.5X)– Rare genetic syndromes, e.g. Fanconi’s Anemia
HPV
Cancers with Increasing Incidence Trends in the US
• CA Cancer J Clin. 2012 Jan 4.
Three-dose HPV vaccination coverage among girls (13 to 17 years), by state, 2010.
New (HPV)–associated cancers overall, and by sex, in the United States, 2009
• JNCI 2013 Feb 6;105(3):175-201
BehaviorDiagnosis of HPV-SCCHN(case-case)
Risk of HPV-SCCH
(case-control)
Number of sexual partners + +
History of oral-genital sex + +
History of anal cancer + +
Spouse with tonsil cancer + +
Cervical CIS or cancer Assoc Assoc
Sexual Behaviors and HNC
HNC HPV
• Strong association with oropharynx, especially tonsil, cancers
• Non-smokers, non-drinkers• Younger age• M:F = 3:1• 90% of HNC cases with HPV are sub-type
16
Prognostic effect of HPV
• Langer CJ. Exploring biomarkers in head and neck cancer. Cancer. 2012 Aug 15;118(16):3882-92.13
Human Papillomavirus and Overall Survival After
Progression of Oropharyngeal Squamous Cell Carcinoma
C Fakhry et al
15
Overall survival after disease progression
Sur
viva
l (%
)
0
25
50
75
100
Years after Progression0 1 2
No. at Riskp16-positivep16-negative
10576
6127
5119
p16-positive54.6%
p16-negative 27.6%
p<0.001
DE-INTENSIFICATION…as an approach to address
HPV related disease and good prognosis
E1308 Induction followed by IMRT/Cetuximab
INDUCTION
(3 cycles)
Paclitaxel 90 mg/m2 q 7d
CDDP 75mg/m2 q21d
Cetuximab 250mg/m2
qwk
ELIGIBILITY
Stage III/IVa,b
Resectable
HPV+ Oropharynx
CONCURRENT
IMRT 69.3Gy/33fxs
Cetuximab 250mg/m2 qwk
CR*
<CR
CONCURRENT
IMRT 54Gy/30 fxs
Cetuximab 250mg/m2
qwk
Cetuximab loading dose = 400mg/m2 on Day1 of Cycle1 with Induction
* CR indicates clinical CR and in patients with near CR will undergo biopsies of primary site to confirm pathological CR
SI
MULATION
Phase II Randomized Trial of Transoral Surgical Resection followed by Low-dose or Standard-dose IMRT in Resectable p16+ Locally Advanced Oropharynx Cancer (E3311)
p16+, Stage III/IV (cT1-2N1-N2b) OPSCC Credentialing of surgeon required as part of site
participation in the trial Stratify by stage and smoking status
T1-4a, N1-3HPV+ OPSCC
Amenable to TORS
BIOPSY
Nmax = 54
Cisplatin 75 mg/m2/q3wkPaclitaxel 90 mg/m2/week
BYL719 daily
TORS, SLND
Induction Chemotherapy3 cycles
9 weeks
pCR or pT1-2 N0-1
(-) margin, no ECE
Close margin, ≥pN2, or PNI/LVI
(+) margin, ECE
Observe
60 Gy IMRT
66 Gy IMRT
+ Weekly
Cisplatin
Risk-Stratified IMRT
TORS
* *
*FDG/PET-CT scan
Arm 1
Arm 2
Arm 3
Trial Schema
Treatment Overview
• Early stage (I, II): single modality – RT or surgery– 80-90% long-term survival
• Advanced stage (III, IVA, IVB): multi-modality therapy – surgery/RT/chemotherapy– 50% long term survival
• Recurrent/Metastasis– 15% can be salvaged (surgery, re-RT)– Palliative systemic therapy
EGFR Directed Therapy
Stratify by Karnofsky score:
90-100 vs. 60-80 Regional Nodes:
Negative vs. Positive Tumor stage:
AJCC T1-3 vs. T4 RT fractionation:
Concomitant boostvs. Once dailyvs. Twice daily
Arm 2
Radiation therapy
Cetuximab, weekly
Cetuximab Phase III Study
RANDOI
MIZE
Arm 1
Radiation therapy
Bonner. N Engl J Med. 2006;354:567. Copyright © [2006] Massachusetts Medical Society. All rights reserved.; Posner. N Engl J Med. 2006;354:634.
