votume 17, Supplement Nucleic Acids Research Restriction …repository.cshl.edu/34901/1/Roberts...

votume 17, Supplement Nucleic Acids Research

Restriction enzymes and their boschizomers

Richard J.Roberts

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

INTRODUCTIONSince the last compilation of restriction enzymes (1), 300 new entries have been addedincluding 12 new specificities. With the growing size of this database and the recognitionthat the most widespread use of the information is as a database for computer programspredicting restriction enzyme cleavage patterns, the new format has been continued. Thisformat is intended to contain the minimal amount of information required by a computerprogram. It should be noted that only enzymes for which the recognition sequence is knownare included. This new list is shown"in the first Table, while an alphabetical listing ofall Type II enzymes is presented in the second Table. A copy of the restriction enzymedata base in its previous format (2), including enzymes of unknown recognition sequence,will be available upon request. It should also be noted that an alternative compilation ofthese enzymes has recently been produced (3).

The database shown in these Tables is available online through the BIONET computerresource. A version corresponding to the printed text is located in the file< ROBERTS > RESTRICT.NAR Several alternative versions are available and aredocumented in < ROBERTS > RESTRICT.DOC. It is also possible to get regular monthlyupdates by electronic mail. Enquiries should be directed to [email protected].

In forming this list, all endonucleases cleaving DNA at a specific sequence have beenconsidered to be restriction enzymes, although in most cases there is no direct geneticevidence for the presence of a restriction-modification system. The endonucleases are namedin accordance with the proposal of Smith and Nathans (4).

ACKNOWLEDGEMENTSSpecial thanks are due to all the individuals who have so kindly communicated theirunpublished results to be included in this compilation. Work from the author's laboratoryis supported by the NSF (DMB8613588) and the NIH (GM40537).

©IRL Press r347

Downloaded from https://academic.oup.com/nar/article-abstract/17/suppl/r347/1218527by Cold Spring Harbor Laboratory useron 08 November 2017

Nucleic Acids Research

Enzyme

EcoMEcoBlEcoDlEcoDXXlEcoElEcoKlEcoRUWl£<x>R124ISrySBISrySJISrySPIStySQl

Enzyme1

AataAcdAcyl

AflU

AflmAham

AM

AtwmApal

ApaU

Recognitionsequence

GAGNNNNNNNGTCATGANNNNNNNNTGCTTTANNNNNNNGTCYTCANNNNNNNATTCGAGNNNNNNNATGCAACNNNNNNGTGCGAANNNNNNNRTCGGAANNNNNNRTCGGAGNNNNNNRTAYGGAGNNNNNNGTRCAACNNNNNNGTRCAACNNNNNNRTAYG

Isoschizomers Recognition2

AhaO.AosUAstWlAsuUlBbiQHgiDlHgiGlHgiHUHinUHinSlNlaSU

Eip4I

Dral

MidOmlOfuNIOxa\

Bspl20EaEl

AmelSnolUbo44lVnel

Sequence

GACGTtCGTtMKACGRtCGYCGRtCGYCGRtCGYCGRtCGYCGRtCGYCGRtCGYCGRtCGYCGRICGYCGRtCGYCGRICGYCGRCGYCGRCGYCCITTAAGCITTAAGAICRYGTTTTtAAATTTtAAAAGICTAGICTAGCTAGCTAGCTCAGNNNtCTGGGGCCIC

I GGGCCCGGGCCCGITGCACGTGCACGITGCACGITGCACGITGCAC

Type I enzymes

Me

Type II enzymes

Me3

site

3(5)

4(5)

site3

2(6) -3(6)3(6) -4(6)

2(6) -3(6)-3(6)

2(6) -4(6)

2(6) -3(6)

Commercial4

source

GMNPRUABGIMNPRUMRN

A

AGN

G

ABGIMNPRUABGIMNPRU

NBGIMNPRU

AGN

Reference

545,546547-551

552553,554555,556

557-560561561562563562564

Reference

56789

1010111212131415161718171920

21-2425,26

27282930

31,3233343527

36,373839

r348



Enzyme1 Isoschizomers Recognition2

SequenceMe3

siteCommercial4

sourceReference

Asul

AsuU

Aval

ApulBac36lBspBUCfiAlCfrSlQH 31QT-231Qr33IC>451CfiA6\Cfr\l\Cfr52\Cfr54lQrNI£co39I£co47nEco\96UEcoTOUGse\Hin5HMjaUNlaDUNnwEllNmuSlNspWNsplMPselPsplSau96lSdyl

AcalAvilfisp82Ifispll9IfirtBICsp45IFsphLsplMlalNspVNspBlNspFlNspllSspU

AcriAqulAspBlAspClAspDlAvri

GtGNCCGGNCCGtGNCCGIGNCCGGNCCGGNCCGtGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGGNCCGtGNCCGGNCCGGNCCGGNCCGIGNCCGGNCCTTtCGAATTCGAATTCGAATTCGAATTCGAATTtCGAATTICGAATTICGAATTICGAATTICGAATTCGAATTCGAATTCGAATTCGAATTICGAACtYCGRGCYCGRGCIYCGRGCYCGRGCYCGRGCYCGRGCYCGRG

4(5)

1(5)

R

AU

P

BGMNR

G

NR

AP

ABGIMNPRU

40414142

43,4443,4443,45

46474747474849505253515154155556575859

60,6162636465

10,6667,68

626948

70,7172,73

7436,37

75597654

60,6877

78,7968

80,8176767682

r349




SequenceMe3

siteCommercial4

sourceReference

AvaU

flrtSIEcoSSlMlNmuMNspUlNspDlNspElNspSM

AfllAspeniAspBUAspcaAspDUBamNjlBme216lfisp 1001flWICaulCUT.OmUEagMlEaAllErplFdilFspMSlGspMHgiBlHgiCaHgiElHgiHmHgiilHspl\MfolMspM

maNmuAHNspDHNspGlNspHENspKlSfrlSinlSinMSinBlSinClSinDlSinUSinFlSinGlSinUlSMITnd

C1YCGRGCYCGRGCYCGRGCYCGRGCIYCGRGCYCGRGCYCGRGCIYCGRGGIGWCCG1GWCCGGWCCGGWCCGGWCCGGWCCGIGWCCGIGWCCGGWCCGGWCCGIGWCCGGWCCGGWCCGtGWCCGtGWCCGIGWCCGIGWCCGIGWCCGGWCCGIGWCCGtGWCCGIGWCCGtGWCCGIGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGIGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCCGGWCC

ABGIMNPR

4(5)

7083767659768485

78,79,86,871788767676

89-9192,93

3394

95,966197

98,9953,100

41101,102

41103

121212131348

104104767676

60,6876

60,61105

62,107,108106106106106106106106106106109

r35O



Enzyme1

,4 wam

AvrQBall

BamHl

BM

BfcvflBceflBell

Bgtl


£coT22INsil

Mscl

AadAaelAcaUAccEBlAinUAUT./U/12257I/UH2258IAipTIIBamFlBamKIBamNIBsplOlflsp46Ifljp98IBsllBstQlCellDdslGdolGinlGoxlGsemMlelNasBlNspSMVPae\n\RhARhA\

AlwSX

AtuClBspXHfi«GIBstKlCpelQhlFbalPoviSselSstTV

Vanl

Sequence

ATGCATATGCAITATGCAtTCtCTAGGTGGICCATGGCCAGIGATCCGGATCCGGATCCGGATCCGIGATCCGGATCCGtGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGIGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGGATCCGtGATCCGGATCCGGATCCGGATCCGCAGC(8/12) 2(5),-2(5)GCAGC(8/12)GAAGAC(2/6)ACGGC(12/13)TIGATCATGATCATIGATCATGATCATGATCATGATCATGATCATGATCATIGATCATGATCATGATCAGCCNNNNtNGGCGCCNNNNNGGC

Me3

site

4{5)

5(4)

