U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read...
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Transcript of U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read...
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U-PLEX®
Custom Biomarker (Human) Assays
Components
,
Quick Guide
Additional Materials
Reagent Preparation
Prepare Conjugated Capture and Detection Antibodies
Prepare the U-PLEX Plate
Step 1 Create the Individual U-PLEX-Coupled Antibody Solutions
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Step 2 Prepare the Multiplex Coating Solution
Step 3 Coat the U-PLEX Plate
Prepare Calibrator Standards
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Reagent Preparation
Reagent Preparation/Assay Protocol Dilute Samples
Prepare Detection Antibody Solution
Prepare 2X Read Buffer T
Step 1 Add Samples and Calibrators
Step 2 Wash and Add Detection Antibody Solution
Step 3 Wash and Read
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