Vol. 268, No. 33 The Journal of November 25, 1993

Biological Chemistry Copyright 0 1993 by the American Society for Biochemistry and Molecular Biology, Inc.

428 East Preston St., Baltimore, MD 21202 U.S.A.

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CONTENTS*

COMMUNICATIONS

Optimization of an anti-HIV hairpin ribozyme by in vitro selection. Simpson Joseph and John M. Burke

Specific interaction with rhodopsin is dependent on the y subunit type in a G protein. Oleg Kisselev and Narasim- han Gautam

Identification of bovine glutamate dehydrogenase as an RNA-binding protein. Thomas Preiss, Andrew G. Hall, and Robert N . Lightowlers

A small C-terminal region of the Escherichia coli MalT protein contains the DNA-binding domain. Dominique Vidal-Ingigliardi, Euelyne Richet, Oliuier Danot, and Olivier Raibaud

binding of ADP-ribosylation factor ARFl to phospho- Myristoylation is not required for GTP-dependent

lipids. Michel Franco, Pierre Chardin, Marc Chabre, and Sonia Paris

ADP-ribosylation of rho p21 inhibits lysophosphatidic acid-induced protein tyrosine phosphorylation and phosphatidylinositol 3-kinase activation in cultured Swiss 3T3 cells. Naokazu Kumagai, Narito Morii, Kazuko Fujisawa, Yasuo Nemoto, and Shuh Narumiya

Expression cloning of type 2 angiotensin I1 receptor reveals a unique class of seven-transmembrane recep-

Horiuchi, Hiroyuki Sasamura, Richard E. Pratt, and Victor J. tors. Masashi Mukoyama, Masatoshi Nakajima, Masatsugu

Dzau

Molecular cloning of a novel angiotensin I1 receptor isoform involved in phosphotyrosine phosphatase in- hibition. Yoshikazu Kambayashi, Smriti Bardhan, Kyoko

and Tadashi Inagami Takahashi, Satoshi Tsuzuki, Hiroshi Inui, Takao Hamakubo,

In vivo control of redox potential during protein folding catalyzed by bacterial protein disulfide-isomerase (DsbA). Martina Wunderlich and Rudi Glockhuber

GK* and brain G8, activate muscarinic K+ channel

Jahangir, Yukio Hosoya, A t s w h i Inanobe, Toshiaki Katada, through the same mechanism. Mitsuhiko Yamada, Arshad

and Yoshihisa Kurachi

of chloramphenicol acetyltransferase. J o h n Robben, J a n Carboxyl terminus is essential for intracellular folding

Van der Schueren. and Guido Volckaert

ARTICLES

Phospholipase C activation during elicitation of the oxidative burst in cultured plant cells. Laurent Legendre, Yir G. Yueh, Richard Crain, Nathan Haddock, Peter F. Hein- stein, and Philip S. Low

Sequence of the sodium ion pump methylmalonyl-CoA

and Peter Dimroth decarboxylase from Veillonella parvula. Jon B. Huder

Effect of mutations at active site residues on the activ-

active site-directed irreversible inhibitors. Catherine S. ity of ornithine decarboxylase and its inhibition by

Coleman, Bruce A. Stanley, and Anthony E. Pegg

Cloning of a gene (PSDl) encoding phosphatidylserine decarboxylase from Saccharomyces cerevisiae by com-

J . Clancey, Shao-Chun Chang, and William Dowhan plementation of an Escherichia coli mutant. Constance

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24669

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24683

24692

Eicar (5-ethynyl-1-~-~-ribofuranosylimidazole-4-car- boxamide). A novel potent inhibitor of inosinate dehy- drogenase activity and guanylate biosynthesis. Jan Bal- zarini, Anna Karlsson, Liya Wang, Christina Bohman, Kvetos-

Aerschot, Piet Herdewijn, and Erik De Clercq lava Horska, Ivan Votruba, Arnold Fridland, Arthur Van

Site-directed mutagenesis of rabbit muscle phospho- fructokinase cDNA. Mutations a t glutamine 200 affect

Zhu, Malcolm Byres , Je f f r ey W. Nelson, and Simon H. Chang the allosteric properties of the enzyme. Jauyi Li, Xiaomao

Kinetic mechanism of the DNA-dependent DNA polym- erase activity of human immunodeficiency virus re- verse transcriptase. Jen-Chih Hsieh, Shawn Zinnen, and Paul Modrich

Purification and sequence of rat extracellular super- oxide dismutase B secreted by CS glioma. Jean Willems, A n Zwijsen, Herman Slegers, Stefan Nicolac, Jayaram Betta- dapura, Jos Raymackers, and Thierry Scarcez

Probing the reactivity of the GTP- and GDP-bound conformations of elongation factor Tu in complex with the antibiotic GE2270 A. Pieter H. Anborgh and Andrea Parmeggiani

Biochemical comparisons of the Saccharomyces cere-

Cdc42 GTPase-activating protein domain. Y i Zheng, uisiae Bern2 and Bema proteins. Delineation of a limit

Matthew J . Hart, Katsuhiro Shinjo, Tony Evans, Alan Bender, and Richard A . Cerione

Ca2+-induced folding and aggregation of skeletal mus- cle sarcoplasmic reticulum calsequestrin. The involve-

Keith Dunker, Carla R. Wesson, and William R. Trumble ment of the trifluoperazine-binding site. Zhaoping He, A.

Electron transfer process in milk xanthine dehydro- genase as studied by pulse radiolysis. Kazuo Kobayashi, Miyako Miki, Ken Okamoto, and Takeshi Nishino

Phosphorylation and active ATP hydrolysis are not required for SV40 T antigen hexamer formation. Inga Reynisdottir, Heather E. Lorimer, Paula N . Friedman, Edith H. Wang, and Carol Priues

VLA-4 integrin mediates lymphocyte migration on the inducible endothelial cell ligand VCAM-1 and the ex-

Alejandro Aruffo tracellular matrix ligand fibronectin. Po-Ying Chan and

Fluid membranes with acidic domains activate DnaA, the initiator protein of replication in Escherichia coli. Celina E. Castuma, Elliott Crooke, and Arthur Kornberg

CAMP regulates G-protein ai.2 subunit gene transcrip- tion in polarized LLC-PKI cells by induction of a CCAAT box nuclear binding factor. T. Bernard Kinane, Cheng Shang, Jonathan D. Finder, and Louis Ercolani

Directional regulatory activity of cis-acting elements in the bidirectional al(IV) and a2(1V) collagen gene promoter. Pirkko Heikkila, Raija Soininen, and Karl Trygguason

Characterization of mutations in the b subunit of FIFO ATP synthase in Escherichia coli. Kimberly A. Mc-

and Brian D. Cain Cormick, Gabriele Deckers-Hebestreit, Karlheinz Altendorf,

Inhibition of SDC25 C-domain-induced guanine-nu- cleotide exchange by guanine ring binding domain mu-

Poullet, and Andrea Parmeggiani tants of v-H-ras. Yu- Wen Hwang, Jie-Ming Zhong, Patrick

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in (1993) J. Bid. Chem. 268, 14543-14551, and reprints may be obtained from the editorial office.

iii

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How do "Znl Cyss" proteins distinguish between similar

binding specificity of the Gal4 protein in vitro and in upstream activation sites? Comparison of the DNA-

vivo. Sanjay Vashee, HLlagiang Xu, Stephen A. Johnston, and Thomas Kodadek

rotoxin ShI. Gauin R. Wilcox, Rasmus H. Fogh, and Raymond Refined structure in solution of the sea anemone neu-

S. Norton

Isolation and expression of a cDNA encoding a male- specific rat sulfotransferase that catalyzes activation of N-hydroxy-2-acetylaminofluorene. Kiyoshi Nagata, Shogo Ozawa, Masaaki Miyata, Miki Shimada, Da- Wei Gong, Yasushi Yamazoe, and Ryuichi Kat0

Purification of an acyl-CoA hydrolase from rat intes- tinal microsomes. A candidate acyl-enzyme intermedi- ate in glycerolipid acylation. Richard Lehner and Arnis Kuksis

Interaction of bacterial luciferase with aldehyde sub- strates and inhibitors. Wilson A. Francisco, Husam M. Abu-Soud, Thomas 0. Baldwin, and Frank M. Rawhel

Behavior of Cys-707 (SH1) in myosin associated with ATP hydrolysis revealed with a fluorescent probe linked directly to the sulfur atom. Toshiaki Hiratsuka

Dissociation of platelet-activating factor production and arachidonate release by the endomembrane CaZ+- ATPase inhibitor thapsigargin. Evidence for the in- volvement of a Ca2+-dependent route of priming in the production of lipid mediators by human polymorpho- nuclear leukocytes. Carmen Garcia Rodriguez, Mayte Mon- tero, Jauier Aluarez, Jauier Garcia-Sancho, and Mariano San- chez Crespo

Functional roles of valine 37 and glycine 38 in the mobile loop of porcine cytosolic aspartate aminotrans- ferase. Qian- Wen Pan, Sumio Tanase, Yuhji Fukumoto, Fuji0 Nagashima, Sangkee Rhee, Paul H. Rogers, Arthur Arnone, and Yoshimasa Morino

Multiubiquitin chains linked through lysine 4 8 are abundant in vivo and are competent intermediates in the ubiquitin proteolytic pathway. Steven uan Nocker and Richard D. Vierstra

The anti Z-DNA reactivity of Z-DNA forming se- quences is affected by platinum antitumor drugs. Jose Manuel Perez-Martin, Jose Maria Requena, Dan Craciunescu, Manuel Carlos Ldpez, and Carlos Alonso

ATP and A1 adenosine receptor agonists mobilize intra- cellular calcium and activate K+ and C1- currents in normal and cystic fibrosis airway epithelial cells. Mich- ela Rugolo, Teresa Mastrocola, Cloudia Whdrle, Andrea Rasola, Dieter C. Gruenert, Grouanni Romeo, and Luis J. V. Galietta

Purification and characterization of an enzyme in- volved in oxidative carbon-carbon bond cleavage re- actions in the methionine salvage pathway of Klebsiella pneumoniae. Robert W. Myers, Jonathan W. Wray, Susan Fish, and Robert H. Abeles

enzyme E-1 from the methionine salvage pathway of Appendix. Cloning and sequence of the gene encoding

Klebsiella oxytoca. Ramaswamy Balokrishnan, Michael Frohlich, Peter T. Rahaim, Keith Backman, and R. Rogers Yocum

Reversible association of a 97-kDa protein complex found at the tips of ciliary microtubules with in vitro assembled microtubules. Wen Wang, Kathy Suprenant, and William L. Dentler

binding domain of the orphan nuclear receptor NGFI- The phosphorylation and DNA binding of the DNA-

Milbrandt, and Gordon Guroff B. Yoko Hirata, Kazutoshi Kiuchi, Hao-Chia Chen, Jeffrey

The Schitosaccharomyces pombe mating-type gene mat-Mc encodes a sequence-specific DNA-binding high mobility group box protein. Dennis Dooijes, Marc van de Wetering, &on Knippels, and Hans Cleuers

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Analysis of lipopolysaccharide binding by CD14. The0

Lee, R. J. Uleuitch, and Peter S. Tobias N. Kirkland, Fred Finley, Didier Leturcq, Ann Moriarty, J.-D.

Alterations in the electron transfer chain in mutant strains of Escherichia coli lacking phosphatidyletha- nolamine. Eugenia I. Mileykovskaya and William Dowhan

Lipophilic 1,l-bisphosphonates are potent squalene synthase inhibitors and orally active cholesterol low- ering agents in vivo. Carl P. Ciosek, Jr., David R. Magnin, Thomas W. Harrity, Janette V. H. Logan, John K. Dickson,, Jr., Eric M. Gordon, Kelly A. Hamilton, Kern G. Jolibois, &art K. Kunselman, R. Michael Lawrence, Kasim A. Mookhttar, Lois C. Rich, Dorothy A . Slusarchyk, Richard B. Sulsky, and Scott A . Btller

Alteration of vitronectin. Characterization of changes induced by treatment with urea. Steven V. Bittorf, Eliot C. Williams, and Deane F. Masher

Cytotoxicity of folate-Pseudomonas exotoxin conju-

domain. Christopher P. Leamon, Ira Pastan, and Philip S. gates toward tumor cells. Contribution of translocation

Low

Processivity of mitochondrial DNA polymerase from Drosophila embryos. Effects of reaction conditions and enzyme purity. Andrea J . Wi l l iam, Catherine M. Wernette, and Laurie S. Kaguni

Characterization of wheat germ protein synthesis ini- tiation factor eIF-4C and comparison of eIF-4C. from wheat germ and rabbit reticulocytes. Richard T. T m m e r , Sandra R. Lax, Diane L. Hughes, William C. Merrick, Joanne M. Ravel, and Karen S. Browning

Evidence for kinetically distinct forms of pp60""" with

J. A. Budde different Km values for their protein substrate. Raymond

The interaction of N-ethyl retinamide with plasma ret- inol-binding protein (RBP) and the crystal structure of

Zanotti, Giorgio Malpeli, and Rodolfo Berni the retinoid-RBP complex at 1.9-A resolution. Giuseppe

A novel receptor tyrosine phosphatase-o that is highly expressed in the nervous system. Hai Yan, Albert Gross- man, Hong Wang, Peter D'Eustachio, Keuin Mossie, Jose M . Musacchio, Olli Siluennoinen, and Joseph Schlessinger

by a furin-directed al-antitrypsin variant. Eric D. A n - Inhibition of HIV-1 gpl6O-dependent membrane fusion

derson, Laurel Thomas, Joel S. Hayflick, and Gary Thomas

Three clustered S p l sites are required for efficient transcription of the TATA-less promoter of the gene for insulin-like growth factor-binding protein-2 from the rat. Yues R. Boisclair, Alexandra L. Brown, Stefan0 Casoln, and Matthew M. Rechler

Characterization of the cell adhesion molecule gp24 in Dictyostelium discoideum. Mediation of cell-cell adhe- sion via a Caz+-dependent mechanism. Simuran Kaur Brar and Chi-Hung Siu

Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells. Fu-Sheng Shen, Nabil G. Seidah, and Iris Lindberg

Diversity of intermediate filament structure. Evidence that the alignment of coiled-coil molecules in vimentin is different from that in keratin intermediate filaments. Peter M. Steinert, Lyuben N. Marekou, and David A . D. Parry

Essentiality of the three carboxyl-terminal amino acids of the plasmid RK2 replication initiation protein TrfA for DNA binding and replication activity in Gram- negative bacteria. Joan Lin Cereghino and Donald R. Helinski

Molecular basis of the alcohol dehydrogenase-negative deer mouse. Evidence for deletion of the gene for class I enzyme and identification of a possible new enzyme Class. YOO- WU Zheng, Monica Bey, Hong Liu, and Michael R. Felder

428 East Preston Street, Baltimore, MD 21202, U.S.A. Volume 269 for 1994: Institutions: United States, $790; Foreign, $980. Students/PostdoctoraI The Journal of Biological Chemistry (ISSN 0021-9258) is published weekly by the American Society for Biochemistry and Molecular Biology, Inc.,

