I . flPOEAIOPIEMOZ ZHMEIOY flHEHt

AVTIKE~~EVO Kal m6io ~cpap~.loytj~

H 61a61~aoia a u ~ i ~a0opiCc1 TQ pi0060 avacpopag yla TOV npooB~opiapo TOU

aqp~iou nl j tqs a ~ a ~ i p y a m o u , n a a ~ ~ p ~ w p i v o u , UHT, ~ n ~ ~ ~ p y a a p i v o u K ~ I

~ ~ T o ~ T E ~ ~ w ~ ~ v o u T T ~ ~ ~ P O U S AUKTO TO<, ~&PIKWS ~ T T O P O U T U P W ~ ~ V O U V ~ A ~ K T O S

Kal ~ ~ T O ~ O U T U ~ W ~ ~ V O U ~ ~ A ~ K T o < , X ~ ~ U ~ ~ O ~ O ~ W V T ~ 6pyavo ( K~UOOK~TF IO

eEppiCJT0pa ) UTO 0ll0i0 TO ~ E ~ ~ O ( ~ T U T I K ~ ( E ~ E Y X O ~ E V O ~ O U T P ~ vfixETa1 PE

~ A E K T ~ I K O y l j ~ ~ q Kal i v a ~ ~ I E ~ E U V ~ T ~ ~ S 0~ppicr~opa a v ~ 1 ~ a 0 1 m a TO yuahlvo

0 ~ p p o p ~ ~ p 0 u6papyupou.

A I ~ T ~ ~ E V T ~ I 660 ~ l j r r o ~ opyavou . 0 ITPWTOS EIV~I iva 6pyavo nou a v ~ x v ~ l j ~ ~ TO

p fy lo~o oI')pEi0 lTf l<q~ OlqV ~Eploxf l O ~ I ~ O V T I O ~ T O ~ I ' ) D ~ S Tqs KaJJTrljh~s vlj<I')<, EVW 0 ~ E ~ T E P O S , yla E ~ T T O P I K O ~ < A~YOUS, ~ X E I P U ~ ~ I U T E ~ V U K ~ V E I pia avayvwaq

o~ iva ~a0oplapivo xpovo ~ E T U T ~ V ivapcq T ~ S ylj<q< . Ka0wq 01 K C I ~ T T ~ ~ A E S

oqphiou yljf,qs p n o p ~ i va 61acpipouv an6 TO i v a yaAa UTO aAAo Kal p&~a<l j

ydrhaKTo< Kal TWV T U ~ T O ~ T O ~ ~ J J ~ V U V 61aAu~~d~wv TTOU X P ~ U I ~ O T T O I O ~ V T ~ ~ yla Tq

paepovopqoq, q EV Aoyw pi006oq avacpopa~ a n a ~ ~ ~ i ~q xp j a q opyavwv nou

ava<q~o6v qv -rr~ploxfi op~<ov~~onoiqaq< . 'Opyava npo~a0op1opfvou xpovou

pnopo6v va ~pqo1porro1q0ouv yla ~ E T P ~ ( J E I < p o u ~ i v a ~

avixv~uoqq/61axwp1ayo6 .

Mla 60~lpaO~lKfi 66aq Y~AUKTO< U T I E ~ ~ J ~ ~ X E T ~ I (TTqV ~a~ahhqhq 8Epp0Kpa~ia,

avaAoya pE TO 6pyav0, Kal fl K ~ U ~ T U A A W D ~ 61&~&ip&Tal p& l ~qxav l~ f i 60vq~q

nou n p o ~ a h ~ i ~q ypfiyopq avo60 TV< O~ppo~pao ia~ twq pia nsp~oxrj

op~<ovr~onoi~aqq q o-rroia av~lcrro~x~i UTO aqp~io ~ ~ f i g q q TOU 6 ~ i y p a ~ o ~ .

To opyavo pa0povop~i~al npoaappoyfj TOU h o r ~ va 6ivm TQ owmi

avayvwaq yla 6IJo npo~una BlahQpa~a, xpqa~porro~wv~a~ qv i61a 61a61~auia

6 i~w< Kal yla 6E;y~a~a ydrha~To< . K ~ T W aTT6 TIS ( S U V ~ ~ ~ K E S ~ U T ~ S , TT€plO)(~

opr<ov~~onoirlorlq arrosi6~1 TO oqp~io nr\<qq TOU yaha~roq UE paepooq

K~haiou.

4 . Opyava ital yuah~va U K E U ~

To ~ p u o ~ ~ b r r r o a - r ro~ f i~ i~ar an6 bva ~ E ~ ~ O C F T ~ T I K U E A E Y X ~ ~ E V O Aourpo yIJ{qq,

iva B IE~EUV~TI~ B~ppio~opa ( bva B E P ~ O ~ E T ~ O av~io~aoq< qp~aywyoir ) PE

O U V ~ E ~ E ~ ~ V O K ~ K A w ~ ~ Kal ~ U ~ ~ U V O ~ E T ~ O 4 616~akll avayvwoqq, i v a

ava6~u~rjpa ~ E ~ ~ ~ u T O ~ Kal OUCJKEU~~ yla TqV E K K ~ v ~ C J ~ Tq< Tifi<q< pa<; PE

owh jv&g uno60x i~ TOU 6&iypaT0~ .

'Eva Kah6 ~ O V W ~ ~ V O uypb n O U lT&pl i~&l ~a~ahhqho ~ U K T I K ~ U Y P ~ , TO onoio

ava6&6&~al i ~ o l Ci)o~& q 61acpopa B ~ p p o ~ p a o i a ~ p&~a<O 600 TUXOVTWV oqpdwv

TOU uypoci va pqv urr~pPaiv~l TOU 0,2 OC. H B~ppo~paoia TOU uypoir ~ E V

n p i n ~ l va ~upaiv~rar ~ & p l ~ ~ 6 ~ & p O an6 f 0,5 OC an6 qv ovopuo~r~f i ~ ~ p j TTOU

6qhwv&~al an6 TOV K ~ T ~ ( J K E U ~ U T ~ ~ .

Eival oqpa\n~~o, TO uypo crro hou~po ytj(qc va 61a~r )p~ i~a t o~ m a 9 ~ p o

& T T ~ T T & ~ O . OAb~hqpq Q &TTl@~&la TOU owhjva ull060)(fi$ 6&iypaT0< K ~ T W ~ T T O

Tq ~apay f i CV~EI<QS ~ ~ K O U nptTf&l va ~ahhTT&Tal an6 TO ~ J U K T ~ K ~ U Y ~ O .

'Eva ouvexiq p&Opa ~a~ahhqhou ~JUKTIKOU uypoll KuKho(~op&i yljpu an6 TO

owhjva u n o 6 o ~ j ~ G~iypa~oq. H B~ppo~paoia TOU 6 ~ i y p a ~ o ~ 6 ~ v n p i r r ~ l va

K U ~ . J ~ ~ V & T ~ I T T E ~ I u C T ~ T E ~ O f 0,5 ' C ~ T T O TQV O V O ~ ~ U T I K ~ ~ Tlpj T O U 6qhCi)v&~a1

an6 TOV K ~ T ~ U K E U ~ U T ~ ~ .

'Eva KaTaMrlho ~ U K T I K O uypb &ival U ~ ~ T I K O 61ahupa 33% (vlv) 1,2

a10avo616hqg (a19uh~voyhu~6hq~ ).

0 @&ppio~opa< n p t n ~ l va EIV~I TOTTOU YUC~AIVOU ~ a e ~ ~ r j p a , 61api~pou 6x1

p&yahOr&pq~ an6 1,80 f 0,2 mm Kal 6lapi~pou OX^)$ 6x1 p&Tahl?T&pq~ an6

0,31 mm . H o~a8spa xpovou TOU B~ppbropa T T ~ ~ T T E I va ~ i v a ~ ~ I K ~ O T E P Q an6

660 ~ E U T E P ~ ~ E T T T U Kal Q Tlpi TOU P (PA~TTE (sQ~E~wDQ) T T P ~ ~ E I Va &ival uvqhi .

'OT~V 0 K U ~ E T ~ ) P ~ < E~VUI UTq Biorl ~ E ~ T O U P Y ~ U < OTO KPUOUK~TTIO, Q a ~ p q TqS

yuakvqq ocpaipaq, T ~ P E ~ E I va K E ~ T ~ I navu UTOV atova TOU uwhjva urro6oxrj<

6ciyparoq Kal U& iva aqp~ io 44,6 -+ 0,1 mm KUTW an6 qv ~oputptj TOU owhtiva

(@him oxrjpa U T ~ u~hi6a128). f l p i r r ~ ~ va urrdrpx~l iva urrocrrtjp~ypa yla va

por166 TO ~ & l ~ l U T f i Va BEUEI TOV K ~ B E T ~ ~ C ~ OTq @CUT) UIJTfi .

T civa~ r) e~ppo~pawia U& Kelvin,

R cival q avrio~aoq o& ohms as Ocppo~paoia V,

p ~ i v a ~ pia a ~ a e ~ p a rrou & < a p ~ a ~ a ~ arro TO UAIKO ~ a r a u ~ ~ u f i $ TOU f3cpl~ia~opa.

~ T T ] 0uvfieq TTpClKTlKfi U U V ~ U T ~ T ~ I FlCl ~ 1 ~ 0 TTOU ~ E T T E P V ~ ( TO 3 000 .

H av~ io~auq TOU O~ppiu~opa .rrpina va a v ~ r a r a Q ~ ~ o ~ & i VE ~q PofiB~~a

yicpupac Wheatsdtone fi nap6porag 61~Ta<q$, ~pqo rpono rdv~a~ ara€J&pouq

pueprcr~iq av~imaaqc uyr lht j~ rrororrl~a<, TUV o.rroiwv rl avoxfi 6 ~ v ~ i v a ~

pEyUh6T~p~ T ~ U i 10% Kal TwV onoiwv 0 OUVT&A&UT~~< 0&ppo~paoia( ~ E V

urr~pf3aiv~l TO 2 X 10 '~ C.

H U T U ~ & ~ O T ~ T U TqS 616Ta{q< ~.liTpqUq~ Kal TOU UVT~UTO~XOU KUK~WC(UTO<

~ ~ E T T E I va ~ i v a l ~ i ~ o l a rrou 6 1 a 6 0 ~ 1 ~ i q E V ~ E ~ ~ E I ~ T ~ S i61aq B~ppo~paoiaq va

pqv ixouv p&yahirT&prl 61arpopa an6 0,001 "C.

H Y P ~ ~ ~ I K ~ T ~ T ~ TOU K U K ~ W ~ ~ T O ~ TTP~TTEI Va €ival TiTOla WUTE Va lJqV

~ l a a y ~ ~ a l orpahpa p ~ y a h l i ~ ~ p o an6 + 0,00I0C a& ~ a p i a n ~ p l o x j p&~a{ir

-0,400 "C Kal-0,600 "C O T ~ V To 6pyavo A E I T O U ~ Y E ~ U W O T ~ .

'Eva oirppa an6 pi~ahho a6paviq oTo yaha Kal p& ~ I U ~ E T ~ O p&Ta{6 1 Kal 1,s

mm ~pqolpoi-rol~i~al yla Tqv ava6~uoq Tqq 6 0 ~ l p a ~ T l ~ f i ~ 66oqq.

To oirppa a v a 6 ~ u u q ~ n p i r r ~ l va i-rpooapp6<&~al wq npoq TO T r h 6 ~ 0 ~ TOU Kal

Va ToTTo@€TE~T~I K ~ @ E T ~ p& TO &lTi77&60 TOU K~TWTEPOU UKPOU TOU i610 p& TO

a ~ p o sou ~ a e ~ ~ j p a TOU B~ppimopa . ETTIT~ET~ETU~ pla avoxtj TTE~~TTOU I ,5 mm

T T ~ v w fi K ~ T W al l6 Tq @foQ ~ u T Q .

To oirppa ava6~uoqq i - r p i n ~ ~ va BOVE~T~I E T T I ~ ~ K W S PE Eva ~ n a p ~ i c lThaT0c

~ ~ A O I ~ ~ E V O aTT6 TOV K C ~ T C ~ U K E U ~ U T ~ (0x1 ~ I K P ~ T E P O an6 f 1,5 mm) yla Va

4.1.5 . AlaTa{q npoq ivapcq T ~ S q~ii{qq

A U T ~ pnop~ i va ~ i v a l O T T O I ~ ~ I ~ T T O T E 6ldTa{q q oi-roia ~ a ~ a ~q h~lroupyia T ~ S

r r p o ~ a h ~ i ~ t - l o~lyplaia q~ireq TOU B~iypa~oq i ~ a t WUTE q B~ppo~paoia T ~ G

~ O K I ~ ~ U T I K ~ ~ 66oqq a v i p x ~ ~ a l UTO oqp~io ni{qq. Mrrop~i va ~pqcs~pono~q@~i

yla TO o ~ o n 6 a u ~ 6 TO o6ppa ava6~uoqq - pia pi0060q ~ i va l va au£,rl€Jei TO

T'rhdrT0~ 66vqorlq &ni iva iwq 600 ~ E U T E ~ ~ ~ E T T T ~ ~ T D I L~(JTE TO CJI.jppa

ava6~uaqq va r r poa~po l j o~~ o ~ o ~oixwpa sou owh jva un06o~fiq 6~ i ypa~oq .

01 owhjv&< uw06o~fl< 6 ~ i y p a ~ o q (ph iw~ oxflpa o ~ q o ~ h i 6 a 128) w p i r r ~ ~ va

~ i v a l ~ a ~ a o ~ ~ u a o p i v o l a n d yuahi Kal va &ouv p f i ~ o ~ 50,8 + 0, l mm,

E~,WT&PIK~ ~ I ~ ~ E T P O 16,O 2 0,1 mm Kal EUWTEP~K~) ~ I U ~ E T P O 13,5 + 0,1 mm . TO

w~)(o< TOU T O ~ X W ~ U T O S D& 6ho TO pflK0< TOU owhjva ~ E V T T ~ ~ T T E I Va

pETapdrhh&Tal TTE~IUUOTEPO and 0, l mm.

01 o w h j v ~ < TTP~WEI va ( ~ i p o u v xapayfi iv6&1(q< 6 y ~ o u 29,8 mm KUTW an6 TO

a ~ p o (21 mm navw aTI.6 ~q paaq TOU owhjva) w p o ~ iv6&1Cq, av~ io~o lxa ,

6 y ~ o u 6&iypaT0< 2,5 5 0,l mm.

H ~ E K T ~ I K ~ S (~oOpvo<, Kahdr E~,UE~I<O~EVO~, I K ~ V O ~ va 6 1 a ~ r l p ~ i B ~ p p o ~ p a r ~ i a q

300 f 25 "C.

5. A ~ ~ l d p a ~ ~ ~ j p l a

5.1 . N ~ p 6 a n ~ o ~ a y p i v o UE P O ~ O ~ U P I T I K ~ yuahi, ppaaeiv Kal q~uxeiv o~ous 20 3 2

"C UE cp~ahq ~cpo61aapivq p& owh jva arroppocpqoq< 610~~16/0u TOU avepa~a.

Zuyi<&Tal Q ~a~ahhqhq T T O U O T ~ T ~ @f i l l& TTivaKa KUTWT@W) f,qpOC X A W ~ I O ~ X O U

va~piou (PhinE crqp~io 5.2) UE cp~drhq <~\ylaq< . Alah l j~~al UE a-rr~maypivo v ~ p 6

( ~ A ~ T E oqp~ io 5.1), ynacp ip~~a~ TTOOO-~KU UE o y ~ o p ~ ~ p M yrahv pias xapayqg

TWV 1 000 ml Kal a p a ~ d v ~ ~ a ~ p& v ~ p o p ixp~ ~q xapayli mou< 20 + 2 "C.

nplv xpqcr1pono1q9~i i va n p o ~ u n o 61ahupa, q cp~ahq a v a a ~ p i c p ~ ~ a ~ ~hacppa Kal

TT~plUTpicp~Tal U ~ K E T ~ < cpopic yla va a v a p ~ 1 ~ 8 ~ i ~ a h a TO T T E ~ I E X ~ ~ E V ~ ~ q < . ZE

~ a p 1 6 n~p i r r~wcrq 6&v np i r r c~ va ava~apax8d ~ 6 o o ~oxupa WUTE va

6qp1oupyqQoirv cpuoahi6~q a i p o ~ .

AEiypaTa npb~unwv 61ahupa~wv 8a acparpoOv~al ano TIC cp16lh&< 61a T ~ S

anoxuuq< ano ~q cp~ahq TOU 61ahOpa~oq, U& ~aBapo, <qp6, ~ u p i r o ~ o p o n o ~ f i p ~

JEW<, TTX al(~Li)vla ~ E V ~TP~TTEI Va X ~ ~ U ~ ~ O ~ T O ~ O ~ V T ~ I ROT^ yla TO OKOTTO ~ U T O .

LhahdpaTa 6 ~ v np in&l va xpqarpono~oirv~a~ ano cplah&< 01 onokq 6&v &ival

n h f i p ~ < KaTa TO bva T~TUPTO TOUS Kal EaV ~ E V UUVTQPO~VTUI p& ~ U K ~ T O K T O V O

( y~a napa6~1ypa 61ahupa thiomersal, 10grtl) Kal 6&v 8a ~pqo1pono106vra1 &av

~ i v a ~ -rrahalo~~pa TUV 6d0 pqvwv.

6. BafJpovopqaq TOU K ~ U O U K O ~ ~ O U 8&ppkJTopa

TO K~UOUKOTT~O TTP~TTEI Va E ~ K ~ T C ~ U T C ~ ~ E ~ bTUl ~ U T E ~~pp0KpaUiC.l TOU

-rr&plpahAov~a aEpa va prlv UTTOKA~VEI T ~ E ~ I U U O T E ~ O an0 l "C an6 ~q

8~ppoKpaCJia UTqV 0 ~ 0 i a lTpaypaT0TT0lfi8qK~ Q p a 8 ~ 0 ~ 6 j J ~ U ~ . TO K~uoUK~TT~O

~ E V TT~f l l~ l ~ a ' E K T ~ ~ E T ~ I U& qhlaK0 VWS l) p&fipaTCI Kal 8&ppoKpaUia 6wpaTioU

p&yah6T&pr) an6 26-27 'C .

E<aocpahi<&Tal OTI TO K ~ U O C J K ~ T ~ ~ O &ha1 U& Kahfi K ~ T U ~ T ~ U ~ ~ h~lT0Upyia~

~6pcpwva p& TIC o6qyi&g TOU K C ~ T ~ D K E U ~ U T ~ Kal OTI CXEI T E ~ E ~ UE AE~TOUPY~Q

rouhd~ru~ov 12 6 p ~ g nprv ~q paepovbpqoq . EMyx&~al q BEoq TOU ~aO&~f ipa,

TO TThdT0< 66vqaq~ TOU oOppaT02 ava6~uoq< Kal q B~ppo~pacsia TWV

~ J U K T I K ~ V uyphv.

EnlhEyovTal 660 n p o ~ u n a 61ah6pa~a (phiTrE n i v a ~ a a v w ~ i p w ) nou

TT&PIK~E~OUV K ~ T U npooiyy~uq TVV ~ V ~ ~ E V O ~ E V ~ Tlpl) TOU (SI]C]E~OU T T ~ ~ < T ) S TWV

~ E I Y I J ~ T W V YU~UKTOS TPOS avahuoq . H 61acpopa p&Ta<lj Twv D ~ ~ E ~ w v nfi<q<

Twv 660 61aAupa~wv l l p i l l ~ l , KUTU T T ~ O T / ~ T ) D T J , Va pqv ~ i v a l ~ I K ~ O T E ~ ~ an6

-0,100 "C.

