Unique xylanase scientifically designed for consistent premium performance

Unique xylanase scientifically designed for consistent premium performance

Scientifically Designed and Developed

Xylamax® is a unique high quality xylanase enzyme that helps producers improve animal

nutrition and gut health in a safe and sustainable way. It has been scientifically developed to consistently deliver premium performance across a wide range of feed formulations, production

environments and practices used around the world.

An endo-1,4-beta-xylanase, Xylamax was developed by BRI scientists from a unique gene found in a naturally occurring fungal micro-organism. The primary function of xylanase enzymes is to break down

xylans, a type of non-starch polysaccharide (NSP) found in the cell walls of corn and other grains, so that they are more easily digested by poultry. Xylamax performs this function extraordinarily well because of its

unique protein structure and pH activity profile.

Xylamax Mode of ActionResearch confirms that 450-500 kcal/kg of valuable energy in a typical poultry diet is not digestible. The addition of

enzyme feed additives has been shown to make up to 30% (135 kcal/kg) of this energy digestible (Cowieson, 2010). Xylans are the most prevalent type of NSP in poultry feed grains, representing up to 43% of the total NSP content.

Xylanase enzymes hydrolyze glycosidic bonds in xylans that make up cell walls, resulting in the release of the encapsulated nutrients.

Xylamax Activates Nutrient Release by Breaking Down Components of Grain Cell Walls

Nutrients Trapped InsideGrain Cell Wall*

*Illustration

Nutrients Released FromDigested Grain Cell Wall*

Negative Impact of Xylans in Animal Feed

Digestibility of a 3,000 kcal/kg diet

Collins and Moran, 1998Watt et al, 2009Cowieson, 2010Roberts et al, 2015

UndigestedEnergy

450 kcal/kg DigestedEnergy

2,550 kcal/kg

Unique Protein StructureMaximizes Energy Release

Xylans occur in two types, water soluble and water insoluble. There are two families of xylanase enzymes used in animal feed, GH10 which primarily hydrolyzes soluble xylans and GH11 which more effectively hydrolyzes both soluble and insoluble xylans (Berrin and Juge, 2008).

To maximize release of encapsulated nutrients in feed grains, the correct type of xylanase enzyme is required. For example, 98% of the xylan content in corn is insoluble. In order for poultry to digest a typical corn-SBM diet, a xylanase which targets insoluble xylans (GH11), such as Xylamax, is required.

Xylamax is a unique GH11 xylanase with an extended enzymatic site which allows for greater catalytic activity.

Typical GH10 Xylanase Typical GH11 Xylanase

Xylamax

GH11 Xylanase Hydrolyzes Both Soluble and Insoluble Xylans In Corn-SBM Diets

8

6

4

0

% DryMatter

Soybeanmeal

Barley Rye Wheat Corn

Insoluble Xylan Soluble Xylan

Xylamax Overlayed on Typical GH11 Xylanase

(Choct, 1997)

Unique pH Activity Profile Increases Nutrient Absorption

The Impact of Enzyme pH and Temperature Profiles Feed digestion occurs mainly in the small intestine of poultry (duodenum, jejunum, ileum) as evidenced by the amount of time the digesta material remains in these areas of the bird’s gut. The majority of absorption of nutrients also occurs in the small intestine due to the presence of a higher number of villi, the site of nutrient absorption. For any enzyme to deliver maximum results, it needs to work optimally at the 5.7-7.0 pH range occurring in the small intestine (Sturkie, 2000). Xylamax is the highest activity enzyme in this range. Xylamax also exhibits activity in excess of 75% within the 40.6-43.0°C temperature range of the poultry gut.

The Impact of Digesta Viscosity A highly viscous composition of the digested material in the bird’s intestine causes a decrease in the passage rate through the gastrointestinal tract. As a result, nutrient diffusion and absorption in the foregut is reduced, and unabsorbed nutrients are allowed to enter the hindgut where they facilitate the proliferation of detrimental bacteria. High digesta viscosity further impairs poultry nutrition by reducing feed intake.

Xylamax Accelerates Nutrient Absorption by Reducing Digesta Viscosity and Improving Passage Rate

12

10

8

6

4

2

0

Viscosity(cP)

Control

Wheat Diet

Control + Xylamax

4.5

4.0

3.5

Viscosity(cP)

Control Control + Xylamax

Corn Diet

Optimum Activity Where Majority of Digestion Takes Place Xylanase Activity x Temperature Profile

120

100

80

60

40

20

0

180

150

120

90

60

30

0

MaximumEnzymeActivity

(%)

Sturkie’s Avian Physiology, 2000

RetentionTime

(minutes)

120

100

80

60

40

20

0

PercentMaximumActivity

(%)

Temperature (˚C)pH Color Scale

3 4 5 6 7 8 9 10

Copyright© 2015 BioResource International, Inc.

Crop

Proventriculus

Gizzard

Duodenum

Jejunum and Ileum

Colon

Cecum

Hindgut

Foregut

Proventriculusand Gizzard

Crop

Cecum

Duodenum

Jejunum and IleumXylamaxLeading SingleXylanase

Leading CocktailEnzyme

Colon

(NC, USA 2014) (NC, USA 2014)

20 25 30 35 40 45 50 55 60

Poultry Temperature Range40.6 - 43.0˚C

105.0 - 109.4˚F

Xylamax

Leading SingleXylanase

Leading CocktailEnzyme

Naturally Improves Gut Health

Not only do undigested xylans limit nutrient availability, increase digesta viscosity and reduce nutrient absorption and utilization by the bird, they may also contribute to the growth of intestinal pathogens that thrive on undigested nutrients in the feed. Proliferation of these pathogens is associated with compromised morphological development of the gut mucosa, pathogen-induced gut inflammation, oxidative stress and tissue damage. By effectively degrading xylans found in grains, Xylamax improves the overall gut health of animals.

Impact on Mucosal Morphology and Growth of PathogensBy effectively attacking the detrimental effects of xylans, Xylamax limits growth of pathogens in the intestine while improving the bird’s nutrient absorption and utilization because of higher nutrient digestibility and improved gut function, as indicated by increased villi height in the presence of Xylamax in feed.

Decreased Severity of Necrotic LesionsNecrotic Enteritis, characterized by Clostridium perfringens, costs the global poultry industry about $2 billion annually (Ducatelle and Immerseel, 2010). Necrotic Enteritis causes necrotic lesions in the intestinal tract, poor digestion, reduced weight gain, increased FCR, and even death. In a 42-day pen trial, birds were fed a typical corn-soy diet and were challenged with C. perfringens (107/bird). Necrotic lesions in birds supplemented with Xylamax were significantly less severe than in birds that were not supplemented with the enzyme, contributing to the overall gut health of the birds.

(NC, USA 2015)

Xylamax Decreases Necrotic Lesions

LesionScores

0.700.600.500.400.300.200.100.00

Control Control + XylamaxDay 42

(NC, USA 2015)

Xylamax Increases Villi Height

VilliHeight(um)

620

615

610

605

600

595Control Control + Xylamax

(NC, USA 2015) (NC, USA 2015) (NC, USA 2015)

Xylamax Reduces Crypt Depth

CryptDepth(um)

120

115

110

105

100

95Control Control + Xylamax

Xylamax Increases Height:Depth Ratio

Ratio(um:um)

6.0

5.5

5.0

4.5Control Control + Xylamax

Xylamax Improves Mucosal Morphology

Nutrients TrappedInside Cell Wall*

*Illustration

Bifidobacterium(Conn, 2009)

Nutrients ReleasedFrom Cell Wall*

XOSPrebiotic Effect

Increases Growth ofBeneficial Bacteria

Xylanase

Produced VFAs(volatile fatty acids)

Inhibits Pathogen Growth/EncouragesGood Bacteria Growth

Generates Energy

Naturally Improves Gut Health

Reduction of Oxidative Stress and Mucin SecretionA healthy gut can be measured not only by mucosal morphology but also by indicators of oxidative stress, such as malondialdehyde (MDA). Increases in MDA have been associated with suppressed gut development, reduced growth performance and poor feed efficiency in broilers. Supplementation of Xylamax in broiler feed has been shown to reduce MDA concentrations in serum and mucosa.

Creation of Prebiotic EffectIn addition to limiting the growth of pathogens through improved nutrient digestibility, Xylamax helps maintain the healthy microbiota by producing xylo-oligosccharides (XOS), which preferentially promotes the growth of beneficial bacteria, such as Bifidobacterium and Lactobacillius in the foregut. This prebiotic effect offers the host additional protection against potential pathogenic challenges, allowing more consistent performance in various commercial settings.

MDAConcentration

(ug/g)

0.60

0.55

0.50

0.45

0.40Control Control + Xylamax

Xylamax Reduces Mucosal MDAConcentration

(NC, USA 2015)

Versatile Solution, Consistent Performance

Xylamax delivers consistently high performance across a range of feed ingredients as shown in animal feeding trials conducted by internationally recognized independent poultry research institutions. All BRI products are manufactured in accordance with world class GMP and FAMI-QS standards to ensure a consistent high quality product from batch to batch.

Flexible Feed Formulation Strategies

Birds treated with Xylamax are heavier on average than birds not treated with an enzyme. Furthermore, a larger proportion of the birds are closer to the average weight, improving flock uniformity. Xylamax treatment contributes to a heavier, less variable flock.

Xylamax Increases Energy Digestibility by 152 kcal/kg

200180160140120100

80604020

0

Increasekcal/kg

Corn Diet Wheat Diet DDGS Diet

Average152

TX, USA 2013 TX, USA 2014 NC, USA 2014 HCMC, Vietnam 2016

Reformulation Strategy - Recommended

Reformulate diet to take advantage of additional energy release

Use Xylamax matrix value calculator to determine maximum matrix value based on feed formulation

On-top Strategy Add Xylamax on top of current diet to improve FCR by 4-6 points based on feed formulation and Xylamax dose

Xylamax Improves FCR by 4.1 Points

876543210

Improvementin FCR

Corn Diet Wheat Diet Sorghum Diet DDGS Diet

Average4.1

USA INDIA PERU

Xylamax Improves Average Bird Weight and Flock Uniformity

30

25

20

15

10

5

02400 2500 2600 2700 2800 2900 3000 3100 3200 3300 3400 3500 3600 3700 3800 39002200 2300

Without Xylamax With Xylamax(NC, USA 2013)

(See references on back page)

WithoutXylamax

AverageWeight(grams)

StandardDeviation(grams)

3064

257.4 203.3

3129

WithXylamax

Weight (grams)

Numberof Birds

BRAZIL THAILAND

Copyright© 2017 BioResource International, Inc. or its affiliates, all rights reserved. The BRI logo and all products denoted with ® or ™ are registered trademarks or trademarks of BioResource International, Inc. or its affiliates. Information regarding the legal status of this product may be obtained on request. The information contained in this publication is based on our own research and development work, or work conducted by third parties, and to our knowledge is reliable. Always read the label and product information before use. Users should conduct their own tests to determine the suitability of our products for their specific purposes. Statements contained in this publication should not be considered as, and do not constitute a warranty of any kind, expressed or implied, and no liability is accepted for the infringement or misuse of any patents, or other intellectual property of third parties.

4222 Emperor Blvd, Suite 460, Durham, NC USA 27703+1-919-993-3389 • sales@BRIworldwide.com

www.BRIworldwide.com

Designed, Developed and Tested by:

Recent Xylamax Animal Trials

Specifications

Xylamax 100 Xylamax 500

50-100g/MT dose 250-500g/MT dose

150,000 XU/g activity 30,000 XU/g activity

10kg bag 25kg bag

Form: Uncoated powder feed additive

Thermostability: Xylamax is thermostable at pelleting temperatures up to at least 85°C

Dosage:

Energy Release: Xylamax provides up to 130 kcal ME/kg in corn-based poultry diets, and up to 150 kcal ME/kg in wheat-based poultry diets.

Research Institutions Country Year Principal Investigator

Nong Lam University, HoChiMinh City Vietnam 2016 D. DongBangkok Animal Research Center, Samut Praken Thailand 2016 T. SooksridangUniversidad Nacional Mayor de San Marcos, Veterinary Medicine Faculty, Avian Pathology Laboratory experimental farm, San Borja district, Lima Peru 2016 E. IcocheaFederal University of Viçosa, Center for Agricultural Sciences, Dept of Animal Science – Viçosa, Minas Gerais Brazil 2016 H. RostagnoNC State University USA 2016 S. Kim Annam Feeds Research and Development Trial Farm India 2016 C. Chandrasekaran Universidade Federal Do Rio Grande Do Sul Brazil 2015 S.VieraNC State University USA 2014 P. BiggsNC State University USA 2014 J. GrimesIndian Council of Agricultural Research India 2014 R. RaoTexas A&M University USA 2014 J. LeeNC State University USA 2014 P. BiggsIndian Council of Agricultural Research India 2014 R. RaoNC State University USA 2014 J. TyusTexas A&M University USA 2013 J. Lee