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Effects of integration and replication on transcription of the HIV-1 long terminal repeat. Kuan-Teh Jeang, Ben Berkhout, and Bora Dropulic

y-subunit. Tissue specificity produced by alternative Gene structure of human mitochondrial ATP synthase

and Yasuo Kagawa RNA splicing. Chie Matsuda, Hitoshi Endo, Shigeo Ohta,

of nitric oxide synthase in cultured vascular smooth Cyclic AMP-elevating agents induce an inducible type

muscle cells. Synergism with the induction elicited by inflammatory cytokines. Masanobu Koide, Yasuhiro Ka- wahara, Ichiro Nakayama, Terutaka Tsuda, and Mitsuhiro Yokoyama

Post-translational processing of proopiomelanocortin

duced by a POMC-Simian virus 40 large T antigen (POMC) in mouse pituitary melanotroph tumors in-

transgene. Malcolm J. Low, Bin Liu, Gary D. Hammer, Marcel0 Rubinstein, and Richard G. Allen

Dynamic aspects of DNA/protein interactions in the transcriptional initiation complex and the hormone- responsive domains of the phosphoenolpyruvate car- boxykinase promoter in vivo. Steven Faber, Richard M. O’Brien, Enyu Imai, Daryl K. Granner, and Roger Chalkley

transferase active site lysine. Edward G. Niles and Linda Identification of the vaccinia virus mRNA guanyl-

Christen

Impaired biosynthesis of phosphatidylcholine causes a

particles in the Golgi but not in the endoplasmic retic- decrease in the number of very low density lipoprotein

ulum of rat liver. Henkjan J. Verkade, Darren G. Fast, Antonio E. Rusiriol, Douglas G. Scraba, and Dennis E. Vance

An activation-associated ganglioside in rat thymocytes. Keiko Nohara, Tomoharu Sano, and Fuji0 Shiraishi

Palmitoylation is required for signaling functions and

gaertner, David H. Chu, Paul Wilson, Mark J. Levis, and membrane attachment of G and G a . Philip B. Wede-

Henry R. Bourne

Endotoxin induces rapid protein tyrosine phosphoryl- ation in 70Z/3 cells expressing CD14. Jiahuai Hun, Jiing-Dwan Lee, Peter S. Tobias, and Richard J . Uleuitch

Binding of collagen XIV with the dermatan sulfate side chain of decorin. Bernard Font, Elisabeth Aubert-Foucher, Denise Goldschmidt, Denise Eichenberger, and Michel van der Rest

Role of glucokinase and glucose-6-phosphatase in the

by insulin. Nir Barzilai and Lucian0 Rossetti acute and chronic regulation of hepatic glucose fluxes

Enhanced activation of the human histone H2B pro- moter by an Oct-1 variant generated by alternative splicing. Gokul Das and Winship Herr

RNA polymerases IIA and I10 have distinct roles dur- ing transcription from the TATA-less murine dihydro- folate reductase promoter. Mona E. Kang and Michael E. Dahmus

Cloning of a membrane-associated protein which mod-

transporter. Maike Veyhl, Jorg Spangenberg, Bernd Piischel, ifies activity and properties of the Na+-D-glucose co-

Robert Poppe, Carmela Dekel, Giinter Fritzsch, Winfried Haase, and Hermann Koepsell

Identification of the major phosphorylation domain of murine rndrlb P-glycoprotein. Analysis of the protein kinase A and protein kinase C phosphorylation sites. George A. Orr, Edward K.-H. Hun, Paul C. Browne, Edward Nieues, Brigid M. O’Connor, Chia-Ping Huang Yang, and Susan Band Horwitz

Isolation of a protein activator of the clathrin-coated vesicle proton pump. X&-Song Xie, Bill P. Crider, and Dennis K. Stone

Expression of the complement classical pathway by

expression by nerve cells. Philippe Casque, Alexander human glioma in culture. A model for complement

Ischenko, Jocelyne Legoedec, Claire Mauger, Marie-Thirese Schouft, and Marc Fontaine

Purification and characterization of a mitogen-acti-

pus eggs. Boris Sarceuic, Eleanor Erikson, and James L. vated protein kinase tyrosine phosphatase from Xeno-

Muller

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Characterization of the nucleational core complex re- sponsible for mineral induction by growth plate carti- lage matrix vesicles. Licia N. Y. Wu, Takayuki Yoshimori,

Ishikawa, and Roy E. Wuthier Brian R. Genge, Glenn R. Sauer, Thorsten Kirsch, Yoshinori

Angiotensin I1 stimulates the synthesis of angiotensin- ogen in hepatocytes by inhibiting adenylylcyclase ac-

Klett, Ruiner Nobiling, Peter Gierschik, and Eberhard tivity and stabilizing angiotensinogen mRNA. Christoph

Hackenthal

The agrin receptor. Localization in the postsynaptic

the acetylcholine receptor. Jianyi Ma, Mary A. Nastuk, membrane, interaction with agrin, and relationship to

Beth A. McKechnie, and Justin R. Fallon

Chloroplast-encoded protein as a subunit of acetyl-coA carboxylase in pea plant. Yukiko Sasaki, Kazuhiko Haka- mada, Yukiko Suama, Yukio Nagana, Iwao Furusawa, and Ryuichi Matsuno

Identification of a secondary FcyRI binding site within

anne Chappel, David E. Isenman, R. Oomen, Yuan Yuan Xu, a genetically engineered human IgG antibody. M. Suz-

and Michel H. Klein

Raf perinuclear translocation via a protein kinase C- 1,25-Dihydroxyvitamin Ds activates Raf kinase and

dependent pathway. Trevor W. Lissoos, David W. A. Beno, and Bernard H. Davis

Independent regulation of two cytoplasmic processing stages of the intermediate filament-associated protein

A. Resing, Nadia Al-Alawi, Cheryl Blomquist, Phil Fleckman, filaggrin and role of Caz+ in the second stage. Katheryn

and Beverly A. Dale

Phosphorylation of synthetic peptides containing Tyr- Met-X-Met motifs by nonreceptor tyrosine kinases in

Duane A . Hinds, Allan R. Coldberg, and W . Todd Miller vitro. Pilar Garcia, Steven E. Shoelson, Shaji T. George,

Molecular cloning of two abundant protein tyrosine kinases in Torpedo electric organ that associate with the acetylcholine receptor. Sheridan L. Swope and Richard L. Huganir

substitutions for glycine in the a 2 (I) collagen chain. A Two additional cases of osteogenesis imperfecta with

type. Qin Wang, Bonnie M. Orrison, and Joan C. Marini regional model relating mutation location with pheno-

Movement of apolipoprotein B into the lumen of micro- somes from hepatocytes is disrupted in membranes en- riched in phosphatidylmonomethylethanolamine. Anto- nio E. Rusiriol, Edmond Y. W. Chan, and Jean E. Vance

Characterization of the lectin from the bulbs of Eran-

synthesis. M. Arun Kumar, David E. Timm, Kenneth E. this hyernalis (winter aconite) as an inhibitor of protein

Neet, Whyte G. Owen, Willy J . Peumans, and A. Gururaj Rao

Purification of a 110-kDa phosphoinositide phospholi- pase C that is activated by G-protein By-subunits. Jon- athan L. Blank, Karen Shaw, Annette H. Ross, and John H. Exton

Function of the GrpE heat shock protein in bidirec- tional unwinding and replication from the origin of phage X. Claire Wyman, Christos Vasilikiotis, Debbie Ang, Costa Georgopoulos, and Harrison Echols

Osteogenic protein-1 regulates L1 and neural cell adhe- sion molecule gene expression in neural cells. George Perides, Guang Hu, David C. Rueger, and Michael E. Charness

Loading dependence of inositol 1,4,5-trisphosphate-in- duced Ca2+ release in the clonal cell line A7r5. Impli- cations for the mechanism of quantal Ca2+ release. J a n B. Parys, Ludwig Missiaen, Humbert De Smedt, and Rik Casteels

Calmodulin-cardiac troponin C chimera. Effects of do- main exchange on calcium binding and enzyme activa- tion. Samuel E. George, Zenghua Su, Daju Fan, and Anthony R. Means

The role of nucleotides conserved in eukaryotic initia-

Harold J . Drabkin, Bernhard Helk, and Uttam L. RajBhandary tor methionine tRNAs in initiation of protein synthesis.

ity detection of protein-DNA interactions. Application Stable fluorescent dye-DNA complexes in high sensitiv-

to heat shock transcription factor. Hays S. Rye, Becky L. Drees, Hillary C. M. Nelson, and Alexander N . Glazer

vii

25239 Spatial resolution of fodrin proteolysis in postischemic brain. Takaomi C. Saido, Masayuki Yokota, Shunsuke Nagao, Ikuya Yarnaura, Eiichi Tani, Takahide Tsuchiya, Koichi Su- zuki, and Seiichi Kawashirna

25244 I n vitro chamber specification during embryonic stem cell cardiogenesis. Expression of the ventricular myo- sin light chain-2 gene is independent of heart tube formation. Wanda C. Miller-Hance, Monique LaCorbiere, Stephen J. Fuller, Sylvia M. Evans, Gary Lyons, Christel Schmidt, Jeffrey Robbins, and Kenneth R. Chien

25253 Characterization and chromosomal mapping of the hu-

Masakazu Hirata, Akrra Kakizuka, Toshihrko Eki, Kazuo man thromboxane A. receptor gene. Rolf M. Niising,

Ozawa, and Shuh Narumiya

25260 Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identifica- tion of protein domains that participate in Na,K-ATP- ase and H,K-ATPase subunit assembly. Vol. 268 (1993) 14342-14347. Cara J. Gottardr and Mrchael J. Caplan

xiii Cumulative author index. Numbers 1 through 33.

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Vol. 268, No. 33 The Journal of Biological Chemistry November 25, 1993

CONTENTS Arranged by

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

24832 Lipophilic 1,l-bisphosphonates are potent squalene synthase inhibitors and orally active cholesterol low- ering agents in vivo. Carl P. Ciosek, Jr., David R. Magnin,

Jr., Eric M. Gordon, Kelly A . Hamilton, Kern G. Jolibois, Lori Thomas W. Harrity, Janette V. H. Logan, John K. Dickson,

K. Kunselman, R. Michael Lawrence, Kasim A. Mookhtiar, Lois C. Rich, Dorothy A . Slusarchyk, Richard B. Sulsky, and Scott A. Biller

24997 An activation-associated ganglioside in rat thymocytes. Keiko Nohara, Tomoharu Sano, and Fuji0 Shiraishi

25118 Chloroplast-encoded protein as a subunit of acetyl-coA carboxylase in pea plant. Yukiko Sasaki, Kazuhiko Haka- mada, Yukiko Suama, Yukio Nagano, Iwao Furusawa, and Ryuichi Matsuno

CELL BIOLOGY AND METABOLISM 024519 Specific interaction with rhodopsin is dependent on the

y subunit type in a G protein. Oleg Kisseleu and Narasim- han Gautam

024523 Identification of bovine glutamate dehydrogenase as an RNA-binding protein. Thomas Preiss, Andrew G. Hall, and Robert N . Lightowlers

-24531 Myristoylation is not required for GTP-dependent binding of ADP-ribosylation factor ARFl to phospho-

Sonia Paris lipids. Michel Franco, Pierre Chardin, Marc Chabre, and

024535 ADP-ribosylation of rho p21 inhibits lysophosphatidic acid-induced protein tyrosine phosphorylation and phosphatidylinositol 3-kinase activation in cultured Swiss 3T3 cells. Naokazu Kumagai, Narito Morii, Kazuko Fujisawa, Yasuo Nemoto, and Shuh Narumiya

-24543 Molecular cloning of a novel angiotensin I1 receptor

hibition. Yoshikazu Kambayashi, Smriti Bardhan, Kyoko isoform involved in phosphotyrosine phosphatase in-

Takahashi, Satoshi Tsuzuki, Hiroshi Inui, Takao Hamakubo, and Tadashi Inagami

-24551 GK* and brain Got activate muscarinic K+ channel

Jahangir, Yukio Hosoya, Atsushi Inanobe, Toshiaki Katada, through the same mechanism. Mitsuhiko Yamada, Arshad

and Yoshihlsa Kurachi

24559 Phospholipase C activation during elicitation of the oxidative burst in cultured plant cells. Laurent Legendye, Yir G. Yueh, Richard Crain, Nathan Haddock, Peter F. Hern- stein, and Philip S. Low

24591 Eicar (5-ethynyl-l-~-~-ribofuranosylimidazole-4-car- boxamide). A novel potent inhibitor of inosinate dehy- drogenase activity and guanylate biosynthesis. J a n Bal- zarini, Anna Karlsson, Liya Wang, Christina Bohman, Kuetos-

Aerschot, Piet Herdewijn, and Erik De Clercq lava Horska, Ivan Votruba, Arnold Fridland, Arthur Van

24655 VLA-4 integrin mediates lymphocyte migration on the inducible endothelial cell ligand VCAM-1 and the ex- tracellular matrix ligand fibronectin. Po- Yrng Chon and Alejandro Aruffo

24692 Inhibition of SDC25 C-domain-induced guanine-nu- cleotide exchange by guanine ring binding domain mu- tants of v-H-ras. Yu- Wen Hwang, Jie-Ming Zhong, Patrick Poullet, and Andrea Parmeggiani

24751 Dissociation of platelet-activating factor production and arachidonate release by the endomembrane CaZ+- ATPase inhibitor thapsigargin. Evidence for the in- volvement of a Ca2+-dependent route of priming in the production of lipid mediators by human polymorpho- nuclear leukocytes. Carmen Garcia Rodriguez, Mayte Mon- tero, Javier Aluarez, Jauier Garcia-Sancho, and Mariano San- chez Crespo

Subject Categories

24766 Multiubiquitin chains linked through lysine 48 are abundant in vivo and are competent intermediates in

Richard D. Vierstra the ubiquitin proteolytic pathway. Steven van Nocker and

24796 Reversible association of a 97-kDa protein complex found at the tips of ciliary microtubules with in vitro assembled microtubules. Wen Wang, Kathy Suprenant, and William L. Dentler

24808 The phosphorylation and DNA binding of the DNA- binding domain of the orphan nuclear receptor NGFI- B. Yoko Hirata, Kazutoshi Kiuchi, Hao-Chia Chen, Jeffrey Milbrandt, and Gordon Guroff

24818 Analysis of lipopolysaccharide binding by CD14. The0 N . Kirkland, Fred Finley, Didier Leturcq, A n n Moriarty, J.-D. Lee, R. J . Uleuitch, and Peter S. Tobias

24847 Cytotoxicity of folate-Pseudomonas exotoxin conju- gates toward tumor cells. Contribution of translocation domain. Christopher P. Leamon, Ira Pastan, and Philip S. Low

24880 A novel receptor tyrosine phosphatase-o that is highly expressed in the nervous system. Hai Yan, Albert Gross- man, Hong Wang, Peter D’Eustachio, Kevin Mossie, Jose M. Musacchio, Olli Siluennoinen, and Joseph Schlessinger

24887 Inhibition of HIV-1 gpl6O-dependent membrane fusion by a furin-directed q-antitrypsin variant. Eric D. A n - derson, Laurel Thomas, Joel S. Hayflick, and Gary Thomas

24910 Biosynthesis of the prohormone convertase PC2 in

Fu-Sheng Shen, Nabil G. Seidah, and Iris Lindberg Chinese hamster ovary cells and in rat insulinoma cells.

