Analyses for mar99 The treatment and replication names that were found in the data set are listed below. If they do not match the actual treatments and replications that were measured, then terminate R now. If the design is balanced and the missing replications correspond to 0 counts, assign TRUE to the input variable Balanced. Otherwise, ./mar99.data must have entries added with 0 species counts for the missing replications and/or treatments. Replication Names Station22 1 2 3 Station3 1 2 3 Station9 1 2 3 Analysis of Counts at the Genus.Species level The following taxa names had zero counts in all replications and will be removed from the analysis: [1] "Achnanthes hauckiana" [2] "Actinastrum_aciculare" [3] "Actinastrum_hantzschii" [4] "Amphora ovalis v. affinis" [5] "Anabaenopsis_circularis" [6] "Aphanothece_nidulans" [7] "Asterionella formosa" [8] "Aulacoseira distans" [9] "Caloneis ventricosa" [10] "Ceratium_brachyceros" [11] "Ceratium_hirundinella" [12] "cf. Gloeobotrys sp." [13] "Chaetoceros_muelleri" [14] "Chloroflagellates_>10um" [15] "Cholorogonium_spp." [16] "Chroococcus_minimus" [17] "Chroococcus_minutus" [18] "Closterium_aciculare" [19] "Closterium_gracile" [20] "Closterium_sp.1" [21] "Closterium_venus" [22] "Coccoid chrysophyta 5-10um" [23] "Cocconeis_pediculus" [24] "Coelastrum_microporum" [25] "Coelastrum_sphaericum" [26] "Coenocystis_sp.1" [27] "Cosmarium_angulosum" [28] "Cosmarium bireme" [29] "Cosmarium_botrytis" [30] "Cosmarium_granatum" [31] "Cosmarium_tenue" [32] "Crucigenia_crucifera" [33] "Crucigenia_fenestrata" [34] "Crucigenia_rectangularis" [35] "Cryptomonas_curvata" [36] "Cryptomonas_marisonii" [37] "Cyclotella pseudostillegera" [38] "Cyclotella stelligera"

[39] "Dictyosphaerium_ehrenbergainium" [40] "Dictyosphaerium_pulchellum" [41] "Dinobryon bavaricum" [42] "Dinophyceae" [43] "Elakatothrix_viridis" [44] "Elakotothrix_gelatinosa" [45] "Fragilaria construens" [46] "Fragilaria leptostauron" [47] "Fragilaria vaucheriae" [48] "Franceia_armata" [49] "Franceia_ovalis" [50] "Gomphonema olivaceum" [51] "Gomphonema parvulum" [52] "Gymnodium_ordinatum" [53] "Keratococcus_rhaphidioides" [54] "Lagerheimia_ciliata" [55] "Lagerheimia_citriformis" [56] "Lagerheimia_genevensis" [57] "Lagerheimia_longiseta" [58] "Lagerheimia_quadriseta" [59] "Lagerheimia_subsalsa" [60] "Melrosira varians" [61] "Merismopedia_tenuissima" [62] "Navicula capitata" [63] "Navicula citrus" [64] "Navicula gastrum" [65] "Navicula medioconvexa" [66] "Navicula menisculus" [67] "Navicula rhyncocephala var. germanii" [68] "Navicula salarinum var. intermedia" [69] "Navicula secreta v. apiculata" [70] "Navicula teneroides" [71] "Navicula tripunctata v. schizonemoides" [72] "Nephrocytium_agardhianum" [73] "Nitzschia amphibia" [74] "Nitzschia apiculata" [75] "Nitzschia flexiodes" [76] "Nitzschia frustulum v. perminuta" [77] "Nitzschia hungarica" [78] "Nitzschia intermedia" [79] "Nitzschia lacuum" [80] "Nitzschia levidensis var. victoriae" [81] "Nitzschia palea" [82] "Nitzschia palea-debilis" [83] "Nitzschia siliqua" [84] "Nitzschia sp.1" [85] "Nitzschia subacicularis A153" [86] "Nitzschia supralitorea" [87] "Oedogonium_spp." [88] "Oocystis_parva" [89] "Oscillatoria_limnectica" [90] "Oscillatoria_rubescens" [91] "Oscillatoria_tenuis" [92] "Pediastrum_simplex" [93] "Pediastrum_tetras" [94] "Peridinopsis_balticum" [95] "Peridinopsis_quadridens"

[96] "Phacus_cf._orbicularis" [97] "Phacus_cf._tortus" [98] "Phacus_nordstedtii" [99] "Phacus_rudicula" [100] "Phormidium_frigidum" [101] "Pleurosigma salinarium" [102] "Raphidiopsis_indica" [103] "Raphidiopsis_sp.1" [104] "Rhizoselenia sp.1" [105] "Scenedesmus_acuminatus" [106] "Scenedesmus_brevispina" [107] "Scenedesmus_intermedius" [108] "Scenedesmus_obtusus" [109] "Scenedesmus_opoliensis" [110] "Scenedesmus_spinosis" [111] "Staurastrum_chaetoceras" [112] "Stephanodiscus niagarae" [113] "Strombomonopsis_cf._acuminata" [114] "Surirella angusta" [115] "Surirella minuta" [116] "Synedra delicatissima" [117] "Synedra planktonica" [118] "Synedra tenera" [119] "Tetraedron_regulare" [120] "Tetraedron_triangulare" [121] "Tetrastrum_staurogeniaeforme" [122] "Thalassiosira pseudonana" [123] "Thalassiosira sp.1" [124] "Trachelomonas_volvocina" [125] "Treubaria_schmidlei" [126] "Treubaria_setigera" ________________________________________ Analysis for Genus.Species Achnanthes minutissima Multiple comparison for Achnanthes minutissima Means Mean!= Station22 0 Station9 0 Station3 32.2 Station22,Station9 ________________________________________ Analysis for Genus.Species Actinocyclus normanii Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 1.3350 1.3350 0.6079 0.4791 Residuals 4 8.7843 2.1961 Confidence Intervals for Means Lower Upper Station22 -8.591883 29.591883 Station9 -2.879978 5.879978

Individual Dispersion Estimates Station22 Station3 Station9 Mean 10.50000 NA 1.50 Variance 128.25000 NA 6.75 Dispersion 12.21429 NA 4.50 Multiple comparison for Levene’s test: Genus.Species Actinocyclus normanii -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 1.811926 A Station22 2 2.755335 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 64.188 Station 1 22.779 4 41.409 2.7264 0.1740 Over(under)-dispersion parameter = 8.35509 Multiple comparison for Genus.Species Actinocyclus normanii -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 1.500001 A Station22 2 10.500000 A Treaments Station3 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01

Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(0.3856), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 7.6442 Station 1 1.7415 4 5.9027 2.5745 0.1839 Dispersion parameter For Negative Binomial Model = 0.67645 Multiple comparison for Genus.Species Actinocyclus normanii -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station9 1 1.5 A Station22 2 10.5 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 0.285 (-81%) 0.165 (-89%) NA (NA%) Station22 6.237 (-40.6%) 5.46 (-48%) 3.801 (-63.8%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 5.544 (-47.2%) 4.83 (-54%) 3.2865 (-68.7%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 1.3441, df = 1, p-value = 0.2463 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 0 A

Station22 2 9 A ________________________________________ Analysis for Genus.Species Anabaena_flas-quae Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 27.2032 13.6016 7.553 0.02297 * Residuals 6 10.8050 1.8008 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 -1.019913 34.01991 Station3 108.351030 334.51564 Station9 -34.559740 70.55974 Individual Dispersion Estimates Station22 Station3 Station9 Mean 16.500000 221.43333 18 Variance 108.000000 4499.34333 972 Dispersion 6.545455 20.31918 54 Multiple comparison for Levene’s test: Genus.Species Anabaena_flas-quae -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 2.101964 B Station3 2 3.461104 B Station9 3 6.276698 2 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 1114.74 Station 2 936.55 6 178.19 17.404 0.003178 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 26.90549 Multiple comparison for Genus.Species Anabaena_flas-quae -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 16.50000 B Station9 2 18.00007 B Station3 3 221.43333 2 A

Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 106.288 (-52%) 87.6876 (-60.4%) 51.594 (-76.7%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 88.1305 (-60.2%) 71.523 (-67.7%) 39.1937 (-82.3%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(0.6584), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 19.7037 Station 2 8.7596 6 10.9440 5.9269 0.03795 * --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Dispersion parameter For Negative Binomial Model = 0.73898 Multiple comparison for Genus.Species Anabaena_flas-quae -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 16.5000 B Station9 2 18.0000 B Station3 3 221.4333 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 10.7415 (-34.9%) 9.636 (-41.6%) 7.161 (-56.6%) Station9 11.934 (-33.7%) 10.764 (-40.2%) 8.1 (-55%)

Station3 197.5185 (-10.8%) 191.9827 (-13.3%) 177.811 (-19.7%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 9.7845 (-40.7%) 8.745 (-47%) 6.4185 (-61.1%) Station9 10.944 (-39.2%) 9.828 (-45.4%) 7.326 (-59.3%) Station3 193.3113 (-12.7%) 187.9969 (-15.1%) 173.8252 (-21.5%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 5.6949, df = 2, p-value = 0.05799 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 0.0 A Station9 2 22.5 A Station3 3 245.7 A ________________________________________ Analysis for Genus.Species Anabaena_wisconsinese Multiple comparison for Anabaena_wisconsinese Means Mean!= Station22 0 Station9 0 Station3 27.3 Station22,Station9 ________________________________________ Analysis for Genus.Species Ankistrodesmus_falcatus_var._1 Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 66.815 33.407 4.1275 0.07457 . Residuals 6 48.562 8.094 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 61.633656 147.69968 Station3 56.267502 819.13250 Station9 -1.393108 49.99311 Individual Dispersion Estimates

Station22 Station3 Station9 Mean 104.666667 437.7000 24.300000 Variance 651.573333 51191.1900 232.270000 Dispersion 6.225223 116.9550 9.558436 Multiple comparison for Levene’s test: Genus.Species Ankistrodesmus_falcatus_var._1 -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 1.950163 B Station9 2 2.288348 B Station3 3 7.891734 2 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 1799.77 Station 2 1537.25 6 262.52 17.439 0.003162 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 44.07548 Multiple comparison for Genus.Species Ankistrodesmus_falcatus_var._1 -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 24.30073 B Station22 2 104.66667 B Station3 3 437.70306 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 21.666 (-79.3%) 13.0833 (-87.5%) NA (NA%) Station3 225.8548 (-48.4%) 189.9631 (-56.6%) 118.6175 (-72.9%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%)

Station3 192.1516 (-56.1%) 159.7616 (-63.5%) 94.9816 (-78.3%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(7.4354), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 84.148 Station 2 74.789 6 9.359 24.357 0.001319 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Dispersion parameter For Negative Binomial Model = 1.53528 Multiple comparison for Genus.Species Ankistrodesmus_falcatus_var._1 -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station9 1 24.3000 C Station22 2 104.6667 1 B Station3 3 437.7000 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 14.5071 (-40.3%) 12.7332 (-47.6%) 8.8938 (-63.4%) Station22 82.268 (-21.4%) 77.4533 (-26%) 65.626 (-37.3%) Station3 389.553 (-11%) 378.1728 (-13.6%) 349.7223 (-20.1%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 12.9276 (-46.8%) 11.2509 (-53.7%) 7.7031 (-68.3%) Station22 78.3953 (-25.1%) 73.6853 (-29.6%) 62.3813 (-40.4%) Station3 380.799 (-13%) 369.8565 (-15.5%) 341.406 (-22%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 7.2, df = 2, p-value = 0.02732

Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 20.1 A Station22 2 118.8 A Station3 3 386.4 A ________________________________________ Analysis for Genus.Species Ankistrodesmus_spiralis Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 0.0788 0.0788 0.0233 0.886 Residuals 4 13.4953 3.3738 Confidence Intervals for Means Lower Upper Station22 -2.265298 92.2653 Station3 28.843610 143.8897 Individual Dispersion Estimates Station22 Station3 Station9 Mean 45.00000 86.36667 NA Variance 786.04000 1164.24333 NA Dispersion 17.46756 13.48024 NA Multiple comparison for Levene’s test: Genus.Species Ankistrodesmus_spiralis -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station3 1 2.801394 A Station22 2 3.030560 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 104.343 Station 1 39.751 4 64.592 2.569 0.1842 Over(under)-dispersion parameter = 15.4736 Multiple comparison for Genus.Species Ankistrodesmus_spiralis -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 45.00000 A Station3 2 86.36667 A Treaments Station9 had 0 abundance for all replications.

The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 11.475 (-74.5%) 7.65 (-83%) 1.62 (-96.4%) Station3 34.5467 (-60%) 27.1191 (-68.6%) 13.4732 (-84.4%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 6.21 (-86.2%) NA (NA%) NA (NA%) Station3 26.6009 (-69.2%) 20.0371 (-76.8%) 8.1185 (-90.6%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(5.6823), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 9.4847 Station 1 3.2632 4 6.2215 2.3387 0.2009 Dispersion parameter For Negative Binomial Model = 1.39528 Multiple comparison for Genus.Species Ankistrodesmus_spiralis -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 45.00000 A Station3 2 86.36667 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 31.59 (-29.8%) 28.845 (-35.9%) 22.635 (-49.7%) Station3 67.0205 (-22.4%) 62.8749 (-27.2%) 52.9428 (-38.7%)

Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 29.295 (-34.9%) 26.73 (-40.6%) 20.79 (-53.8%) Station3 63.7386 (-26.2%) 59.6794 (-30.9%) 50.0927 (-42%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 2.3333, df = 1, p-value = 0.1266 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 40.0 A Station3 2 96.6 A ________________________________________ Analysis for Genus.Species Aulacoseira granulata Multiple comparison for Aulacoseira granulata Means Mean!= Station22 0 Station3 0 Station9 22.5 Station22,Station3 ________________________________________ Analysis for Genus.Species Carteria_spp. Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 13.497 6.748 0.6639 0.549 Residuals 6 60.992 10.165 Confidence Intervals for Means Lower Upper Station22 -11.354930 34.28826 Station3 -30.642690 133.10936 Station9 -8.430138 30.76347 Individual Dispersion Estimates Station22 Station3 Station9 Mean 11.46667 51.23333 11.16667 Variance 183.25333 2358.70333 135.12333 Dispersion 15.98140 46.03845 12.10060

