Unit 8 Vocabulary
description
Transcript of Unit 8 Vocabulary
![Page 1: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/1.jpg)
Unit 8 Vocabulary1. micropipette- tool used to transfer small volumes of liquid
2. P20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used for our lab
3. P200 micropipette- transfers 20µL – 200 µL of liquid
![Page 2: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/2.jpg)
Unit 8 Vocabulary4. microliter (µL)- one millionth of a liter
5. milliliter (mL)- one thousandth of a liter
![Page 3: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/3.jpg)
Unit 8 Vocabulary6. microcentrifuge- machine
used to spin small volumes of liquids at high speeds; must be properly balanced
![Page 4: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/4.jpg)
Unit 8 Vocabulary7. microtube- a small plastic tube used in biotechnology that holds up to 1.5 - 2 mL
8. blank- a tube containing water used that is used to balance a microcentrifuge as needed
![Page 5: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/5.jpg)
Unit 8 Vocabulary
9. DNA fingerprinting- using enzymes to cut up the DNA for one or more genes in a pattern that can be matched to other samples in paternity and criminal cases
Crime:
Paternity:
![Page 6: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/6.jpg)
Unit 8 Vocabulary10. biotechnology- using biological techniques for practical applications, such as genetically modified foods
11. bioethics- the study of the moral concerns associated with biological research and applications
![Page 7: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/7.jpg)
Unit 8 Vocabulary12. electrolysis- the breaking of
H2O into H+ and OH- using electricity
13. electrophoresis- using an electrical current to separate DNA fragments according to length
![Page 8: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/8.jpg)
Unit 8 Vocabulary14. gel box- holds buffer and gel tray; has lid to protect user from electric current
![Page 9: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/9.jpg)
Unit 8 Vocabulary
15. agarose gel- Jell-O-like substance poured into gel tray; acts as a filter for DNA
![Page 10: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/10.jpg)
Unit 8 Vocabulary
16. What is the charge of DNA: positive, negative, or neutral? Negative
17. Why does the charge of DNA matter when running a gel? Since DNA is negative, it is repelled by the negative charge of the electrons flowing in the electricity, causing the DNA to move
![Page 11: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/11.jpg)
Unit 8 Vocabulary
18. allele- a version of a gene
19. Heterozygous- 2 different alleles for a gene
20. Homozygous- a pair of the same allele for a gene
![Page 12: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/12.jpg)
Unit 8 Vocabulary
21. restriction enzyme- cut DNA at certain sequences; used in DNA
fingerprinting; ex- HinDIII
![Page 13: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/13.jpg)
Unit 8 Vocabulary
21. buffer- prevents large changes in
pH22. loading dye- helps DNA to sink into well; helps you to track where DNA sample is
![Page 14: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/14.jpg)
Unit 8 Vocabulary
24. H2O- water 25. paternity- the state of being a
father
![Page 15: Unit 8 Vocabulary](https://reader036.fdocuments.net/reader036/viewer/2022062316/568168f2550346895ddffa96/html5/thumbnails/15.jpg)
Unit 8 Vocabulary
26. M0- mother’s DNA, uncut 27. M+- mother’s DNA, cut
28. B0- baby’s DNA, uncut
29. B+- baby’s DNA, cut
30. S0- suspect’s DNA, uncut
31. S+- suspect’s DNA, cut