Web Snap Bean Virus

8/7/2019 Web Snap Bean Virus

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Status of VirusStatus of Virusand Virusand Virus--likelike

Problems onProblems on

Processing BeansProcessing Beansin Wisconsin During 2000in Wisconsin During 2000

SYAIFUL BAKHRI

BIOLOGY ICP 08UNIVERSITAS NEGERI

MAKASSAR


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Description of FieldDescription of FieldSituation in 2000Situation in 2000

Problematic fields near Belgium and Cambria,Problematic fields near Belgium and Cambria,

WisconsinWisconsin

High incidence of plants with virusHigh incidence of plants with virus--likelike

symptomssymptoms

Randomly distributed symptoms in the fieldRandomly distributed symptoms in the field

Many fields exhibiting these symptoms wereMany fields exhibiting these symptoms weredestroyed prior to harvestdestroyed prior to harvest


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Description of FieldDescription of FieldSituation in 2000Situation in 2000

Symptoms observed in the field:Symptoms observed in the field:

Pod distortionPod distortion

Affected pods had a dark brown discolorationAffected pods had a dark brown discoloration

internallyinternally

Leaf distortionLeaf distortion Mottling and mosaic patternsMottling and mosaic patterns


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Symptoms observed at CambriaSymptoms observed at Cambria

Leaf mottling and mosaicLeaf mottling and mosaic

Internal pod discolorationInternal pod discoloration

Pod necrosisPod necrosis


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Leaf mosaic and

necrosis, Cambria


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Leaf mosaicat Cambria


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Rugosity at

Cambria


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Leaf mosaic at

Cambria


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Pod necrosis at

Cambria


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Deformed pods

from Cambria


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Symptoms observed at BelgiumSymptoms observed at Belgium

Leaf mottling and mosaicLeaf mottling and mosaic

Internal pod discolorationInternal pod discoloration

Pod necrosisPod necrosis


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Mottling on leaves

at Belgium


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Leaf mottling at

Belgium


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Rugosity and

mottling at Belgium


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Leaf rugosity

at Belgium


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Pod necrosis

at Belgium


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Pod necrosis at Belgium


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Virus Assays Conducted forVirus Assays Conducted for

Belgium samplesBelgium samples Soybean mosaic virusSoybean mosaic virus

(SMV)(SMV)

Alfalfa mosaic virusAlfalfa mosaic virus(AMV)(AMV)

Clover yellow vein virusClover yellow vein virus

(CYVV)(CYVV)--conducted by Dr.conducted by Dr.

Robert Gilbertson, Univ. of CARobert Gilbertson, Univ. of CA--DavisDavis

Bean yellow mosaicBean yellow mosaic

(BYMV)(BYMV)

Tobacco streak virusTobacco streak virus(TSV)(TSV)

Bean common mosaicBean common mosaic

virus (BCMV)virus (BCMV)

Bean pod mottle virusBean pod mottle virus(BPMV)(BPMV)


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Virus Assays Conducted forVirus Assays Conducted for

Cambria samplesCambria samples Alfalfa mosaic virusAlfalfa mosaic virus

(AMV)(AMV)

Bean yellow mosaic virusBean yellow mosaic virus

(BYMV)(BYMV)

Tobacco streak virusTobacco streak virus

(TSV)(TSV)

Node discoloration atNode discoloration at

CambriaCambria


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Viruses Detected in SymptomaticViruses Detected in Symptomatic

Snap BeansSnap Beans

Viruses AssayedViruses Assayed

AMVAMV BCMVBCMV BYVVBYVV CYVVCYVV TSVTSV

% samples positive% samples positive

BelgiumBelgium 8585 1515 88 100100 1515

CambriaCambria 6767 NANA 00 NANA 00


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Greenhouse ExperimentsGreenhouse Experiments

Inoculum from field infected snap beans causedInoculum from field infected snap beans causedsimilar symptoms on inoculated plantssimilar symptoms on inoculated plants

Pods provided the best source for virus detectionPods provided the best source for virus detectionand transmissionand transmission