24959 Cyclic AMP-elevating agents induce an inducible type of nitric oxide synthase in cultured vascular smooth muscle cells. Synergism with the induction elicited by inflammatory cytokines. Masanobu Koide, Yasuhiro Ka- wahara, Ichiro Nakayama, Terutaka Tsuda, and Mitsuhiro Yokoyama

24990 Impaired biosynthesis of phosphatidylcholine causes a decrease in the number of very low density lipoprotein particles in the Golgi but not in the endoplasmic retic-

Antonio E. Rusijlol, Douglas G. Scraba, and Dennis E. Vance ulum of rat liver. Henkjan J . Verkade, Darren G. Fast,

25001 Palmitoylation is required for signaling functions and

gaertner, David H. Chu, Paul Wilson, Mark J . Levls, and membrane attachment of G and G&. Philip B. Wede-

Henry R. Bourne

25009 Endotoxin induces rapid protein tyrosine phosphoryl-

Jiing-Dwan Lee, Peter s. Tobias, and Richard J . Uleuitch ation in 70Z/3 cells expressing CD14. Jiahuai Hun,

25019 Role of glucokinase and glucose-6-phosphatase in the

by insulin. Nir Barzilai and Lucian0 Rossetti acute and chronic regulation of hepatic glucose fluxes

25041 Cloning of a membrane-associated protein which mod- ifies activity and properties of the Na+-D-glucose CO- transporter. Maike Veyhl, JOrg Spangenberg, Bernd PuSchel, Robert Poppe, Carmela Dekel, Gunter Fritzsch, Winfried H m e , and Hermann Koepsell

25108 The agrin receptor. Localization in the postsynaptic membrane, interaction with agrin, and relationship to the acetylcholine receptor. Jianyi Ma, Mary A. Nastuk, Beth A. McKechnie, and Justin R. Fallon

25132 1,25-Dihydroxyvitamin D1 activates Raf kinase and Raf perinuclear translocation via a protein kinase C-

and Bernard H. Davis dependent pathway. Trevor W . Lissoos, David W. A . Beno,

25139 Independent regulation of two cytoplasmic processing stages of the intermediate filament-associated protein filaggrin and role of CaZ+ in the second stage. K a t h r y n A. Resing, Nadra Al-Alawr, Cheryl Blomquist, Phrl Fleckman, and Beverly A. Dale

Indicates a Communication

viii

ix

25168

25184

25206

25239

25260

24572

24599

24607

24614

24642

24720

24726

24734

24785

24792

24868

25075

Movement of apolipoprotein B into the lumen of micro- somes from hepatocytes is disrupted in membranes en- riched in phosphatidylmonomethylethanolamine. Anto- nio E. Rusiriol, Edmond Y. W. Chan, and Jean E. Vance

Purification of a 110-kDa phosphoinositide phospholi- pase C that is activated by G-protein By-subunits. Jon- athan L. Blank, Karen Shaw, Annette H. Ross, and John H. Exton

Loading dependence of inositol 1,4,5-trisphosphate-in- duced CaZ+ release in the clonal cell line A7r5. Impli- cations for the mechanism of quantal CaZ+ release. Jan B. Parys, Ludwig Missiaen, Humbert De Smedt, and Rik Casteels

brain. Takaomi C. Saido, Masayuki Yokota, Shunsuke Nagao, Spatial resolution of fodrin proteolysis in postischemic

Ikuya Yamnura, Eiichi Tani, Takahide Tsuchiya, Koichi Su- zuki, and Seiichi Kawashima

of multisubunit ion-transporting ATPases. Identifica- Molecular requirements for the cell-surface expression

tion of protein domains that participate in Na,K-ATP- ase andH,K-ATPase subunit assembly. Vol. 268 (1993) 14342-14347. Cara J . Gottardi and Michael J . Caplan

ENZYMOLOGY Effect of mutations at active site residues on the activ- ity of ornithine decarboxylase and its inhibition by active site-directed irreversible inhibitors. Catherine S. Coleman, B r u e A . Stanley, and Anthony E. Pegg

Site-directed mutagenesis of rabbit muscle phospho- fructokinase cDNA. Mutations a t glutamine 200 affect the allosteric properties of the enzyme. Jauyi Li, Xiaomao Zhu, Malcolm Byrnes, Jeffrey W. Nelson, and Simon H. Chang

Kinetic mechanism of the DNA-dependent DNA polym- erase activity of human immunodeficiency virus re- verse transcriptase. Jen-Chih Hsieh, Shawn Zinnen, and Paul Modrich

Purification and sequence of rat extracellular super- oxide dismutase B secreted by CS glioma. Jean Willems, A n Zwijsen, Herman Slegers, Stefan Nicolac, Jayaram Betta- dapura, Jos Raymockers, and Thierry Scarcez

Electron transfer process in milk xanthine dehydro- genase as studied by pulse radiolysis. Kazw Kobayashi, Miyako Miki, Ken Okamoto, and Takeshi Nishino

specific rat sulfotransferase that catalyzes activation Isolation and expression of a cDNA encoding a male-

of N-hydroxy-2-acetylaminofluorene. Kiyoshi Nagata, Shogo Ozawa, Masaaki Miyata, Miki Shimada, Da- Wei Gong, Yasushi Yamazoe, and Ryuichi Kat0

Purification of an acyl-CoA hydrolase from rat intes- tinal microsomes. A candidate acyl-enzyme intermedi- ate in glycerolipid acylation. Richard Lehner and Arnis Kuksis

Interaction of bacterial luciferase with aldehyde sub- strates and inhibitors. Wilson A. Francisco, Husam M. Abu-Soud, Thomas 0. Baldwin, and Frank M. Raushel

Purification and characterization of an enzyme in- volved in oxidative carbon-carbon bond cleavage re- actions in the methionine salvage pathway of Klebsiella pneumoniae. Robert W. Myers, Jonathan W. Wray, Susan Fish, and Robert H. Abeles

Appendix. Cloning and sequence of the gene encoding

Klebsiella oxytoca. Ramaswamy Balakrishnan, Michael enzyme E-1 from the methionine salvage pathway of

Frohlich, Peter T. Rahaim, Keith Backmnn, and R. Rogers Yocum

Evidence for kinetically distinct forms of pp60""" with

J. A. Budde different K , values for their protein substrate. Raymond

Purification and characterization of a mitogen-acti- vated protein kinase tyrosine phosphatase from Xeno- pus eggs. Boris Sarceuic, Eleanor Erikson, and James L. Maller

25146 Phosphorylation of synthetic peptides containing Tyr- Met-X-Met motifs by nonreceptor tyrosine kinases in

Dunne A. Hinds, Allan R. Goldberg, and W. Todd Miller vitro. Pilar Garcia, Steven E. Shoelson, Shaji T. George,

25152 Molecular cloning of two abundant protein tyrosine kinases in Torpedo electric organ that associate with the acetylcholine receptor. Sheridan L. Swope and Richard L. Huganir

24564

24580

24683

24779

24824

24902

24950

25063

MEMBRANES AND BIOENERGETICS

decarboxylase from Veillonella parvula. Jon B. Huder Sequence of the sodium ion pump methylmalonyl-CoA

and Peter Dimroth

Cloning of a gene ( P S D l ) encoding phosphatidylserine decarboxylase from Saccharomyces cerevisiae by com- plementation of an Escherichia coli mutant. Constance J . Clancey, Shm-Chun Chang, and William Dowhan

Characterization of mutations in the b subunit of FIFo ATP synthase in Escherichia coli. Kimberly A. Mc-

and Brian D. Cain Cormick, Gabriele Deckers-Hebestreit, Karlheinz Altendorf,

ATP and A, adenosine receptor a onists mobilize intra- cellular calcium and activate K f and C1- currents in

ela Rugolo, Teresa Mastrocola, Claudia W h d e , Andrea Rasola, normal and cystic fibrosis airway epithelial cells. Mich-

Dieter C. Gruenert, Gwuanni Romeo, and Luis J. V. Galietta

Alterations in the electron transfer chain in mutant strains of Escherichia coli lacking phosphatidyletha- nolamine. Eugenia I. Mileykouskaya and William Dowhan