Commercial4

source

GUBMNRNABGIN

ABGIMNPRU

GP

GIN

BGIMNPU

BGIMNPRU

Reference

110,111112113

82,11432,115

27116-118

11911968

12068

12112212268

12312390336933

124,1257068

1261191271195425258551

128129

116,130-13213313413513613713813970

140141105

98,14254

143144-148

149

r351



Enzyme1

Bgia

Binl

BsaMflwBIBsePl

Bsm\BsmM

BspHl

flipMlBspim

BsriBstEa

BstXl

CauU


NspMACl

AhviBthU

fijoPIBsrmBsiHU£co1431EspTIEspSlKpn30l

Ubd26l

RspXl

Accmfifc/74111Bfo7920ICauBilKpri2lMro\

AcrilAspAlBsGUBstDlBslPlCfiTiCfr\9lEcalEco9il£coO65IKoxlNspSAHSdM

flisGIflflTI

AhalAseEAspllBcnlBspllBsp%l

Sequence

AIGATCTAIGATCTGGATC(4/5)GGATC(4/5)GGATCYACGTRGATNNNNATCGCGCGCGCGCGCGCGCGCGICGCGCGCGCGCGCGCGCGCGCGCGCGCGCGAATGC(1/-1)GTCTCGTCTC(l/5)TICATGATICATGAACCTGC(4/8)TICCGGATtCCGGATCCGGATCCGGATICCGGATtCCGGATICCGGAACTGG(1/-1)G1GTNACCGIGTNACCGIGTNACCGGTNACCGGTNACCGIGTNACCGGTNACCGGTNACCGIGTNACCGIGTNACCGIGTNACCGGTNACCGIGTNACCGGTNACCCCANNNNNINTGGCCANNNNNNTGGCCANNNNNNTGGCCtSGGCCISGGCCISGGCCSGGCCISGGCCSGGCCSGG

Me3

site

2(4)

Commercial4

source

ABGIMNPRU

N

N

GNU

GNN

NGNNAGR

UNBGMNPU

G

GNRU

A

Reference

144,148,15015115215315471

155139139139

139,1561296969

1576671

158159160

153,161153,16166,162

163163164

33,165166167

168,1696836

17070

1714347

172173,174175,176

1778568

139,178139139

95,96,1798

180129

181-1863333

r352




SequenceMe3

siteCommercial4

sourceReference

Cfri

cynoi

Oa\

CvOl

DdADprd*

DnOl

flsp55IEciDlEcoUllEco\T91£col90IHgiS2UHMlNeilPae\mRshUSsp2lTmull

Qi-141Cfr3SlQT-391CfiAOlEaelEciBlEco901Eco\64lEcom

Bsp21I

AsplVTlBanUlBardBdil

mmBsclBsp2lBsjAlBspMlfijplO6IfispXIOp4I

CviKlCviUCVJMI

CWNICvrOI

Cfid*NanU*NgoDm*NmuDl*NmuEl*NsuDl*

CCSGGCCSGGCCSGGCCSGGCCSGGCCSGGCCSGGCCtSGGCCSGGCCSGGCCSGGCCSGGYIGGCCR

YGGCCRYGGCCRYGGCCRYGGCCRYtGGCCRYGGCCRYGGCCRYGGCCRYGGCCRRICCGGYRCCGGYATtCGATATCGATATCGATATICGATATICGATATCGATATtCGATATCGATATCGATATCGATATtCGATATtCGATATCGATRGICYRGCYRGCYRGCYRGCYRGCYCtTNAGGAITCGAITCGATCGATCGATCGATCGATCRGtGNCCY

BGMNU

4(5)

4(5)

2(5)

5(6)

GMN

AU

ABGMNPR

U

3(5)

1(5) BGIMNPRUABGIMNR

GM

6934

18751

187188189

190,1915193

129173

44,186,192,19343464747

194,19534

17312928

43,44,186,1%173

197,198885

199200201

36,202333369

20313869

204,205206206206206206

207-209210-212

213,21421521657

21757

218-220

r353



Enzyme1

DramDrdlDrdaDialEdlEco3U

EaATta

£co57I

£c»NI

EcoRI


&wO109IPsslVneM

BsalEceAllEco5UEco95lEaffn&O101IEcol20lEcoxrnEco\291Eco 1551Ecol56lEcol5Tl£col62I£col851&»191IEco2031&O204I£co205IEcolXTlEco225lEco233l£co239IEco240\EcoUMEco246l£co247IPpalSau\2l

AM

Bsp6UEcol25lFsfl

finWI

Eco82l£col59IEco2281Eco2371£co252IRstiSsol

Sequence

RGtGNCCYRGGNCtCYRGGNCCYCACNNNIGTGGACNNNNINNGTCGAACCACtCRYGGTCCGCCGGTCTC<l/5)GGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCGGTCTCAGCIGCTAGCtGCTCTGAAG(16/14)CTGAAGCTGAAGCTGAAGCCTNNINNNAGGCCTNNNNNAGGGIAATTCGAATTCGAATTCGAATTCGAATTCGAATTCGIAATTCGIAATTC

Me3

site

3(6)

2(6)

Commercial4

source

AGNUI

MNN

AU

N

ABGIMNPRU

Reference

221126,222

149218-220

22322322434

22571

1871292262271871875151

1871871875151

18718718718748

12933

12948483333

15317353

22822917351

230231

71232-234

2265148

129129

235-237238,239

r354



Enzyme' Isoschizomers Recognition2

SequenceMe3

siteCommercial4

sourceReference

fi»Rn5

AeulAorlApy\AluWAxuSlAtuO.BinSlftp56Iftp 1031ftrGOflrtNIflHOIamiCfrSlCfrUl

CfiwnCfiQ2\Cfi2MCfrVSlCfiQJlCfr2»lCfiQSXcfryoiQr31IQr35IQTS37ICthUEagKlEcaaEctaEcttel£c/136I&/137H£WS39I£co38IEaAOlEaAllEcoGOlEco6UEco67lEcolQlEcolWEco\2%\EcoMOlEcol93lEco2mEco2mEspllFspKMlJfatl65IKpnlOlKpnUl

1 CCWGGCCIWGGCCIWGGCCIWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCIWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCIWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCWGGCCIWGGCCWGGCCWGGCCWGG

2(5) BG

M

240-2422431192441732452461546933

139247

70157

43,4443,44

47474747474747474747

188248249

66250227

511871885252524444

226226251

51129187333333

2521295151

r355



Enzyme1

+

EcoRV

Espl

£sp3IFin\FniAm

Fold

GdtUGsui


KpnUlKpnlfAMphlMvcASgrUTaqXlZanl

Bspl6lfli/RICeqlEcdill£col78IHjalNanlNflMNsiCl

CeiaSari

Bsp6lFbriUui960I

AccUBceFlBceRlBeplBsp50lBsplQlBWUIflsull92nflsull93I&u 15321fis«6633IBsuEnQwAIFspMlHM0561MvaMMvnlPftAlSeelThai

HinGUU

Bco35l

Sequence

CCWGGCCWGGCCWGGCCIWGGCCWGGCCtWGGCCIWGGGATIATCGATATCGATATCGATIATCGATtATCGATATCGATtATCGATATCGATATCGATtATCGCITNAGCGCTNAGCGCTNAGCGAGACGGTCCCGCtNGCGCtNGCGCtNGCGCtNGCCGtCGCGtCGCGCGCGCGCGICGCGCGCGCGCGtCGCGCGCGCGCGtCGCGCGCGCGCGCGCGCGCGCGCGCGCGICGCGCGCGCGCGICGGGATG(9/13)GGATGYGGCCG(-5 / - l )CTGGAG(16/14)CTGGAG

Me3

site

2(4)

2(6)

1(5)

1(5)

3(6)

Commercial4

source

AU

ABGIMNPRU

GM

N

AG

N

BIAGIMN

Reference

187187253

254,255256257258

259-2613370

26244,263

5126421526526626768

26869

153269270105271272

6,273274123

275,27669697065

65,123277

123,27865,279,280

2811532821042832768

284285,286

287-289290

229,29148

r356




SequenceMe3

siteCommercial4

sourceReference

HaelHatW

florin

Bspttl&p28I

Bmel42IHinlUNgol

AcalWAspl42lAspTmBcelMBco331BillBluUBselBMBshMBshBlBshClBshDlBshElBshFlBsplUBspUllBsp226lfispRIfosCIBstClBstJlBsu 10761fisulll4IBsuRlBtelOmlOilCsplXDsaUFwSlFnuDlHhg\MntlMnnUNgoUNgoPUNgoSlNlalPcdlPallPpulSfal

Spun

CTGGAGCTGGAGWGGtCCWRGCGCtYRGCtGCYRGCGCYRGCGCYGGICCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGtCCGGCCGGICCGGCCGGCCGGCCGGCCGGCCGGICCGGCCGGCCGGtCCGGCCGGtCCGGCCGGtCCGGCCGGCCGGCCGGCCGGICCGGCCGGCCGGCCGGtCCGGCCGGICCGGCC

3(5)

ABGIMNP

ABGIMNPRU

3(5)

17333

292293,294

295296297

298-30068

18868

30133302290303103103103103103103103301301301

304-30613913970123123

300,307,30830997

31049

22410527266

253311312266313314127

315,316127317318

r357



Enzyme1

HgalHgiM

HgiCl

HgiEHHgiSU.


SualSul\TspZNlTteMTtnlVhtAVnil

AspWUbdlll

BanlEcoXlEco64I£col68IEcolG9lEcollUEco\13lEco\9SlEspllEsp6lEsp91EsplOI£jplllEsp\21Espl3lEjpl4I£^151Esp22lHgiHl

BanUfljpU7Ifire 18541BvulCfrWlEcolAlEcolSlEco26lEa>351Eco68l

Eco\mEcolSOlEcolUlEco2\5\£co216IEcoinx£coT38IEcoTm£coT93I£i»T95I

Sequence

GGICCGGCCGGCCGCKXGGCCGGCCGGCCGACGC(5/10)GWGCWtCGWGCWICGWGCWICGIGYRCCGtGYRCCGGYRCCGtGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGGYRCCGIGYRCCACCNNNNNNGGTGRGCYICGRGCYICGRGCYCGRGCYtCGRGCYICGRGCYCGRGCYICGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYCGRGCYC

Me3

siteCommercial4

source

NNM

GIMNPU

GIMNPRU

Reference

319320321322102128149

217,296,323324325

3812,3265,326

129226,327

1871295151

1874869696969696969

1291291312135

3327732847

157,32915744

1572271875133

17317348

112330330330

r358




SequenceMe3

siteCommercial4

sourceReference

Hhai.

HindU

HimSE

Hinfl

Hpal

KoxU

BcalCfo\FnuDUlMn6IHinTlHinGUlHinPUHinSUHinSttMn/iIVSriNI

ChuUHinll60EHinU6lUHMClHindiMnnl

Asp52lBbriBpelflnFIcfmiOmlEco6SlEcoWlEco\mEco23HEcoVTHHinSmHin\TS\HmlO76m/finJCDHinbmHinfUHsulMtil

CviBlCviClCviDICviElCviFlCvrGIFnuMHhaUNadNenHNsim

GRGCYtCGCGICGCX5CGCGICGCGICGtCGCGCGCGCGCGICCX:GCGCGCGCGCGCGICGCGTYtRACGTYRACGTYRACGTYRACGTYtRACGTYtRACGTYRACAtAGCTTAAGCTTAAGCTTAAGCTTAtAGCTTAAGCTTAAGCTTAAGCTTAAGCTTAAGCTTAAGCTTAtAGCTTAAGCTTAAGCTTAAGCTTAAGCTTAAGCTTAAGCTTAtAGCTTAAGCTTGtANTCGtANTCGANTCGANTCGANTCGANTCGANTCGtANTCGtANTCGANTCGANTCGANTCGTTIAAC

2(5)

5(6)

1(6)

2(6)

5(6)

ABGNPRU

BIMR

N

M

ABGINPRU

ABGIMNPRU

ABGIMNPRU

ABGIMNPRU

177331,332

28126272333

15289,334

335335335311336

337-340341282282342343311

337,338,34418866

345,34634747

34122722718748

34815

289282342

66,349350

66253

349,351,352353353353353353353272

354-356357357358

359-361

r359




SequenceMe3

siteCommercial4

sourceReference

HpaU

Hphl

Kpnl

Ksp632I

Mael

MaeUMaelllMbot+

BseU

Ajp748IBspSl&p47IBsp4Slfiipll6Iflre 11921fliuFIFmUHapnHMXHin5\MniQMnolMsplSecUSfaGUl

NgoBl

Asp718I£col49I£spl9IKpnKl41NmilSou 101SMStfiAISlhBlSthClSthDlSthElSthFlSthGlSthUlSthJlSlhKlSthUSthMlSthSl

Eari

Mjal

Bct2Ai\fisoPI&p9Iflsp 181fln>491

GTTAACCICGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCCGGCICGGCCGGCCGGCCGGCICGGCICGGCCGGCCGGGGTGA(8/7)GGTGAGGTAC1CGIGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGIGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCGGTACCCTCTTC(l/4)CTCTTC(l/4)CITAGCTAGAICGTIGTNACIGATCIGATCGATCGATCGATCGATC

2(5)

1(5)

1(5)

-2(5)-2(5)

BGMNPRU

AGI

ABGIMNPRU

N

ABGIMNPRUM

MNM

MMBGINP

303298,359,360

18833696933

65,12365,123,279

153296,362

18915

25366,363

132,364,365366367

349,368,369371,372373,374

3755148

188376173

377,378106106106106106106106106106106106106

330,37737938038155

381381382383139333369

r36O



Enzyme' Isoschizomcrs Recognition2

SequenceMe3

siteCommercial4

sourceReference

fisp51IBsp52lfisp54IBsp571fisp58IBsp591&p60IBsp6UHsp64IBsp65lBsp661Bsp6Tlfljp72Iflsp74Iflsp76IBsp9UBsp\05lBspmifispAIBsrPBBssGUBstESEBstXIlCpai.CpflCspSlCxylCviAICviBDCviHIDpnUFnuAHFnuClFnuElHac\MailMkrMMmeUMnomMoilMspGIUMspBlMMMthMNdeQNflWNflBlNfllNlaDlNlaBNmeClNphlAWAI

GATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCIGATCGATCGATCGATCGATCIGATCGATCIGATCGATCGATCGATCGATCGATCIGATCGATCGATCtGATCGATCGATCGATCGATCtGATCtGATCtGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCIGATCGATCGATCGATCGATCIGATCIGATCIGATCGATC

2(6)

BG

6969696969696969

3016969

30169

30130169

3016942

139139

66,169,384139306220

69281

385,386387206

210,21266,272

11127238825

38939066

38241

10413910435726539135756

314266266392

r361



Enzyme1

+

++++++

MboQ

MfelMM

AflylMme\AMIMselMstI

MwolNoel

Isoschizomcrs Recognition2

NspAlNsulPfiilRlullSalMSalHISauSMSau6imSauCISauDlSauElSauFlSauGlSauMlSinMITruU

NculTcA

ApelUba6\

AcaUiAoslAviaOca

amFdiaFsplGspAENspKUlNspUAfjpMI

AmeEAniMlApeAlApriEcoSdlEsp5lAfcslNasWlNbalNbriNgoMlNmiFl

Sequence

GATCGATCGATCGATCGATCGATCIGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGAAGA(8/7)GAAGAGAAGACAATTGAtCGCGTACGCGTACGCGTGASTCTCCRAC(2O/18)CCTC(7/7)TITAATGCIGCATGCGCATGCtGCATGCIGCATGCGCATGCGCATGCtGCATGCIGCATGCGCATGCGCATGCGCATGCGCAGCNNNNNINNGCGCCIGGCGCCGGCGCCGGCGCCGGCGCCGGCGICCGGCGCCGGCGCCGGCGCCGGCGCCGGCGCCGGCGCCGGCGCCGGC

Me3

site

4(5)

5(6)

Commercial4

source

ABGIMNPRU

BGINP

ABGIMNPRU

GNN

GM

GN

GMNU

Reference

2557

365173231231

393,39439539639639639639639757

109382,398-400

4013204022852314837

390132,403

404130,405

689

626861

101,10274,406

10362

60,6160,68

407408

271532725

44,409173169256565

41057

r362



Enzyme1

Nari

Ncol

NdelNhelMom

NlaW

NodNrul

Nspl

NspBUPflmPlelPmaCl

PpuM

Pstl


NmulNspWNlaSUPgRPsp6URlu\SacMSalClSaolSauMSauBMKISkal

BbeMBbelBinSUEcomEhelNamlNdalNunUSfol

NspSAm

HinlUHMO.