(E& OplUpCVa povrfha K~UOUKOTT~WV 61aBfa1pwv KUTU TQV n~p io60 aUTfi, TO

o u v 6 ~ 6 ~ p i v o PE TO B~pp iu~opa KljKhwpa ~ X E I o x ~ 6 1 a m ~ i WUTE va

~ { l a o p p o n ~ i ~ a ~ UE pia ~161Kfi ~ l p t j TOU uqp~iou nfi{q< EVTOC ~ q < T T E ~ I O X ~ ~ S

piTpqUq< TOU OpyaVOU . E& alJTf< TIS T T E P I T T T ~ ~ E I < Q xpfioq &V6< TTP~TUTTOU

61ahljpa~o< f x o v ~ o ~ a u ~ o To uqp~ io ~ ~ t j { q q uav fva an6 Ta 61ah6pa~a

paBpovopqoq~, ~ I E U K O ~ U V E I Tq 61a61~aoia pa8povopquq~ Kal 0

K ~ T C ~ U K & U ~ U T ~ ~ < TTPCTTEI VU U T T O ~ E I K V ~ E I TqV T l ~ f i U U T ~ ~ ) .

M ~ ~ a c p i p o v ~ a ~ pc ulcpwv~o 2,5 * O, I ml EVOS T T ~ ~ T U T T O U pfoa U& ~aBap6,

O T E ~ V O owhfiva U ITO~OX~~S 6EiypaT0< Kal TO K~UOOKOTTIO Ti8ETal U& h&lToupyia.

H /3aepovopr~aq &hiy~&Tal pE TTp05appoytj, OVW< U ~ ~ O ~ E I K V ~ E T U ~ aTr6 TOV

K ~ T ~ U K E U ~ U T ~ ~ , Ew< OTOU ~ V ~ E I < T ) avdyvwoq~ TOU K ~ U O ~ K O T T ~ O U EiVal iUq p€

TO o q p ~ i o n t j < q ~ TOU ~ ~ O T U T T O U 61ahtlpaTo< . H 61~6l~aoiU ~r ravahappav~~ar

p& To ahho ~ ~ O T U ~ O 61ahupa Kal UUV&X~<ETU~ EVU~~UKTIKU p& TOV i610 ~ ~ 6 x 0 ,

CW< OTOU 61a60~1~6< avayvLbo&l< K ~ O E 61ahljpa~o<, x ~ p i < TT&palThpW

-rrpooappoyfi ~h i yxou T ~ S paQpovopquq~, 6ivouv qv a w o ~ f i rlpfi TOU oqp~iou

Trfl<rl< KU~EVOF . TO K~UOUKO~TIO EiVal TOTE fT0lp0 TTPOS x p f i ~ ~ r l KUl Ba 6&i{&1

K ~ T E U ~ E ~ U V TO u q p ~ i o TTO<QS TOU 6EiypClTO~ ydrhClKTO<, xwpi? TqV &~appoyf i

onolau6rj-T~OTE 616pewuq<.

7 . flpor~orpaaia TOU 6&iypaT0~ npoq avahuuq

7.2. Anopa~p6vov~al an6 TO Bdypa TUXOV {Eva a d p a ~ a rj ar&p&6 Poir~upo, E ~ V

x p ~ l a w ~ i , PE 61fieqoq pioa a& ~a0ap6, ~ T E Y V O 60xti0, Kal TO 6 ~ i y p a

a v a p ~ ~ y v u ~ ~ a ~ ~hacppa . 0 qBp65, ~ a v xpqo~pono~qBd, n p i r r ~ ~ va ~ i v a ~

a6pavtj~ o ~ o yaha Kal va p n o p ~ i va xpqatpo~orqB~i a& B ~ p p o ~ p a a i a

~pyaorqpiou .

7.3. To yaha p n o p ~ i va avahueci ~ T U V ~ i v a l o ~ q B ~ p p o ~ p a o i a 6la~tjprlaq$ TOU (

&Ta<~~1/6 0 Kal 5 'C ) tj p n o p ~ i VCI acp~Bci va CITTOKT~OEI ~q B~ppo~pao ia

~pyacsrqpiou ap iaws nplv an6 qv ivap<q TV< avahuoq< . C!UTOUO, ~ i v a ~

arrcrpai~q~o Ta nporuna 61aADpa~a Kal Ta B&iypa~a y M a ~ ~ o < va ~ivar orqv i61a

B~ppo~paoia o ~ a v ~pqu~porroroOv~a~ .

7.4. H ~lTh060T~Dlptl 0<6TqTa TOU yahaKTO< lTpOU6lOpi<&T~l, KaTdr TO ~UVUTOV,

~au~oxpova PE qv avahuuq TOU oqp~iou rrQ<qq . A~iypara PE o < l j ~ q ~ a

un~pPaivouoa r a 0,18 g yahaKTlK06 o{Co< av6 100 ml y d h a ~ s o ~ 6 ~ v pnopoOv

Va & { & T ~ U T O ~ V .

7.5. To UHT, TO & n ~ < ~ p y a a p i v o Kal TO a r r o o ~ ~ ~ p w p i v o yaha n p t n ~ ~ va p i va ~ r r i

T O U ~ ~ X I U T O V 20 ~ E T T T U a& ~ V O I K T ~ 60x~io nplV &<ETu(~TE~.

Ehiyx&Tal ~ a v TO E~ri~fE60 Kal q B~ppo~paaia TOU WUKT~KOI~ uyp06 ~ i v a ~ UE

CTU~cpwvia p& TIC 06qyiq TOU K~T~UKEUCIUT~~ Kal E ~ V , E ~ ~ O U O V X~EIUUTE~, 0

~ a B ~ ~ r j p a $ TOU e ~ p p i o ~ o p a ~ i v a ~ pioa a& Eva U ~ E I O owhrjva un06o~f i<

6&iypaTO< UTqV K O ~ ~ O T T J T ~ U T T O ~ O X ~ ~ < 6 ~ i y p a r o ~ . TO K ~ U O ~ K O ~ I O T ~ ~ E T U I D E

h&lT~Upyia Kal ~caocpah i<~~a~ OTI TO ~JUKTIKO uypo ava6&B&~a1 fi K U K A O ~ ~ O ~ E ~

uwarb, b r r w ~ &-rrr~ahh&~ar . Aqou TO ~puoo~o-rrro ~ i va r a& h~t~oupy ia &TT;

~ o u h a ~ ~ o ~ o v 12 &p&<, E ~ ~ Y X E T ~ I q B~ppo~paaia TOU ~JUKTIKOO uypou KCI q 8 ioq

Kal TO TfhaT0~ a v a 6 ~ u o q ~ TOU o6pparo~ ava6~uoqq.

nplv an0 K@& K ~ K ~ O ~Aiyxou, ~ E T P ~ T U I TO U T ) ~ & ~ O TTif,TJ< TTP~TUTTOU

Blahirpa~o< ~hwpro i r~ou va~piou ( n . ~ . 61ahupa p& oqp~io nfi€,q< -0,512 ' C )

Cwg OTOU diro 61a60~1~o i npoa61opropoi 6 ~ v 61acpipouv K ~ T U r r ~ p r ( ~ a 6 r ~ p o ano

0,001 "C . Eav o pioog opog TWV T I ~ L ~ V a u ~ d v 61acpip~1 arro TO a q p ~ i o rrlif,q<

TOU n p 6 ~ u n o u 61ahBpa~os r r ~ p ~ o a o ~ ~ p o an6 0,002 "C, TO K~UOCTKOTTIO

j3aQpovop~i~al {ava onwc n & p ~ y p d ( ~ q ~ & (TTO aqpcio 6.

