V i co

V i co

ViCo.2STRUCTURED ILLUMINATION IMAGING SYSTEM MICROSCOPE

Personal computer(minimum requirements)

PC i3 or equivalentRam : 4GB minimumHDD : 500 GB

Software Operating system : Windows 32 or 64 bitViCo.2 software package (NIS Elements required)

System components (standard)Research fluorescence microscope NIKON ECLIPSE (NiE or TiE) with objectives, epi-illumination unit, filters, transmission source and fluorescence excitation source.Structured illumination module and control unit (V, W spatial modulator)Monochrome High-resolution CCD digital camera: NIKON DS-Qi2 or others monochromatic cameras drived by NIS

System features:Conventional (wide-field) image collection;Confocal (narrow-field) image collection;Higher light efficiency and lower photo-damage,

compared to laser scanning methods;Fully compatible with all available fluoro-chromes from

UV to IR;Spectral flexibility due to the use of conventional light

source instead of lasers;Three-dimensional resolution of 300 nanometer

DescriptionViCo.2 adopts a novel imaging method to obtain optical sections of microscopic specimens.Using a conventional fluorescence microscope, structured illumination via an V, W scanning mechanism and processing procedures, equivalent or better results are achieved compared to those of current, laser based confocal microscopes.Smooth transition between conventional, confocal imaging capabilities permits to optimize resolution versus speed and specimen photo-invasivity.Spectral and contrast flexibilities are guaranteed by automated multi-band and multi-mode acquisition capabilities. (Up to 6 channels - 2D, 3D, Z and T stack)

Controlunit

Structured illumination module

BIOMEDICAMANGONI s.n.c.via Garibaldi, 17956124 PISATel. +39 (050) 598133Fax +39 (050) 577740

Laboratoriovia G.Carducci, 1356010 Ghezzano (PISA)loc. la fontinatel. +39 (050) 879893web site: www.biomedicamangoni.it

DEALER :

VW

ConfocalConventional

The principle of ViCo.2

The imaging principle used with the Vico.2 is referred in the literature as "grid projection" or "structured illumination".A pattern mask of vertical line grids, is projected into the focal plane of the the objective and thru a spatial light modulator (v-w) controlled by piezo motors is shifted in defined steps. A synchronised monochrome CCD camera takes a picture in each grid position.The software will elaborate the acquired images to obtain the required image (confocal).

The result is a precise optical section through the specimen with no blurring, with elevate contrast and perfect for 3D reconstructions. Vico.2 characteristics are extremely closed to a modern confocal microscope and is suitable for q u a n t i t a t i v e a n a l y s i s o f fluorescence intensity.