Symptoms expressed confirmed by ELISA assaySymptoms expressed confirmed by ELISA assayfor AMVfor AMV


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Transmission of StyletTransmission of Stylet--Borne SnapBorne Snap

Bean VirusesBean Viruses

Aphid transmitted

Alfalfa mosaicAlfalfa mosaicvirus (AMV)virus (AMV)

Aphid and seed transmittedAphid and seed transmitted

Clover yellow veinClover yellow vein

virus (CYVV)virus (CYVV)

Bean yellow mosaicBean yellow mosaicvirus (BYMV)virus (BYMV)

Aphid transmittedAphid transmitted

Center for IPM; NC State



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Transmission of StyletTransmission of Stylet--Borne SnapBorne Snap

Bean VirusesBean Viruses

Bean commonBean common

mosaic virusmosaic virus

(BCMV)(BCMV)

Tobacco streak virusTobacco streak virus

(TSV)(TSV)Thrips transmittedThrips transmitted

Aphid transmittedAphid transmitted


http://newcrop.hort.purdue.edu/rhodcv/

hort410/peas/beanfl.jpg

Seed and pollenSeed and pollen

transmittedtransmitted


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Snap Bean Virus InoculumSnap Bean Virus Inoculum

Sources and Insect TransmissionSources and Insect Transmission

AphidAphid

OtherOther

LegumesLegumes

SeedSeed

AMV, BCMV,AMV, BCMV,

CYVV, BYMVCYVV, BYMV

AMV, BCMV, TSVAMV, BCMV, TSV

AMV, CYVVAMV, CYVV

BYMV, TSVBYMV, TSV


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Typical Symptoms for Detected VirusesTypical Symptoms for Detected Viruses

VirusVirus Leaf Leaf NecrosisNecrosis PodPod

AMVAMV DeformedDeformed MildMild MalformedMalformed

CYVVCYVV Yellow mosaicYellow mosaic ApicalApical Severe malformation; wallSevere malformation; wall

discoloration?discoloration?

BYMVBYMV Yellow mosaicYellow mosaic ApicalApical Slight malformationSlight malformation

BCMVBCMV Green mosaicGreen mosaic SevereSevere Wall discolorationWall discoloration

TSVTSV Dark veinsDark veins Red nodesRed nodes Malformed , lesionsMalformed , lesions


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ConclusionsConclusions Alfalfa mosaic virus (AMV), clover yellow vein (CYVV),Alfalfa mosaic virus (AMV), clover yellow vein (CYVV),

bean common mosaic virus (BCMV), and tobacco streak virusbean common mosaic virus (BCMV), and tobacco streak virus

(TSV) are associated with symptoms observed in 2000.(TSV) are associated with symptoms observed in 2000.

Although AMV was commonly detected, field symptoms wereAlthough AMV was commonly detected, field symptoms weremore closely associated with symptoms characteristic of clovermore closely associated with symptoms characteristic of clover

yellow vein (CYVV) and bean common mosaic virusyellow vein (CYVV) and bean common mosaic virus

(BCMV).(BCMV).

An aphid transmitted complex of viruses are present and meritAn aphid transmitted complex of viruses are present and meritfurther investigation.further investigation.


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Proposed Further ResearchProposed Further Research

Field survey of snap beans and other legumes forField survey of snap beans and other legumes for

types and frequency of viruses.types and frequency of viruses.

Determine whether viruses in snap beans areDetermine whether viruses in snap beans are

common to other legumes.common to other legumes.


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Proposed Further ResearchProposed Further Research

Evaluate snap beans cultivars and germplasm forEvaluate snap beans cultivars and germplasm for

reaction to AMV and CYVV.reaction to AMV and CYVV.

Determine seed transmission ofDetermine seed transmission of

detected viruses.detected viruses.

Determine if viruses are a component of theDetermine if viruses are a component of the

Disease X symptoms in snap beans.Disease X symptoms in snap beans.

Web Snap Bean Virus

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