BcriBsp29lBsslRluil

AmalBsp6SlSalDlSbol3\

NspW.

BspSTlEcoTllPmR

Uball

AM

Sequence

GCCGGCGCCGGCGCCGC3CGCCGCX:GCCGGCGCCGGCGCCGGCGCCGGCGCCGGCGCCGGCGCCtGGCGCCGGCGGICGCCGGCGCCGGCGCtCGGCGCCGGCtGCCGGCtGCCGGCGCCGGICGCCGGICGCCGGCGCCCICATGGCCATGGCAITATGGICTAGCCATGICATGICATGGGNtNCCGGNNCCGGNNCCGGNNCCGGNNCCGCIGGCCGCTCGICGATCGCGATCGCGATCGCGATCGtCGARCATGIYRCATGIYCMGICKGCCANNNNINTGGGAGTC(4/5)CACIGTGCACGTGCACIGTGCACGTGRGIGWCCYRGIGWCCYCTGCAIGCTGCAG

Me3

site

5(6)

Commercial4

source

BGNP

A

G

ABGIMNPR

BGNPBGMNPRN

N

AGIMNPRUABGIMNPU

AGGNN

NN

ABGIMNPRU

Reference

35725

10541141

412-4145027

415391416

57417154418154419

420,42142242313915313985

424376314425

15314228337148

426,4273761286927

112597676

153428429

69430431

153,43218

374,401,43368

r363




SequenceMe3

siteCommercial4

sourceReference

AU2S821/U/AJIAsp36lAjp708IAspTlBbilBee 1701fljpl7Iflsp43Ifisp63Ifijp78Iflsp81IRsp93IfisplO7I&plO8IBspBlBsuBlCaulRCfllCfrAAlCIA

onEaePlEarn£W133I£c/593I£co48IEaA9lEcoSil£col61I£col67IEspSUEsp\A\\GseQKpn\2\MaulMMNas\NgblNodPma\PmaUlPmyiSalPlsjnStallXmaUXoiiXphlYenlYenAlYenBlYenCl

CTGCAGCTGCAIGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAtGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAIGCTGCAGCTGCAGCTGCAIGCTGCAIGCTGCAGCTGCAIGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAtGCTGCAtGCTGCAGCTGCAGCTGCAGCTGCAGCTGCAIGCTGCAGCTGCAGCTGCAG

122434157886811

1233348

3013016969333342

123,435436388437

68438274227

51188129129227

5151

1731875451

12725

10525

376132439440

441,442253

57443444445446446446446

r364




SequenceMe3

siteCommercial4

sourceReference

Pvul

PvuB

Rsal

RsrH

Sacl

SacU

YenD\YenEl

BmalBmaMBmaBlBmaClBmaDlQr51IDrdmEcUlNbaRMRsplXmRXmlMXiulXora

BavlCfrtlGsplMaiNmeHlSbalSdAII

Csp6lCviQI

CpolCspl

£c/136D£yi37I&0ICRINasSiScolSsA

AosmBadBsp\2l

cfrynCftAUCft42lQ/43ICfrtsaCsclPrdMDrdBlDrdClDrdEl

CTGCAGCTGCAGCGATICGCGATCGCGATCGCGATCGCGATCGCGATCGCGATCGCGATCGCGATCGCGATtCGCGATICGCGATCGCGATCGCGATCGCGATCGCGATtCGCAGtCTGCAGtCTGCAGICTGCAGCTGCAGCTGCAGCTGCAGCTGCAGCTGGTtACGTACGtTACCGIGWCCGCGGWCCGCGGWCCGGAGCTICGAGICTCGAGCTCGAGCTCGAGCTCGAGCTCGAGCTICCCGCIGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCtGGCCGCGGCCGCGGCCGCIGGCCGCGGCCGCGGCCGCGGCCGCGG

ABGMNPRU

4(4)

4(4)

BABGIMNPRU

ABGIMNPRU

BGN

AGIMNPRU

BINPRU

446446447

27,448,4492827272733

223450132451452453453311

444,447447,454

45543,44,454

68456457

330,37768

458129459460

27461462

18187127105415

463,464462

6266,310

334646144747

465223223223466

r365




SequenceMe3

siteCommercial4

sourceReference

Sail

Said

DrdFlDrdGlDspHEcclEcasiEcDTlEco55l£co92IEco96lEco99lEcolOOlEcolOMEco\M\&»135I£col58I£c»182IEcol961£co208IGallGceGUGcelKsp\MralNgomNgoDlNgoPUlNlaDUlNlaSlPaeAlSaalSab\SaidSbolSfiiShylSseUSstUTgH

HgiCmHgiDUKoylNoplRhelRhplRHdRrolXamlXdl

AodAxylBsu36l

CCGCGGCCGCGGCXGCGGCXX3CGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCtGGCCGCtGGCCGCIGGCCGCIGGCCGCGGCCGCGGCCGCGGCCGCIGGCCGCGGCCGCGGCCGCIGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCGGCCGCIGGCCGCGGGITCGACGITCGACGITCGACGTCGACGITCGACGTCGACGTCGACGTCGACGTCGACGTCGACGITCGACCCITNAGGCCITNAGGCCITNAGGCCITNAGG

ABGIMNPRU

M

GN

223467

4866,468

15752

1292272272271872515151

187515133

388469388470471472216266

5616

437,473153415

50474,475474,475

47654

463131477

1212

4781321281286565

477479480

62481482

r366



Enzyme1

Seal

ScrFl

Sdul

Serf

SJaMSfelSfiiSmal

Snahl

Snal

SpelSphl

spa

Isoschizomers

CvnlEdClEcol6lEcoSU£coU5IEcoll8IMstnOxaNlSeem

/4jp763IfinMI

flsp53Ifisp73I£i»43IEcoSmEcoSO\EcoiSlEco93l£col53IEcolOOlMsp6TlSsoU

AocUflipl2861NspU

Bsail

Ahy\Cfi9lCfriAl£WRIXcylXmal

EdMEcolOSlEcol5m

Xcal

BbulBsp\2UPae\SpaXl

Recognition2

Sequence

CCITNAGGCCTNAGGCCTNAGGCCITNAGGCCTNAGGCCTNAGGCCITNAGGCCITNAGGCCTNAGGAGTIACTAGTACTAGTACTCCtNGGCCNGGCCNGGCCNGGCCNGGCCNGGCCNGGCCNGGCCNGGCCNGGCCINGGtCCNGGGDGCHICGDGCHICGDGCHtCGDGCHtCCtCNNGGCCNNGGGCATC(5/9)CITRYAGGGCCNNNNINGGCCCCCIGGGCICCGGGCICCGGGCCCIGGGCCCGGGCICCGGGCICCGGGTACIGTATACGTATACIGTATACGTAGTATACGTAITACAICTAGTGCATGICGCATGICGCATGCGCATG1CGCATGCCIGTACG

Me3

site

2(5)

3(5)

2(4)

