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Transcript of US $ Liquid Chromatography - 13-66 New.pdf  Liquid Chromatography Order: 1.800.325.3010...

  • Liquid

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    13HPLC: Small Molecules

    Column Selection

    HPLC Column Selection: Small Molecules (7.5) ....................................................................................................... SUPELCOGEL TPR-100, ODP-50chelating compounds ......................................................... Discovery C18, C8, RP-AmideC16; SUPELCOGEL TPR-100, ODP-50drugs, basic ...................................................................... Discovery C18, C8, RP-AmideC16; Discovery Cyano, Discovery HS F5

    reversed drugs in serum ................................................................................... Hisep; Discovery C18, RP-AmideC16, C8; Discovery HS F5phase explosives ..................................................................................................... Discovery C18, C8, RP-AmideC16; Discovery Cyano

    nucleosides ................................................................................................... SUPELCOSIL LC-18-S; Discovery C18, RP-AmideC16nucleotides ................................................................................................... SUPELCOSIL LC-18-T; Discovery C18, RP-AmideC16peptides ................................................................................................................................... Discovery C18, C8, RP-AmideC16

    nonionic sugars ............................................................................................................................................................ SUPELCOSIL LC-NH2taxol, taxanes ..................................................................................................................................................... SUPELCOSIL LC-Ftricyclic antidepressants ............................................................................. SUPELCOSIL LC-PCN; Discovery C8; Discovery Cyanovitamins, water soluble ............................................................................................................. Discovery C18, C8, RP-AmideC16

    water hydrophobicsoluble interaction peptides ........................................................................................................................................................................... TSK-GEL

    size oligosaccharides ........................................................................................................ SUPELCOGEL Ag1, Ag2; TSK-GEL Oligo-PWexclusion polymers/oligomers, hydrophilic .............................................................................................................................. TSK-GEL PWXL

    ion anions, organic (- charge) ................................................................................................................................. SUPELCOSIL SAX1exchange cations, organic (+ charge) ........................................................................................................................... SUPELCOSIL LC-SCX

    ionic ion pairing acids, organic ............................................................................................................................ Discovery C18, Discovery HS C18amines, organic ......................................................................................................................... Discovery C18, Discovery HS C18

    ion acids, organic ........................................................................................................................................... SUPELCOGEL H, C610Hexclusion carbohydrates, sugars ................................................................................................... SUPELCOGEL C611, K, Pb, Ag1, Ag2, Ca

    GENERAL ................................................................................................. Discovery Cyano, LC-NH2-NP, LC-Si, Discovery HS PEGnormal aflatoxins ........................................................................................................................................................... SUPELCOSIL LC-Siphase steroids .......................................................................................................................................................... SUPELCOSIL LC-Diol

    vitamins, fat soluble ................................................................................................................................ SUPELCOSIL LC-NH2-NP

    organic soluble fatty acids ......................................................................................................................................................... SUPELCOSIL LC-18reversed polyaromatic hydrocarbons ........................................................................................................................... SUPELCOSIL LC-PAHphase polymers, hydrophobic .................................................................................................................................... SUPELCOSIL LC-18

    triglycerides ...................................................................................................................................................... SUPELCOSIL LC-18vitamins, fat soluble ................................................................................... Discovery C18, C8, RP-AmideC16, SUPELCOSIL LC-8

    size exclusion polymers/oligomers, hydrophilic ................................................................................................................................ TSK-GEL HHR

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    14HPLC: Small MoleculesDiscovery Reversed-Phases

    Characteristics of Discovery Reversed-PhasesCoverage Pore Diameter Surface Area

    Phase mole/m2 %C Endcapped Angstroms m2/gHS C18 3.8 20% yes 120 300C18 3.0 12% yes 180 200RP-AmideC16 2.6 11% yes 180 200C8 3.4 7.5% yes 180 200HS F5 4.0 12% yes 120 300Cyano 3.5 4.5% yes 180 200HS PEG 3.8 12% no 120 300

    Valuable, Different Separations versus C18While C18s from different sources can provide significant differences in re-

    tention and selectivity, these differences are frequently small and not sufficientto produce really valuable, improved separations. The Discovery suite of re-versed-phases is designed to be complimentary to C18 by combining polarfunctionality with the standard alkyl/hydrophobic phase. The result Its muchmore likely to achieve an improved, valuable separation with a polarfunctionalized reversed-phase than with another C18.

    Ideal for all small molecule HPLC applicationsDesigned to meet the exacting requirements of pharmaceutical analysis and

    purification, Discovery columns are also ideal for all other application segmentsrequiring reversed-phase HPLC, including:

    - food and beverage - agriculture

    - environmental - consumer products

    - clinical - industrial chemical

    - petrochemical - and more .

    C18

    RP-AmideC16

    HS F5

    Cyano

    HS PEG

    C8

    O Si

    CH3

    (CH2)17 CH3

    CH3

    Comprehensive Suite of Alkyl and Functionalized Phases

    = O On

    O Si (CH2)3NHCO-(CH2)14 CH3

    CH3

    CH3

    O Si

    CH3

    (CH2)17 CH3

    CH3

    O Si

    CH3

    (CH2)3 CN

    CH3

    O Si

    CH3

    (CH2)7

    CH3

    CH3

    O Si

    CH3

    CH3

    (CH2)3

    F F

    F

    FF

    HS C18

    On the next page,begin to rediscover

    method development...

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    15HPLC: Small Molecules

    Rediscover Method Development

    0 2 4 6 8 10 12Min

    1

    2

    3

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    0 2 4 6 8 10Min

    1

    2

    4

    3

    Take Advantage of the Discovery Suite of Reversed-Phases

    Whether youre developing a separation thatmust be perfect, or youre just not satisfied withyour reversed-phase separation...

    Turn to Discovery!

    a suite of HPLC columns featuring function-alized reversed-phases designed to provide dif-ferentiated separations vs. C18 based on uniquecombinations of polar and hydrophobic retention.

    The Discovery suite of reversed-phases en-ables you to optimize your separation..., e.g.

    - retention - selectivity

    - resolution - analysis time

    ...while minimizing method development time.

    Hydrophobicity:Discovery Reversed-Phases

    Discovery RP-AmideC16 Delivers Better Peak Spacing and Faster Analysis!

    Scientists have long known polar embedded phases such as Discovery RP-AmideC16 provide unique retention and selectivity compared to C18s. Taking ad-vantage of Discovery RP-AmideC16s unique retention and selectivity results in abetter separation vs. C18.

    - Unique selectivity - peak reversal of compounds 3 and 4

    - Better peak spacing - less dead space between peaks 1 and 2

    - Higher assay throughput - 20% reduction in analysis time

    - Improved resolution of critical pea