Multiple comparison for Levene’s test: Genus.Species Carteria_spp. -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 2.709673 A Station22 2 3.211883 A Station3 3 5.521880 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 325.13 Station 2 119.69 6 205.44 2.4324 0.1684 Over(under)-dispersion parameter = 24.60284 Multiple comparison for Genus.Species Carteria_spp. -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 11.16676 A Station22 2 11.46695 A Station3 3 51.23361 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 8.9659 (-82.5%) 4.8672 (-90.5%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(3090.150), link: log Response: Count

Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 322.40 Station 2 118.67 6 203.73 2.4295 0.1687 Dispersion parameter For Negative Binomial Model = 24.42207 Multiple comparison for Genus.Species Carteria_spp. -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station9 1 11.16667 A Station22 2 11.46667 A Station3 3 51.23333 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 9.0683 (-82.3%) 4.9696 (-90.3%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 1.1034, df = 2, p-value = 0.576 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 8.0 A Station22 2 10.3 A Station3 3 57.1 A ________________________________________ Analysis for Genus.Species Centritractis sp.1 Multiple comparison for Centritractis sp.1

Means Mean!= Station22 0 Station9 0 Station3 648.5 Station22,Station9 ________________________________________ Analysis for Genus.Species Chlamydomonas_sp.1 Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 112.466 56.233 5.2744 0.04766 * Residuals 6 63.969 10.662 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 11.15188 53.78145 Station3 67.22509 1083.24158 Station9 -14.84789 30.31456 Individual Dispersion Estimates Station22 Station3 Station9 Mean 32.466667 575.2333 7.733333 Variance 159.853333 90803.2433 179.413333 Dispersion 4.923614 157.8546 23.200000 Multiple comparison for Levene’s test: Genus.Species Chlamydomonas_sp.1 -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 1.682813 B Station9 2 4.114133 B Station3 3 10.095676 2 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 3476.4 Station 2 3047.4 6 429.0 24.586 0.001286 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 61.97479 Multiple comparison for Genus.Species Chlamydomonas_sp.1 -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group

Station9 1 7.733354 B Station22 2 32.466667 B Station3 3 575.233333 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 289.3424 (-49.7%) 242.1732 (-57.9%) 147.835 (-74.3%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 244.4742 (-57.5%) 201.3317 (-65%) 116.1971 (-79.8%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(1.0477), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 36.989 Station 2 25.248 6 11.741 11.802 0.008325 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Dispersion parameter For Negative Binomial Model = 1.06963 Multiple comparison for Genus.Species Chlamydomonas_sp.1 -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station9 1 7.733333 B Station22 2 32.466667 B Station3 3 575.233333 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2

alpha 0.1 0.05 0.01 Station9 3.5109 (-54.6%) 2.8536 (-63.1%) 1.6008 (-79.3%) Station22 22.5319 (-30.6%) 20.5189 (-36.8%) 15.9736 (-50.8%) Station3 528.6394 (-8.1%) 517.1348 (-10.1%) 488.3731 (-15.1%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 2.8536 (-63.1%) 2.2659 (-70.7%) 1.1523 (-85.1%) Station22 20.8436 (-35.8%) 18.9605 (-41.6%) 14.6425 (-54.9%) Station3 520.0109 (-9.6%) 509.0815 (-11.5%) 480.3198 (-16.5%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 7.2605, df = 2, p-value = 0.02651 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 0.0 A Station22 2 26.4 A Station3 3 724.5 A ________________________________________ Analysis for Genus.Species Chloroflagellates_3-10um Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 763.48 763.48 176.14 0.0001863 *** Residuals 4 17.34 4.33 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station3 -548.155883 1119.15588 Station9 -6.811251 27.07792 Individual Dispersion Estimates Station22 Station3 Station9 Mean NA 285.5 10.133333 Variance NA 244530.8 101.023333 Dispersion NA 856.5 9.969408 Multiple comparison for Levene’s test: Genus.Species Chloroflagellates_3-10um -- Quasi-Poisson Model

Rank Sorted Means Highest Rank > Group Station9 1 2.436912 B Station3 2 24.997583 1 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 2874.45 Station 1 964.66 4 1909.79 2.2336 0.2093 Over(under)-dispersion parameter = 431.88101 Multiple comparison for Genus.Species Chloroflagellates_3-10um -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 10.13333 A Station3 2 285.50143 A Treaments Station22 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(6057.067), link: log Response: Count

Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 2780.06 Station 1 946.33 4 1833.73 2.2861 0.2051 Dispersion parameter For Negative Binomial Model = 413.94728 Multiple comparison for Genus.Species Chloroflagellates_3-10um -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station9 1 10.13333 A Station3 2 285.50000 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 0.0538, df = 1, p-value = 0.8166 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 0.0 A Station3 2 10.3 A ________________________________________ Analysis for Genus.Species Closterium_acutum Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid

Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 0.96325 0.48163 3.8553 0.0838 . Residuals 6 0.74955 0.12492 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 -2.879978 5.879978 Station3 -5.823956 11.890623 Station9 -4.139935 22.139935 Individual Dispersion Estimates Station22 Station3 Station9 Mean 1.50 3.033333 9.00 Variance 6.75 27.603333 60.75 Dispersion 4.50 9.100000 6.75 Multiple comparison for Levene’s test: Genus.Species Closterium_acutum -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 1.811926 B Station9 2 1.986856 AB Station3 3 2.576647 1 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 62.523 Station 2 20.164 6 42.359 1.4876 0.2988 Over(under)-dispersion parameter = 6.77726 Multiple comparison for Genus.Species Closterium_acutum -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 1.500001 A Station3 2 3.033337 A Station9 3 9.000000 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%)

Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(0.5852), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 12.1910 Station 2 2.4493 6 9.7417 1.1676 0.373 Dispersion parameter For Negative Binomial Model = 1.04884 Multiple comparison for Genus.Species Closterium_acutum -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 1.500000 A Station3 2 3.033333 A Station9 3 9.000000 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 0.114 (-92.4%) NA (NA%) NA (NA%) Station3 0.7674 (-74.7%) 0.5126 (-83.1%) 0.1031 (-96.6%) Station9 4.392 (-51.2%) 3.645 (-59.5%) 2.169 (-75.9%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 0.4125 (-86.4%) NA (NA%) NA (NA%) Station9 3.663 (-59.3%) 2.997 (-66.7%) 1.665 (-81.5%)

Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 2.8176, df = 2, p-value = 0.2444 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 0.0 A Station3 2 0.0 A Station9 3 4.5 A ________________________________________ Analysis for Genus.Species Cocciod Chlorophyta_5-10um Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 39.32 19.66 0.8695 0.466 Residuals 6 135.66 22.61 Confidence Intervals for Means Lower Upper Station22 -7.764205 104.0309 Station3 -84.951872 301.6185 Station9 -38.347925 181.0813 Individual Dispersion Estimates Station22 Station3 Station9 Mean 48.13333 108.3333 71.36667 Variance 1099.37333 13144.8933 4235.34333 Dispersion 22.84017 121.3375 59.34624 Multiple comparison for Levene’s test: Genus.Species Cocciod Chlorophyta_5-10um -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 3.528625 A Station9 2 5.730391 A Station3 3 8.632539 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 567.65

Station 2 72.56 6 495.09 0.5365 0.6104 Over(under)-dispersion parameter = 67.62203 Multiple comparison for Genus.Species Cocciod Chlorophyta_5-10um -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 48.13333 A Station9 2 71.36669 A Station3 3 108.33468 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 11.8085 (-89.1%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(759.3616), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 518.00 Station 2 65.96 6 452.04 0.5439 0.6066 Dispersion parameter For Negative Binomial Model = 60.63778 Multiple comparison for Genus.Species Cocciod Chlorophyta_5-10um -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 48.13333 A Station9 2 71.36667 A Station3 3 108.33333 A Observable Decrease in Means: These values represent observable decreases

for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 14.8417 (-86.3%) 6.0667 (-94.4%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 0.3556, df = 2, p-value = 0.8371 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 40.0 A Station9 2 61.8 A Station3 3 96.6 A ________________________________________ Analysis for Genus.Species Cocciod Chlorophyta_F) Treatment 2 1619.26 809.63 20.474 0.002087 ** Residuals 6 237.26 39.54 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 155.4841 418.3826 Station3 -985.4622 2434.4622 Station9 102.2215 167.6451 Individual Dispersion Estimates Station22 Station3 Station9

Mean 286.93333 724.50 134.933333 Variance 6079.61333 1028804.49 376.503333 Dispersion 21.18824 1420.02 2.790292 Multiple comparison for Levene’s test: Genus.Species Cocciod Chlorophyta_ Group Station9 1 1.249709 B Station22 2 3.476951 B Station3 3 30.751905 2 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 4560.2 Station 2 1445.0 6 3115.3 1.5013 0.2960 Over(under)-dispersion parameter = 481.25144 Multiple comparison for Genus.Species Cocciod Chlorophyta_ Group Station9 1 134.9333 A Station22 2 286.9333 A Station3 3 724.5000 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 66.654 (-90.8%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model