Commercial4

source

B

AU

ABGIMNPRU

GMN

GNUP

N

G1NPRUABGIMNPRU

INRGMN

BMNRABGIMNPRU

A

Reference

483,48434

227485187187132133366

486,48718870

4886969

4891292274892271875141

238,490491,492

6266,123,493

59366494137495496

443,497,498431

43,44,497499500501443502

3418

187503504376505506

33507127318

r367



Enzyme1

SsplSail

Styl

Taql

TaqW

7ip45ITspEl71/illH

7WillinVspl

XbalXcmlXhol

koschizomers

Pfiil

AatlAspUlOiylEcoWlGdilNlaSl

£»130I£x>2O8II£coT14I£coT104ISblMSblBlffl/CI

CViBfflijn77AHB8I

AsplFsulNtalSpinTsplT)elTtti

AselAsnl

AbriAsjAllAspKBlBadlBbflBbimBlulBsjffllfluHIflnHIBstUBstVlBsuMl

Recognition2

Seq^nce

CXJTACGAATtATTAGGICCTAGGICCTAGGCCTAGGCCTAGGICCTAGGtCCTAGGCCTCICWWGGCICWWGGCCWWGGCICWWGGCCWWGGCCWWGGCCWWGGCCWWGGTICGATCGATCGATICGAGACCGA(ll/9)CACCCA(ll/9)GTSACAATTGACNINNGTCGACNINNGTCGACNNNGTCGACNNNGTCGACNNNGTCGACNNNGTCGACNNNGTCGACNNNGTCCAARCA(ll/9)ATITAATATITAATATITAATTICTAGACCANNNNNNNNNTGGCITCGAGCITCGAGCTCGAGCTCGAGCTCGAGCTCGAGCTCGAGCITCGAGCTCGAGCTCGAGCTCGAGCTCGAGCTCGAGCTCGAG

Me3

site

4(6)4(6)

4(6)

5(6)3(5)

Commercial4

source

BMNRABGIMNPRU

BGMNR

A

BGIMNPRU

A

AGINP

N

ABGIMNPRU27ABGIMNPRU

Reference

27487508

518828

33,509290105510

33,51133

512512330330330

513,514387513

513,515-51766,518

51952052147041510531871

521521522523180524525

52652718888

16316311

52669

13913970

528,529123,279

r368



Enzyme1 Isoschizomers

BthlCertDdeYS.DrtlDlMealMedMpulMsilPaeRTlPanlpjmPflm&U3239ISalSailSerfSgalSgolSlalSlulSpdlSsp4lXpa\

XhoUAUMAimBstYlMfll

XmaUiAnalAaa\fijrZIEaglEcVaEco52l

XmnlAsplOOl

Recognition2

Sequence

CTCGAGCITCGAGCTCGAGCTCGAGCTCGAGCTCGAGCTCGAGCTCGAGCITCGAGCITCGAGCTCGAGCTCGAGCITCGAGCTCIGAGCTCGAGCTCGAGCTCGAGCTCGAGCITCGAGCTCGAGCTCGAGCTCGAGCITCGAGRIGATCYRGATCYRGATCYRIGATCYRIGATCYCIGGCCGCIGGCCGCIGGCCGCGGCCGCIGGCCGCIGGCCGCIGGCCGGAANNINNTTCGAANNINNTTC

Me3

site

5(6)

4(5)

Commercial4

source

N

GM

NAB

N

AUGNM

Reference

15453066

46688

456456

66,349531,532

12728

533534,535

41474474474474536475474

48526

131,282,53727

228538539

32,54054154170

542543

44,10065,544

88

Type i n enzymes

Enzyme Isoschizomers RecognitionSequence

Me3

siteReference

EcoPlSlEcoPl

Hinel

CAGCAGAGACCCGAATCGAAT

3(6)565,566

567-571572,573

574

r369



FOOTNOTES

1. • signifies that Dpni and its isoschizomers require the presence of 6-methyladenosine within the recognitionsequence GATC.2. Recognition sequences are given using the standard abbreviations (575) to represent ambiguity:

R = G or AY = C or TM = A or CK = G or TS •= G or CW = A o r TH = A or C or TB = G or T or CV = G or C or AD = G or A or TN = A o r C o r G o r T

3. The she of methylation by the cognate methylase when known is indicated as follows. The first number showsthe base within the recognition sequence that is modified. A negative number indicates the complementary strand,numbered from the 5' base of that strand. The number in parentheses indicates the specific methylation involved.(6) = N6-methyladenosine (5) = 5-methylcytosine (4) = N4-methylcytosine.4. Commercial sources of restriction enzymes are abbreviated as follows:

A Amersham (8/88)B Bethesda Research Laboratories (1/89)G Anglian Biotechnology Ltd. (9/88)I International Biotechnologies Inc 9/88)M Boehringer-Mannheim (1/89)N New England Biolabs (2/89)P Pharmacia P-L Biochemkals (2/89)R Promega Biotec (10/88)U United States Biochemical Corporation (2/89)

5. EcoRB isoschizomers fall into two classes based upon their sensitivity to methylation. EcoRll will not cleavewhen the second cytosine in the recognition sequence is methylated to 5-methylcytosine whereas Mval will cleavesuch a sequence. Isoschizomers of EcoRII that are like Mval are indicated by +.6. Mbol isoschizomers fall into two classes based upon their sensitivity to methylation. Mbol will not cleavewhen the recognition sequence contains 6-methyladenosine whereas SOH3AI will not cleave when its recognitionsequence contains 5-methylcytosine. Isoschizomers of Mbol that are like Sau3AI are indicated by +.7. TaqU differs from other restriction enzymes in recognizing two distinct sequences: GACCGA(11/9) andCACCCA(ll/9).

Alphabetical listing of Type II restriction enzymes

Aaal (XmaDI) AacI (BamHI) Aael (BamHI) AamIAatI (StuI) Aatll AbrI (Xhol) Acal (AsuII)AcaU (BamHI) Acam (MstI) AcalV (HaelH) AccEBI (BamHI)AccI AccH (FnuDD) Accm (BspMH) Acrl (Aval)Acrll (BstEII) Acyl AcyH Aeul (EcoRII)Afll (Avail) AflU AfUU Ahal (CauH)AhaD (Acyl) Ahaffl Ahyl (Smal) Aim!AinI (PstI) AinD (BamHI) AitAI (XhoU) AitI (Eco47m)AMI (XhoII) Alil22571 (BamHI) Alil2258I (BamHI) Ali2882I (PstI)AliAJI (PstI) Alii (BamHI) Alul Alwl (Bin!)AhvNI AlwXI (Bbvl) Amal (Nrul) Amel (ApaLI)Amen (Nael) Anil AniMI (Nael) AocI (Saul)AocD (Sdul) Aorl (EcoRII) AosI (MstI) AosII (Acyl)Aosin (SacII) Apal ApaU ApeAI (Nael)Apel (MhJ) AprI (Nael) Apul (Asul) Apyl (EcoRII)

r37O



AquI (Aval)AsplI (CauII)Asp697I (Avail)Asp708I (PstI)Asp763I (Seal)AspBH (AvaQAspDH (Avail)AspTTI (BamHI)AsuDAtuBI (EcoRII)AtuD (EcoRII)Avil (AsuII)Axyl (Saul)BaJIBamNl (BamHI)BanlLI (Clal)Bbel (Narl)Bbfl (Xhol)BbiTVBcal (Hhal)Bce243I (Mbol)BeenBco33I (HacHI)BepI (FnuDII)BinSI (EcoRII)BlolBmaBI (Pvul)Bmel42I (Haell)BmelBsaAIBsaPI (Mbol)BsePIBshDI (Haem)BsmAIBsplO3I (EcoRII)BsplOSI (PstI)Bspl20I (Apal)Bspl2I (SacII)Bspl8I (Mbol)Bsp22I (Gsul)Bsp3OI (BamHI)Bsp48I (HpaO)Bsp51I (Mbol)Bsp55I (CauII)Bsp59I (Mbol)Bsp63I (PstI)Bsp67I (Mbol)Bsp7OI (FnuDII)Bsp74I (Mbol)Bsp81I (PstI)Bsp8I (CauII)Bsp98I (BamHI)BspBD (Asul)BspRI (Haem)BsrIBssGI (BstXI)BssI (NlaTV)BstBI (AsuII)

