V National Conference BIFI, Zaragoza, Febrero 2011

V National Conference BIFI, Zaragoza, Febrero 2011

OLGA [email protected]

OVERVIEW OF EXPERIMENTAL FACILITIES AT BIFI-BIO

http://bifi.es/index.php


Molecular Biology: Genetic engineering and recombinant proteins (cloning, mutagenesis, recombinant proteins expression with biotechnology or biomedical interest).

Biochemistry: Proteins and nucleic acids purification using Fast Performance Liquid Chromatography, ionic exchange, affinity or molecular exclusion columns. Small protein or chemical compounds purification.

Biophysics of Biological Molecules: Characterization of protein stability, protein-protein and protein ligand interaction, and protein function.

Cell Biology: Prokaryotic and eukaryotic cell culture for the expression of recombinant proteins, determination of the efficacy and toxicity of bioactive compounds and apoptosis studies. Stem cell cultures for cellular differentiation and dead cell studies.

High Throughput Screening (HTS): Screening methods for experimental identification of bioactive compounds against pharmacologic targets.

Crystallography: Obtaining crystallographic models of proteins: preparation of crystals, analysis by X ray diffraction, data processing and molecular structure resolution.

GENERAL AREAS OR ACTIVITIES PERFORM AT BIFI FACILITIES


LABORATORY USERS AT BIFI


RESEARCH GROUPS USERS OF BIFI FACILITIES


NATIONAL COLLABORATORS

CIPF VALENCIAMª Eugenia Armengod

UAB BARCELONASalvador VenturaMireia García-Viloca

Univ. País VascoArturo MugaMª Ángeles UrbanejaOscar Millet

CSIC Rocasolano MADRIDJulia Sanz-AparicioJuan Hermoso Mª Ángeles Jiménez

UMH Elche ALICANTEJose Luis Neira

C.S.I.C. C.I.B. MADRIDÁngel MartínezAitor Hernández-Ortega

Barcelona Supercomputing Centre BARCELONA

Víctor Guallar

Instituto de Bioquímica Vegetal y Fotosíntesis, SEVILLA

Miguel A. de la RosaAntonio Díaz Quintana

UNIZAR ZARAGOZAJesús MartínezMiguel Pocoví

INIBICA CORUÑA

Jose Francisco Pérez Llerena

Universidad Pablo Olavide SEVILLA

Laura Ledesma

Hospital ClínicoZARAGOZA

Ángel LanasTrinidad Serrano

UAM MADRIDMiguel Fernández Moreno Rafael Garesse AlarcónJuan J. Arredondo Lamas

I3A ZARAGOZALuis José Fernández

INA ZARAGOZAJesús Martínez De La FuenteValeria Grazú

CBM CSIC MADRIDCelia Perales

INSTITUTO DE CATÁLISIS CSIC MADRID

Jose Miguel PalomoJose Manuel Guisan


NATIONAL UNIVERSITY OF SINGAPORE (Singapur)Jayaramam Sivaraman

U. COIMBRA (PORTUGAL)Maria Joao Moreno

Rui M. Brito

U. COPENHAGEN (DINAMARCA)

Marina Kasimova

JHU BALTIMORE (EEUU)Ernesto Freire

UNAM MEXICO(MEXICO)

Enrique García-HernándezAlfredo Lagunas

U. COLONIA (ALEMANIA)Agapios Sachinidis

Emory University, Atlanta (EEUU)

Dale Edmondson

U. VIRGINIA(EEUU)

Paul S. Hoffman

Stem Cell Center, New York (EEUU)

Michael Xavier Doss Jesudoss

INTERNATIONAL COLLABORATORS


We moved to the new I+D building in February- Marzo 2010.

Laboratories were distributed according to techniques and equipment.

This allows the researches an easier use of the facilities and an efficient space distribution.

New incorporation of researchers and equipments is easier.

Biophysics, Biochemistry and Molecular and Cellular Biology sections at BIFI are distributed in laboratories dedicated to specific subjects.

