VACCINES

Akash Mali,India

Basic laboratory services and media preparation for vaccine production

•Water

• Electricity

•Capital equipment

The production of a bacterial vaccine

•The Organism To Be Produced(Brucella abortus Strain 19)

•Growth Medium

•Fermentation Equipment

•Temperature And Ph Control(± 0.5°C.and 121°C down to 37°C in 20 to 30)

Lyophilization

•Minimum Space ,Optimized Fermentor Design, Range -5 L-25000 L •Agitator(Top/bottom) ,Double mechanical Seal

SELECTING THE STRAIN FOR VACCINE PRODUCTION

GROWING THE MICRO-ORGANISM

ISOLATION & PURIFICATION OF MICROORGANISM

INACTIVATION OF ORGANISM

FORMULATION OF VACCINE

Upstream

downstream

5

Alum

Production strain Seed culture Fermentation Purification

Adjuvant

FillingLabeling Packaging

Inoculation

Inspection

Ultrafilteration

Glycine soln

Formaldehyde soln

Monosodium glutamate

Inactivation

Thimersol

Stabilizer

Preservative

Dilutant

Clostridium.tetani.(anaerobic species of pathogenic bacteria)