Asel (Vspl)Asp36I (PstI)Asp700I (XmnI)Asp718I (Kpnl)Asp78I (StuI)AspCI (Aval)AspHI (HgiAI)AspTm (Haem)Asum (Acyl)AtuBVIAvalAvill (MstI)Bac36I (Asul)BamFI (BamHI)BamNxI (Avail)Bavl (PvuII)BbellBbil (PstI)BbrI (HindDI)Bee12291Bce71I (Haem)BcUBco35I (Gsul)BgllBinSII (Narl)Blul (Xhol)BmaCI (Pvul)Bme205IBpcl (Hindm)BsaBIBscI (Clal)BshAI (Haem)BshEI (Haem)BsmIBsplO5I (Mbol)Bspll6I (Hpall)Bspl21I (SphI)Bspl2IIBsp211I (HaeOI)Bsp28I (Gsul)Bsp43I (PstI)Bsp49I (Mbol)Bsp52I (Mbol)Bsp56I (EcoRII)Bsp5I (Hpall)Bsp64I (Mbol)Bsp68I (Nrul)Bsp71I (Haelll)Bsp76I (Mbol)Bsp82I (AsuII)Bsp91I (Mbol)Bsp9I (Mbol)BspHIBspXI (Clal)BsrPIBssGD (Mbol)BssPIBstCl (Haem)

AseU (CauII)Asp47I (Xhol)Asp703I (Xhol)Asp742I (Haem)AspAI (BstEII)AspCU (Avail)Aspl (Tthllll)AstWI (Acyl)Atull (EcoRII)AtuCI (Bell)AvailAvrl (Aval)BacI (SacII)BamHIBanI (HgiCI)BbeAI (Narl)BbeSIBbiD (Acyl)BbvlBcel4579IBceR (FnuDII)Bcml (Clal)Bcrl (NlalV)BglllBla7920I (BspMII)BluII (Haelll)BmaDI (Pvul)Bme2l6I (AvaU)BprIBsal (Eco31I)Bsel (Haem)BshBI (Haem)BshFI (Haelll)BsoPI (BsePI)BsplO6I (Clal)Bspll7I (Hgilll)Bspl22I (Mbol)Bspl6I (EcoRV)Bsp21I (CfrlOI)Bsp29I (NlalV)Bsp46I (BamHI)Bsp4I (Clal)Bsp53I (ScrFI)Bsp57I (Mbol)Bsp60I (Mbol)Bsp65I (Mbol)Bsp6I (Fnu4HI)Bsp72I (Mbol)Bsp78I (PstI)Bsp84I (Clal)Bsp92I (Xhol)BspAI (Mbol)BspMI

BspXn (Bell)BsrPH (Mbol)BssHI (Xhol)BsOlI (BstEII)BstDI (BstEII)

Asnl (Vspl)Asp52I (HindlU)Asp707I (Clal)Asp748I (Hpah)AspBI (Aval)AspDI (Aval)AspTI (PstI)AsulAtuAIAtuIAMIAvalllAvrllBadI (Xhol)BamKl (BamHI)Banfl (HgJII)BbeAnBbf7411I (BspMII)Bbim (Xhol)BbvIIBee 1701 (PstI)BceRI (FnuDII)Bcnl (CauII)Bdil (Clal)Bin!Blil (Haelll)BmaAI (Pvul)Bmal (Pvul)Bme899IBpulBsaJI (Seel)Bsell (Hpal)BshCI (Haelll)BshI (Haelll)BsplOOI (Avail)BsplO7I (PstI)Bspl 191 (AsuII)Bspl286I (Sdul)Bspl7I (PstI)Bsp226I (HacDI)Bsp2I (Clal)Bsp47I (Hpall)Bsp50I (FnuDII)Bsp54I (Mbol)Bsp58I (Mbol)Bsp61I (Mbol)Bsp66I (Mbol)Bsp6U (Eco57DBsp73I (ScrFI)Bsp71 (CauII)Bsp87I (PmaCI)Bsp93I (PstI)BspBI (PstI)BspMIIBsrHI (BsePI)BssCI (HaeUI)BssHn (BsePI)BstAIBstH

r371



BstEUBstGH (EcoRH)BstKI (Bell)BstOI (EcoRH)BstSI (Aval)BstWI (EcoNI)BstZI (Xmam)Bsu 11921 (Hpell)Bsu 15321 (FnuDII)BsuBI (PsU)BsuRI (Haem)Bvul (HgUII)CauIICeU (BamHI)Cfol (Hhal)Cfrl4I (CM)Cfr23I (Asul)Cfr28I (EcoRII)Cfr32I (Hindffl)Cfr38I (Cfrl)Cfr42I (SacII)Cfr46I (Asul)Cfi-511 (Pvul)Cfr6I (PvuII)CfrA4I (PstI)Cful (Dpnl)Chyl (StuI)Oil (Avail)ClmD (Avail)Cpal (Mbol)CscI (SacII)Csp5I (Mbol)CsulCvelCviDI (Hinfl)CviHI (Mbol)CviLI (CvUI)CviPIDdelDpnlDrainDrdDI (Xhol)DrdIDsall (Haem)Eagl (XmalE)Ecal (BstEII)EciBI (Cfrl)EcUEel 1371 (SacI)Ecl593I(PstI)Ecin (EcoRII)EcolOOI (SacII)Ecoll3I (HgUII)Ecol21I (CauII)Ecol29I (Eco310Ecol43I (BsePI)Ecol55I (Eco31I)EcolS8D (SnaBI)

BstEm (Mbol)BstHI (Xhol)BstU (Xhol)BstPI (BstEII)BstTI (BstXI)BslXIBsu 10761 (Haelll)Bsull92H (FnuDII)Bsu 18541 (HgUII)BsuED (FnuDII)BthI (Xhol)CallCaum (PstI)Cein (Espl)CfrlOICfrl9I (BstEII)Cfr24I (EcoRII)Cfr29I (EcoRII)Cfr33I (Asul)Cfr39I (Cfrl)Cfr43I (SacII)Cfr47I (Asul)Cfr52I (Asul)Cfr7I (BstEII)CfrlCMClalCliD (MstI)CW (Haem)Cpel (Bell)Csp2I (Haem)Csp6I (Rsal)CthI (Bell)CviAI (Mbol)CviEI (Hinfl)CvUCviMI (CvUI)CviQI (Rsal)Ddell (Xhol)DpnII (Mbol)DrdAI (SacD)DrdH (SacII)DrdDDsplI (SacII)EagKI (EcoRII)EcaD (EcoRII)EciCI (Saul)Eel 1331 (PstI)Eel 137H (EcoRII)Ecl66I (EcoRD)EcUI (Pvul)Ecol01I(Eco31I)Ecol 151 (Saul)Ecol25I (Eco57I)Ecol30I (Styl)Ecol47I (StuI)Ecol56I (Eco31I)Ecol59I (EcoRI)

BstFI (Hindlll)BstI (BamHI)BstMI (Seal)BstQI (BamHI)BstUI (FnuDII)BstXIl (Mbol)Bsulll4I (Haem)Bsu 11931 (FnuDII)Bsu36I (Saul)BsuFI (Hpall)BthH (BinI)CauB3I (BspMII)Ccrl (Xhol)Ceql (EcoRV)Cfrl II (EcoRII)Cfr20I (EcoRII)Cfr25I (EcoRII)Cfr30I (EcoRII)Cfr35I (EcoRII)Cfr40I (Cfrl)Cfr45I (Asul)Cfr48I (HgUII)Cfr54I (Asul)CfrSI (Asul)CfrNI (Asul)Chul (Hindm)Clcl (PstI)

aimClulCpfl (Mbol)Csp45I (AsuII)Cspl (RsrII)Cthll (EcoRII)CviBI (Hinfl)CviFI (Hinfl)CvUICviNI (CvUI)CviQnDdsl (BamHI)Dral (AhallT)DrdBI (SacII)DrdFI (SacII)Drdm (Pvul)Eael (Cfrl)EagMI (Avail)EccI (SacII)EciDI (CauII)Eel 1361 (EcoRII)Ecl28I (SacII)Ecl77I (PstI)EclS39I (EcoRII)Ecol04I (SacII)Ecol 181 (Saul)Ecol27I (Eco3H)Ecol34I (SacII)Ecol49I (Kpnl)Ecol57I (Eco31I)Ecol61I (PsU)

