UNIVERSITY OF SANTO TOMAS

FACULTY OF PHARMACY

DEPARTMENT OF MEDICAL TECHNOLOGY

Principles and Strategies of Teaching in Health EducationTEACHING DEMONSTRATION

ACTIVATED PARTIAL THROMBOPLASTIN TIMEI. OVERVIEW

Venipuncture is the process of obtaining intravenous access for the purpose of intravenous therapyor forblood samplingofvenousblood.II. PRINCIPLE

Venipuncture is an invasive procedure, which carries a small risk to the patient as well as to the phlebotomist. The following techniques and procedures are used to minimize these risks. In addition, procedures for specimen handling are followed in order to preserve specimen integrity. Venipunctureis the collection of blood from a vein, usually for laboratory testing. Most of the time, blood is drawn from a vein located the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic).III. MATERIALSA. Blood collecting trays.

B. GlovesC. Sterile Needles

D. Push Button Winged butterflies

E. Evacuated tubes

F. Tourniquet

G. Antiseptic

H. Clean Gauze

I. AntisepticIV. METHODA. Identify the patient. Outpatients are called into the phlebotomy area and asked their name and date of birth. This information must match the requisition.B. Wash hands and put on gloves.C. Position the patient with the arm extended to form a straight-line form shoulder to wrist.D. Select the appropriate vein for venipuncture.E. Apply the tourniquet 3-4 inches above the collection site.F. Clean the puncture site by making a smooth circular pass over the site with the 70% alcohol pad.G. Pull the skin tight with your thumb or index finger just below the puncture site.

H. Holding the needle in line with the vein, use a quick, small thrust to penetrate the skin and enter the vein in one smooth motion.I. Holding the hub securely, insert the first vacutainer tube following proper order of draw into the large end of the hub penetrating the stopper. Blood should flow into the evacuated tube. J. After blood starts to flow, release the tourniquet and ask the patient to open his or her hand.K. When blood flow stops, remove the tube by holding the hub securely and pulling the tube off the needle. V. INTERPRETATION:

If a blood sample is not attainable:

A. Reposition the needle to the median cubital.

B. Ensure that the collection tube is completely pushed onto the back of the needle in the hub.

C. Use another tube as vacuum may have been lost.

D. Loosen the tourniquet.

E. Probing is not recommended. In most cases, another puncture in a site below the first site is advised.

F. A patient should never be stuck more than twice unsuccessfully by a phlebotomist.The Supervisor should be called to assess the patient.