Spectrophotometric Simultaneous Determination of Caffeine ...
Validate spectrophotometric measurements with certified UV/Vis reference materials
Transcript of Validate spectrophotometric measurements with certified UV/Vis reference materials
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Reliable measurements – assured!
Validate spectrophotometric measurements and document their traceability with certified UV/Vis reference materials
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• ISO 9001/17025 requires measurement devices to be checked on a regular basis
• GLP (good laboratory practice) governs the responsibility to provide high-quality, accurate data, including instructions for testing devices and reference materials
• Ph. Eur. “European Pharmacopoeia” and USP 857 requires any UV/Vis spectrophotometers in use to be qualified on a regular basis
• USP „U.S. Pharmacopeial Convention“ governs the verification of UV/Vis spectrophotometers
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Using certified reference materials to check the measurement device guarantees:
High-quality measurement results
Traceability to international standards
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UV/Vis electromagnetic spectrum:
The Hellma Analytics calibration laboratory is accredited for manufacturing and certifying UV/Vis reference materials from 190 to 890 nm
x-ray ultraviolet radiation visible light infrared
Electromagnetic radiation spectrum:
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The following UV/Vis spectrophotometer parameters should be checked on a regular basis:
Photometric accuracy (absorbance)
Wavelength accuracy
Stray light level
Spectral resolution
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Checking the photometric accuracy (absorbance)
… or with a Neutral Density Glass Filter
Wavelength in nm
I0 I
Light source Detector
using a Potassium Dichromate Liquid Filter
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Checking the wavelength accuracy
… or with a Holmium Glass Filter
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Light source Detector
using a Holmium Liquid Filter
Wavelengths in nm
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Checking wavelength and photometric accuracy using a Didymium Glass Filter
Wavelength in nm
Photometric accuracy :
Wavelength accuracy:
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Wavelength in nm
I0 I
Light source Detector
Checking the stray light:
with Potassium Chloride (UV1), Sodium Iodide (UV10), Sodium Nitrite (UV11)
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Checking spectral resolution using toluene in n-hexane
Wavelength in nm
I0 I
Light source Detector
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Traceable
Reliable measurements through the use of certified reference materials.
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SRMs
All reference materials certified by Hellma Analytics are traceable to NIST standards
Hellma calibrates its spectrophotometers with standard NIST reference materials on a daily basis
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The Hellma Analytics calibration laboratory is:
...certified in accordance with ISO 17025
…Germany’s only accredited DAkkS calibration laboratory for UV/Vis reference materials
DAkkS - Calibration Certificate from Hellma Analytics
Reliability for Certified Reference Materials
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In the DAkkS calibration certificate the measured values for each filter are documented.
DAkkS - Calibration Certificate from Hellma Analytics
Reliability for Certified Reference Materials
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Glass filter sets for verification of the wavelength accuracy and/or photometric accuracy Quick and easy solutions for calibrating spectrophotometers
Type: 666-S000 Type: 666-S001 Type: 666-S002
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Liquid filter sets Quick and easy solutions for calibrating spectrophotometers
Type: 667-UV100
Set for checking stray light in accordance with Ph. Eur.
Type: 667-UV200
Set for checking resolution in accordance with Ph. Eur.
Typ: 667-UV003
Complete set for performing checks in compliance with Ph. Eur.
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Reference plate with holmium oxide strips for checking the wavelength accuracy and photometric accuracy of microplate readers The dimensions correspond to a standard microtest plate. Type: 666-R113
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Reliable Measurements – Assured!
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For further information, please visit: www.hellma-analytics.com/standards or contact us: [email protected]