Analysis of Deviance Table Model: Negative Binomial(10183.72), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 4320.2 Station 2 1392.0 6 2928.2 1.5477 0.2871 Dispersion parameter For Negative Binomial Model = 449.68893 Multiple comparison for Genus.Species Cocciod Chlorophyta_ Group Station9 1 134.9333 A Station22 2 286.9333 A Station3 3 724.5000 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 80.4195 (-88.9%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 2.4889, df = 2, p-value = 0.2881 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 140.7 A Station22 2 253.8 A

Station3 3 289.8 A ________________________________________ Analysis for Genus.Species Cocciod Chrysophyta

Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 274.097 Station 1 80.541 4 193.556 2.5228 0.1874 Over(under)-dispersion parameter = 31.92527 Multiple comparison for Genus.Species Coccoid Cyanophyta_ Group Station22 1 21.80000 A Station3 2 70.26689 A Treaments Station9 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 13.2804 (-81.1%) 7.5888 (-89.2%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(2349.199), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 269.30 Station 1 79.07 4 190.23 2.5371 0.1864 Dispersion parameter For Negative Binomial Model = 31.16553 Multiple comparison for Genus.Species Coccoid Cyanophyta_ Group

Station22 1 21.80000 A Station3 2 70.26667 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 13.6317 (-80.6%) 8.0104 (-88.6%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 0.4286, df = 1, p-value = 0.5127 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 19.8 A Station3 2 96.6 A ________________________________________ Analysis for Genus.Species Cosmarium_subcrenatum Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 0.31503 0.31503 0.685 0.4544 Residuals 4 1.83960 0.45990 Confidence Intervals for Means Lower Upper Station22 -2.879978 5.879978 Station9 -1.379978 7.379978 Individual Dispersion Estimates Station22 Station3 Station9 Mean 1.50 NA 3.00 Variance 6.75 NA 6.75

Dispersion 4.50 NA 2.25 Multiple comparison for Levene’s test: Genus.Species Cosmarium_subcrenatum -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 1.353644 A Station22 2 1.811926 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 18.7150 Station 1 1.5291 4 17.1859 0.4533 0.5377 Over(under)-dispersion parameter = 3.37301 Multiple comparison for Genus.Species Cosmarium_subcrenatum -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 1.500001 A Station9 2 3.000000 A Treaments Station3 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table

Model: Negative Binomial(0.6312), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 6.6971 Station 1 0.3441 4 6.3530 0.3992 0.5618 Dispersion parameter For Negative Binomial Model = 0.86197 Multiple comparison for Genus.Species Cosmarium_subcrenatum -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 1.5 A Station9 2 3.0 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 0.1965 (-86.9%) 0.069 (-95.4%) NA (NA%) Station9 0.888 (-70.4%) 0.63 (-79%) 0.21 (-93%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 0.567 (-81.1%) 0.339 (-88.7%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 0.5556, df = 1, p-value = 0.4561 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 0.0 A Station9 2 4.5 A ________________________________________ Analysis for Genus.Species Crucigenia_quadrata

Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 0.63521 0.63521 4.0807 0.1135 Residuals 4 0.62265 0.15566 Confidence Intervals for Means Lower Upper Station22 -20.47985 41.81318 Station9 -26.36780 53.83447 Individual Dispersion Estimates Station22 Station3 Station9 Mean 10.66667 NA 13.73333 Variance 341.33333 NA 565.81333 Dispersion 32.00000 NA 41.20000 Multiple comparison for Levene’s test: Genus.Species Crucigenia_quadrata -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 4.831804 A Station9 2 5.482554 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 161.996 Station 1 1.159 4 160.837 0.0318 0.8672 Over(under)-dispersion parameter = 36.50559 Multiple comparison for Genus.Species Crucigenia_quadrata -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 10.66670 A Station9 2 13.73338 A Treaments Station3 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2

alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(410.9607), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 157.720 Station 1 1.126 4 156.594 0.0317 0.8674 Dispersion parameter For Negative Binomial Model = 35.52895 Multiple comparison for Genus.Species Crucigenia_quadrata -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 10.66667 A Station9 2 13.73333 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test

Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 0.0667, df = 1, p-value = 0.7963 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 0 A Station9 2 0 A ________________________________________ Analysis for Genus.Species Crucigenia_tetrapedia Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 537.14 537.14 69.162 0.001142 ** Residuals 4 31.07 7.77 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 -42.8075 124.4075 Station3 -556.4118 1136.0118 Individual Dispersion Estimates Station22 Station3 Station9 Mean 40.80000 289.8 NA Variance 2459.52000 251952.1 NA Dispersion 60.28235 869.4 NA Multiple comparison for Levene’s test: Genus.Species Crucigenia_tetrapedia -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 6.261861 B Station3 2 25.185128 1 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 2685.29 Station 1 633.72 4 2051.57 1.3673 0.3072

