Unit+4+cytosskeleton+part+2+slides

7/23/2019 Unit+4+cytosskeleton+part+2+slides

1/82

!"#$ &'()*+,-,()$. /01( 2


2/82

!$ )3,134,5 )6 (7, 68$"#)$0- "-0**,* )6 0"#$ 94$:4$;


3/82

&-0**,* )6 0"#$ 94$:4$; 9-'?:4*0**,>9-'=

@= A)$)>,1 94$:4$;?*,B8,*(10#)$


4/82

&-0**,* )6 0"#$ 94$:4$; 9-'?:4*0**,>9-'=

2= )(, $8"-,)#:, ,D"70$;, )$ EF0"#$G :41,"(*


5/82

&0


6/82

&-0**,* )6 0"#$ 94$:4$; 9-'?:4*0**,>9-'=

K= /-8*?9019,: ,$: &0


7/82

&-0**,* )6 0"#$ 94$:4$; 9-'?:4*0**,>9-'.

M= N)1>4$*. /-8* ,$: 94$:4$;


8/82

N)1>4$* )(,>)$)>,1 0::4#)$

0( (7, ;1)54$;


9/82

>4$8* ,$:

&-0**,* )6 0"#$ 94$:4$; 9-'?:4*0**,>9-'=

R= A4$8* ,$: "0


10/82

R9= !S/ 2LK ")>4$8*F

,$: "0


11/82

Nucleation of branched actin filaments by Arp2/3 complex

Fig. 33-13!"#$%&'() +,,- .$ /01234%5678549&4%: ;88 %&'()5 %454%943:


12/82

U= !"#$ C-0>,$( *,3,14$; ,$(* (7,$

*,3,1L ;,$,10--' -,034$; (7, $,5-' 6)1>,:


13/82

V= &)C-4$. T4$:* () !H/F

")$(04$4$; *898$4(* 0$:

)(,* C-0>,$(

*,3,14$;? :4*0**,>9-' 0(

(7,W)-:W !H/ *898$4(

")$(04$4$; ,$:= P61)>/)--01: X01$*705 (,D(Q


14/82

Y= N4-0>,$( *(094-4Z4$;


15/82


16/82

^0>,--01L 0"#$ C--,: ,D(,$*4)$* 0( -,0:4$; ,:;, )6 "105-4$;

",--= XD(,$*4)$ 4* :143,$ 9' 0"#$ C-0>,$(


17/82

A model for lamellar extension by actin filament assembly

at the leading edge & disassembly at the base/rear of the

lamella.

EM!"#$%&'() +,,- .$ /01234%5678549&4%: ;88 %&'()5 %454%943:


18/82

!= /))- )6


19/82

TL&LHLX= !1)(7,1 C-0>,$(W 4$4#0#$; ;1)5(7?,-)$;0#)$ )6 0 $,5

C-0>,$( 910$"7= !* (7,*, 910$"7,* ;1)5L (7, >,>910$, 4*


20/82

NL EL a= !b,1 0

*7)1( #>, )6

C-0>,$(

,-)$;0#)$L (7,

,$(*

01, "0,$(* )6 !H/F

C-0>,$(* 01,

*,3,1,:?

:,


21/82

c P$)( *7)5$.

")C-4$ "0$ 9,(81$,: )_ 9'


22/82

@= \70( 5)8-: 9, (7, ,_,"( )6 0::4$; "'()"70-0*4$ () (74* `*'*(,>W= 2=

\70( 5)8-: 9, (7, ,_,"( )6 0::4$; 0 C-0>,$( *(094-4Z,1 P,=;=

d0*


23/82


24/82

&-0**,* )6 0"#$ 94$:4$; ,$( "1)**-4$+,1*.

@= /1)(,4$* (70( "1)**-4$+ 0"#$ 4$() -))*,-' )1:,1,: $,(5)1+*


25/82

2= /1)(,4$* (70( "1)**-4$+ C-0>,$(* 4$() 8$4910$,

>')*4$

T8$:-,* "0$ 0"( 0* 0 *4$;-, `01"74(,"(810- 8$4(W

0$: "0$ 9, >)3,:L


26/82

2= /1)(,4$* (70( "1)**-4$+ C-0>,$(* 4$() 8$4910$,


27/82

K= T4$:4$; 4D,:


28/82

K= T4$:4$; 4D,:


29/82

&-0**,* )6 0"#$ 94$:4$;


30/82

!"#$$%$ '( #)*+ ,-+.-+/ 01'2%-+$3

H= /1)(,4$* (70( 1,;8-0(, (7, 4$(,10"#)$ )6 >')*4$* 54(7 0"#$.

,=;= (1)')*4$G (1)4-' )6 >)-,"8-01 >)()1*=


31/82


32/82

THE MYOSIN SUPERFAMILY

Hodge and Cope, 2000

a8>0$

;,$)>,

,$"):,*

@K >')*4$"-0**,*

0$: 0

()(0- )6 Kf

>')*4$

;,$,*=


33/82

4%#.56'2'1

67'$-+ 8'9#-+ :21;)2;1%

?@?ABC2%+$-'+5>'$%3

6DE 6FE 6GHE 6GIE

6GJ

>%)K3

L! ,-+.-+/ @#-"

8-9%1-M#*'+

!#1/' ,-+.-+/

B+M79% #)*N-27

L')#"-M#*'+

L-/O2 !O#-+ P%?/?!#6Q


34/82

S)(01' *70:)5,: 1,


35/82

I7, 7,0:?>)()1 :)>04$* ")$(04$*!I/0*, 0$: 0"#$ 94$:4$; *4(,*

I7, (04- :)>04$ *,-6 0**)"40(,* 4$

0$#F


36/82

T4')*4$

I7, *>0--,*($)$>8*"-, >')*4$ cc

94,$( 4* 0

:4>,1=


37/82

I7, 7,0:F$,"+ :)>04$ )6 >8*"-,

>')*4$ ccG ,**,$#0- PX^&Q 0$:

1,;8-0()1' PS^&Q 01, "-0>04$ )6 >')*4$ 4* $,",**01' 0$:

*8g"4,$( 6)1 >)3,>,$(L


38/82

How would you demonstrate that the head/

neck domain of myosin II is necessary and

sufficient for actin filament movement?


39/82


40/82

/1)(,)-'#" 610;>,$(*

)6 >8*"-, >')*4$Fcc.

I7, >)()1 P[@Q :)>04$4* *8g"4,$( () ;,$,10(,

>)3,>,$(?6)1",=


41/82


42/82


43/82

6)1",


44/82

Lever Action of the Neck during

Power Stroke. (from Houdusse et al. 2000)


45/82


46/82


47/82

i))>F4$ 34,5 )6

*+,-,(0- >8*"-,


48/82

^)$;4(8:4$0- IXA *,"#)$ )6 *+,-,(0- >8*"-,


49/82

[01")>,1, 4$ 0 1,-0D,: *+,-,(0- >8*"-,


50/82

^)5 0$: 74;7 >0; 34,5* )6 "1)** *,"#)$ (71)8;7 *01")>,1, 0(

1,;4)$ )6 )3,1-0< 9,(5,,$ (74"+ 0$: (74$ C-0>,$(*= I74$

C-0>,$(* 01, 7,D0;)$0--'


51/82

j84"+F61,,Z, :,,< ,("7 34,5 )6 *01")>,1, 4$ 14;)1 *(0(,G

7,0:* )6 (74"+ C-0>,$( 01, 9)8$: () 0:k0",$( (74$ C-0>,$(*=


52/82

H40;10> )6 (7, 0"#$ >')*4$ )3,1-0< 1,;4)$ )6 (7, *01")>,1,


53/82

IXA 0$: :40;10>

)6 (7, *01")>,1,=


54/82

[01")>,1,

)1;0$4Z0#)$


55/82

S,-0D,: P()Q

*01")>,1,=


56/82

[-4:4$; C-0>,$( >):,- 6)1 >8*"-, ")$(10"#)$

l)(, (70( -,$;(7 )6 (74"+ 0$: (74$ C-0>,$(* :) $)(

"70$;, :814$; ")$(10"#)$


57/82

H40;10> )6

1,-0D,: 0$:

")$(10"(,:

*01")>,1,=


58/82

XD,$(0- X34:,$", 6)1 [-4:4$; C-0>,$( >):,-?"1)** 914:;,

7',$( -,$;(7* :) $)( "70$;, 0$: "70$;, 4$ )3,1-0,$(* 4* ")$*4*(,$( 54(7 *-4:4$; )6 0"#$ ()501:

(7, ",$(,1 )6 *01")>,1,=

2=

A,0*81,>,$( )6 (,$*4)$ 8*"-, :814$; ")$(10"#)$.1,3,0-,: (70( >')*4$F7,0:* 9,")>, :4*)1:,1,: :814$;

")$(10"#)$ Pc=,= 7,0:* 01, 4$ >)#)$Q=


59/82

A,0*81,>,$( )6 4*)>,(14" (,$*4)$ ;,$,10#)$ 9' >8*"-, C9,1* )6

:4_,1,$( -,$;(7. [01")>,1, -,$;(7 4* :41,"(-'


60/82

!( *01")>,1, -,$;(7* 57,1, *01")>,1, )1;0$4Z0#)$ 4* $)(


61/82

m 10' :4_10"#)$ )6 1,-0D,: >8*"-, 3,1*8* ")$(10"#$; >8*"-,

1,3,0-,: 0 -)** )6 )1:,1 )6 >')*4$ 7,0:* 0-)$; (7, (74"+ C-0>,$(

7,-4D. 4=,= 7,0:* 5,1, 4$ >)#)$=


62/82

S,;8-0#)$ )6 >8*"-, ")$(10"#)$G I (898-, )1;0$4Z0#)$ 6)1

P*01")4" 1,#"8-8>Q


63/82

IF(898-,F [S P*>))(7 XSQ ")$(0"( *4(, 4$ *01")>,1,


64/82

!"#)$ 910$,

*#>8-0(,* )


65/82

&02JF(1)


66/82

I$cF0"#$ 94$:4$; 1,*(104$* IA 61)> `60--4$;W 4$() ;1))3, )6

C-0>,$(= &02J 94$:4$; () I$& 4$:8",* I$&FI$c 94$:4$;L 1,*8-#$; 4$

0 *74b )6 IA 4$() ;1))3,=

n & 2J 94 :4 9 I & (7 Il ( 4 - 74b


67/82

n,$(L ,D')*4$ 94$:4$;

*4(,=


68/82

XD0>')*4$Fcc o0"#$ >,:40(,: *-4:4$; C-0>,$(

90*,: ")$(10"#-4(' 4$ $)$>8*"-, ",--*.

&)$(10"#)$ )6 "-,030;, 6811)5 :814$; "'()+4$,*4*

[(1,** C9,1 ")$(10"#)$ 4$ "8-(81,: ",--* 0$: 4$ 343)L

30*"8-01 ,$:)(7,-40- ",--*&)$(10"#)$ )6 0:7,1,$* k8$"#)$F0**)"40(,: 0"#$ 14$; 6)1

1,;8-0#)$ )6 #;7( k8$"#)$ ,094-4(' 4$ ,


69/82

A4()*4* 6)--)54$; 6,1#-4Z0#)$ )6 0 *,0 81"74$ ,;;= [


70/82

&'()+4$,*4* 4* >,:40(,: 9' 0 ")$(10"#-, 14$; )6 0"#$

C-0>,$(* 0$: >')*4$ cc C-0>,$(*


71/82

H40;10> 0$: IXA "1)** *,"#)$0- 34,5 )6 (7,

")$(10"#-, 14$;


72/82

&)$(10"#-, 14$;

)6 0"#$

C-0>,$(* P&SQ*,,$ 4$ "1)**F

*,"#)$ IXA

(71)8;7 14$;


73/82

[,"#)$ "8(


74/82

^)"0-4Z0#)$ )6 NF0"#$ P1,:Q

0$: >')*4$Fcc P;1,,$Q 4$ 0

:434:4$;


75/82

2F",-- *,0 81"74$ ,>91') 4$k,"(,: P",-- )$ 14;7(Q 54(7 0$ 0$#9):' (70(9-)"+* >')*4$Fcc >)()1 0"#34('= p4- :1)< P011)5Q >01+* 4$k,"(,: ",--=

l)(, (70( $8"-,01 :434*4)$ ")$#$8,* 98( "'()+4$,*4* 4* ")>


76/82

&-0**4" P61)> @fVqr*Q *-4:4$; C-0>,$( >):,- 6)1 "'()+4$,*4*. [4;$0-

61)> *9-' )6 >,>910$, (,(7,1,: 0"#$

C-0>,$(*G >')*4$ cc 4* 1,"184(,: 0$: 14$; ")$(10"(*=

Examples of Myosin Domain Organization


77/82

Examples of Myosin Domain Organization

Krendel and Mooseker, 2005


78/82


79/82

l,"+ -,$;(7 :,(,1>4$,* 0 >')*4$r*

*(,< *4Z,


80/82

Lever Action of the Neck during

Power Stroke. (from Houdusse et al. 2000)


81/82

N)1 ,D0>


82/82

c$ (7, `90"+501:*W >)()1L A')UL (7, -,3,1 01>

Unit+4+cytosskeleton+part+2+slides

Documents

Transcript of Unit+4+cytosskeleton+part+2+slides