Supporting Fig. 3. HMPL-004 pre-treatment does not prevent the development of chronic DSS colitis. C57BL/6 mice were given four cycles of DSS drinking water. Mice were orally gavaged daily with 300 mg/kg HMPL-004 or Methyl Cellulose (MC) starting 1 week prior to DSS treatment. Mice were sacrificed on day 29 and assessed for intestinal inflammation. (A) Body weights as percentage of the initial weight at day 1 during the course of colitis (diamonds: MC, squares: HMPL-004, means are shown, n=5 for MC, n=5 for HMPL-004). (B) Colon length of HMPL-004 and MC treated mice at the day of sacrifice. (C) Cell counts for MLN and LP of HMPL-004 and MC treated mice. (D) Colitis scores in cecum, and colon of HMPL-004 or MC treated mice. (E) Mononuclear cells from MLN and LP of HMPL-004 or MC treated mice were cultured in the presence of anti-CD3 and anti-CD28 Abs. IFN- and IL-17 secretion in the supernatant was measured by ELISA. Statistical significance was determined by Student’s t-test.