Over(under)-dispersion parameter = 463.46571 Multiple comparison for Genus.Species Crucigenia_tetrapedia -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 40.8000 A Station3 2 289.8015 A Treaments Station9 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(6692.508), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 2599.77 Station 1 620.16 4 1979.61 1.3886 0.3040 Dispersion parameter For Negative Binomial Model = 446.61418 Multiple comparison for Genus.Species Crucigenia_tetrapedia -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 40.8 A Station3 2 289.8 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across

more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 0.0538, df = 1, p-value = 0.8166 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 0.0 A Station3 2 26.4 A ________________________________________ Analysis for Genus.Species Cryptomonas_erosa Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 33.589 16.794 2.4643 0.1655 Residuals 6 40.891 6.815 Confidence Intervals for Means Lower Upper Station22 55.43588 224.6975 Station3 174.07898 894.5210 Station9 147.83454 372.1655 Individual Dispersion Estimates Station22 Station3 Station9 Mean 140.0667 534.30000 260.00000 Variance 2520.0933 45656.01000 4426.68000 Dispersion 17.9921 85.45014 17.02569 Multiple comparison for Levene’s test: Genus.Species Cryptomonas_erosa -- Quasi-Poisson Model

Rank Sorted Means Highest Rank > Group Station9 1 3.128117 A Station22 2 3.200115 A Station3 3 7.261744 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 1036.05 Station 2 776.88 6 259.17 9.6865 0.01322 * --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 40.10108 Multiple comparison for Genus.Species Cryptomonas_erosa -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 140.0667 B Station9 2 260.0000 B Station3 3 534.3008 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 41.5998 (-70.3%) 29.5541 (-78.9%) 9.8047 (-93%) Station9 112.32 (-56.8%) 89.96 (-65.4%) 48.1 (-81.5%) Station3 303.4828 (-43.2%) 262.876 (-50.8%) 176.8536 (-66.9%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 26.6127 (-81%) 15.9676 (-88.6%) NA (NA%) Station9 89.44 (-65.6%) 69.68 (-73.2%) 32.76 (-87.4%) Station3 266.6161 (-50.1%) 228.6807 (-57.2%) 149.6042 (-72%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(12.0593), link: log Response: Count

Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 39.558 Station 2 30.494 6 9.064 11.247 0.009337 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Dispersion parameter For Negative Binomial Model = 1.35567 Multiple comparison for Genus.Species Cryptomonas_erosa -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 140.0667 C Station9 2 260.0000 1 B Station3 3 534.3000 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 115.2749 (-17.7%) 109.6722 (-21.7%) 96.0857 (-31.4%) Station9 225.42 (-13.3%) 217.62 (-16.3%) 197.6 (-24%) Station3 484.0758 (-9.4%) 471.7869 (-11.7%) 441.3318 (-17.4%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 110.9328 (-20.8%) 105.6103 (-24.6%) 92.3039 (-34.1%) Station9 219.44 (-15.6%) 211.64 (-18.6%) 191.88 (-26.2%) Station3 474.9927 (-11.1%) 463.2381 (-13.3%) 432.783 (-19%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 5.6889, df = 2, p-value = 0.05817 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 118.8 A Station9 2 281.4 A Station3 3 627.9 A ________________________________________

Analysis for Genus.Species Cyclotella atomus Multiple comparison for Cyclotella atomus Means Mean!= Station22 0 Station9 0 Station3 3327.23333 Station22,Station9 ________________________________________ Analysis for Genus.Species Cyclotella meneghiniana Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 34.394 17.197 3.294 0.1083 Residuals 6 31.324 5.221 Confidence Intervals for Means Lower Upper Station22 -26.5324209 72.93242 Station3 0.2021750 881.06449 Station9 -29.6957770 60.62911 Individual Dispersion Estimates Station22 Station3 Station9 Mean 23.20000 440.6333 15.46667 Variance 870.24000 68252.0833 717.65333 Dispersion 37.51034 154.8954 46.40000 Multiple comparison for Levene’s test: Genus.Species Cyclotella meneghiniana -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 4.962237 A Station9 2 5.818262 A Station3 3 9.470346 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 2672.66 Station 2 2196.85 6 475.81 13.806 0.005688 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 79.5597

Multiple comparison for Genus.Species Cyclotella meneghiniana -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station9 1 15.46672 A Station22 2 23.20000 A Station3 3 440.63333 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 175.8127 (-60.1%) 137.4776 (-68.8%) 68.2982 (-84.5%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 135.2744 (-69.3%) 101.3457 (-77%) 40.5383 (-90.8%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(2500.901), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 2494.45 Station 2 2065.56 6 428.89 14.413 0.005114 ** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Dispersion parameter For Negative Binomial Model = 71.65758 Multiple comparison for Genus.Species Cyclotella meneghiniana -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station9 1 15.46667 B Station22 2 23.20000 B Station3 3 440.63333 2 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups.

Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 185.5066 (-57.9%) 147.6122 (-66.5%) 77.1108 (-82.5%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station9 NA (NA%) NA (NA%) NA (NA%) Station22 NA (NA%) NA (NA%) NA (NA%) Station3 146.2903 (-66.8%) 112.3615 (-74.5%) 50.2322 (-88.6%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test data: Count ~ Station Kruskal-Wallis chi-squared = 5.8435, df = 2, p-value = 0.05384 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station9 1 0.0 A Station22 2 13.2 A Station3 3 289.8 A ________________________________________ Analysis for Genus.Species Cyclotella ocellata Multiple comparison for Cyclotella ocellata Means Mean!= Station3 0 Station9 0 Station22 18.2 Station3,Station9 ________________________________________ Analysis for Genus.Species Diploneis puella Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 2 8.0767 4.0384 1.0929 0.3938 Residuals 6 22.1702 3.6950 Confidence Intervals for Means Lower Upper Station22 -5.119962 10.45329

Station3 -16.694651 86.96132 Station9 -7.423944 15.15728 Individual Dispersion Estimates Station22 Station3 Station9 Mean 2.666667 35.13333 3.866667 Variance 21.333333 945.12333 44.853333 Dispersion 8.000000 26.90104 11.600000 Multiple comparison for Levene’s test: Genus.Species Diploneis puella -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 2.415902 A Station9 2 2.909131 A Station3 3 4.626162 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 268.84 Station 2 139.56 6 129.27 4.5051 0.06387 . --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 15.48955 Multiple comparison for Genus.Species Diploneis puella -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 2.666669 A Station9 2 3.866673 A Station3 3 35.133333 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 6.8861 (-80.4%) 4.0403 (-88.5%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1

alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) ANOVA Test for Treatment Effects, Negative Binomial Model Analysis of Deviance Table Model: Negative Binomial(1246.061), link: log Response: Count Terms added sequentially (first to last) Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 8 265.64 Station 2 137.96 6 127.69 4.5271 0.06331 . --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Dispersion parameter For Negative Binomial Model = 15.23679 Multiple comparison for Genus.Species Diploneis puella -- Negative Binomial Model Rank Sorted Means Highest Rank > Group Station22 1 2.666667 A Station9 2 3.866667 A Station3 3 35.133333 A Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 7.0267 (-80%) 4.1809 (-88.1%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Negative Binomial Model, Beta = 0.1 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station9 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Kruskal-Wallis Rank Test Kruskal-Wallis rank sum test

data: Count ~ Station Kruskal-Wallis chi-squared = 2.0267, df = 2, p-value = 0.363 Kruskal-Wallis Multiple Comparisons Rank Sorted Medians Highest Rank > Group Station22 1 0.0 A Station9 2 0.0 A Station3 3 48.3 A ________________________________________ Analysis for Genus.Species Entomoneis paludosa Multiple comparison for Entomoneis paludosa Means Mean!= Station22 0 Station9 0 Station3 67.33333 Station22,Station9 ________________________________________ Analysis for Genus.Species Euglena_spp. Levene’s Test for Homogeneity of Dispersion Analysis of Variance Table Response: AbsResid Df Sum Sq Mean Sq F value Pr(>F) Treatment 1 440.37 440.37 27.583 0.006289 ** Residuals 4 63.86 15.97 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Confidence Intervals for Means Lower Upper Station22 -1.379978 7.379978 Station3 -388.683528 1051.350195 Individual Dispersion Estimates Station22 Station3 Station9 Mean 3.00 331.3333 NA Variance 6.75 182408.5433 NA Dispersion 2.25 550.5288 NA Multiple comparison for Levene’s test: Genus.Species Euglena_spp. -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 1.353643 B Station3 2 18.487742 1 A ANOVA Test for Treatment Effects Analysis of Deviance Table Model: quasipoisson, link: log Response: Count Terms added sequentially (first to last)

Df Deviance Resid. Df Resid. Dev F Pr(>F) NULL 5 2382.4 Station 1 1287.7 4 1094.8 4.6942 0.09617 . --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Over(under)-dispersion parameter = 274.31491 Multiple comparison for Genus.Species Euglena_spp. -- Quasi-Poisson Model Rank Sorted Means Highest Rank > Group Station22 1 3.000004 A Station3 2 331.342775 A Treaments Station9 had 0 abundance for all replications. The critical value to compare treatments with 0 abundance to the non-zero treatments is 0.333333333333333 Any treatment mean greater than this critical value is significantly greater than the 0 treatments. Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.2 alpha 0.1 0.05 0.01 Station22 NA (NA%) NA (NA%) NA (NA%) Station3 NA (NA%) NA (NA%) NA (NA%) Observable Decrease in Means: These values represent observable decreases for pairwise comparisons only. They are not applicable for comparisons across more than two groups. Observable Decrease in Means, Quasi-Poisson Model, Beta = 0.1 alpha 0.1