Ultra Fast Speed High Reliability User-Friendly€¦ · Ultra Fast Speed Fusion of Ultra Fast MRM...
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Ultra Fast Speed Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible High Reliability Robust System Provides Long-term Data Stability UFsweeper TM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range User-Friendly Easy Maintenance for Minimized Instrument Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation
Transcript of Ultra Fast Speed High Reliability User-Friendly€¦ · Ultra Fast Speed Fusion of Ultra Fast MRM...
Ultra Fast Speed
Fusion of Ultra Fast MRM and Ultra Fast Polarity SwitchingAn Ultra Fast Scan Speed of 15,000 u/sec500 MRMs per Second, the Most Ever Possible
High Reliability
Robust System Provides Long-term Data StabilityUFsweeperTM Technology Dramatically Minimizes Cross TalkExcellent Linearity with Wide Dynamic Range
User-Friendly
Easy Maintenance for Minimized Instrument DowntimeSingle-vendor Solution Provides Seamless OperationQuantitation Browser for Effective Multi-analyte Quantitation
Unit-205385>107Vitamin D3Unit-155431>165Vitamin EUnit-205473>207Vitamin E acetate
Unit-355397>69Vitamin D2
Unit-305451>187Vitamin K1Unit-255269>93Vitamin A palmitate
Unit-255269>93Vitamin A
MS2 resolution
CE (eV)
Dwell time(ms)TransitionTransition
MRM Mode Parameters
100100--50000ppb50000ppb
This document describes simultaneous analyses of 7 fat soluble vitamins and derivatives by ESI negative mode. Excellent linearity (R2=0.99 or better) was obtained in each analysis.
5tablets↓
Add 25mL n-hexane and mix for 30 seconds then heat (60℃, 45min) ↓
Mix for 30 seconds,Centrifuge(3,000rpm, 10min) ↓
Filter supernatant (0.2um)
Sample Preparation 10x Sample Dilution
1000x Sample Dilution
10xSample Dilution
10x Sample Dilution
Vitamin D2 Vitamin EVitamin E
acetate Vitamin K1
20.75ppb(%RSD 8.60)
1191ppb(%RSD 2.04)
8913ppb(%RSD 1.46)
2379ppb(%RSD 4.48)
103.8 ng103.8 ng 11.9011.90μμgg 44.5744.57μμgg 5.9555.955μμgg
: Shim-pack XR-ODSⅡ (2.0 mmI.D. x 75 mmL., 2.2 μm): Methanol: Ethanol: 0 %B (0 -4min) - 50 %B (6-7 min) - 30 %B (7.01 - 10 min): 0.5 mL / min: 1μL: 40 ℃: 1.5 L / min: 10 L / min: 250℃: 400℃
Column Mobile phase AMobile phase BGradient programFlow rateInjection volumeColumn temperatureNebulizing gas flowDrying gas flowDL temperatureBH temperature
Table 2 Analytical Conditions
Vitamin A
0.0
2.5
(x1,000,000)
Vitamin D2
1.0
2.0
(x1,000,000)
0.0
Vitamin E
0.0
1.0
2.0
3.0
(x1,000,000)
Vitamin K1
0.0
2.5
5.0(x1,000,000)
Vitamin A palmitate
0.0 1.0 2.0 3.0 4.0 5.0 6.0 min
0.5
1.0
(x100,000)
0.0
Vitamin Eacetate
0.5
1.0
(x10,000,000)
0.0
Vitamin D3
1.0
2.0
(x1,000,000)
0.0
55--1000ppb1000ppb
0 250 500 750 Con.0.00
0.50
1.00
1.50Area(x100,000)
55--1000ppb1000ppb
0 250 500 750 Con.0.00
0.25
0.50
0.75
1.00
Area (x100,000)
Area (x100,000)
55--1000ppb1000ppb
0 250 500 750 Con.0.0
0.5
1.0
0 20000 40000 Con.
500500--50000ppb50000ppb
0.0
0.5
1.0Area (x10,000,000)
0 250 500 750 Con.
55--1000ppb1000ppb
0.0
2.0
4.0
6.0
8.0
Area (x100,000)
0 20000 40000 Con.0.0
2.0
4.0
6.0
Area (x100,000)
100100--50000ppb50000ppb
0 20000 40000 Con.0.0
1.0
2.0
3.0
Area (x100,000)
(x100)
1.25 1.50
0.0
2.0
4.0
6.0
8.0
397.40>69.15(+)
1.34
4
1.50 1.75
0.0
0.5
1.0
(x100,000)431.40>165.10(+)
1.67
9
2.25 2.46
0.0
0.5
1.0
1.5
(x1,000,000)473.40>207.05(+)
2.27
2
3.00 3.25
0.0
1.0
2.0
(x1,000)451.40>187.25(+)
3.06
8
