Biomarker Immunoassays: Transforming the Process of Discovery
URIMEM, will facilitate biomarker discovery and change the face of medicine
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Transcript of URIMEM, will facilitate biomarker discovery and change the face of medicine
URIMEM, will facilitate biomarker discovery and change
the face of medicine
高友鹤YOUHE GAO
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
Advantages of Urine Proteome
• Non-invasive• Simpler than plasma• Reflects changes of plasma• Close to kidney and prostate
Urine Proteome Studies
before 2000
2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 20130
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PUBMED
PUBMED
Disadvantages
• Big variation• Need more cases to validate
Urine: may even better than blood for biomarker study
• Urine– Suppose to be waste– Changes were
maintained if not amplified
• Blood– Suppose to be
homeostatic– Changes were minimized
Problems
• More sample• Higher throughput MS• More analyzing power
Problems to save samples
• Very diluted• Protein degradation
Possible ways to save
• Precipitate: organic solvent consuming • Freeze dry: energy and labor consuming• Extraction: cost• Not suitable for large numbers of samples
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Urimem
UrimemUrinary proteins
dried on a filter and stored in vacuum
Why is that important?
Changed the face of medicine
• “Hard as it is to believe today, a single concept developed by Dr. Henry Plummer (1874 – 1936) at the beginning of the 20th century changed the face of medicine.”
http://www.mayoclinic.org/tradition-heritage/medical-records.html
Changed the face of medicine
• The concept was a centralized medical record, stored in a single repository, and capable of traveling with the patient. (1907)
• Now– paperless – filmless – chartless
http://www.mayoclinic.org/tradition-heritage/medical-records.html
Biobanking: foundation of personalized medicine
• Blood• Urine• Skin cells• Organ• Tissue• other things taken from a body
Hewitt RE. Curr Opin Oncol. 2011 Jan;23(1):112-9. doi: 10.1097/CCO.0b013e32834161b8.
Do we have enough? Why not?
• Invasiveness• Storage expense• Limited biological information (hair, nail)
URIMEM is the answer
• Invasiveness: You can’t hold your urine for a few hours.
• Storage expense: Proteins dried on a membrane can be stored in vacuum even at room temperature for quite a while.
• Limited biological information: much more complicated information from the whole body especially from the secretory system
Can we decode urine proteome?
• Not yet• But having large number of samples will help
Acknowledgement• Lulu Jia• Xuejiao Liu• Liu Liu
• Youhe Gao 高友鹤 • [email protected]• 13910861559
• Department of Nephrology, PUMC Hospital: Mingxi Li
• Funding from 973 Project