Eav TO ~puocs~brrlo X ~ ~ U I ~ O ~ O I E ~ T ~ I CTUVEX~<, o ih&yxo< pa9povbprluqc;

p o u ~ i v a ~ E K T E A E ~ T ~ I T O U ~ ~ X I ~ T O V pia cpopa ava d p a . n p i r r ~ ~ va Aappttvov~a~

urroyq 01 06qyi~s TOU ~ a ~ a a ~ ~ u a r n t j .

H cp16Aq p& TO 6Eiyj.J~ TOU yahaKTO< Cl~CYOlpicp&Tal ~Aacppa Kal T T & P I C T T ~ ~ ( P & T ~ ~

U ~ K E T ~ ~ cpopb~ yla va avap~1~9oirv Ta T T E ~ I E X ~ ~ E V ~ T T ) ~ . ~ a p i a ( T T I Y ~ ~ ~ E V

T T ~ ~ T T E I i v a 6&iypa va ava~apax8~ i TODO Swqpd nou va &y~h~plO'T&i a i p a ~ .

MET~(P~POVT~I C]& UI(PL~)VIO 2,5 k 0,l I l l 1 TOU V ~ ~ U K T O < pi(Ta D& ~aeap6 , OTEYVO

owh j v a uno6o~fi< 6&iyparo< Kal anopa~pl \v&~ar TUXOV rrA~6vaapa p&

orcphv~o. E<aocpahi<&~al 6 ~ 1 o ~ a 9 ~ ~ t j p a s Kal TO oirppa ava6&uaq< ~ i v a ~

~a9apa Kal UTE~VU, a~ouni<ovrag Ta, ~ a v X ~ E I ~ O T E ~ , PE i va paha~o, ~aQap6,

xwpis xvou61 cjqaapa ano KUTW rrpoq Ta n d w .

0 awhrjvar; u r r o 6 o ~ t j ~ 6&iypaTO< &loay&Tal o ~ o pa9povopqpivo ~ p u o u ~ o m o

06pcpwvCl PE TIC 06q~iE< TOU KC~TU(~KEUC~(ST~~ . TO yaha &l lyUx@&i KUl TJ l lr jfrl8a

a p x i u ~ ~ U& pra B~ppo~paa ia rrou ~ a e o p i < ~ ~ a r an6 TOV ~ a ~ a o ~ ~ u a c r r r j p&

TTpoaiyy1(Tq 0,l '=C.

(IE op~apiva a u ~ o p a ~ a opyava rl B ~ p p o ~ p a o i a a u ~ r j prropci va rrapa~qpq9ci

o~ ~ q c p ~ a ~ t j 616r~aSq avayvwaqg - a& x ~ l p o ~ i v q ~ a opyava q ar rapa i~q~q

Eav, yra O T T O ~ O ~ I ~ ~ O T E h6y0, q nfi{q apxio~r nplv fi ~ E T U T ~ V ~a00prapivrl

~ E P I O X ~ ~ @&pp~Kpaaia~, rl 60~1pt) &y~~~Clh&in&fal Kal &TTa~ahappdrv&Tal p& ahhrl

60Klpa~TlKfi 6 6 ~ ~ ToU ~ M ~ K T o ~ . Eav Kal To &TravahtlTrTlKo 6~iypa TTI~<EI nplv

qv ~aeoplopfvq B~ppo~paaia, pia n ~ p a ~ ~ f p w 66oq TOU G ~ i y p a ~ o ~ r r p i r r ~ ~ va

B~ppav0~ i crrouq 45 T Car va 61a~qpqO~i ~ n i ~ ~ V T E h ~ n ~ a yla va pi-ropfa~r va

A I ~ U E I TO ~p~ffTahhlK6 hinoq.

METU yirx&Tal {ava o ~ q B~ppo~paaia avahuoqq Kai avahl j~~ar apiowq. H

B~ppo~paoia TOU y a h a ~ ~ o ~ p e ~ a qv tvap<q ~ q q rr+?,qq @a a v i p ~ ~ yptjyopa UE

pra ~ l p t j q onoia 0a n a p a p ~ i v ~ ~ ouo~aorr~a o~aB~p t j &rr.i ~ a n o ~ o XPOVIK~

6ld~Tqpa nptv T'rio&l Kal nahl. TO D T ] ~ & ~ o n f i < q ~ a v r l o ~ o l ~ ~ i OTqV uyqAo~&pq

e&ppOKpaUia TTOU ~ R O K T ~ ~ ~ Q K E K ~ T U TqV n~pj060 UUT~ , Kal auT4 Q Tlpfi TTP~TTEI

va ~a~aypacp~i.

To xpovi~o 61aa~qva ~ a ~ a TO o-rroio q B~ppo~paoia napapiv~r o ~ a e ~ p l j Kal TO

xpovt~o 6160Tqpa HET^<^ T Q ~ ivaptqq TT\< n q t q ~ Kal T ~ S E ~ ~ T E U < ~ S T ~ S

uyqho~~pq< e~ppo~paoiaq 0a 61acpipe1 an6 6~iypa a& 6~ iypa Kal Qa ~ i v a ~

o q p a v ~ ~ ~ a ~ I K ~ O T E ~ O yra TO v ~ p o Kal Ta npo~una Blahirpa~a ~ h w p ~ o t i ~ o u

va~piou an6 671 yra TO yaha. Eiva~ anapai~q~o, q uyqh6~~pr l Q~ppo~paoia va

~ i v a ~ a u ~ l i q o ~ ~ o i a ~ a ~ a y p a c p ~ ~ a ~ .

' O T ~ V Q phpqaq EXEI o ~ o K ~ Q ~ w ~ E ~ IK~VOTTO~~TIKU, 0 ~uh f i vaq aTT006p&T~l,

& K T T ~ ~ & T ~ I p& VEPO Kal 0 KCIe~Tflpaq TOU @&ppiCJTOpa Kal 0 ava6EuTtjpaq

a~&yvchvov~a~ p& paha~o, Kaeap6, xwpiq xvo661 6rpaopa an6 K ~ T W TTPOG Ta

navw Kal E K T E ~ E ~ T ~ ~ T T ~ V O ~ O ~ O T U T I O ~ T T ~ O U ~ I O ~ I C J ~ O ~ 5 E aAhq 6 6 0 ~ TOU

6€iyl.laTO< ~ u ~ ~ K T o < .

Eav r l 61acpopa Twv aqp&iwv Trfi<q< TTOU a n o ~ ~ f i B q ~ a v Eival p&yah6~&pq an6

TqV 11pfi E ~ T C ~ V ~ ~ ~ W ~ ~ O T ~ T ~ ~ (0,004 "C), &~T&h&iTCll T T ~ V O ~ O ~ O T U T T O ~

rrpoo61op1apo< a& ahhq 60oq TOU B&iypa~o< . Eq6oov 01 600 ~~poa61op1opoi

oupcpwvo0v p ioa a& 6pra TWV 0,004 "C, 01 ~ r p i < r r p i n ~ ~ va ~a~aypacpodv Kal

VU X P ~ U I ~ O T T O ~ ~ ~ O ~ V UTOV UTTOh0yl~pb TOU T E ~ I K O ~ ~ T T o T E ~ ~ u ~ ~ T o ~

8.4 . v6<q TOU Ka@&Tfipa

Eav, a~ohou86v~a< TOV i h ~ y x o Pa8povbpqoq< pou~iva<, E T T I P E P ~ I W ~ E ~ q

paBpovopqoq, uTTohoyi<&~al 0 pf00< 0p0< TWV ~ T T O ~ E K T L ~ ) V ETTUV~~QTTTIKWV

T I ~ W V U ~ ~ E ~ O U nfi<Q< n O U nap&hficp0qoav, U T ~ O ~ ~ U ~ & ~ O V T U < OTqV TpiTrl

~ E K ~ ~ I K O 0 i ~ r l .

Eav TO drep0lUpa 660 ~ ~ T O ~ E K T W V E T T ~ V ~ ~ ~ T T T I K C ; ) V T I ~ ~ V dval TTEPITT~<

aprepoq, o pioos opo< rrpimr va ~TpOyy~hOTT01~8&i o ~ q v T i h ~ ~ l i U T & p ~ a p ~ ~ a

~ l p f i OTTWC ~ E ~ X V E I TO ~ K O ~ O U ~ O napa6~1ypa:

9.2 . A ~ p i b ~ l a

9.2.1 Enavahrlyqpo~q~a (r): 0,004 "C.

9.2.2. Avanapaywyi~o~q~a (R): 0,006 "C.

ZXHMA 1

h p & arbc Tube 1 & b d l o a

~ C P P ~ aud6cucr~~ S& & g j u m u

Kccpahr) %cpplmopo T W t o r bud .Biic. U . r u n dc d l a m i x x

2.1. H & V & ~ Y ~ T T ) T U cpwUcpCtTdrCJll< &iVal Eva php0 TqC T o C T ~ T I ~ T ~ < TIl< &V&pyoG

CIhKahlKtj~ cpwacpa~auqq UTO rrpo'ibv, ~~cppaupivq wq q I T O U ~ T ~ ) T ~ cpalvohqq, UE

pg ava rnl, nou ~ ~ Q ~ E U ~ E O E T ~ I K ~ T W an6 TIC ~ V ~ ~ O V E U ~ ~ E V E < OTT) 61a6r~aoia

U U V ~ ~ ~ K E S TOU ~ ~ U T E ~ I W ~ ~ V O U y6haKT0~.

2.2. Y a 8 ~ yaha TOU orroiou q E V E ~ Y O T ~ T ~ cpwacpa~auqq ~ i v a l K ~ T W an6 4 pglml

cpalvohrlq 0 ~ w p ~ i ~ a 1 apvq i l~o U& avTi6paUq cpwucpa~aoq$ .

H E V E ~ Y O T ~ T ~ cpwucpa~auqq a < ~ o h o y ~ i ~ a ~ air6 qv r r o a o ~ q ~ a cpalvohqq rrou

aTT06&Up&~&Tal an0 TO tpa~vuhocpwocpopl~b ~ I V ~ T ~ I O TfOU Trpouri0~~al DTO

6dypa . H a~~o6&up&upivq cparvbhq av~16pa p& 61~pwpo~1vovo~hwpoi:pi61o

rrapayov~aq 61ppwpo~iv6oa1v6Aq ( urro~ljavou xpwpa~oq) q o-rroia ~ E T P ~ T ~ I

X ~ W ~ O ~ E T ~ I K ~ crra 610 nm. r i v ~ ~ a ~ ul jy~plaq p& B~iypa UTO orroio TO iv<upo

T ~ S cpwocpa~aoqq ~ X E I ~ a ~ a u ~ p a c p ~ i .

4467

4.1.1. Alahljov~al 50,O g u6pof,~16iou paplou [Ba(OH)2-8H20] a& vepd Kal o 6 ~ ~ 0 5

U U ~ I T A ~ ~ W V E T ~ I fwq Ta 1 000 rnl.

4.1.2. Alahrjov~al 22,0 g pop1~06 0f,f05 (H3BO3) D& V E ~ O Kal 0 6 ~ ~ 0 5 U U ~ I T ~ ~ P W V E T ~ I

i w ~ Ta 1 000 rnl.

4.1.3. O ~ p p a i v o v ~ a ~ 500 ml K U ~ E BlahljpaTo< TOU US 50 'C , TQ 61ahtjpa~a

avap~~yvOov~a~, ava6erjov~a1, y6xov~u1 yptjyopa o ~ o u ~ 20 "C, TO pH

61opewv~sa1, Eav ~pElacr~EI', DTa 10,6 f 0,1 p& T T ~ O O ~ ~ ~ K T J 6raArjpa~o[; 4.1.1 0 4.1.2 . A~tjeqaq . To 616hupa iSla~qp&i~a~ a& o~&yava n w p a ~ ~ o p i v o 60x~io.

Alah6ov~al 6,O g p ~ ~ a P o p l ~ 0 6 va~piou (NaB02) tj 12,6 g (NaBO2, 4H20,) Kal

20,O g xhwp~o~;xou va~piou (NaCI) o~ v ~ p o Kal o o y ~ o ~ a u p n h q p ~ v ~ ~ a ~ f w ~ Ta

1 000 ml.

