Unit 4.1 Biology

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    A2 level Biology notes

    Unit 4- Populations and the Environment

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    1. Populations ............................................................................ 31.1 Populations and ecosystems .................................................. 3

    1.2 Investing populations........................................................... 31.3 Variation in population size ................................................... 5

    1.4 Competition....................................................................... 71.5 Predation.......................................................................... 91.6 Human populations ........................................................... 10

    2. ATP.................................................................................... 122.1 Energy and ATP ................................................................ 12

    3. Photosynthesis ...................................................................... 143.1 Overview of Photosynthesis ................................................. 143.2 The Light- dependent reaction.............................................. 16

    3.3 The Light Independent Reaction ........................................... 173.4 Factors Affecting Photosynthesis ........................................... 18

    4. Respiration .......................................................................... 204.1 Glycolysis........................................................................ 204.2 Link reaction and Krebs cycle............................................... 214.3 The electron transport chain................................................ 23

    4.4 Anaerobic respiration ........................................................ 245. Energy and Ecosystems ............................................................ 25

    5.1 Food chains and Food webs.................................................. 255.2 Energy Transfer between Trophic Levels................................. 265.3 Ecological Pyramids........................................................... 27

    5.4 Agricultural Ecosystems ..................................................... 285.5 Chemical and Biological Control of Agricultural Pests ................. 29

    5.6 Intensive Rearing of Domestic Livestock................................. 316. Nutrient Cycles ...................................................................... 33

    6.1 The carbon cycle ............................................................... 33

    6.2 The greenhouse effect and global warming .............................. 346.3 The Nitrogen Cycle ............................................................ 356.4 Use of Natural and Artificial Fertilisers ................................... 36

    6.5 Environmental consequences of using nitrogen fertilisers ........... 367. Ecological Succession .............................................................. 37

    Succession ........................................................................... 37

    7.2 Conservation of Habitats ..................................................... 38

    8. Inheritance and Selection ......................................................... 398.1 Studying inheritance.......................................................... 398.2 Monohybrid Inheritance ..................................................... 408.3 Sex Inheritance and Sex Linkage ........................................... 41

    8.4 Co-dominance and Multiple Alleles ........................................ 428.5 Allelic Frequency and Population Genetics............................... 438.6 Selection......................................................................... 44

    8.7 Speciation ....................................................................... 45

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    1. Populations1.1 Populations and ecosystems

    Ecosystem- It is made up of all the interacting biotic and abiotic features in aspecific area

    Population- A group of interbreeding organisms of one species in a habitat.

    Community- All the populations of different organisms living and interacting

    in a particular place at the same time.

    Habitat -The place where a community of organisms lives.

    Ecological niche- All the biotic and abiotic conditions required for an

    organism to survive, reproduce and maintain a viable population. No 2

    species occupy exactly the same niche.

    1.2 Investing populations

    To study habitat often necessary count no

    individuals of species in given

    space

    This known as abundance

    Virtually impossible identify & count every organism

    Do so would time consuming & cause damage habitat being studied

    SO small samples usually studied in detail

    As long as samples representative of habitats as whole any conclusion drawn

    from findings will be validN

    osampling techniques used in study of habitat, these include:

    Random sampling using frame or point quadrats

    Systematic sampling along transects

    Quadrats

    Three factors to consider when using quadrats:

    Size quadrat use: depend upon size plants or animals being counted & how

    they distributed within area. Large species = larger quadrat

    Where species occurs in series group rather being evenly distributed

    throughout area, a large no

    small quadrats will give more representative

    results than small n

    o

    large onesN

    oof sample quadrats to record within the study area: larger n

    oof sample

    quadrats the more reliable results will be.

    Recording species w/in quadrat is time-consuming task, needs balance

    between validity results & time available.

    Greater no

    different species present in area studied = greater no

    quadrats

    required valid results

    Position each quadrat w/in study area: produce statistically significant

    results technique random sampling must used.

    Random Sampling

    Important sampling random avoid any bias in collecting data, avoiding bias

    ensures data obtained valid

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    A good method of random sampling is to:

    1. Lay out two long tape measures @ right angles, along 2 sides of studyarea

    2. Obtain series of coordinates by using random no taken from table orgrated by computer or calculator

    3. Place quadrat at intersection of each pair coordinates & record speciesw/in it

    Systematic sampling along transects

    Sometimes more informative measure abundance & distribution of species in

    systematic rather random manner

    Particularly important where some form transition in communities plants &

    animals take place

    Line transect comprise string or tape stretched across ground in straight line,

    any organism over which line passes is recorded

    Belt transect is strip, usually meter wide, marked putting second line parallelto first, species occurring w/in belt between lines recorded

    Measuring abundance

    Random sampling w/ quadrats & transects used obtain measures abundance

    Abundance no

    individuals of species w/in given space

    Can measured several ways, depending on size species being counted & the

    habitat, e.g.:

    Frequency: likelihood of particular species occurring in quadrat, e.g. a

    species occurs 15/30 quadrats frequency is 50%. Method useful where

    species is hard count, gives quick idea species present & general

    distribution. Does not provide info on density & detailed distributionspecies

    Percentage cover: an estimate area w/in quadrat that particular plant

    species covers. Useful where species is particularly abundant or diff

    count. Advantage is data collected rapidly & individual plants not need

    counted, less useful where organisms occur several overlapping layers.

    Obtain results, necessary ensure sample size is large, many quadrats used &

    mean all samples obtained. Larger the no

    samples, more representative

    community as whole will be results

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    1.3 Variation in population size

    A population is group interbreeding individuals of same species in a habitat

    Number individuals in population is population size

    All populations are different organisms that live & interact together areknown as a community

    Population Growth Curves

    Usual pattern of growth for natural population has 3 phases:

    1. Period of slow growth as the initially small number of individuals reproduce toslowly build up their number

    2. Period of rapid growth where the ever-increasing number of individuals continueto reproduce. The population size doubles during each interval of time

    3. Period when population growth declines until its size remains more or lessstable. The decline may be due to food supply limiting numbers or to increase

    population. Graph therefore levels out with only cyclic fluctuations due to

    variations in factors such as food supply or the population size of predators

    Population Size

    Imagine situation in which single algal cell, capable asexual reproduction,

    placed newly created pond

    Summer & so plenty light & temp water around 12oC, mineral nutrients been

    added to water

    These circumstances algal cell divides rapidly as all factors needed for growth

    population present

    Are no limiting factors

    In time, things change:

    1. Mineral ions used up as population becomes larger2. Population becomes so large that the algae at surface prevent light reaching

    those at deeper levels

    3. Other species introduced into pond, carried by animals or wind, and somethese species may use algae as food or compete for light or minerals

    4. Winter brings lower temp & light intensity of shorter durationAs result population slows and may possibly diminish, ultimately population

    likely reach relatively constant size

    There many factors (both biotic & abiotic) will affect the ultimate size

    Changes these factors will influence rate growth & final size population

    SUMMARY: no population continues grow indefinitely as certain factors limitgrowth, e.g. availability food, light, water, oxygen & shelter & accumulation

    toxic waste, disease & predators

    Each population has max size can be sustained over relatively long period,

    this is determined limiting factors

    Various limiting factors affect size population are of 2 types:

    1. Abiotic Factors: non-living part2. Biotic factors: activities living organisms

    Abiotic Factors

    Abiotic features that influence size population include:

    Temperature: each species has different optimum temp at which bestsurvive. Further away from optimum small population be supported. In plants

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    enzymes work more slowly & metabolic rate reduced. Populations grow

    more slowly. Temp above optimum, enzymes work less efficiently as

    gradually undergo deterioration. AGAIN population grows more slowly

    Warm-blooded animals, birds & mammals, maintain relatively constant body

    temp regardless external temp. Might think population growth & size unaffected

    temp. BUT further temp external environment gets from their optimum more

    energy expend to maintain normal temp. Leaving less energy individual growth &

    so mature more slowly & reproductive rate slows. Population size therefore

    smaller

    Light: as ultimate source energy for ecosystems, light basic necessity life.

    Rate photosynthesis increases as light intensity increases. Greater rate

    photosynthesis, the faster plants grow & more spores/seed produced. Their

    population growth & size therefore potentially greater. In turn population

    animals that feed on plants potentially larger

    pH: this affects action enzymes. Each enzyme has optimum pH at which

    operates most effectively. Population of organisms is larger whereappropriate pH exists & smaller, or non-existent where pH very different

    from optimum

    Water & Humidity: where water is scarce, populations are small & consistent

    only of species that are well adapted living in dry conditions. Humidity affects

    transpiration rates in plants & evaporation water from bodies of animals.

    Again, in dry air conditions, populations species adapted to tolerate low

    humidity will be larger than those with no such adaption.

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    1.4 Competition

    When 2 or more individuals share any resources (e.g. light, food, space,

    oxygen) that is insufficient satisfy all requirements fully = COMPETITION

    Where competition arises between same species: intraspecific competitionWhere competition arises between different species: interspecific

    competition

    Intraspecific Competition

    Intraspecific competition occurs when individuals same species compete w/

    one and other for resources

    It is availability of resources that determines size population

    Greater availability, larger the population, lower the availability smaller the

    population

    Examples

    Limpets competing for algae, main food. More algae available, largerlimpet population becomes

    Oak trees competing resources, in large population small oak trees some

    grow larger & restrict availability light, water & minerals to rest which die.

    In time population be reduced relatively few large dominant oaks

    Robins competing breeding territory. Female birds normally only

    attracted males w/ breeding territories, each territory provides adequate

    food for 1 family. When food scarce territories have become larger

    provide enough food. Therefore fewer territories given area = fewer

    breeding pairs = smaller population

    Interspecific CompetitionOccurs when individuals ofdifferentspecies compete for resources

    Where populations 2 species initially occupy same niece, 1 normally have

    competitive advantage

    Population this species gradually increase size while population other will

    diminish

    Conditions remain same, will lead complete removal one species

    Known as competitive exclusion principle where 2 species competing

    limited resources, one uses resources most effectively ultimately

    eliminate other

    No two species can occupy same niece indefinitely when resources limitingCormorant & Shags (sea birds) appear occupy same niece living &

    nesting same type cliff face & eating fish from sea

    Analysis food shows shags feed largely on sand eels & herring,

    cormorants eat mostly flat fish, gobies & shrimps

    Therefore occupy different niches

    Show how factor influences size of population necessary like birth rate &

    death rate of individuals to population

    E.g. increase food supply not necessarily mean more individuals; just result

    bigger individuals

    Therefore important show how factor, such as change in food supply, affectsnumber individuals in population

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    E.g. decrease food supply lead individuals dying starvation, resulting in

    reduction population

    Increase food supply means more likely individuals survive & so increased

    probability will produce offspring & population will increase

    Effect therefore takes longer influence population

    Application Effects Interspecific competition

    Many cases we suspect competition is reason variations in population.

    Practice difficult prove for number reasons:

    Many other factors influence population size, such as abiotic factors

    Casual like be established show that competition is cause of observed

    correlation

    Time lag in many cases competition & so population change may due

    competition took place years earlier

    Data natural population sizes hard to obtain & not always reliableHINT

    Although population of 1 species may increase as another decrease, this does not

    prove that this is due to direct competition between them. To be certain, it is

    necessary to establish a casual like for the observed correlation

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    1.5 Predation

    Predation occurs when one organism is consumed by another.

    Effect of predator-prey relationship on population size

    Predators eat their prey, thereby reducing the population of prey.With fewer prey available the predators are in greater competition with each

    other for the prey that are left.

    The predator population is reduced as some individuals are unable to obtain

    enough prey for their survival.

    With fewer predators left, less prey is eaten.

    The prey population therefore increases

    With more prey now available as food, the predator population in turn

    increases.

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    1.6 Human populations

    Human population size and growth rate

    Most of our history has been kept in check by food availability, disease, predators

    and climate. Two recent events have lead to an explosion in human population:The development of agriculture

    The development of manufacturing and trade that created the industrial

    revolution.

    Factors affecting the growth and size of human populations

    The basic factors that affect the growth rate of human populations are birth rate

    and death rate.

    Individual populations are further affected by migration, which occurs when

    individuals move from one population to another. There are two types:

    Immigration, where individuals join a population from outside.

    Emigration, where individuals leave a population.

    Population growth = (births + immigration) - (deaths + emigration)

    Factors effecting birth rate

    Economic conditions - countries with a low per capita income tend to have higher

    birth rates.

    Cultural and religious backgrounds - some countries encourage larger families and

    some religions are opposed to birth control.

    Social pressures and conditions - in some countries a large family improves social

    standing.

    Birth control - the extent to which contraception and abortion are used markedly

    influences the birth rate

    Political factors - governments influence birth rates through education and taxation

    policies.

    Birth rate = number of births per year x100

    total population in the same year

    Factors effecting death rate

    Age profile - the greater the proportion of elderly people in a population, the higher

    the death rate is likely to be.

    Life expectancy at birth - the residents of economically developed countries

    live longer than those of economically less developed countries.

    Food supply - an adequate and balanced diet reduces death rate

    Safe drinking water and effective sanitation - reduce death rate by reducing

    the risk of contracting water-borne diseases such as cholera.

    Medical care - access to healthcare and education reduces the death rate.

    Natural disasters - the more prone a region is to a drought, famine of disease

    the higher its death.

    War - deaths during wars produce an immediate drop in population and a

    longer term fall as a result of fewer fertile adults.

    Death rate = number of deaths per year x100 total population in the same

    year

    Population structure

    Age population pyramidThere are three typical types of population pyramids:

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    Stable population - where the birth rate and the death rate are in balance

    and so there is noincrease of decrease in the population size.

    Increasing population - where there is a high birth rate, giving a wider base to

    the population pyramid (compared to a stable population) and fewer older

    people, giving a narrower apex to the pyramid. This type of population is

    typical of economically less developed countries.

    Decreasing population - where there is a lower birth rate (narrower base of

    the population pyramid) and a lower mortality rate leading to more elderly

    people (wider apex to pyramid). This type of population occurs in certain

    economically more developed countries, such as Japan.

    Survival rates and life expectancy

    A survival curve plots the number of people alive as a function of time. Typically it

    plots the percentage of a population still alive at different ages but it can also be

    used to plot the percentage of a population still alive following a particular event,

    such as a medical operation or the onset of a disease.

    The average life expectancy is the age at which 50 per cent of the individuals in aparticular population are still alive. It follows that life expectancy can be calculated

    from a survival.

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    2. ATP

    2.1 Energy and ATP

    In most ecosystems the initial source of energy is the sun (light energy)Plants use solar energy for photosynthesis to make organic molecules, this

    takes place inside the chlorophyll inside chloroplasts which are mainly found

    in the spongy mesophyll of a leaf

    Carbon dioxide is taken in through the stomata

    Water is taken in through the roots

    Light is absorbed by the green pigment chlorophyll

    Oxygen is released into the atmosphere through the stomata

    Glucose is transported in solution for use or is stored as starch

    Energy is defined as the ability to do work

    Takes a variety of different forms- light, heat, sound, electrical, magnetic,

    mechanical, chemical and atomic

    Can only be changed form one form to another, cannot be created ordestroyed

    Measure in joules (J)

    Organisms need energy for

    Metabolism- reactions within living organisms

    Movement e.g. circulation of blood and locomotion

    Active transport- the net movement of particles against a concentration

    gradient across a plasma membrane

    Maintenance, repair and division of cells and organelles

    Production of substances e.g. enzymes and hormones

    Maintenance of body temperature in birds and mammals (endothermic

    organisms)

    The flow of energy through living organisms occurs in 3 stages: Light energy

    from the sun is converted by plants into chemical energy during

    photosynthesis, the chemical energy in the form of organic molecules is

    converted into ATP during respiration in all cells, this is then used to perform

    useful work

    Adenosine triphosphate has 3 phosphate groups, a 5 carbon ribose and an

    adenine group

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    The bonds between the phosphate groups are unstable and have a low

    activation energy so they are easily broken, when they are they release

    energy, it is the terminal phosphate that is removed.

    This is known as a hydrolysis reaction

    The reaction can also be reversed to made ATP from ADP through a

    condensation reaction

    The synthesis of ATP from ADP occurs in 3 different ways

    1. Photophosphorylation- takes place in the chlorophyll containing plantcells during photosynthesis

    2. Oxidative phosphorilation- occurs in the mitochondria of a plant andanimal cells during the process of electron transport

    3. Substrate level phosphorilation- occurs in plant and animal cells whenphosphate groups are transferred from donor molecules to ADP to make

    ATP

    not a good store of energy due to unstable bonds, but good as an immediate

    source of energy for same reason

    Each ATP molecule releases less energy than each glucose molecule therefore

    released in smaller more manageable amounts

    The hydrolysis of ATP to ADP is a single reaction that releases immediateenergy whereas the breakdown of glucose is a long series of reactions

    ATP is the source of energy for:

    (1)Metabolic processes polysaccharide synthesis from monosaccharide,polypeptide synthesis from amino acids and DNA/RNA synthesis from

    nucleotides

    (2)Movement- muscle contraction(3)Active transport- ATP provides energy to change the shape of the

    carrier proteins, allows molecules or ions to be moved against a

    concentration gradient

    (4)Secretion- needed to form the lysosomes

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    (5)Activation of molecules- lowers the activation energy of molecules sothey are more reactive so enzyme catalysed reactions can occur more

    readily

    3. Photosynthesis3.1 Overview of Photosynthesis

    Site of photosynthesis - the leaf

    Structure of the leaf

    is adapted to bring together the three raw materials of photosynthesis. (Water,

    carbon dioxide and light) and remove its products (oxygen and glucose). These

    adaptations include.

    A large surface area that collects as much sunlight as possible.

    An arrangement of leaves on the plat that minimises overlapping and so

    avoids the shadowing of one leaf by another.

    Thin, as most light is absorbed in the first few millimetres of the leaf and the

    diffusion distance is thus kept short.

    A transparent cuticle and epidermis that let light through to the

    photosynthetic mesophyll cells beneath.

    Long narrow, upper mesophyll cells packed with chloroplasts that collect

    sunlight.

    Numerous stomata for gaseous exchangeStomata that open and close in response to changes in light intensity

    Many air spaces in the lower mesophyll layer to allow diffusion of CO2 and

    oxygen.

    A network of xylem that brings water to the leaf cells and phloem that

    carries away the sugars produced in photosynthesis

    Outline of photosynthesis

    Capturing of light energy - by chloroplast pigments such as chlorophyll

    The light dependent reaction- in which light energy is converted into

    chemical energy. During the process an electron flow is created by the effect

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    of light on chlorophyll and this causes water to split (photolysis) into protons,

    electrons and oxygen. The products are reduced NADP, ATP and oxygen.

    The light-independent reaction - in which these protons (hydrogen ions) are

    used to reduce carbon dioxide to produce sugars and other organic

    molecules.

    Structure and role of Chloroplasts in photosynthesis

    Photosynthesis takes place inside the chloroplasts

    They are surrounded by a double membrane

    Inside the chloroplast membrane there are 2 distinct regions:

    - The grana- stacks of thylokoids where the light-dependent reaction

    takes place, contains the chlorophyll and can have tubular like structures

    to join them together called inter-granal lamellae

    - The stroma- fluid filled matrix where the light independent stage takes

    place, contain starch grains

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    3.2 The Light- dependent reaction

    Requires light

    Requires water

    Requires photosynthetic pigmentsOccurs in the thylokoids

    Light strikes chlorophyll and electrons are excited to a higher energy level

    where they are accepted by an electron carrier

    Photolysis occurs

    Electrons pass down the electron transfer chain to NADP firming ATP

    (Photophosphorylation- process by which ATP is made during the light

    reaction)

    Products are NADPH, ATP and O2

    Oxygen produced comes from water

    NAPDH and ATP are then using in the Calvin cycle (light independent reaction)

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    3.3 The Light Independent ReactionDoes not require light (can occur in both light and dark)

    Occurs in the stroma of the chloroplast

    Carbon dioxide from the atmosphere diffuses into the leaf through the

    stomata and dissolves in water around the walls of the mesophyll cells. It

    then diffuses into the plasma membrane, cytoplasm and chloroplast

    membranes into the stoma of the chloroplastIn the stroma, the co2 combines with the 5-carbon RuBP using the enzyme

    RuBisCo

    Combination produces 2 molecules of glycerate 3-phosphate

    ATP and NADPH from light dependent reaction are used to reduce the

    activated glycerate 3-phosphate to triose phosphate

    The NADP is re-formed and goes back to light dependent reaction

    Some triose phosphate are converted to useful organic substrates such as

    glucose

    Most are used to regenerate RuBP using ATP from the light dependent

    reaction

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    3.4 Factors Affecting PhotosynthesisLimiting Factors

    The law of limiting factors can be expressed as:

    At any given moment, the rate of a physiological process is limited by the factor

    that is at its least favourable value.Limiting factors of photosynthesis include light intensity, carbon dioxide

    concentration and temperature.

    Light intensity

    No light - no photosynthesis. The light phase does not take place.

    Increasing the light intensity to value A causes photosynthesis to increase.

    The more light the greater the light phase and the greater the production of

    ATP to the Dark Phase.

    At light intensity A the rate of photosynthesis reaches its maximum and levels

    off. Some factor other than light intensity is limiting the rate ofphotosynthesis: it may be low temperature, low carbon dioxide, low

    chlorophyll content or the enzyme system is deficient (enzymes at maximum

    turnover number).

    Light intensity A is known as the 'saturation point' - the value beyond which

    light intensity is not a limiting factor.

    The rate of photosynthesis remains constant at maximum beyond light

    intensity A. The Increase in light intensity has no effect on the new limiting

    factor so photosynthesis stays the same.

    Carbon dioxide

    concentration

    No carbon dioxide - no photosynthesis.

    Increasing the carbon dioxide concentration to value A causes photosynthesis

    to increase. The greater the supply of CO2, the faster the rate of enzyme

    activity

    At A the rate of photosynthesis reaches its maximum and levels off. Some

    factor other than CO2 is limiting the rate of photosynthesis: it may be low

    temperature, low light intensity

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    The rate of photosynthesis remains constant at maximum beyond A. Increase

    in CO2 has no effect on the new limiting factor so photosynthesis stays the

    same.

    Temperature

    At 0C the rate of photosynthesis is low. Enzyme activity is low.

    Photosynthesis is an enzyme-controlled process.

    Increasing the temperature to 30C increases the rate of photosynthesis.

    Enzyme activity increases.

    Maximum photosynthesis at 30C. Enzyme activity at it maximum - maximum

    collision frequency between native enzymes and substrates.

    Photosynthesis declines beyond 30C. Enzyme activity slowing due to

    denaturing of enzymes.

    No photosynthesis at 50C. No enzyme activity - enzymes are denatured.

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    4. Respiration4.1 Glycolysis

    The splitting of the 6C glucose molecule into two 3C pyruvate molecules.Occurs in the cytoplasm of the cells. Is an anaerobic process.

    Net production of 2 ATP molecules

    2 molecules of pyruvate produces

    2 molecules of reduced NAD produced (then used in the electron transport

    chain)Takes place in cytoplasm as glucose cannot enter the mitochondria due to

    size and enzymes used in the breakdown of glucose are found in the

    cytoplasm

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    4.2 Link reaction and Krebs cycleThe link reaction

    No energy is stored or removed in this reaction

    Occurs in the matrix of the mitochondria

    Pyruvate is converted into acytlycoenzyme A in this reaction

    This occurs twice so... 2 molecules of Acytlycoenzyme A, 2 reduced NAD and

    2 molecules of carbon dioxide are produced

    The Krebs cycle

    Occurs in the matrix of the mitochondria

    Provides a continuous support of electrons to fuel the electron transport

    chain

    Produces a SMALL amount of ATP

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    Occurs twice due to 2 acytylcoenzyme A molecules

    6 molecules of NADH produced (reduced NAD)

    2 molecules of FADH2 produced (reduced FAD)

    2 molecules of ATP produced

    4 molecules of carbon dioxide produced

    The NAD works with dehydrogenase enzymes that catalyse the removal of

    hydrogen ions and transfers then to other molecules such as hydrogen

    carriers involved in oxidative phosphorilation

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    4.3 The electron transport chainEnergy from hydrogen atoms removed from compounds can be used to make

    ATP

    Energy carried by electrons, from reduced coenzymes (reduced FAD and

    NAD) is used to make ATP, involves electron transport chain and

    chemiosmosis.

    Occurs on the cristae of mitochondria. Hydrogen from glycolysis is used.

    Reduced NAD and FAD are oxidised, releasing Hydrogen atoms. The H atoms

    are split into H+

    and e-.

    The regenerated NAD and FAD are reused in Krebs cycle.

    The electrons move along the electron transport chain (made up of 3

    electron carriers), in a series of oxidation-reduction reactions, losing energy

    at each stage.

    Some of this energy is used to combine an inorganic phosphate with ADP to

    make ATP. The remaining energy is released as heat.

    The protons accumulate in the space between the 2 mitochondrial

    membranes before they diffuse back into the matrix of the mitochondria

    through protein channels.

    At the end of the chain, the electrons combine with these protons and

    oxygen to form H2O.

    Oxygen is the final acceptor of electrons in the electron transport chain.

    Without oxygen acting as the final acceptor of electrons, the H

    +

    ions andelectrons would back up along the chain and respiration would come to a

    halt.

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    4.4 Anaerobic respirationOnly Glycolysis can occur in the absence of oxygen

    There are 2 forms of anaerobic respiration: alcoholic fermentation in plants

    and lactate fermentation in animals

    The production of ethanol if exploited in the brewing process

    Pyruvate is converted to ethanal by decarboxillationn reduced NAD is thenoxidised by the ethanal to give ethanol

    In animals, lactate is formed by the oxidation of reduced NAD by pyruvate

    The production of lactate regenerate the NAD and so Glycolysis can continue,

    a small amount of ATP is still produced to keep biological processes going

    An oxygen dept is created, lactate broken back down by oxygen at end

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    5. Energy and Ecosystems

    5.1 Food chains and Food websOrganisms can be divided into 3 groups depending on how they obtain

    their energyProducers- Photosynthetic organisms that manufacture organic

    substances using light energy, water and co2 by photosynthesis

    Consumers- Obtain their energy by consuming other organisms. Primary

    consumers feed directly of plants (producers) these are then consumed

    by secondary consumers and then tertiary consumers (usually predators

    can be scavengers or parasites)

    Decomposers- feed off dead organic matter to obtain energy which is

    trapped inside them. Majority of them fungi and bacteria (decomposers)

    and to a lesser extent by animals such as earth worms (detritivors)

    Food chains

    Describes a feeding relationship by showing the transfer of energy

    between producers and following consumers.

    Each stage is referred to as a trophic level

    Arrows represent the direction of energy flow

    Food webs

    Most organisms in a community do not just feed upon one animal, andone animal can be fed upon by many other animals, a food web shows

    SOME of the feeding relationships within the community

    Not all of the relationships can be shown as it would be too complex

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    5.2 Energy Transfer between Trophic LevelsMost energy is not converted by plants because, most of the suns light is

    reflected back into the atmosphere by clouds, not all wavelengths can be

    absorbed, light may not fall on the chlorophyll molecules and a limiting factor

    may stop the rate of photosynthesis e.g. low co2 levels

    The rate at which plants store energy is called net production:

    Net production= gross production respiratory loss

    Low % of energy transferred at each stage of the food chain is due to:

    1. Some of the organism is not eaten2. Some parts cannot be digested so lost in faeces3. Lost in excretory materials (urine)4. Lost as heat, maintaining body temperature5. Movement during hunting etc

    Most food chains dont have more then 4/5 trophic levels due to so much

    energy lost, insufficient energy to support more

    Total mass of organisms is less at higher trophic levels

    Total amount of energy stored is less at each trophic level

    Energy transfer (%) between each trophic level can be calculated by:

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    5.3 Ecological PyramidsPyramids are drawn to show changes in number, biomass or energy

    Pyramid of number

    Shows the number of organisms at each trophic level

    The more the wider the section of pyramid, the width of the block is

    proportional to the number of organisms present at each level

    Disadvantage: the range of numbers can be large

    Pyramid of biomass

    The biomass of all living organisms at each trophic level can be calculated by:

    Biomass = the number of individuals x mass of each individual

    Live mass can be used but gives unreliable results

    Not always practical/desirable to find the dry mass (mass of whales)

    Seasonal differences are not present and animal must be killed to get dry

    mass

    Units for biomass

    Pyramid of energy

    Shows the flow of energy through each trophic level of an ecosystem during a

    fixed period of time

    Always a fixed pyramid shape

    Units for energy

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    5.4 Agricultural EcosystemsMade up of largely domesticated animals and plants used to produce food

    for mankind

    Tries to ensure that as much of the available energy from the sun is

    transferred to humans

    Increases the productivity of the human food chain

    Net productivity = gross productivity respiratory loss

    Efficiency affected by the efficiency of the crop at photosynthesising and the

    area of the ground covered by the leaves of the crop

    Comparison of natural and agricultural ecosystems:

    Natural Ecosystem Agricultural Ecosystem

    Solar energy only Solar energy plus energy from food and

    fossil fuels

    Lower productivity Higher productivity

    More species diversity Less species diversity

    More genetic diversity within a species Less genetic diversity within a species

    Nutrients are recycled naturally within

    an ecosystem

    Natural recycling is more limited and

    supplemented by the addition of

    artificial fertilisers

    Populations are controlled by natural

    means (competition, climate)

    Populations controlled by both natural

    and use of pesticides and cultivation

    Natural climax community Artificial community prevented from

    reaching its climax

    Energy input

    To prevent an agricultural ecosystem from developing they remove all other

    species from a crop apart from the one they are growing

    To remove or suppress unwanted species requires an additional input of

    energy which comes in 2 forms, food for the farmers and fossil fuels for the

    machines.

    Productivity

    Additional energy input increases productivity, controlling photosynthesis

    within a greenhouse would also do this as maximum photosynthesis can be

    achieved (CO2 levels controlled, temp controlled, water controlled, minerals

    controlled ect)

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    5.5 Chemical and Biological Control of

    Agricultural PestsA pest is an organism that competes with humans for food or space,

    pesticides are poisonous chemicals used to kill pestsA pesticide should:

    1. Be specific- harmless to humans and other organisms2. Biodegrade- so it will break down into harmless substances in the

    soil but also needs to be chemically stable so has a long shell life

    3. Be cost effective4. Not accumulate- does not pass along food chain and harm other

    species higher up the food chain

    Biological control

    Controlling a pest by using its natural predator or parasites of the pestAim to control not eradicate- could be counterproductive, not enough pest

    for predator pest can increase in number again as predator dies

    Disadvantages:

    1. Do not act as quickly, so could be time between introductionand significant control

    2. Control may become a pest itself

    Biological Control Chemical Pesticides

    Very Specific Always have some effect on other

    species

    Once introduced, control organism

    reproduces itself

    Must be reapplied very

    expensive

    Pests do not become resistant Pests can develop genetic

    resistance so new pesticides

    have to be developed

    Risk that control organism becomesa pest as pest population is

    reduced control feeds on crops

    Risk of accumulation in species orpolluting nearby rivers

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    Integrated pest control systems

    Involves using all methods of pest control (chemical, biological and natural)

    to CONTROL the amount of pestInvolves:

    1. Choose a plant/animal that is immune as possible to the pest2. Manage the environment to provide habitats suitable for

    predators

    3. Monitor the crops for early signs of pests4. Remove pests manually is exceeds acceptable amount5. Use biological control if necessary and available6. Use pesticides as a last resort

    Such systems can be effective with minimum impact on the environment

    Pests reduce productivity in agricultural ecosystems (weeds compete with

    crop plants for water, minerals etc, insect can damage leaves of crops needed

    for photosynthesis/ in direct competition eating the crops themselves)

    Monoculture- a large area of land in which only 1 crop is grown, this enables

    pests to spread rapidly, pests may cause disease, animals become unfit for

    human consumption as do not grow rapidly which will lead to reduced

    productivity

    The effect of productivity is to balance the cost of pest control with the

    benefits it brings the problem is that the farmer has to balance the demand

    for cheap food while still making a living and the conservation of natural

    habitats so we can have food in the future.

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    5.6 Intensive Rearing of Domestic LivestockDesigned to produce the maximum yield of meat, eggs and milk at the lowest

    cost possible

    They do this by using methods to convert the smallest amount of food energy

    into the greatest amount of animal mass

    They do this my minimising there energy loss by keeping them in confined

    spaces to increase energy conversion rate, it does this because:

    1. Movement is restricted so less energy is used in muscle contraction2. Environment can be kept warm so not used to maintain body heat3. Feeding can be controlled for maximum growth4. Predators are excluded so no loss to other organisms in food web

    Other means include selective breeding of animals to produce varieties that

    are more efficient at converting food into body mass and using hormones to

    increase growth rates

    Main features of intensive rearing are:

    1. Efficient energy conversion2. Low cost3. Worst tasting food4. Less land is used leaving more natural habitats5. High density animal population more at risk to spread of disease but

    easier to isolate if this happens

    6.

    Animals are regularly given antibiotics to prevent spread of disease7. Over use of drugs lead to antibiotic resistance and can also alter the

    flavour of food or pass into the foods then into humans affecting their

    health

    8. Maintains a higher level of animal welfare but can lead to aggressivebehaviour from being in unnatural conditions

    9. Produces large concentrations of waste in a small area rivers andground waters may become polluted, pollutant gases can be

    dangerous and smell, larger have own waste facilities

    10.Reduced genetic diversity due to selective breeding11.High energy-conservation rates due to use of fossil fuels, CO2 levels

    released increased global warming

    Economic and environmental issues

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    Economic- desire for cheap food conflicts with the conservation of the

    environment

    Environment- reduced species diversity due to:

    1. removal of hedges and woodland2. creation of monocultures3. filling in ponds and draining marshes and other wetlands4. over-grazing of land preventing regeneration of woodland

    Indirect effect to reduce species diversity-

    1. use of pesticides and inorganic fertilisers2. escape of farm wastes into water courses3. absence of crop rotation leading to poor soil structure

    Conservation techniques include:

    1. maintaining existing hedgerows2. planting hedges as field boundaries3. maintaining existing ponds and where possible creating

    new ones

    4. leaving wet corners of fields rather than draining them5. planting native trees in low species diversity areas6. reduce use of pesticides using biological control where

    possible

    7. using organic rather than inorganic fertilisers8. using crop rotation with a nitrogen fixing crop9. creating natural meadows and using hay for silage10.leaving the cutting of verges and field edges until after

    flowering and seeds have dispersed

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    6. Nutrient Cycles

    6.1 The carbon cycle

    Shows how carbon moves through living organisms and the non-living

    environment.

    1. Carbon (Co2) is absorbed by plants by photosynthesis, becomingcarbon compounds in plants.

    2. Carbon is passed along the food chain through consumption.3. When organisms die, carbon compounds are digested by

    microorganisms and returned to the air as they carry out

    respiration.

    4. If any dead organic matter ends in places where there arent anydecomposers, their carbon compounds can be turned into fossil

    fuels. The carbon in these fossil fuels is released when they are

    burnt for fuel.

    CO2 concentration falls during the day as it is removed by plants as they carry

    out photosynthesis. It then increases at night as its no longer being removed

    by photosynthesis but all organisms continue to respire and add CO2.

    CO2 concentration decreases in the summer in some climates as it is when

    light intensity is greatest more photosynthesis can occur. More CO is

    removed as more plants are photosynthesising.

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    The Sun gives heat to

    Earth

    and the heat

    reflects from the

    surface into space

    but the greenhouses

    gases stops the heat

    from reflecting away

    so the heat bounces

    back in.

    6.2 The greenhouse effect and global

    warmingThe greenhouse effect is a natural process that occurs all the time without it

    the average temperature on earth would be -18 degrees Celsius, the gasesthat surround the earth in the atmosphere trap the heat from the sun

    keeping it warm at 17 degrees Celsius

    The most important greenhouse gas is CO2 because it remains in

    the atmosphere for much longer than other

    greenhouse gases, 50-70% of global warming is due

    to CO2.

    Human activities increases the amount of carbon

    dioxide in the atmosphere enhancing the greenhouse

    effectMethane is also a greenhouse gas which is produced

    when decomposers break down the dead remains of

    organisms or then microorganisms in the intestines of

    primary consumers digest the food that has been

    eaten

    Global warming is where the mean temperature of the Earth has increased

    Consequences of global warming:

    1. Melting of polar ice caps which could cause theextinction of some plants and animals and also a rise in

    sea level

    2. Rise in sea level could cause flooding, salt water wouldextend further up rivers making cultivation of crops

    difficult

    3. Higher temperatures and less rainfall lead to failure ofcrop growth in some areas, distribution of wild plants

    change and so animal distribution would change

    4. Greater rainfall and storms in some areas cause changein distribution of plants and animals

    5. Life-cycles and populations of insect pests would changeas they adapt, tropical diseases could then spread

    further up north as the insects migrate

    Could also benefit as increased rainfall would fill reservoirs, increased

    temperature grow crops in places where originally too cold, rate of

    photosynthesis increase, may be possible to harvest twice a year

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    6.3 The Nitrogen CyclePlants and animals need nitrogen to make proteins and nucleic acids

    (DNA/RNA)

    Although the atmosphere has 78%, can't use it in that form, need saprobiotic

    bacteria to convert it into nitrogen compounds first.Nitrogen Cycle includes:

    1. Nitrogen fixation

    2. Ammonification

    3. Nitrification

    4. Denitrification.

    1. Nitrogen Fixation (Can also happen when lightning passes through the

    atmosphere)

    Nitrogen gas in the atmosphere is turned to ammonia by nitrogen-fixing

    bacteria

    Free-living nitrogen fixing bacteria reduce gas to ammonia which is then usedto manufacture amino acids. Nitrogen rich compounds released when they

    decay

    Mutualistic bacteria e.g. Rhizobium is found in root nodules of leguminous

    plants. e.g. peas, beans

    They form a mutualistic relationship with the plants- they provide the plant

    with nitrogen compounds and the plant provides them with carbohydrates

    2. Ammonification

    Nitrogen compounds from dead organisms are turned into ammonium

    compounds by decomposers

    Animal waste also contains compounds and are turned into ammonium3. Nitrification

    This is the conversion of ammonium ions to nitrate ions by nitrifying bacteria,

    to be used by the plant.

    Firstly nitrifying bacteria oxidise ammonium ions to nitrite ions (N02-)

    Secondly other nitrifying bacteria oxidise nitrite ions to nitrate ions (N03-)

    The bacteria require oxygen, so soil with lots of air spaces by ploughing and

    good drainage so air spaces are not filled with water is needed.

    4. Denitrification

    Nitrates in the soil are converted into nitrogen gas by denitrifying bacteria

    they use nitrates in the soil to carry out respiration and produce nitrogen gas

    Happens under anaerobic conditions - no oxygen e.g. waterlogged soil.

    Parts of the cycle can be carried out artificially on an industrial scale. Haber

    process produces ammonia from atmospheric nitrogen to make fertilisers.

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    6.4 Use of Natural and Artificial FertilisersAll plants need mineral ions, especially nitrogen

    Intensive food production makes large demands on soil because mineral ions

    are continually being taken up by crops grown there

    In natural ecosystems the minerals are returned when the plant is broken

    down my microorganisms on its death

    In agricultural the plants are harvested and transported for consumption and

    are rarely returned to the same area, making it necessary to replenish these

    minerals or it will become a limiting factor to the plants growth

    To do this 2 different types of fertilisers are added:

    1. Natural (organic) fertilisers- consist of dead and decayingplants and animals as well as animal waste (manure and bone

    meal)

    2. Artificial (inorganic) fertilisers- mined from rocks and depositsthen converted into different forms and blended to give the

    appropriate amount of mineral needed for the land (NKP

    fertilisers)

    Plants need these minerals for grown e.g. nitrogen to make proteins and

    DNA, when available plants are more likely to develop earlier, grow taller and

    have a greener leaf area, this increases rate of photosynthesis and so

    increases productivity

    6.5 Environmental consequences of using

    nitrogen fertilisersReduced species diversity can occur- nitrogen rich soils favour the growth of

    grasses, nettles and other rapidly growing species which causes more

    competition for other species which then die out and so reduces species

    diversity.

    Leaching- when water soluble compounds in soil are washed away, e.g. byrain / irrigation systems, into nearby ponds and lakes.

    If nitrogen fertiliser is leached it can cause eutrophication:

    1. Nitrates leached from fertilised fields stimulate growth of algae in ponds

    etc.

    2. Large amounts of algae block light from reaching plants below

    3. Plants die as they are unable to photosynthesise

    4. Bacteria feed on the dead plant matter

    5. Increased numbers of bacteria reduce the oxygen concentration in water

    by carrying out aerobic respiration

    6. Fish etc. die as there isn't enough dissolved oxygen

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    Organic manures, animal slurry, human sewage, ploughing old grass land and

    natural leaching can also cause eutrophication but artificial is main cause.

    7. Ecological Succession

    7.1SuccessionSuccession --> term to describe changes taking place over time

    1st Step is --> colonisation of an inhospitable environment by organisms -->

    called pioneer species --> their features suit them because they:

    1. rapidly germinate seeds2. reach isolated areas easily3. have the ability to photosynthesise4. have the ability to fix nitrogen5.

    have tolerance to extreme conditions

    Succession takes place in a series of stages --> at each stage certain species

    can be identified which change the environment --> therefore the

    environment becomes more suitable for other species --> these other species

    out compete current species --> this forms a new community

    During any succession, common features are:

    1. the non-living environment becomes less hostile which leads to -->2. greater number and variety of habitats which produce -->3. increased biodiversity which lead to -->4. more complex food webs5. increased biomass

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    7.2 Conservation of Habitats

    Conservation is the management of the Earth's natural resources so that maximumuse of them can be made in the future.

