Your test will be Thursday, June 7th and consists of 11 MC, 6 practicum questions and 2 BCRS. This study guide is due Tuesday, June 5th and will count as a grade.

CHROMATOGRAPHY:

1. Chromatography is a lab technique used to separate molecules

Paper chromatography2. Paper chromatography uses a mobile phase water or alcohol

and a stationary phase paper .

3. Paper chromatography is used to separate dyes/pigments. In paper

chromatography, inks separate according to their size and

__ solubility ___

4. In the diagram to the right:

a. Which ink color is most soluble? blue

b. Which ink color is least soluble? black

5. We used paper chromatography in the forensics unit to determine

which factory in Sligo Town processed the salad contaminated

with Salmonella .Gel Electrophoresis:

Name: _____________________

blue

pink

black

WORD BANKWater or alcohol solubility molecules size blue

black paper factory Salmonella

WORD BANKElectricity check further charge very little away

6. The process of gel electrophoresis is used to separates molecules by their size and charge

7. Electrophoresis is a process that uses electricity to pull molecules from one place to another.

8. Smaller molecules will move further from the wells.

9. Larger molecules will move very little away from the wells.

10. Negatively charged molecules will move towards the positive (red) electrode.

11. Positively charged molecules will move toward the negative (black) electrode,

12. We used gel electrophoresis chromatography in the forensics unit to

_check _ _ our results from the paper chromatography lab to determine which

_factory__ processed the salad contaminated with Salmonella.

13. Look at the chromatography results to the right. a. Which molecule/s (letters) were negatively charged? B, C, D, Eb. Which molecules (letters) were positively charged?__A

14. You should always _redo__ __the_____ __experiment__ make sure your results are correct.

WORD BANKElectricity check further charge very little away

wells

ED

CB

A

(+) electrode

(-) electrode

DNA Fingerprinting (Gel Electrophoresis)

1 2 3 4 5 6

15. In the DNA fingerprint result to the left, which two wells have

matching DNA samples? Wells 2_ and 4 _.

16. We used DNA fingerprinting in the forensics unit to determine which

_spinach__DNA sample matched the DNA sample from the contaminated

salad package to determine which farm was produced the contaminated salad green.

Urinalysis:WORD BANK

Acid base kidney disease neutral water diabetestoxins red

17. The kidneys function to filter out toxins and excess water to create the waste product known as urine.

18. Normal urine should test between 4.6 and 8.0 on the pH scale. Identify as acid/base/neutral:

8.0 base

7.0 neutral

4.6 acid

19 . Protein in the urine makes it turn cloudy and can indicate kidney disease___.

20. Glucose in the urine is tested using Benedicts solution and turns brick re d

color and can indicate what kind of health problems for the person? diabetes

Use the following table to answer question 20.

Campyloba S.aur V.

cter jejuni eus parahaemolyticus

SypmtomsNausea X X xVomiting X X x

Fever XDiarrhea X X x

Chills xCramps x x x

Bacteria in Stool x x

21. Which bacteria would cause nausea, vomiting, fever and diarrhea?Campylobactor jejuni

Microarray LAB TECHNIQUE

WORD BANKLook like genes Salmonella gene

Red blood cells carry oxygen and Neurons transmit messages carbon dioxide around the body. throughout the body.

22. Different types of cells (like red blood cells and neurons) look different and carry out different functions

because different _ genes __ are expressed or turned on in different cells.

23. The purpose of a positive control is to show what a positive results should _look like to use as a comparison.

24. We used the microarray lab technique in the forensics unit to determine which ingredient in the

contaminated salad was tested positive for the __Salmonella_.

BACTERIA STREAKING

red blood cells

neuron

+ -

control slide from microarray lab

WORD BANKAnimal Asexual pond runoff fence Exact copy

uni

25. Bacteria are uni - celled organisms that reproduce asexually

26. This type of reproduction results in bacteria that is an

exact copy of the bacteria it was produced from.

27. We used bacteria streaking in the forensics unit to determine which

_animal__ farm is responsible for contaminating the spinach farm with animal

waste containing salmonella transferred by __runoff____.

Use the following diagram to answer questions 28 and 29.

28. How does the animal waste from the pig farm effect the

lettuce farm? The runoff from the pig farm runs to the __pond__,

which is used to water the lettuce farm.

29 How does the fence affect the runoff from the cattle farm?

The cattle farm is surrounded by a __fence_ and remote

warning system that warns when animal waste is added to

runoff and may contaminate surrounding farms.

Performance Assessment

bacteria streaking

WORD BANKCloudy brick red probe changing

1)How would you test the pH of a liquid? You would test the pH of a

liquid using either pH paper or a SPARKS probe. With a SPARKS

_probe__ you would place the probe into the sample and wait for the

digital readout to stop __changing____.

2)Describe a urine sample that contains protein. A urine sample that

contains protein would have a _cloudy__appearance.

3)You have just completed an investigation to check for the presence of

glucose in a urine sample. A positive result would show a ____brick

red_ color.

4) Look at the picture below. Which PATTERN contains negatively charged molecules?

Stripped pattern

Red Electrode (+)

Black Electrode (-)

Use this graphic organizer to help you formulate your response to BCR prompt 2:

BCR #2: Salmonella Salami

Salami (also spelled “salame”) is a type of dried meat made of sausage, wheat, salt, cornstarch, and several spices, including black pepper. Recently, an outbreak of food poisoning caused by bacteria called Salmonella was traced to contaminated salami. After figuring out the brand of salami that was contaminated, how would you go about identifying the specific ingredient(s) that carried the bacteria?

Topic Sentence:

Evidence piece 1: The process used to identify the contaminated ingredient(s)

Evidence piece 2: How the process would identify the contaminated ingredient(s)

Evidence piece 3: The next steps to be done after identifying the contaminated ingredient(s)

Concluding Sentence: