_uv/vis reflection spectrometer nanofilm_refspec

_uv/vis reflection spectrometer nanofilm_refspec

Abstract With the nanofilm_refspec

the reflection of light R

(range: UV…VIS) from an

air-water interface can be

detected. This spectrum

covers the applications

from protein to dye-

containing monolayers.

Besides the spectral de-

tection of the reflection

(∆R over wavelength) a

time-based measurement (∆R

over time) is also easily

performed.

The nanofilm_refspec is

completely PC controlled.

The software makes meas-

urements easy, controlled

via function tree and Di-

rect-Control-Button. The

script language allows the

automation of complex

measurements and specific

organization of the func-

tion tree.

The software interface to

Langmuir troughs allows

measurement versus trough

parameters ( ∆R over sur-

face pressure, area,

etc.). The adaptation to

troughs from Accurion and

KSVNIMA is included, adap-

tion to other troughs

available on request.

Benefits Optimized for the

liquid/gas interface Internal reference

with shutter adaption to Langmuir

troughs

In detail...

Trough Electronic

Shutter Lamp

Fiber

Water

USB-cable

connection to trough (serial)

PC

Spectro- meter

Lightsource

Shutter

Schematic illustration of the nanofilm_refspec

Technical specification:

light source: tungsten & deuterium lamp

spectral range: 240 nm … 1000 nm

max. resolution ∆R:± 0.002 %

spectral measurement ∆R(λ)

kinetic measurement ∆R(λ, time)

trough measurement ∆R(λ, trough parameter)

Communication parameter e.g.: with LB-troughs: surface pressure (KSVNIMA) area/molecule

Setup of a nanofilm_refspec adapted to a Nima trough (not in-luded in the basic setup)

Adaption of trough control is included (KSVNIMA as standard, other troughs on request) completely PC-controlled

dye monolayer (S8:C20 = 1:9) (S8:C53H85N2O6Cl) S8 = Distearyl Oxacarbocyanine, C20 = Arachidic acid with the surface pressures: p = 0 to p = 65 mN/m.

Reflection spectra of the dye monolayer above at different surface pressures.ressures

Typical samples

dye monolayers

fatty acids

proteins interacting with charged/uncharged

lipids

LB-films on solid structures

liposomes interacting with lipid films

Examples of application in literature

Giner-Casares JJ, de Miguel G, Perez-Morales M,

Martin-Romero MT, Camacho L, Munoz E.(2009)

Effect of the Molecular Methylene Blue Aggregation

on the Mesoscopic Domain Morphology in Mixed Mo-

nolayers with Dimyristoyl/Phosphatidic Acid.

J. Phys. Chem. C, 113: 5711-5720.

Sandez-Macho I, Gonzalez-Lopez J, Suarez-Varela A,

Mobius D (2005)

Influence of the Environment on Photoinduced

Electron Transfer: Comparison between Organized

Monolayers at the Air-Water Interface and Monolay-

er Assemblies on Glass.

J. Phys. Chem. B 109: 22386-2239

Software: function tree and measurement control window

Europe: Accurion GmbH Stresemannstrasse 30 37079 Goettingen, Germany Phone: +49[0] 551.999 60.0 Fax: +49[0] 551.999 60.10 E-Mail: info@accurion.com Web: www.accurion.com USA: Accurion Inc. 514 Progress Drive, Suite G Linthicum Heights, MD 21090 Phone: +1[0] 410.636.3355 E-mail: info@accurion.com Web: www.accurion.com India: Accurion Scientific Instruments Pvt. Ltd. Mr. Narayana Sharma Flat 307, S.S Residency 29th Main, 2nd C Cross BTM Layout, 1 Stage, 1 Phase Bangalore 560 068, India Mobile: +91[0] 98450.04273 Phone: +91[0] 80.2668.9178 E-mail: sharma@accurion.com Web: www.accurion.com