Your test will be FRIDAY, January 11 and consists of 11 MC, 6 practicum questions and 1 BCR. This study guide is due Thursday, January 10 and will count as a grade.

CHROMATOGRAPHY:

1. Chromatography is a lab technique used to separate

Paper chromatography2. Paper chromatography uses a mobile phase

and a stationary phase .

3. Paper chromatography is used to separate dyes/pigments. In paper

chromatography, inks separate according to their and

______________________

4. In the diagram to the right:a. Which ink color is most soluble?

b. Which ink color is least soluble?

5. We used paper chromatography in the forensics unit to determine

which in Sligo Town processed the salad contaminated

with .

Gel Electrophoresis:

6. The process of gel electrophoresis is used to separates molecules by their

and

7. Electrophoresis is a process that uses to pull molecules from

one place to another.

8. Smaller molecules will move from the wells.

9. Larger molecules will move from the wells.

10. charged molecules will move towards the positive (red) electrode.

Name: _____________________

black

pink

blue

WORD BANKWater or alcohol solubility molecules size blue

black paper factory Salmonella

WORD BANKElectricity check further charge very little away

positively size factory negatively

11. charged molecules will move toward the negative (black) electrode,

12. We used gel electrophoresis chromatography in the forensics unit to ___________ our results

from the paper chromatography lab to determine which ___________

processed the salad contaminated with Salmonella.

13. Look at the chromatography results to the right. a. Which molecule/s (letters) were negatively charged? b. Which molecules (letters) were positively charged?_______

14. You should always _r____________ __the_____ __e___________________ make sure your results are correct.

DNA Fingerprinting (Gel Electrophoresis)

1 2 3 4 5 6

15. In the DNA fingerprint result to the left, which two wells have matching

DNA samples? Wells ____ and ________.

16. We used DNA fingerprinting in the forensics unit to determine which

__________DNA sample matched the DNA sample from the contaminated

salad package to determine which farm was produced the contaminated

salad green.

Urinalysis:

17. The kidneys function to filter out and excess to create the waste product known as urine.

18. Normal urine should test between 4.6 and 8.0 on the pH scale. Identify as acid/base/neutral:

8.0

7.0

4.6

19 . Protein in the urine makes it turn cloudy and can indicate _______________ ________________.

+

(-) electrode

(+) electrode

A

BC

DE

wells

WORD BANKAcid base kidney disease neutral water diabetes

toxins red

20. Glucose in the urine is tested using Benedicts solution and turns brick color and can

indicate what kind of health problems for the person?

Use the following table to answer question 20.

Campylobacter jejuni

S.aureus V. parahaemolyticus

SypmtomsNausea X X xVomiting X X x

Fever XDiarrhea X X x

Chills xCramps x x x

Bacteria in Stool x x

21. Which bacteria would cause nausea, vomiting, fever and diarrhea?

Microarray LAB TECHNIQUE

Red blood cells carry oxygen and Neurons transmit messages carbon dioxide around the body. throughout the body.

22. Different types of cells (like red blood cells and neurons) look different and carry out different

functions because different ___________ are expressed or turned on in different cells.

red blood cells

neuron

+ -

control slide from microarray lab

WORD BANKLook like genes Salmonella gene

23. The purpose of a positive control is to show what a positive results should _________________ to use as a comparison.

24. We used the microarray lab technique in the forensics unit to determine which ingredient in the

contaminated salad was tested positive for the __________________________.

BACTERIA STREAKING25. Bacteria are - celled organisms that reproduce

26. This type of reproduction results in bacteria that is an

of the bacteria it was

produced from.

27. We used bacteria streaking in the forensics unit to determine

which __________ farm is responsible for contaminating the

spinach farm with animal waste containing salmonella transferred

by ________________.

Use the following diagram to answer questions 28 and 29.

28. How does the animal waste from the pig farm

effect the lettuce farm? The runoff from the pig farm

runs to the ____________, which is used to water

the lettuce farm.

29 How does the fence affect the runoff from

the cattle farm? The cattle farm is

surrounded by a __________ and remote

warning system that warns when animal

waste is added to runoff and may

contaminate surrounding farms.

bacteria streaking

WORD BANKAnimal Asexual pond runoff fence Exact copy

uni

Performance Assessment

1) How would you test the pH of a liquid? You would test the pH of a liquid using either pH paper or

a SPARKS probe. With a SPARKS ___________ you would place the probe into the sample and

wait for the digital readout to stop _____________________.

2) Describe a urine sample that contains protein. A urine sample that contains protein would have a

_____________appearance.

3) You have just completed an investigation to check for the presence of glucose in a urine sample.

A positive result would show a ______________________ color.

4) Look at the picture below. Which PATTERN contains negatively charged molecules?

Red Electrode (+)

Black Electrode (-)

WORD BANKCloudy brick red probe changing

Use this graphic organizer to help you formulate your response to BCR prompt 2:

BCR #2: Salmonella Salami

Salami (also spelled “salame”) is a type of dried meat made of sausage, wheat, salt, cornstarch, and several spices, including black pepper. Recently, an outbreak of food poisoning caused by bacteria called Salmonella was traced to contaminated salami. After figuring out the brand of salami that was contaminated, how would you go about identifying the specific ingredient(s) that carried the bacteria?

Topic Sentence:

Evidence piece 1: The process used to identify the contaminated ingredient(s)

Evidence piece 2: How the process would identify the contaminated ingredient(s)

Evidence piece 3: The next steps to be done after identifying the contaminated ingredient(s)

Concluding Sentence: