Unit 8 Vocabulary

1. micropipette- tool used to transfer small volumes of liquid

2. P20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used for our lab

3. P200 micropipette- transfers 20µL – 200 µL of liquid

Unit 8 Vocabulary

4. microliter (µL)- one millionth of a liter

5. milliliter (mL)- one thousandth of a liter

Unit 8 Vocabulary6. microcentrifuge- machine

used to spin small volumes of liquids at high speeds; must be properly balanced

Unit 8 Vocabulary

7. microtube- a small plastic tube used in biotechnology that holds up to 1.5 - 2 mL

8. blank- a tube containing water used that is used to balance a microcentrifuge as needed

Unit 8 Vocabulary

9. DNA fingerprinting- using enzymes to cut up the DNA for one or more genes in a pattern that can be matched to other samples in paternity and criminal cases

Crime:

Paternity:

Unit 8 Vocabulary10. biotechnology- using biological techniques for practical applications, such as genetically modified foods

11. bioethics- the study of the moral concerns associated with biological research and applications

Unit 8 Vocabulary12. electrolysis- the breaking of

H2O into H+ and OH- using electricity

13. electrophoresis- using an electrical current to separate DNA fragments according to length

Unit 8 Vocabulary14. gel box- holds buffer and gel tray; has lid to protect user from electric current

Unit 8 Vocabulary

15. agarose gel- Jell-O-like substance poured into gel tray; acts as a filter for DNA

Unit 8 Vocabulary

16. What is the charge of DNA: positive, negative, or neutral? Negative

17. Why does the charge of DNA matter when running a gel? Since DNA is negative, it is repelled by the negative charge of the electrons flowing in the electricity, causing the DNA to move

Unit 8 Vocabulary

18. allele- a version of a gene

19. Heterozygous- 2 different alleles for a gene

20. Homozygous- a pair of the same allele for a gene

Unit 8 Vocabulary

21. restriction enzyme- cut DNA at certain sequences; used in DNA

fingerprinting; ex- HinDIII

Unit 8 Vocabulary

21. buffer- prevents large changes in

pH22. loading dye- helps DNA to sink into well; helps you to track where DNA sample is

Unit 8 Vocabulary

24. H2O- water 25. paternity- the state of being a

father

Unit 8 Vocabulary

26. M0- mother’s DNA, uncut 27. M+- mother’s DNA, cut

28. B0- baby’s DNA, uncut

29. B+- baby’s DNA, cut

30. S0- suspect’s DNA, uncut

31. S+- suspect’s DNA, cut