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Vertebrate Anatomy Labs The Fetal Pig and You Slide 2 WEEK #1: Vertebrate Anatomy I: Skin and Digestive Systems Review descriptions and illustrations of tissues from lab manual Observe prepared slide of skin Fetal Pig Dissection *External anatomy *Follow dissection instructions sequentially as detailed in lab manual *Do not cut out a section of intestine (procedure 21.2 part J) Observe prepared slide of intestine cross-section Slide 3 External anatomy Slide 4 Male or Female? Slide 5 Opening the oral cavity 2. To expose structures in the mouth cavity, use heavy, sharp- pointed scissors to cut at the corners of the mouth along the line of the tongue. Continue to cut until the lower jaw can be lowered, being careful not to cut into the tissues in the roof of the mouth cavity. Continue to cut, pulling down on the lower jaw until your cuts reach the muscle and tissue at the back of the mouth. Cut through this muscle until lowering the jaw exposes the back of the mouth cavity. Slide 6 Slide 7 http://www.whitman.edu/biology/vpd/ Slide 8 Incisions to open the abdominal cavity. Incision 1 makes a shallow midventral incision from the base of the throat to the umbilical cord. Incision 2 cuts around the umbilical cord to the medial surface of each leg. Incision 3 cuts through the body wall laterally just posterior to the diaphragm. Incision 4 cuts laterally at the posterior margin of the abdominal cavity. Slide 9 Expose the digestive organs in the abdominal region by opening the posterior portion of the abdominal cavity. Begin the dissection by using the scalpel to make a shallow midventral incision from the base of the throat to the umbilical cord (incision 1). Cut lateral incisions (incision 2) around each side of the umbilical cord and continue the two incisions, one to the medial surface of each leg Now use the scissors to cut deep into one of the lateral incisions beside the cord until you penetrate into the abdominal cavity, piercing the parietal peritoneum. Use the scissors to cut through the body wall along the two lateral incisions to the legs and around the umbilical cord. Pull lightly on the umbilical cord. If your dissection is correct, you will see that the umbilical cord and ventral wedge of body wall could be reflected, or pulled back, toward the tail, except for a blood vessel, the umbilical vein. This vein passes from the umbilical cord anteriorly toward a large brown organ, the liver. Cut through this vein, leaving a stub at each end. Tie a small piece of string around each stub so you can find them later. This should free the flap of body wall, which may now be reflected toward the tail, exposing the abdominal organs. Slide 10 Slide 11 Slide 12 Slide 13 Slide 14 Slide 15 Pancreas spleen Stomach Large intestine Small intestine Liver Slide 16 Cecum Slide 17 WEEK #2: Vertebrate Anatomy II: Circulatory and Respiratory Systems Fetal Pig Dissection *Follow dissection instructions sequentially as detailed in lab manual *Dissection of neck and thoracic cavity *Identify heart structures and great vessels *Identify structures of the systemic circuit in the thorax *Identify structures of the systemic circuit in the abdominal cavity *Examine fetal circulation *Identify structures of the respiratory system Examine Sheep Heart demo Slide 18 Week Two The Circulatory and Respiratory Systems Begin the dissection by opening the thoracic cavity, which houses the heart and lungs, and making an incision that extends to the jaw. Use scissors to deepen the superficial incision previously made anterior to the abdominal cavity, and continue deepening this incision to the base of the lower jaw. Cut through the body wall in the region of the thorax, clipping through the ribs slightly to the right or left of the sternum (the flat bone lying midventrally to which ribs attach). Continue the incision past the rib cage to the base of the lower jaw. Using the blunt probe to separate tissues, carefully remove the skin and muscles in the neck region. You will expose the thymus gland on each side of the neck. Slide 19 Neck region and Thoracic Cavity Slide 20 Expose the heart lying in the pericardial cavity between the two pleural cavities. Gently push open the rib cage, using scissors and a probe to cut through muscle and connective tissue. Another lobe of the thymus gland will be seen lying over the pericardial sac housing the heart. The wall of the pericardial sac is a tough membrane composed of two fused coelomic epithelial linings, the parietal pericardium and the parietal pleura. Cut into and push aside the pericardial sac. Carefully dissect away membranes adhering to the heart until you can identify the four chambers of the heart Slide 21 Neck region and Thoracic Cavity Slide 22 Thyroid gland Thymus Left ventricle Right ventricle Larynx Coronary artery and vein Slide 23 Veins near the Heart Slide 24 Slide 25 Slide 26 Arteries near the Heart Slide 27 Slide 28 Slide 29 Abdominal branches of Aorta and Vena Cava Slide 30 Slide 31 Respiratory system Slide 32 WEEK #3: Vertebrate Anatomy III: Excretory, Reproductive and Nervous Systems Fetal Pig Dissection *Identify structures of the excretory system-kidney *Identify structures of the male reproductive system *Identify structures of the female reproductive system Examine Pregnant Pig Uterus demonstration materials Observe prepared slide of kidney Observe prepared slide of neuron Observe prepared slide of spinal cord cross-section Cow eye dissection Slide 33 Week Three Excretory, Reproductive, and Nervous systems Male Slide 34 Week Three Excretory, Reproductive, and Nervous systems Female Slide 35 Renal System Slide 36 Urinary System Urinary bladder Slide 37 Female Reproductive Tract Ovary Slide 38 Male Reproductive Tract Penis Epididymis Testes Slide 39 Penis Vas Deferens Testicle Epididymus Urinary bladder Umbilical artery Rectum/colon Slide 40 Vas Deferens Ureter Slide 41 Slide 42 Uterus with aminiotic sac visible Slide 43 Cow Eye Anatomy Follow dissection instructions in the lab handout Slide 44 Cow Eye Anatomy Slide 45 Microscope Slide Review Slide 46 Ileum cross section Slide 47 Integument Note the Stratified Squamous Epithelium of the skin Slide 48 Neuron Slide 49 Spinal Cord cross-section Slide 50 Kidney Renal Cortex Renal Medulla