Dictyostelium discoideum. Mediation of cell-cell adhe- Characterization of the cell adhesion molecule gp24 in

sion via a Ca2+-dependent mechanism. Simuran Kaur Brar and Chi-Hung Siu

y-subunit. Tissue specificity produced by alternative Gene structure of human mitochondrial ATP synthase

and Yasuo Kagawa RNA splicing. Chie Matsuda, Hitoshi Endo, Shigeo Ohta,

Isolation of a protein activator of the clathrin-coated vesicle proton pump. Xiao-Song Xie, Bill P. Crider, and Dennis K. Stone

25084 Characterization of the nucleational core complex re-

lage matrix vesicles. Licia N. Y. Wu, Takayuki Yoshimori, sponsible for mineral induction by growth plate carti-

Ishikawa, and Roy E. Wuthier Brian R. Genge, Glenn R. Sauer, Thorsten Kirsch, Yoshinori

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

-24515 Optimization of an anti-HIV hairpin ribozyme by in vitro selection. Simpson Joseph and John M. Burke

024527 A small C-terminal region of the Escherichia coli MalT protein contains the DNA-binding domain. Dominique

Raibaud Vidal-Ingigliardi, Euelyne Richet, Oliuier Danot, and Oliuier

024539 Expression cloning of type 2 angiotensin I1 receptor reveals a unique class of seven-transmembrane recep- tors. Masashi Mukoyama, Masatoshi Nakajima, Masatsugu Horiuchi, Hiroyuki Sasamura, Richard E. Pratt, and Victor J . Dzau

24622 Probing the reactivity of the GTP- and GDP-bound conformations of elongation factor Tu in complex with

Parmeggiani the antibiotic GE2270 A. Pieter H. Anhrgh and Andrea

24647 Phosphorylation and active ATP hydrolysis are not

Reynisdottir, Heather E. Lorimer, Paula N. Friedman, Edith required for SV40 T antigen hexamer formation. Inga

H. Wang, and Carol Priues

24665 Fluid membranes with acidic domains activate DnaA, the initiator protein of replication in Escherichia coli. Celina E. Castuma, Elliott Crooke, and Arthur Kornberg

24669 CAMP regulates G-protein ai.2 subunit gene transcrip- tion in polarized LLC-PK1 cells by induction of a CCAAT box nuclear binding factor. T. Bernard Kinane, Cheng Shang, Jonathan D. Finder, and Louis Ercolani

Indicates a Communication

X

24677

24699

24774

24813

24855

24863

24892

24926

24933

24940

24967

24976

24986

25026

25033

25068

25095

Directional regulatory activity of cis-acting elements

promoter. Pirkko Heikkila, Raija Soininen, and Karl in the bidirectional al(1V) and a2(IV) collagen gene

Tryggvason

How do "Znl Cy%" proteins distinguish between similar upstream activation sites? Comparison of the DNA- binding specificity of the Gal4 protein in vitro and in uiuo. Sanjay Vashee, Huagiang Xu, Stephen A. Johnston, and Thomas Kodadek

quences is affected by platinum antitumor drugs. Jose The anti Z-DNA reactivity of Z-DNA forming se-

Manuel Carlos Lopez, and Carlos Alonso Manuel Perez-Martin, Jose Maria Requena, Dan Craciunescu,

The Schizosaccharomyces pombe mating-type gene mat-Mc encodes a sequence-specific DNA-binding high mobility group box protein. Dennis Dooijes, Marc van de Wetering, Lion Knippels, and Hans Clevers

Drosophila embryos. Effects of reaction conditions and Processivity of mitochondrial DNA polymerase from

enzyme purity. Andrea J . Williams, Catherine M. Wernette, and Laurie S. Kaguni

Characterization of wheat germ protein synthesis ini- tiation factor eIF-4C and comparison of eIF-4C from wheat germ and rabbit reticulocytes. Richard T. Timmer, Sandra R. Lax, Diane L. Hughes, William C. Merrick, Joanne M. Ravel, and Karen S. Browning

Three clustered S p l sites are required for efficient transcription of the TATA-less promoter of the gene for insulin-like growth factor-binding protein-2 from the rat. Yues R. Boisclair, Alexandra L. Brown, Stefan0 Casola, and Matthew M. Rechler

of the plasmid RK2 replication initiation protein TrfA Essentiality of the three carboxyl-terminal amino acids

for DNA binding and replication activity in Gram- negative bacteria. Joan L i n Cereghino and Donald R. Helinski

Molecular basis of the alcohol dehydrogenase-negative deer mouse. Evidence for deletion of the gene for class I enzyme and identification of a possible new enzyme

Felder class. Yao- W u Zheng, Monica Bey, Hang Liu, and Michael R.

Effects of integration and replication on transcription of the HIV-1 long terminal repeat. K w n - T e h Jeang, Ben Berkhout, and Bora Dropulic

Post-translational processing of proopiomelanocortin

duced by a POMC-Simian virus 40 large T antigen (POMC) in mouse pituitary melanotroph tumors in-

transgene. Malcolm J. Low, Bin Liu, Gary D. Hammer, Marcel0 Rubinstein, and Richard G. Allen

transcriptional initiation complex and the hormone- Dynamic aspects of DNA/protein interactions in the

boxykinase promoter in uiuo. Steven Faber, Richard M. responsive domains of the phosphoenolpyruvate car-

O'Brien, Enyu Imai, Daryl K. Granner, and Roger Chalkley

Identification of the vaccinia virus mRNA guanyl- transferase active site lysine. Edward G. Niles and Linda Christen

Enhanced activation of the human histone H2B pro- moter by an Oct-1 variant generated by alternative splicing. Gokul Das and Winship Herr

RNA polymerases IIA and I10 have distinct roles dur- ing transcription from the TATA-less murine dihydro- folate reductase promoter. Mona E. Kang and Michael E. Dahmus

Expression of the complement classical pathway by human glioma in culture. A model for complement expression by nerve cells. Philippe Gasque, Alexander Ischenko, Jocelyne Legoedec, Claire Mauger, Marie-Thirese Schouft, and Marc Fontarne

Angiotensin I1 stimulates the synthesis of angiotensin- ogen in hepatocytes by inhibiting adenylylcyclase ac- tivity and stabilizing angiotensinogen.mRNA. Christoph Klett, Ruiner Nobrlrng, Peter Grerschlk, and Eberhard Hackenthal

25162

25192

25197

25221

25229

25244

25253

'24547

'24555

24629

24635

24707

24742

24758

24838

24873

24916

25015

Two additional cases of osteogenesis imperfecta with substitutions for glycine in the a 2 (I) collagen chain. A regional model relating mutation location with pheno- type. Qin Wang, Bonnie M. Orrison, and Joan C. Marini

Function of the GrpE heat shock protein in bidirec- tional unwinding and replication from the origin of phage X. Claire Wyman, Christos Vasilikiotis, Debbie Ang, Costa Georgopoulos, and Harrison Echols

Osteogenic protein-1 regulates L1 and neural cell adhe-

Perides, Guang Hu, David C. Rueger, and Michael E. Charness sion molecule gene expression in neural cells. George

The role of nucleotides conserved in eukaryotic initia-

Harold J. Drabkin, Bernhard Helk, and Uttam L. RajBhandary tor methionine tRNAs in initiation of protein synthesis.

Stable fluorescent dye-DNA complexes in high sensitiv-

to heat shock transcription factor. Hays S. Rye, Becky L. ity detection of protein-DNA interactions. Application

Drees, Hillary C. M. Nelson, and Alexander N. Glazer

In vitro chamber specification during embryonic stem cell cardiogenesis. Expression of the ventricular myo- sin light chain-2 gene is independent of heart tube

Stephen J. Fuller, Sylvia M. Evans, Gary Lyons, Christel formation. Wanda C. Miller-Hance, Monique LaCorbiere,

Schmidt, Jeffrey Robbins, and Kenneth R. Chien

Characterization and chromosomal mapping of the hu- man thromboxane Az receptor gene. Rolf M. Ntising, Masakazu Hirata, Akira Kakrzuka, Toshihiko Eki, Kazuo Ozawa, and Shuh Narumiya

PROTEIN CHEMISTRY AND STRUCTURE In uiuo control of redox potential during protein folding catalyzed by bacterial protein disulfide-isomerase (DsbA). Martina Wunderlich and Rudi Glockshuber

Carboxyl terminus is essential for intracellular folding of chloramphenicol acetyltransferase. J o h n Robben, Jan Van der Schueren, and Guido Volckaert

Biochemical comparisons of the Saccharomyces cere- uisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. Yi Zheng, Matthew J. Hart, Katsuhiro Shinjo, Tony Evans, Alan Bender, and Richard A. Cerione

Ca2+-induced folding and aggregation of skeletal mus- cle sarcoplasmic reticulum calsequestrin. The involve-

Keith Dunker, Carla R. Wesson, and William R. Trumble ment of the trifluoperazine-binding site. Zhaoping He, A.

Refined structure in solution of the sea anemone neu- rotoxin ShI. Gavin R. Wilcox, Rasmus H. Fogh, and Raymond S. Norton

Behavior of Cys-707 (SH1) in myosin associated with

linked directly to the sulfur atom. Toshiaki Hiratsuka ATP hydrolysis revealed with a fluorescent probe

Functional roles of valine 37 and glycine 38 in the mobile loop of porcine cytosolic aspartate aminotrans- ferase. Qian- Wen Pan, Sumio Tanase, Yuhji Fukumoto, Fuji0 Nagashima, Sangkee Rhee, Paul H. Rogers, Arthur Arnone, and Yoshimasa Morino

Alteration of vitronectin. Characterization of changes induced by treatment with urea. Steven V. Bittorf, Eliot C. Williams, and Deane F. Masher

The interaction of N-ethyl retinamide with plasma ret- inol-binding protein (RBP) and the crystal structure of

Zanotti, Giorgio Malpeli, and Rodolfo Berni the retinoid-RBP complex at 1.9-A resolution. Giuseppe

Diversity of intermediate filament structure. Evidence that the alignment of coiled-coil molecules in vimentin

Peter M. Steinert, Lyuben N. Marekov, and David A. D. Parry is different from that in keratin intermediate filaments.

Binding of collagen XIV with the dermatan sulfate side chain of decorin. Bernard Font, Elisabeth Aubert-Foucher, Denise Goldschmidt, Denise Elchenberger, and Michel van der Rest

Indicates a Communication

xi

25054 Identification of the major phosphorylation domain of murine mdr l b P-glycoprotein. Analysis of the protein kinase A and protein kinase C phosphorylation sites. George A. Orr, Edward R-H. Nan, Paul C. Browne, Edward Nieves, Bri id M. O’Connor, Chin-Ping Huang Yang, and Susan BancfHorwitz

25124 Identification of a secondary FcyRI binding site within a genetically engineered human I G antibody. M. Suz- anne c,hap el, ~ u u d E Isenman, R. Jomen , Yuan Yuan xu , and McchefH. Klein ‘

25176 Characterization of the lectin from the bulbs of Eran- this hyernalis (winter aconite) as an inhibitor of protein synthesis. M. Arun Kumar, David E. Timm, Kenneth E. Neet, Whyte G. Owen, Willy J. Peumans, and A. Gururaj Rao

25213 Calmodulin-cardiac troponin C chimera. Effects of do- main exchange on calcium binding and enzyme activa- tion. Samuel E. George, Zenghua Su, Duju Fan, and Anthony R. Means

Abeles, Robert H., 24785 Abu-Soud, Husam M., 24734 AI-Alawi, Nadia, 25139 Allen, Richard G., 24967 Alonso, Carlos, 24774 Altendorf, Karlheinz, 24683 Alvarez, Javier, 24751 Anborgh, Pieter H., 24622 Anderson, Eric D., 24887 Ang, Debbie, 25192 Arnone, Arthur, 24758

Aubert-Foucher, Elisabeth, Aruffo, Alejandro, 24655

Backman, Keith, 24792 Balakrishnan, Ramaswamy,

Balzarini, Jan, 24591 Baldwin, Thomas O., 24734

Bardhan, Smriti, 24543 Barzilai, Nir, 25019 Bender, Alan, 24629 Beno, David W. A,, 25132 Berkhout, Ben, 24940 Berni, Rodolfo, 24873 Bettadapura, Jayaram, 24614 Bey, Monica, 24933

Bittorf, Steven V., 24838 Biller, Scott A,, 24832

Blank, Jonathan L., 25184 Blomquist, Cheryl, 25139 Bohman, Christina, 24591 Boisclair, Yves R., 24892

Brar, Simuran Kaur, 24902 Bourne, Henry R., 25001

Brown, Alexandra L., 24892 Browne, Paul C., 25054 Browning, Karen S., 24863 Budde, Raymond J. A,, 24868

Byrnes, Malcolm, 24599 Burke, John M., 24515

Cain, Brian D., 24683 Caplan, Michael J., 25260 Casola, Stefano, 24892 Casteels, Rik, 25206

Cereghino, Joan Lin, 24926 Castuma, Celina E., 24665

Cerione, Richard A,, 24629 Chabre, Marc, 24531 Chalkley, Roger, 24976 Chan, Edmond Y. W., 25168 Chan, Po-Ying, 24655 Chang, Shao-Chun, 24580 Chang, Simon H., 24599 Chappel, M. Suzanne, 25124 Chardin, Pierre, 24531 Charness, Michael E., 25197

Chien, Kenneth R., 25244 Chen, Hao-Chia, 24808

Christen, Linda, 24986 Chu, David H., 25001 Ciosek, Carl P., Jr., 24832 Clancey, Constance J., 24580 Clevers, Hans, 24813 Coleman, Catherine S., 24572 Craciunescu, Dan, 24774 Crain, Richard, 24559 Crider, Bill P., 25063

25015

24792

Crooke, Elliott, 24665

Dahmus, Michael E., 25033 Dale, Beverly A,, 25139 Danot, Olivier, 24527 Das, Gokul, 25026 Davis, Bernard H., 25132 Deckers-Hebestreit, Gabriele,

De Clercq, Erik, 24591 Dekel, Carmela, 25041 Dentler, William L., 24796 De Smedt, Humbert, 25206 D’Eustachio, Peter, 24880 Dickson, John K., Jr., 24832 Dimroth, Peter, 24564 Dooijes, Dennis, 24813 Dowhan, William, 24580,

Drabkin, Harold J., 25221 Drees, Becky L., 25229 Dropulic, Boro, 24940 Dunker, A. Keith, 24635 Dzau, Victor J., 24539

Eichenberger, Denise, 25015 Echols, Harrison, 25192

Eki, Toshihiko, 25253 Endo, Hitoshi, 24950 Ercolani, Louis, 24669 Erikson, Eleanor, 25075 Evans, Sylvia M., 25244 Evans, Tony, 24629 Exton, John H., 25184

Faber, Steven, 24976 Fallon, Justin R., 25108 Fan, Daju, 25213 Fast, Darren G., 24990 Felder, Michael R., 24933 Finder, Jonathan D., 24669

Fish, Susan, 24785 Finley, Fred, 24818

Fleckman, Phil, 25139 Fogh, Rasmus H., 24707 Font, Bernard, 25015 Fontaine, Marc, 25068 Francisco, Wilson A,, 24734 Franco, Michel, 24531 Fridland, Arnold, 24591