BstGI (Bell)BsUI (Haelll)BstNI (EcoRII)BstRI (EcoRV)BstVI (Xhol)BstYI (XhoII)Bsu 11451Bsu12591Bsu6633I (FnuDII)BsuMI (Xhol)BtU (Avail)Caul (Avail)Cdi27I (EcoRII)Cfll (PstI)Cfrl 31 (Asul)Cfr22I (EcoRII)Cfr27I (EcoRII)Cfr31I (EcoRII)Cfr37I (SacII)Cfr41I (SacII)Cfr45II (SacII)Cfr4I (Asul)Cfr5I (EcoRII)Cfr9I (Smal)CfrS37I (EcoRII)ChuII (Hindll)ClcII (MstI)Clml (Haelll)CpaAI (FnuDII)Cpol (RsrII)Csp4I (Clal)CstI (PstI)Ctyl (Mbol)CviCI (Hinfl)CviGI (Hinfl)CviKI (CvUI)CviOI (CvUI)Cvnl (Saul)DmolDrall

DrdCI (SacII)DrtlGI (SacII)DsalEaePI (PstI)Earl (Ksp632I)EciAI (SnaBI)EciEI (Apal)Ecll36II (SacI)Ecl37I (SacII)EcUEclXI (Xmam)EcolOSI (SnaBI)Ecol20I (Eco31I)Ecol28I (EcoRII)Ecol35I (SacD)Ecol53I (ScrFI)Ecol58I (SacII)Ecol62I (Eco31I)

r372



Ecol64I (Cfrl)Ecol70I (EcoRII)Ecol79I (CauII)Ecol88I (Hindm)Ecol95I (HgiCI)Eco201I (Asul)Eco206I (EcoRII)Eco211I (HgOII)Eco225I (Eco31I)Eco233I (Eco31I)Eco241I (Eco31I)Eco252I (EcoRI)Eco32I (EcoRV)Eco40I (EcoRII)Eco47I (Avail)Eco49I (PstI)Eco52I (Xmam)EcooOl (EcoRII)Eco67I (EcoRII)Eco72I (PmaCI)Eco8II (Saul)Eco88I (Aval)Eco93I (ScrFI)Eco98I (Hindni)EcoICRI (SacI)EcoO44IEcoRV

EcoT38I (HgUII)EcoVm (Hindm)Espl II (HgiCI)Espl4I (HgiCI)Esp22I (HgiCI)Esp5I (Nael)Esp8I (BsePI)Fbal (Bell)RnlFnu4HIFnuDI (HaelO)FoldFspl (MstI)Fsul (Tthllll)GdU (StuI)GinI (BamHI)Gsem (BamHI)Gspl (PvuII)HaeHHapH (Hpall)HgiBI (Avail)HgiDI (Acyl)HgiFIHgiHID (Avail)HgiS21I (CauII)HhllHinll60n (Hindll)Hinin (NlaDI)Hin5D (Asul)Hin8I (Acyl)HinHl (Haell)

Ecol67I (PstI)Ecol71I (HgiCI)Ecol80I (HgUII)Ecol90I (CauII)Ecol96I (SacII)Eco203I (Eco31I)Eco207I (EcoRII)Eco215I (HgUH)Eco228I (EcoRI)Eco237I (EcoRI)Eco246I (Eco31I)Eco25I (HgLFII)Eco35I (HgUII)Eco411 (EcoRII)Eco47H (Asul)Eco50I (HgiCI)Eco55I (SacII)Ecooll (EcoRII)Eco68I (HgUII)Eco76I (Saul)Eco82I (EcoRI)Eco90I (Cfrl)Eco95I (Eco31I)Eco99I (SacII)EcoNI

EcoO65I (BstEII)EcoT104I (Styl)EcoT88I (HgOII)Ehel (Narl)Espl2I (HgiCI)Espl 51 (HgiCI)Esp2I (EcoRII)Esp5D (PstI)Esp9I (HgiCI)FbrI (Fnu4HI)FinD (Hpall)FnuAI (Hinfl)FnuDIIFsalFspU (AsuII)GaU (SacII)OdinGoxl (BamHI)GspAI (A vaH)GsulHaelllHculHgiCIHgiDU (Sail)HgiGI (Acyl)HgUI (Avail)HhalHin 10561 (FnuDII)Hinll6in (HindH)Hin2I (Hpall)Hin5m (Hindm)Hin8D (NlalD)HinJCI (Hindll)

Ecol68I (HgiCI)Ecol73I (HgiCI)Ecol82I (SacII)Ecol91I (Eco31I)Ecol96U (Asul)Eco204I (Eco31I)Eco208I (SacII)Eco216I (HgUII)Eco231I (Hindm)Eco239I (Eco31I)Eco247I (Eco31I)Eco26I (HgUH)Eco38I (EcoRII)Eco42I (Eco31I)Eco47mEco51I(Eco31I)Eco56I (Nael)Eco64I (HgiCI)Eco70I (EcoRII)Eco78I (Narl)Eco83I (PstI)Eco91I (BstEII)Eco96I (SacII)EcoCKIEcoO109I (Drall)EcoRIEcoT14I (Styl)EcoT93I (HgUII)ErpI (Avail)Espl3I (HgiCI)Espl9I (Kpnl)Esp3IEsp6I (HgiCI)EsplFdil (Avail)FinSI (Haelll)FnuAH (Mbol)FnuDffl (Hhal)Fsfl (Eco57I)FspMI (FnuDII)GceGU(SacII)Gdol (BamHI)Gsel (Asul)GspAII (MstI)HacI (Mbol)HagIHgalHgiCU (Avail)HgiEI (Avail)HgiHI (HgiCI)HgUIIHhan (Hinfl)Hinl056nHinl73I (HindDI)Hin3I (CauII)Hin6I (Hhal)HinGUI (Hhal)HinJCD (Hindm)

Ecol69I (HgiCI)Ecol78I (EcoRV)Ecol85I (Eco31I)Ecol93I (EcoRII)Eco200I (ScrFI)Eco205I (Eco31I)Eco208D (Styl)Eco217I (Eco31I)Eco232I (HgUII)Eco240I (Eco31I)Eco24I (HgUII)Eco31IEco39I (Asul)Eco43I (ScrFI)Eco48I (PstI)EcoSin (ScrFI)Eco57IEco65I (Hindm)Eco71I (EcoRII)Eco80I (ScrFI)Eco85I (ScrFI)Eco92I (SacII)Eco97I (Eco310EcoHI (Cfrl)EcoO34IEcoRIIEcoT22I (Avain)EcoT95I (HgUII)EsplOI (HgiCI)Espl41I (PstI)Espl I (HgiCI)Esp4I (Aflll)Esp7I (BsePI)EspHFdiH (MstI)Fnu48IFnuCI (Mbol)FnuEI (Mbol)Fspl604I (EcoRII)FspMSl (Avail)Gcel (SacII)GgllGsell (PstI)GspAmHadHaplHgiAIHgiCm (Sail)HgiEHHgiHD (Acyl)HgiKIHhgl (HacIII)HinlO76m (Hindni)Hinll (Acyl)Hin5I (Hpall)Hin7I (Hhal)HinGUU (Fokl)HinPlI(HhaD

r373



HinSlI (Hhal)HindUHjal (EcoRV)HsalKoxl (BstEII)Kpnl2I (Pstl)Kpn2I (BspMII)Ksp632ILspl (AsuII)Maul (Pstl)Meal (Xhol)MfU (XhoII)MisI (Nael)MkrI (Pstl)MlulMnil (Haem)MnnD (Haem)MnoO (Mnnm)Mpul (Xhol)MselMsp67D (Mbol)MstIMvaAI (FnuDII)Mvnl (FnuDII)Naml (Narl)NasBI (BamHI)Nbal (Nael)Neil (CauH)NdelNflBI (Mbol)NgbI(PstI)NgoDm (Dpnl)NgoMI (Nael)NhelNlal (HaelU)NlaSI (SacII)NmeCI (Mbol)NmelVNmuDI (Dpnl)Nmul (Nael)NopDNphI (Mbol)NsiHJ (Hinfl)NspBDNspEONspHU (Avail)Nspffl (Aval)NspU (MstI)NspMACI (Bglll)NspSAffi (Ncol)NsuDI (Dpnl)NtaSH (NaeOOtuNI (Alul)Pael77I (BamHI)PaeR7I(XhoI)PftI (Mbol)PflNI (Xhol)Pgin

HinS2I (Hhal)HindmHpalHsp2I (Avail)KoxII (HgUII)Kpnl3I (EcoRII)Kpn30I (BsePI)Kspl (SacII)MaelMbolMecl (Xhol)Mfol (Avail)Mjal (Mael)Mlal (AsuII)MlylMnill (Hpall)MnnmMnom (Mbol)Mral (SacII)Msil (Xhol)MspAI (Avail)MstD (Saul)Mval (EcoRII)MwolNanI (EcoRV)NasI (Pstl)NbU (Pvul)NcolNdeD (Mbol)NfU (Mbol)NgoBI (HphI)Ngol (Haell)NgoPO (Haem)NlaDI (Mbol)NlaD (Mbol)NlaSD (Acyl)NmelNmil (Kpnl)NmuEI (Dpnl)NmuSI (Asul)NotINrulNsil (Avaffl)NspDI (Aval)NspFI (AsuII)NspHffl (MstI)NspIV (Asul)NspLH (Asul)NspMI (MstI)NspSAIV (BamHI)Nsul (Mbol)NunIOxal (Alul)Pael81I (CauII)Pail (Haem)POAI (FnuDII)PflWI (Xhol)Plel

HinbHI (HindHI)HinnHpaDHsul (Hindlll)KoyI (Sail)Kpnl4I (EcoRII)KpnlLpnlMaellMboIIMeul (Mbol)MgllMjaH (Asul)Mlel (BamHI)MmelMnllMnnTV (Hhal)MosI (Mbol)Mrol (BspMII)MsUIMspBI (Mbol)MthAI (Mbol)MvilMzil (PvuII)NanU (Dpnl)NasSI (SacI)NbrI (Nael)Ncul (MboII)NflAl (EcoRV)NflHNgoDI (SacII)NgoII (Haelll)NgoPm (SacII)NlaDn (Asul)NlafflNlil (Aval)NmeHNmuAI (Aval)NmuEU (Asul)Noel (Pstl)NovlNsiAI (Mbol)NspAl (Mbol)NspDD (Avail)NspGI (Avail)NsplNspJI (AsuH)NspLIIINspSAI (Aval)NspV (AsuII)Ntal (Tthllll)NunH (Narl)OxallPaeAI (SacII)Pall (Haem)PfUPful (SplI)Pma44I (Pstl)

HincH (HindmHinfll (Hindlll)HphIKoxl65I (EcoRII)KpnlOI (EcoRIRKpnl 61 (EcoRII)KpnK14I (Kpnl)LpnllMaemMbvlMfelMglflMkil (Hindlll)MW (Alul)Mmefl (Mbol)MnnI (Hindll)Mnol (Hpall)MphI (EcoRII)MscI (Ball)Msp67I (ScrFI)Mspl (HpaH)MthI (Mbol)MvillNaelNarlNasWI (Nael)Ncal (Hinfl)Ndal (Narl)NflAH (Mbol)NfimNgoDHNgoIII (SacII)NgoSI (HaeHI)NlaDffl (SacII)NlaTVNlill (Avail)NmemNmuAH (Avail)NmuFI (Nael)NopI (Sail)NovO (Hinfl)NsiCI (EcoRV)NspBI (AsuII)NspEI (Aval)NspHI (Nspl)NspD (Sdul)NspKI (Avail)NspLJVNspSAH (BstEII)NspWI (Nael)NtaSI (StuI)OtuI (AluOOxaNI (Saul)Pael (SphI)PanI (Xhol)PflMIPgU (NaeDPmaCI

r374



Pmal (PstI)Povl (Bell)Psel (Asul)PssHRhel (Sail)RlelRlul (Nael)RrtiDRshn (CauII)RsrDSacISalAI (Mbol)SailSaol (Nael)Sau3AI (Mbol)SauBMM (Nael)SblBI (Styl)SbrISciAH (PvuII)ScrFISeelSexDSfelSfol (Narl)SgrISinAI (Avail)SinEI (Avail)Sinl (Avail)SLsISlul (Xhol)Snol (ApaLI)SpaXI (SphI)Spin (Tthllll)Ssel (Bell)SsplI (AsuD)SspXISstIV (Bell)SthDI (Kpnl)SthHI (Kpnl)SthLI (Kpnl)StuITaqITfU (TaqI)Tmull (CauII)Tsp45ITteAI (Haeffl)TthHB8I (TaqI)Uba21I (HgiAI)Uur960I (Fnu4HI)Vnel (ApaLI)XbalXcyl (Smal)XmaD (PstI)XmnIXpal (Xhol)YenCI (PstI)ZanI (EcoRU)

PmilPpal (Eco31I)Psp61I (Nael)PsdRhpl (Sail)Rlull (Mbol)RmelRrol (Sail)Rspl (Pvul)Saal (SacII)SacHSalCI (Nael)SainSaulOI (Kpnl)Sau6782I (Mbol)SaulSblCI (Styl)SealScil (Xhol)Scul (Xhol)SecH (Hpall)SfaGUI (Hpall)SfilSfrl (SacU)SgrH (EcoRD)SinBI (Avaff)SinFI (AvaH)SinJI (AvaH)Skal (Nael)SmalSodISpelSpim (Haem)SseH (SacII)Ssp2I (CauH)Ssa (SacI)SthAI (Kpnl)SthEI (Kpnl)SthI (Kpnl)SthMI (Kpnl)StylTaqHTgll (SacII)Tnil (AvaTI)TspEITtel (Tthllll)Ttnl (HaeHI)Uba26I (BsmAI)VanI (Bgll)Vnil (Haem)Xcal (Snal)XholXmamXnil (Pvul)XphI (PstI)YenDI (PstI)

PmU (PmaCI)Ppul (HaeUI)Pspl (Asul)PvulRhpHRlu3I (NlaTV)RiblRsalRspXl (BspHI)SabI (SacU)SacmSalDI (Nrul)SalPI (PstI)Saul2I (Eco31I)Sau96I (Asul)Sbal (PvuH)SboBI (Nrul)Seel (FnuDU)SciNI (Hhal)SdulSeem (Saul)Sfal (HaeUI)Sfll (PstI)Sgal (Xhol)Shyl (SacU)SinCI (Avail)SinGI (Avail)SinMI (Mbol)SkaU (PstI)SnaBISocinSphISsalSsol (EcoRI)Ssp4I (Xhol)Sstn (SacU)SthBI (Kpnl)SthFI (Kpnl)SthJI (Kpnl)SthNI (Kpnl)Sual (HaeUI)TaqXI (EcoRII)Thai (FnuDU)TruU (Mbol)Tspl (Tthllll)TthllllTtrl (Tthllll)Uba441 (ApaLI)Vhal (Haem)VsplXcil (Sail)XhoHXmlAl (Pvul)Xorl (PstI)YenAl (PstI)YenEI (PstI)

Pmyl (PstI)PpuMIPssI (DraU)PvuURhsl (BamHI)Rlu4I (BamHI)Rrhl (Sail)RshI (Pvul)RsrI (EcoRI)SacAI (Nael)SakI (SacU)SalHl (Mbol)SanISau3239I (Xhol)SauAI (Nael)SblAI (Styl)Sbol (SacH)SciAl (BstEE)Scol (SacI)Sdyl (AsuDSexl (Xhol)SfaNISfhl (AvaU)Sgol (Xhol)ShyTISinDI (AvaU)SinHI (Avail)SinMIISlal (Xhol)SnalSpal (Xhol)SplISscISsoU (ScrFI)SsplSstm (Sacffl)SthCI (Kpnl)SthGI (Kpnl)SthKI (Kpnl)StmlSull (HaeUI)Tcel (MboH)TmilTruIIlTspZNI (HaelU)Tthllll lUball (PpuMI)Uba6I (Mlul)VneAI (DraU)Xaml (Sail)XcmlXmal (Smal)Xmll (Pvul)XorU (Pvul)YenBI (PstI)YenI (PstI)

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