They are distributed in block number 2 and floor 0 and block number 1 and floor 2 of the I+D building.

I+D BUILDING IN CAMPUS ACTUR OF ZARAGOZA UNIVERSITY ORGANIZATION


Block 2 Floor 0


Block 1 Floor 2


12 individual working places composed by a desk and laboratory bench. Predoctoral students are working in this laboratory.

Laboratorio general I

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de espectroscopia

Fluorescence spectrophotometer:

Cary Eclipse (Varian)Multi-sampler Peltier

module

UV/visible Spectrophotometer Cary 100

BIO (Varian)Multi-sample Peltier module

Espectrofotometer NanoVue (GE Healthcare)

Micro-volume samples

Light scattering Dynapro Nanostar (Wyatt)

Hydrodinamic radius determination and molecular

protein mass.

Spectropolarimeter and fluorescence spectrophotometer: Chirascan from Applied Photophysics. Structural characterization of proteins in steady state and kinetic state (stopped-Flod module)

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de calorimetría y SPR

Differential Scanning Calorimeter VP-DSC

(MicroCal, GE Healthcare)Study of energetic structural stability

of proteins

2 units of Isothermal Titration Calorimeters VP-ITC

(MicroCal, GE Healthcare)Study of energetic biomolecule

interaction

Surface Plasmon Resonance Equipment Biacore T200 (GE

Healthcare)Study of biomolecules interaction in

kinetic and steady-state

Isothermal Titration Calorimeter AUTO-ITC200(MicroCal, GE Healthcare)

Study of energetic biomolecule interaction. Multi-sample and

automatic

V National Conference BIFI, Zaragoza, Febrero 2011Cámara fría 4ºC y cámara -20ºC

In the temperature controlled room (2.0.04) the storage of the samples at 4ºC or -20ºC is possible. The size of the room allows also to perform experiments such as protein purification or separation and analysis, using electrophoresis and chromatographic techniques.

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Rayos X

Automatic systems of liquid dispensation of liquids for macromolecules crystallization and management system and well visualization.A/ PERKIN ELMER JANUS MINI.B/ INNOVADYNE NANODROP II. C/ FORMULATRIX ROCK IMAGER 182.D/ LEICA Stereoscopies.

X-Ray DiffractometerAnalysis of crystal proteins

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de cromatografía y microscopía

The Leica DMI6000 B inverted microscope for biomedical research offers Differential Interference Contrast (DIC) for relief imaging of specimens with varying indices of refraction. The intuitive, automated Leica DMI6000 B is ideal for fluorescence, live cell, time-lapse imaging; high-speed multi-fluorescence optical sectioning; and micro-manipulation.

2 Fast protein liquid chromatography equipments (FPLC)

Technique used for the separation or purification of proteins and other polymers from complex mixtures.

High-performance liquid chromatography equipment (HPLC)

Technique used for identifying, quantifying and purifying the individual components of the mixture.

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio general II

6 individual working laboratory bench. Senior researchers and technicians are working in this laboratory.

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio Biología Molecular

Laboratory with contains the general equipment for performing molecular biology experiments:

2 PCR thermal cyclers. DNA and protein electrophoresis system. Protein immunoblot Inmunoblot system. 2D Electrophoresis system Electroporator used for the transformation of bacteria, yeast. Purification water system Elix 3 (Millipore) and Ultrapure purification system MilliQ (Millipore). Ice maker.Manipulation yeast cabin.Safety chemistry cabin.

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio Cristalografía

This is a thermostatic chamber at 18ºC temperature with an anaerobic system and a cold room at 4ºC.

V National Conference BIFI, Zaragoza, Febrero 2011Sala de Congeladores y revelado

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Cultivos Celulares I

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Centrifugación

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Cribado

This laboratory contains: Synergy HT Multi-Mode Microplate Reader A filter-based high temperature fluorescence microplate reader FluoDia T70 Multi-plate well centrifuge

V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Cultivo Celular


THANK YOU FOR YOUR ATTENTION!!

V National Conference BIFI, Zaragoza, Febrero 2011

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