Phase III: Cetuximab Plus RT for SCCHN: Results
47% vs 34% at 3 yearsP<0.01 at 3 years
55% vs 45% at 3 yearsP=0.05 at 3 years
Locoregional Control OS
EXTREME - Study design
Group ACetuximab 400 mg/m2 initial dose
then 250 mg/m2 weekly + EITHER carboplatin (AUC 5, d1) OR cisplatin (100 mg/m2 IV, d1)+ 5-FU (1000 mg/m2 IV, d1-4):
3-week cycles
Group B
EITHER carboplatin (AUC 5, d1) OR cisplatin (100 mg/m2 IV, d1)
+ 5-FU (1000 mg/m2 IV, d1-4):3-week cycles
No treatmentCetuximab
Randomized
Progressive disease or unacceptable toxicity
6 chemotherapy cycles maximum
Patients at Risk Survival Time [Months]CTX onlyCET + CTX
220 173 127 83 65 47 19 8 1222 184 153 118 82 57 30 15 3
HR (95%CI): 0.797 (0.644, 0.986)Strat. log-rank test: 0.0362
Overall Survival
CTX onlyCET + CTX
Su
rviv
al P
rob
ab
ility
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
0 3 6 9 12 15 18 21 24
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10.1 mo7.4 mo
Afatinib versus methotrexate as second-line treatment for patients with R/M HNSCC who progressed after platinum-based therapy:
primary efficacy results of LUX-Head & Neck 1, a Phase III trial
J-P. H. Machiels, R. I. Haddad, J. Fayette, L. F. Licitra, M. Tahara, J. B. Vermorken, P. M. Clement, T. Gauler, D. Cupissol, J. J. Grau,
J. Guigay, F. Caponigro, G. de Castro Jr, L. de Souza Viana, U. Keilholz, J. M. del Campo, X. Cong, L. Svensson, E. Ehrnrooth,
and E. E. W. Cohen on behalf of the LUX-H&N 1 investigators
LUX-Head & Neck 1: study design
Primary endpoint: PFS
Key secondary endpoint: OSSecondary endpoints: ORR, patient-reported outcomes, safety
Randomisation (2:1)Stratified by: ECOG PS (0 vs 1) and
prior use of EGFR mAb therapy (Yes/No)
Afatinib 40 mg orally once daily
(n=316)
Methotrexate40 mg/m2 IV weekly
(n=158)
Patients with incurable R/M HNSCC progressing on/after first-line
platinum-based therapy (N=474)
Primary endpoint: PFS independent review
CI, confidence interval; MTX, methotrexate
Time (months)
0
0.2
0.4
0.6
0.8
1.0
0 3 6 9 12
Est
ima
ted
PF
S p
rob
abil
ity
15 18
No. of patientsAfatinib 322 93 26 9 3 1 0MTX 161 28 6 2 0 0 0
Afatinib(n=322)
MTX(n=161)
PFS event, n (%) 275 (85.4) 135 (83.9)Median PFS (months) 2.6 1.7HR (95% CI) 0.80 (0.65–0.98)Log-rank test p-value 0.030
42.8%
30.5%
afatinib mtx0
10
20
30
40
50
60
10.2
49.1
5.6
38.5
*Odds ratio: 1.9 (0.88–4.14); p-value = 0.101†Odds ratio: 1.5 (1.03–2.26); p-value = 0.035‡Disease control rate (DCR): includes objective response and stable disease
Afatinib
Methotrexate50
40
30
20
10
0ORR DCR‡
*
†
Per
cen
tag
e o
f p
atie
nts
Overall tumour response
60
Overall survival
Time (months)
Est
ima
ted
OS
pro
bab
ilit
y
No. of patientsAfatinib 322 255 172 89 53 28 14 6 1 0MTX 161 115 76 48 29 16 9 7 3 0
Afatinib(n=322)
MTX(n=161)
OS event, n (%) 237 (73.6) 121 (75.2)Median OS (months) 6.8 6.0HR (95% CI) 0.96 (0.77–1.19)Log-rank test p-value 0.700
0
0.2
0.4
0.6
0.8
1.0
0 3 9 15 21 24 2718126
Time to deterioration of pre-specified patient-reported outcomes*
*Assessed using European Organization for Research and Treatment of Cancer (EORTC) questionnaire QLQ-C30 and Head and Neck cancer-specific module (QLQ-H&N35) for pain (composite of items 31–34) and swallowing (composite of items 35–38).†Based on log-rank test.
Global health status
Est
imat
ed p
rob
abili
ty
Time (months)
0
0.2
0.4
0.6
0.8
1.0
0 151413121110987654321
Afatinib
Methotrexate
Pain Swallowing
Est
imat
ed p
rob
abili
ty
Time (months)
0
0.2
0.4
0.6
0.8
1.0
0 16 17 18151413121110987654321
Est
imat
ed p
rob
abili
ty
Time (months)
0
0.2
0.4
0.6
0.8
1.0
0 16 17 18 19 20 21151413121110987654321
HR (95% CI); p-value† 0.74 (0.56–0.97); 0.027
HR (95% CI); p-value† 0.67 (0.50–0.89); 0.004
HR (95% CI); p-value† 0.73 (0.55–0.96); 0.022
Molecular Phenotyping
COMPREHENSIVE GENOMIC CHARACTERIZATION OF SQUAMOUS CELL CARCINOMA OF THE HEAD AND NECK
Neil Hayes, MD, MPHUNC Chapel HillLineberger Comprehensive Cancer Center
ASCO6/3/2013
40
Significantly mutated genes in HNSCC by whole exome sequencing
Analysis – Juok Cho, Peter Hammerman, Carrie Sougnez
CDKN2A 17%FAT1 naTP53 44%CASP8 naJUB naPIK3CA 6%NOTCH1 14%MLL2 7%NSD1 6%HLA-A naTGFBR2 naHRAS 8%EPHA2 naRB1 naNFE2L2 5%KEAP1 naB2M naRAC1 na
COSMIC
MS Lawrence et al. Nature 517, 576-582 (2015) doi:10.1038/nature14129
Deregulation of signalling pathways and transcription factors.
Cancer Immunotherapy
Surgery
Removal of the tumor
Radiotherapy
Energy delivery to specific anatomical site
ImmunotherapyHarnessing the body’s own immune system to
fight cancer
Chemotherapy
Drugs designed to exploit cancer mutations
Pursuing Immunotherapy in Head and Neck Cancer
KEYNOTE-012 – Study Design• Multi-center, non-randomized Phase Ib HNSCC expansion
cohort• Multi-cohort trial* HNSCC cohort
*Additional cohorts included: Bladder Cancer, Triple Negative Breast Cancer, Gastric Cancer
• Presented by: Tanguy Seiwert
Efficacy: Waterfall Plot*
• Presented by: Tanguy Seiwert
51% (26/51) of patients had decreased tumor burden
HPV (+)HPV (-)
Subjects–100
–80
–60
–40
–20
0
20
40
60
80
100
Ch
ang
e F
rom
Bas
elin
e, %
Best percent change from baseline in target lesions (site assessment) delineated by HPV status
*as of May 23, 2014; Includes only patients with RECIST measurable lesions at baseline and at least 1 follow-up scan (n=51)
Best Overall Response*
• Presented by: Tanguy Seiwert
56 pts evaluable for
Response
Total Head/neck
N=56†HPV (+)
N=20
HPV (-)
N=36§
Response Evaluation n (%) 95% CI† n (%) 95% CI† n (%) 95% CI†
Complete Response 1 (1.8) (0.0, 9.6) 1 (5.0) (0.1, 24.9) 0 (0.0) (0.0, 9.7)
Partial Response 10 (17.9) (8.9, 30.4) 3 (15.0) (3.2, 37.9) 7 (19.4) (8.2, 36.0)
Best Overall Response (Complete + Partial)‡
11 (19.6)
(10.2, 32.4)
4 (20.0) (5.7, 43.7)
7 (19.4) (8.2, 36.0)
Stable Disease 16 (28.6) (17.3, 42.2) 8 (40.0) (19.1, 63.9) 8 (22.2) (10.1, 39.2)
Progressive Disease 25 (44.6) (31.3, 58.5) 7 (35.0) (15.4, 59.2) 18 (50.0) (32.9, 67.1)