Fritzsch, Gunter, 25041 Friedman, Paula N., 24647

Frohlich, Michael, 24792 Fujisawa, Kazuko, 24535 Fukumoto, Yuhji, 24758 Fuller, Stephen J., 25244 Furusawa, Iwao, 25118

Galietta, Luis J. V., 24779 Garcia, Pilar, 25146 Garcia-Sancho, Javier, 24751 Gasque, Philippe, 25068 Gautam, Narasimhan, 24519 Genge, Brian R., 25084

George, Shaji T., 25146 George, Samuel E., 25213

Georgopoulos, Costa, 25192

Glazer, Alexander N., 25229 Gierschik, Peter, 25095

Glockshuber, Rudi, 24547 Goldberg, Allan R., 25146

24683

24824

AUTHOR INDEX

Goldschmidt, Denise, 25015 Gong, Da-Wei, 24720 Gordon, Eric M., 24832 Gottardi, Cara J., 25260 Granner, Daryl K., 24976

Gruenert, Dieter C., 24779 Grossman, Albert, 24880

Guroff, Gordon, 24808

Haase, Winfried, 25041 Hackenthal, Eberhard, 25095 Haddock, Nathan, 24559 Hakamada, Kazuhiko, 25118 Hall, Andrew G., 24523 Hamakubo, Takao, 24543 Hamilton, Kelly A,, 24832 Hammer, Gary D., 24967 Han, Edward K.-H., 25054 Han, Jiahuai, 25009 Harrity, Thomas W., 24832 Hart, Matthew J., 24629 Hayflick, Joel S., 24887 He, Zhaoping, 24635 Heikkila, Pirkko, 24677 Heinstein, Peter F., 24559 Helinski, Donald R., 24926 Helk, Bernhard, 25221 Herdewijn, Piet, 24591 Herr, Winship, 25026 Hinds, Duane A,, 25146 Hirata, Masakazu, 25253

Hiratsuka, Toshiaki, 24742 Hirata, Yoko, 24808

Horiuchi, Masatsugu, 24539

Horwitz, Susan Band, 25054 Horska, Kvetoslava, 24591

Hosoya, Yukio, 24551 Hsieh, Jen-Chih, 24607 Hu, Guang, 25197 Huder, Jon B., 24564 Huganir, Richard L., 25152 Hughes, Diane L., 24863 Hwang, Yu-Wen, 24692

Imai, Enyu, 24976 Inagami, Tadashi, 24543 Inanobe, Atsushi, 24551 Inui, Hiroshi, 24543 Ischenko, Alexander, 25068 Isenman, David E., 25124 Ishikawa, Yoshinori. 25084

Jahangir, Arshad, 24551 Jeang, Kuan-Teh, 24940 Johnston, Stephen A,, 24699 Jolibois, Kern G., 24832 Joseph, Simpson, 24515

Kagawa, Yasuo, 24950 Kaguni, Laurie S., 24855 Kakizuka, Akira, 25253 Kambayashi, Yoshikazu,

Kang, Mona E., 25033

Katada, Toshiaki, 24551 Karlsson, Anna, 24591

Kato, Ryuichi, 24720

Kawashima, Seiichi, 25239 Kawahara, Yasuhiro, 24959

Kinane, T. Bernard, 24669 Kirkland, The0 N., 24818

24543

Kirsch, Thorsten, 25084 Kisselev, Oleg, 24519 Kiuchi, Kazutoshi, 24808 Klein, Michel H., 25124 Klett, Christoph, 25095 Knippels, Lion, 24813 Kobayashi, Kazuo, 24642 Kodadek, Thomas, 24699 Koepsell, Hermann, 25041 Koide, Masanobu, 24959 Kornberg, Arthur, 24665 Kuksis, Arnis, 24726 Kumagai, Naokazu, 24535 Kumar, M. Arun, 25176 Kunselman, Lori K., 24832 Kurachi, Yoshihisa, 24551 LaCorbiere, Monique, 25244 Lawrence, R. Michael, 24832 Lax, Sandra R., 24863 Leamon, Christopher P.,

Lee, J.-D., 24818 Lee, JiingDwan, 25009 Legendre, Laurent, 24559 Legoedec, Jocelyne, 25068 Lehner, Richard, 24726 Leturcq, Didier, 24818

Li, Jauyi, 24599 Levis, Mark J., 25001

Lightowlers, Robert N.,

Lindberg, Iris, 24910 Lissoos, Trevor W., 25132 Liu, Bin, 24967 Liu, Hong, 24933 Logan, Janette V. H., 24832 Lopez, Manuel Carlos, 24774 Lorimer, Heather E., 24647 Low, Malcolm J., 24967 LOW, Philip S., 24559, 24847 Lyons, Gary, 25244 Ma, Jianyi, 25108 Magnin, David R., 24832 Maller, James L., 25075 Malpeli, Giorgio, 24873

Marini, Joan C., 25162 Marekov. Lyuben N., 24916

Mastrocola, Teresa, 24779 Matsuda, Chie, 24950 Matsuno, Ryuichi, 25118 Mauger, Claire, 25068 McCormick, Kimberly A,,

McKechnie, Beth A,, 25108

Merrick, William C., 24863 Means, Anthony R., 25213

Miki, Miyako, 24642

Mileykovskaya, Eugenia I.. Milbrandt, Jeffrey, 24808

Miller, W. Todd, 25146 Miller-Hance, Wanda C.,

Missiaen, Ludwig, 25206 Miyata, Masaaki, 24720 Modrich, Paul, 24607 Montero, Mayte, 24751 Mookhtiar, Kasim A,, 24832

24847

24523

24683

24824

25244

Moriarty, Ann, 24818 Morii, Narito, 24535 Morino, Yoshimasa, 24758 Mosher, Deane F., 24838 Mossie, Kevin, 24880 Mukoyama, Masashi, 24539 Musacchio, Jose M., 24880 Myers, Robert W., 24785

Nagano, Yukio, 25118 Nagao, Shunsuke, 25239 Nagashima, Fujio, 24758 Nagata, Kiyoshi, 24720 Nakajima, Masatoshi, 24539 Nakayama, Ichiro, 24959 Narumiya, Shuh, 24535,

Nastuk, Mary A,, 25108 Neet, Kenneth E., 25176 Nelson, Hillary C. M., 25229 Nelson, Jeffrey W., 24599 Nemoto, Yasuo, 24535 Nicolai, Stefan, 24614 Nieves, Edward, 25054 Niles, Edward G., 24986 Nishino, Takeshi, 24642 Nobiling, Rainer, 25095 Nohara, Keiko, 24997

Nusing, Rolf M., 25253 Norton, Raymond S., 24707

O’Connor, Brigid M., 25054 O’Brien, Richard M., 24976

Ohta, Shigeo, 24950 Okamoto, Ken, 24642 Oomen, R., 25124 Orr, George A., 25054

Owen, Whyte G., 25176 Orrison, Bonnie M., 25162

Ozawa, Shogo, 24720 Ozawa, Kazuo, 25253

Pan, Qian-Wen, 24758 Paris, Sonia, 24531 Parmeggiani, Andrea, 24622,

Parry, David A. D., 24916

Pastan, Ira, 24847 Parys, Jan B., 25206

Pegg, Anthony E., 24572 Perez-Martin, Jose Manuel,

Perides, George, 25197 Peumans, Willy J., 25176

Poullet, Patrick, 24692 Poppe, Robert, 25041

Pratt, Richard E., 24539 Preiss, Thomas, 24523 hives , Carol, 24647 Puschel, Bernd, 25041

Rahaim, Peter T., 24792 Raibaud, Olivier, 24527 RajBhandary, Uttam L.,

Rao, A. Gururaj, 25176 Rasola, Andrea, 24779

Ravel, Joanne M., 24863 Raushel, Frank M., 24734

Raymackers, Jos, 24614 Rechler, Matthew M., 24892

25253

24692

24774

25221