Alahliov~al 0 , l g avu6pou c p ~ l ~ ~ h ~ c p ~ U c p ~ p l ~ ~ l \ 61va~plou anqhhaypfvou

cpalvohrl~ UE 100 m1 ~ U ~ ~ I U T I K O ~ 6 l~h6pa~0< (4.1.3) tj 6lah60v~al 0,5 m1

cpa1vuhocpwacpopl~o6 ~ I V ~ T ~ I O U UE 4,5 ml ~ U ~ ~ I C J T I K O ~ 6 lah l jpa~o~ a v a n ~ u < q ~

X ~ W ~ ~ T O < (4.2), T ~ o U T ~ ~ E V T ~ ~ 660 o-fayovE~ 6lahirpa~oq BQC (4.6) Kal

acp j v ~ i a ~ (SE Beppo~pauia ~ W ~ U T ~ O U ~ n i 30 ~ETTTU. TO xp6pa nou o x q p a ~ y ~ ~ a ~

fTU1 & K X U ~ ~ < E T ~ I PE 2,5 m1 pou~av-I-dhrl$- Kal ClcpfivETal US r l ~OUTUV-1-0hq

61axwpla~~i. H pourav-l-OAT) acpa~pd~a~ Kal a-rroppin~c~a~. Eav xpe~au~ei, T)

~~x l j h l oq enavahappav~~a~ .

Alahljov~al 3,O g OEIIKO~ tpeu6apyljpou ( ZnS04, 7H20 ) Kal 0,6 g OEIIKO~~

xahKo6 (11) (CuS04, 5H20) UE vep6 Kal o o y ~ o ~ auprrAqp6v~~ar i w ~ oTa 100

ml.

4.6. A16hupa 2,6-61Ppwpo~lvovo~hwp1pi61ou (61aAupa BQC)

Alahljov~al 40 -1: 1 mg 2,6-61ppwp1~ovovo~hwp1pi61ou (BQC) (C6H2BrzCIN06)

LSE 10 ml a10av6hq~ 96% (vlv).

A la~r lp~ i~a l UE (p~ahq aKoOpou xpwpa~o5 aE yuy~io. Eav ~ i v a l

a r ro~pwpa~ la~ ivo ri nah16~epo an6 tva gova arroppirn~~al .

Alahljov~al 0,05 g 0 ~ l l ~ o l j x~hK06 (11) (CuS04, 5H20) a& vepb Kal o o y ~ o ~

O~pTrhIlpb~ETal i w ~ ~a 100 ml.

4.7. npb-ru-rra 6lahOpa~a rpalvohqq

4.8.1. Zuyi<ov~ar 200 -+ 2 rng ~aeaprjq avu6pqs cpalvohqs, jJ~~acpfpov~ar DE

O ~ K O ~ E T ~ I K T ~ cp~ahq TWV 100 ml, npomi0e~a1 vepo, avape1yv6~~a1 Kal o o y ~ o ~

oupl~Aqp&ve~ar twq ~q xapayrj. AUTO TO p q ~ p r ~ o 6rahupa n a p a p i v ~ ~ a ~ a e ~ p 6

&TT[ ~ P K & T O ~ < !qv&< CJTO ~+JuYE~o.

5. 'Opyava Kar yuakva o ~ ~ l i q

(a) 6Aa Ta yuahrva IJK&~Q, ?a n b p a ~ a Kar Ta ~pyahda 6~1yya~oAr lq~ ia~

npi-rr~r va ~ivar T T ~ O ~ E K T I K ~ ~a0apropiva. tuv~o-ra~ar va EKTTAUBOISV FE

mp6ocpa.r~ ppacrpfvo a~~~maypCvo v ~ p 6 rj va a~pro~ol jv - (p) opropfvo~ T~TTOI TThalsrlKcirv T T W ~ ~ T W V ~ ~ T O ~ O U V Va ~ T / ~ O K ~ ~ ~ D O V V

p6Auvoq NE (parvohl~i~ oucri~< Kal q xpjoq TOUS ~ E V T F ~ ~ T T E I va

ETTITP~TTET~I.

5.3. ~aapa~ocpwroye~po KaT6Ahqho yra avayvdo~ r~ o& y r j~os K6paTO< 61 0 nm .

5.4. AOKI/.JUUTIKO~ awArjv&<, 16 t) 18 mm X 150 mm, ~ a ~ a TTpoTipr)~~

paeyohoyq y fvor crTa 5 Kar 10 ml .

5.5. trcp6vra

5.6. ruah~va xwvrd, ~a~ahhqhou y ~ y i 8 o u ~ , yla ~rapa6~1ypa 6lapiTpOu 5 cm.

(U) aTT0~~6yETal q ~ni6paoq aTf&U8&ia< qhlCl~06 9 ~ ~ 6 5 KaT6 Tq 616pKEla

TOW TTPOU~IOPIU~O~~ - (p) p6Auvuq p& ixvq uah~ou lj avanvorj< pnopd va 6doa ~ocpahpfva

~ E T I K U U T ~ O T E ~ ~ U ~ ~ T ~ Kal TTP~T~EI Va alTO~E6yETal. An0 TqV drlT0lyq aUTq,

16laiTEpQ ~ T ~ o u o x ~ ~ TTpflTEl Va ~ ~ V E T ~ I UTQ 6la61~aoia U I ~ ~ W V I U ~ O ~ .

6.1. n p o ~ ~ o ~ p a u i a TOU 6&iypaTO< npo< avahuoq

6.1 . l . H avahuoq ITpaypa~OTrOld~al a p f o w ~ p&Tdr TQ 6ElypaTOhIllyia . A~C~(POPETIK~~, TO

6~iypa cpuMaouE~al U& lyuydo, aMa 6x1 yla T T E ~ I U U ~ T E ~ O an6 6130 qpfp~<.

METacpfpETal p& UI(PL;)VIO pfoa UE ~ a e i v a an6 660 ~ O K I ~ ~ C J T I K O ~ ~ owhjv&<

(5.4) 1 ml TOW 6~iypa~oq n p o ~ avahuoq, ~ p q u l p o n o l d v ~ a ~ fva uwhljva W<

ivav i h ~ y x o rj pap~upa (~ucpho).

6.3.1. 0 pap~upac B&ppaiv&Tal &lTi 660 h ~ m a U& < ~ O V V E ~ O - 0 ~ O K ~ ~ ~ U T I K ~ <

owhfiva< Kal TO no~l jp l <tor)< v~pol j Kah67TTO~Tal pc cpchho ahoupiv~ou yla va

~f~Ucpahl~T&i q Bippavoq oh6~hqpou TOW uwhljva. Y ~ X E T ~ I ypljyopa U&

Q~ppo~pau ia 6wpaTiOU.