    Main reasons for conservation:

    Ethical- other species have existed longer and so should be allowed to co-

    exist

    Economic- long term productivity is greater if ecosystems are maintained in

    their natural balanced state

    Cultural and aesthetic- Variety add interest to our every day lives

    Ways to manage succession:

    1. Animals left to graze on land, so larger plants can't establish themselves and

    vegetation kept low

    2. Managed fires are lit, after fires secondary succession will occur on the moorland,

    so the pioneer species growing back will be the species that is being conserves e.g.

    heather

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    8. Inheritance and Selection

    8.1 Studying inheritance

    Dominant Allele: allele that is always expressed in the phenotype.

    Recessive Allele: allele that is expressed in the phenotype in the absence of a

    dominant allele.

    Co-dominance: both alleles are dominant and are expressed in the

    phenotype.

    Genotype: constitution of an organism comprising all the genes possessed by

    an individual.

    Phenotype: characteristics of an organism, often visible, resulting from the

    genotype and the effects of the environment.Heterozygous: having two different alleles for a given gene.

    Multiple Alleles: when a gene has more than 2 allelic forms

    Homozygous: having two dominant/recessive alleles present for a given gene.

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    8.2 Monohybrid InheritanceRepresenting genetic crosses

    Choose a single letter to represent each characteristicChoose the first letter of one of the contrasting features

    Choose the letter in which the higher and lower case forms differ in shape as

    well as size so they cannot be confused

    Higher letter represents dominant gene, lower for recessive gene

    State the gametes produces by each parent, indicating meiosis

    Use a punnet square to show the result of the random crossing of gametes,

    label male and female

    State the phenotypes of each different genotype and indicate the number of

    each type. Always write the higher case letter firstInheritance of pod colour in peas

    Another example of a monohybrid cross is a person with Huntington disease, this is a

    dominant gene: coded for by protein Huntington

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    A similar cross can be done for cystic fibrosis which is the recessive gene coded for

    by the protein CFTR

    8.3 Sex Inheritance and Sex LinkageSex is determined by chromosomes rather than genes

    As females on have x and males have either x or y males determine the sex of

    a child (xx for female, xy for male)

    Sex Linkage- Haemophilia

    A gene that is carried on the x or y chromosome is said to be sex linked

    Carried on the X chromosome, males either have the disease or dont but

    women can be carriers

    Males can therefore only obtain a disease from their mothers as the gene is

    not carried on the y chromosome they inherit from their fathers but the x

    chromosome from the mother, if she does not have the disease but the son

    does then she would be a carrier and so heterozygous for the condition

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    8.4 Co-dominance and Multiple AllelesCo-dominance occurs when both alleles are dominant so both are expressed

    within the phenotype

    E.g. a plant that codes for red and white flowers, both are dominant so the

    colours would be:

    1. Homozygous for red = red2. Homozygous for white = white3. Heterozygous = pink

    C= colour and then R= red and W= white:

    Multiple alleles

    Inheritance of the ABO blood group is an example of this

    3 genes carried on the I (immunoglobulin gene), which lead to the different

    production of different antigens on the surface of red blood cells

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    8.5 Allelic Frequency and Population GeneticsGene pool- all of the genes of all the individuals of a population at any one

    time

    Allelic frequency- the number of times a gene appears within the gene pool

    Example:

    Cystic fibrosis- C- dominant allele which codes for normal production of

    mucus

    c- Recessive allele which codes for thinker production of mucus and cystic

    fibrosis

    Pairs of alleles for cystic fibrosis have 3 different combinations:

    1. CC- heterozygous dominant2. cc- Homozygous recessive3. Cc / cC- Heterozygous

    The total number of alleles is said to be 1.0, in a population if no one had

    cystic fibrosis then the frequency of the gene c would be 0.0 whereas the

    frequency of the gene C would be 1.0. If everyone was heterozygous then the

    frequency of C or c would be 0.5

    The Hardy-Weinberg Principle

    A mathematical equation can be used to calculate the frequency of alleles

    Principle states that the proportion of dominant and recessive alleles stays

    the same from generation to generation if:(1)No mutations arise(2)The population is isolated(3)There is no selection(4)The population is large(5)Mating within the population is random

    Let the frequency of allele A = p and the frequency of allele a = q

    P + q = 1.0

    4 possible arrangements (AA, Aa, aA and aa) the frequency of all 4 added

    together = 1.0Therefore:

    If 1 in 25000 people have a (recessive) then aa= 1/25000 therefore q squared

    = 0.00004

    If p + q = 1.0 and q is then equal to 0.00063, p = 0.9937

    To calculate heterozygous you then use 2pq = (2 x 0.9937 x 0.0063) = 0.0125

    So 125 in 10 000 carry the allele for the character

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    8.6 SelectionReproductive success and allele frequency

    All organisms produce more offspring then can be supported

    Despite overproduction most population remain constant

    There is competition between members of a species to survive

    Within the population thee will be a wide variety of alleles in the gene pool

    Some will possess the genes which make them better able to survive

    These individuals will obtain he available resources and grow more rapidly as

    a result will have more successful breeding and offspring

    The more successful then pass on their genes

    The ones with advantageous alleles will then compete better and will

    reproduce

    The number of individuals with the advantageous alleles will increase

    Over time, the frequency of the allele increases

    The advantages will vary due to environment

    Types of selection

    Selection is the process in which organisms that are better adapted will

    survive and breed

    Different environmental conditions favour different characteristics within a

    population

    Selection that favours individuals in one direction from the mean population

    is called directional selectionSelection that favours the mean population is called stabilising selection

    Directional selection

    Environmental conditions change so phenotype needed to survive changes

    New individuals become more adapted to survive at one end of the spectrum

    and so over time the mean changes to suit the new phenotype

    This results in phenotypes at one extreme being favoured and the other

    being favoured against

    Stabilising selection

    Environmental conditions remain the sameMean are favoured, extremes are favoured against

    Eliminates extremes

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    8.7 SpeciationSpeciation is the evolution of new species from an existing one

    Species- a group of individuals with similar genes that can produce fertile

    offspring

    If 2 populations become isolated in some way, there is no longer a flow of

    alleles, the environment with each group may differ and so one type of allele

    frequency may change in time the gene pools will become so different that

    they are no longer the same species

    Geographical isolation:

    Occurs when a physical barrier prevents 2 populations from breeding with

    one another e.g. rivers, mountains and deserts

    Example in a forest

    Continued adaptationsLeads to evolution

    Of new species Y and Z