FACULTY OF PHARMACYMONTHLY EXAMINATION

MICROBIOLOGY 2 (Virology)

I. MULTIPLE CHOICE: Choose the BEST ANSWER.

1. Host cell DNA within the viral capsid:A. virus B. viroid C. pseudovirion D. prion

2. Viral symmetry shaped like hallow protein cylinders which may be either rigid or flexibleA. icosahedral B. helical C. naked D. enveloped

3. Viral classification based on natural host range, mode of transmission, vector relationships and tissue tropismsA. Antigenic properties C. Biological propertiesB. Physiochemical properties D. Morphologic properties

4. Time in viral replication during which no virus is found inside the cellA. Latent period C. Incubation period B. Rise period D. Eclipse period

5. Enveloped viruses enter the host cell as cytoplasmic vacuole by this processA. endocytosis B. fusion C. adsorption D. direct penetration

6. Viral specimen should be stored at this temperature if not process for more than 4 days: A. 4ºC B. 25ºC C. -70ºC D. -20ºC

7. A cell infected with 2 different strains of segmented RNA viruses producing a genetically new virus. A. Genetic Drift C. Genetic ShiftB. Phenotypic Mixing D. Gene Reassortment

8. Coinfection of two normal viruses produced a composite virus with totally different capsid.A. Gene Reassortment C. Phenotypic MixingB. Phenotypic Masking D. Viral Complementation

9. These are giant-multinucleated cells formed from cell fusion as a result of virus infection A. Syncytia B. Plaque C. Inclusion bodies D. Hemagglutination

10. Indication for Serology Testing: A. Diagnosis of infection with culturable viral agentsB. Determination of nonimmune status in regard to rubella, measles, VZV, and HBVC. Monitoring of immunocompetent patientsD. Epidemiologic and prevalence studies

11. DNA virus causing kidney disease in immunocompromised individuals A. Papillomavirus B. Polyomavirus C. Mastadenovirus D. Erythrovirus

12. Genus of virus causing erythema infectiosumA. Roseolovirus B. Rhadinovirus C. Erythrovirus D. Varicellovirus

13. Kaposi’s sarcoma-associated herpesvirusA. HHV-5 B. HHV-6 C. HHV-7 D. HHV-8

14. Human papillomavirus type associated with high risk of malignancyA. HPV 1 and 4 B. HPV 6 and 11 C. HPV 16 and 18 D. HPV 42, 43 and 44

15. Adenoviruses type 40 and 41 causes this epidemics in children A. gastroenteritis C. keratoconjunctivitisB. interstitial pneumonitis D. pharyngoconjunctivitis

16. Poxvirus causing milker’s nodulesA. Orf B. cowpox C. pseudocowpox D. tanapox

17. Herpes simplex infection commonly seen in medical personnel handling infected specimen A. Herpes labialis B. eczema herpeticum C. herpes zoster D. herpetic whitlow

18. This is characterized by the appearance of clusters of vesicular lesions along a single sensory dermatomeA. Oral herpes B. herpes zoster C. herpes genitalis D. neonatal herpes

19. The only virus family assembled in the nucleus and whose envelop is from the host nuclear membrane A. Herpesviridae B. Poxviridae C. Adenoviridae D. Hepadnaviridae

20. Naked icosahedral ds DNA viruses with fibers projecting from the penton subunits of their capsid A. Mastadenovirus B. Simplexvirus C. Lymphocryptovirus D. Orthopoxvirus

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II. MATCHING: Match Column I with Column II

A. Viral Structure

21. Glycoprotein (D) A. genome

22. Peplomer (D) B. capsid

23. DNA or RNA (A) C. envelop

24. Protein coat (B) D. receptor

25. Lipid bilayer (C)

B. Viral Replication

26. Capsid disintegration (B) A. Adsorption

27. Packing of viral particle (C) B. Uncoating

28. Budding for enveloped virus (D) C. Assembly

29. Receptor binding (A) D. Release

30. Cell lysis for naked viruses (D)

C. Viral Genetics

31. Causes pandemics (C) A. Complementation

32. Causes epidemics (D) B. Transfection

33. Spreads antibiotic resistance (B) C. Genetic Shift

34. Occurs in segmented RNA virus (C) D. Genetic Drift

35. Minor changes in antigenicity (D)

D. Viral Isolation

36. Human neonatal lung (B) A. Primary cell culture

37. Minimal cell division (A) B. Diploid cell culture

38. Heteroploid (C) C. Continuous cell culture

39. Indefinite passage (C) D. Mixed cell culture

40. Engineered cell culture (D)

E. DNA Virus Diseases

41. Neonatal herpes (D) A. Roseolovirus

42. Fifth disease (B) B. Erythrovirus

43. Sixth disease (A) C. Lymphocryptovirus

44. Exanthem subitum (A) D. Simplexvirus

45. Infectious mononucleosis (C)

F. Latency Sites

46. Dorsal root ganglia (C) A. HSV 2

47. Sacral nerve ganglia (A) B. CMV

48. B lymphocytes (D) C. VZV

49. cranial nerve ganglia (C) D. EBV

50. Monocytes (B)

G. Cytopathic effect

51. Cowdry type A bodies (C) A. CMV

52. Downey type II cell (D) B. HPV

53. Koilocytic cells (B) C. HSV

54. “owl’s eye appearance” (A) D. EBV

55. plaques (C)

H. Cancer causing DNA viruses

56. Hepatocellular carcinoma (C) A. HPV

57. Nasopharyngeal carcinoma (B) B. EBV

58. Cervical intraepithelial neoplasia(A) C. HBV

59. Burkitt’s lymphoma(B) D. HHV

60. Kaposi sarcoma (D)

III. TRUE OR FALSE: Shade “A” for True and “C” for False.

61. Naked viruses are released thru lysis, thus they do not cause persistent productive infection. A62. All symmetrically icosahedral viruses are naked. C63. Viral glycoprotein which serves as receptors are only seen in enveloped viruses. C64. All viruses produce an mRNA which serves as protein template. A65. Most DNA viruses replicate in the cytoplasm except for Hepadnaviridae family. C66. During the latent phase of replication, no intracellular or extracellular virus may be detected. C67. Oncogenesis occurs when viruses inactivates tumor suppressor genes like p53 and RB. A68. Specimen for viral isolation should be collected from the affected site. A69. Heteroploid cell lines have an abnormal and variable number of chromosomes. A70. Serologic test are usually retrospective demonstrating seroconversion. A71. Poxviruses require a virion-associated transcriptase to make viral enzymes to synthesis all their nucleic acid. A72. CMV produces heterophile antibodies which react with animal red blood cells. C73. Smallpox lesions develop in a centripetal distribution and asynchronous manner. C74. Parvovirus B19 may cause aplastic crisis in patients with chronic anemia. A75. Varicella is a reactivation of zoster infection which has been dormant in the lymphoid tissues. C76. HPV viruses exhibit different tissue tropisms for epithelial cells. A77. HSV encephalitis is usually caused by HSV-2 in neonates and HSV-1 in older children and adults. A78. Stress, caffeine and sunlight may reactivate latent herpesviruses. A79. Polyomaviruses enter the body through the respiratory tract and goes to the kidney by viremia. A80. Betaherpesvirinae targets mononuclear cells and epithelial cells. A

”Nothing is more expensive than missed opportunities.”

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