No Assessment 4 (7.1) (2.0, 17.3) 1 (5.0) (0.1, 24.9) 3 (8.3) (1.8, 22.5)Based on RECIST 1.1 Per site assessment; includes confirmed and unconfirmed responses†61 patients eligible for treatment; 60 patients dosed; 56 patients eligible for pre-defined full analysis set. ‡A single patient with PD followed by PR on treatment was classified as PR.§Includes 2 patients for whom HPV data unavailable.† Based on binomial exact confidence interval method.
• PD-L1 expression correlates with Response• Using a Youden-Index derived, preliminary PD-L1 cut point:
Above cutpoint: 45.5% (5/11) RR Below cutpoint: 11.4% (5/44) RR
*as of May 23, 2014
Time on treatment and disposition*
• Presented by: Tanguy Seiwert
Swimmer plot of all patients who experienced CR or PR. 8 additional patients had SD >6 months, of which 7/8 remain on treatment.
0 4 8 12 16 20 24 28 32 36 40 44 48Treatment Exposure, weeks
Su
bje
cts
Complete ResponsePartial ResponseTreatment Ongoing
*as of May 23, 2014
Toll-like Receptor 8 (TLR8) Pathway is Important in Human Immune Responses
• Activation induces potent Th1 immune response • Expressed on myeloid dendritic cells (CD11c+), monocytes
(CD14+), and natural killer cells (CD56+) in humans• Induces significant IL-12 production in humans• Can be activated by small molecule agonists
• 58
A Randomized, Double-Blind, Placebo-Controlled Study of Chemotherapy Plus Cetuximab in
Combination with VTX-2337 in Patients with Recurrent or Metastatic Squamous Cell Carcinoma
of the Head and Neck
Active8
Study Schema
175 patients with locally advanced or metastatic head/neck cancerPrimary endpoint: progression-free survival
Mutation frequency among cancers of different histotypes
Mutational screen of 305 HNSCC exome sequences deposited in TCGA
HNSCC tumors express missense mutations
Mutational screen of 305 HNSCC exome sequences deposited in TCGA
HNSCC tumors express missense mutations
HNSCC pt
Project: Exome-guided neoantigen discovery in HNSCC
In office/clinic1) Tumor: FNA, core, or excision biopsy2) <1 ml PBL
Bioinformatics:Reassembly, alignment, QC
mutation calling
Confirm SNVs in expressed genes (RNAseq)
gDNA & mRNA
Whole Exome Sequencingsalvage surgery
expand TIL with ‘antigenic mutanome’ peptides
Establish PDX model & perform ACT with ‘improved” TIL
Test peptides for recognition by autologous T cells w ELISPOT
(use prediction algorithm as needed)
Immune monitoring of checkpoint blockade trials
“antigenic mutanome”
CONCLUSIONS• EGFR inhibitors still the only proven
“targeted therapy” in HNSCC• HPV is a recognized prognostic marker
– Other stratification factors needed• Immunotherapy extremely promising
– Phase 3 trials underway in R/M disease likely to complete accrual this year
– PDL1 being explored as predictive biomarker