6.3.2. 0 pap~upaq Kal TO 6~iypa npo< avahuaq ~n&<&pya<ovral PE TOV i610 T ~ O T T O

~ a ~ a TO un6Ao1no pip05 T ~ S 6la61~aoia~. I lpoo-riB~v~a~ 10 ml ~U€ I~ IUT IKOO

61ah6pa~o~ ~ T T o u T ~ w ~ C ~ T O ~ (4.4) Kal ~ V ~ ~ E I ~ V ~ O V T ~ I .

6.3.6. r i v ~ r a ~ alfieqoq p ica an6 UTEYVO 61qeq~1~6 XUPT~, a r r~pp in~ov~a l ~a npL;)~a 2

ml, {avayiv~~al 61jOqur1, ~ a v x p ~ l a a ~ ~ i , Ewq OTOU TO 61rjeqpa y i v ~ ~ TEAE~WS

61auy i~ Kal U U M ~ ~ O V T ~ ~ 5 ml U& ~ O K I ~ C ( ( S T I K ~ uwhjva.

6.3.8. f l pou~ ie~v~a l 0,l ml 61ah6pa~oq BQC (4.6), avap~lyvirov~al, K ~ I TO xpdpa

acp j v ~ ~ a ~ va a v a n ~ u x 0 ~ i ~ r r i 30 h ~ r r ~ a BE B~ppo~pauia ~ W ~ C I T ~ O U .

6.3.9. METpdTal T) anopp6cpqoq UE U X ~ U ~ p& TOV iheyxo fi ' pdpTlJpCl UTO

cpaUpa~0cp~~6~E~p0 U& w f i K 0 ~ K U ~ . ~ ~ T O < 61 0 tlm (5.3).

6.3.1 0. 0 rrpocr61op1upo~ ~ n a v a h a p ~ a v ~ ~ a ~ p& ~ a ~ ~ h h q h r l apaiwoq TOU

6~iypa~oc ~ a v rl anopp6cpqoq, 6nws ~ E T ~ ~ € J ~ K E OTO uqp~io 6.3.9 un~ppaiva

Trlv anopp6cpquq TOU npo~unou nou n&plix&l 20 pg cpalv6Arlq avd uwhjva,

~ T T W ~ ~ E T P ~ ~ ~ K E o-ro oqy~io 6.4.4 . H apaiwoq a u ~ i n a p a u ~ ~ u a < ~ ~ a r p&

avap~ltq E V O ~ 6y~ou TOU 6~ i ypa ro~ n p o ~ avahuuq PE ~aTahhflh0 6 y ~ o p i p o u ~

TOU i610u 6&iypaT0< ~EppavefvTOq ~ P O U E K T I K ~ pfxpl ppa0p0c L~)UTE Va

a6pavono1qe~i q T T E ~ I E X ~ ~ E V ~ cpwacpa~auq .

6.4.1. flapacr~&ua<&~a~ ~a~dthhqhq o ~ ~ p d apalwpivwv rrpt~~urrwv, apx i<ov~a~ an6 TO

npo~uno cpalv6Aqq (4.8.2), nou r r ~ p ~ i ~ o u v 0 (Eh~yxoq j pap~upaq), 2 , 5 , 10 K ~ I

20 pg cpalv6hqq ava ~ lh loa~bh l~po . M~Tacphp~~Tal p& ulrpC;)vlo av~iu~olxa 1 m1

v&poO Kal 1 m1 an0 Ta ~ i o a ~ p a np6~urra 6lahirya~a cpa~vohqq oe KUQ& iva an6

T ~ V T & ~ ~ K ~ ~ ~ u T I K o ~ < CJWhflv&$.

6.4.2. ~POUT~@ETC~~'U& K U ~ E ~ O K ~ ~ ~ O T I K O owhjva 1 m1 61aAliparo< ~ E I I K O I ~ xahK0lj (11)

(4.7), 5 ml ~ U ~ ~ ~ I C ~ T I K O ~ 61ahOpa~o~ apaiwoq~ x p 6 ) ~ a ~ o ~ (4.3), 3 m1 v&poO Kal

0, l ml dlahOlJa~0< BQC (4.6)- avay c~yvliov~a~.

6.4.3. To xphpa arp ~ V E T ~ I va avarr~uxB~i ~ r r i 30 AEITTU UE B~ppo~pauia 6~paTiou.

6.4.5. An6 TI< T I ~ J ~ S anoppbqquq< (6.4.4) TOU ~hflcp8qoav yla K U ~ E ' T T O U ~ T ~ T U

cpalvoAq5 nou r r p o m i Q q ~ ~ (6.4.1), urroAoyi<e~a~ q ypapptj TraAlv6pbpqoqq 1.1~

T I l pie060 T U V E ~ ~ X I U T W V T E T ~ ~ ~ Y W V W V .

7.1.1. An0 TqV av6yvwor) TUC U T T O ~ ~ O ~ Q U Q S (6.3.9) ~TT0hOyi<&Tal q TrOU6TqTa TI)<

cpalv6hq~ ~ p q o ~ p o n o ~ h v ~ a ~ ~q ypapptj naA1v6p6pqoq< nou ~Aflcp8q (6.4.5).

(SUVTEAECTTI~S 2,4 ~ i v a ~ o o u v ~ ~ h ~ o ~ r j < apa iwaq~ (5112 1 ml B ~ i y p a ~ o ~ npoq

avahuuq) - B A i m oqp~io 6.2 UE auv6uaopo p& r a aqpda 6.3.2, 6.3.5 Kar 6

-3.6.

7.2 AKpiPEla

7.2.1. E-rravahqyrpo~qra (r): 2 pg cparvbAq<lml.

7.2.2. A v ~ T K I ~ ~ ~ w ~ ~ ~ o T ~ T ~ (R): 3 pg cpa~vohq~lml ( ' IT~oK~TC~~KTIK~) .

7.2.3. Eav xpqa1porrorq6~i pla apaiwoq oOpcpwva FE TO uqp~ io 6.3.10 TO bp10 nou

p v q p o v ~ O ~ ~ a ~ aTa o q v ~ i a 7.2.1 Kar 7.2.2 a v a c p i p ~ ~ a ~ oTa a n o ~ ~ h i o p a ~ a nou

n p o i ~ u y a v a n 6 TO apalwpivo 6 ~ i y p a .

1. AVTIKE~~EVO Kal T T E ~ ~ O ~qappoytj<

H p i 8 0 6 0 ~ a u ~ I j ~aBopi<~r ~q 6ra6r~aoia avacpopa$ ~rpoo61op1opoO T ~ S

napoua ia~ Ij a-rrouoia~ ~v<Opou u n ~ p o < ~ 1 6 a a q ~ OE yaha aav i va i h ~ y x o ~ r l ~

n a o ~ ~ p i w o q ~ .

Eav TO yaha EXEI ~ ~ O T E ~ I W ~ E ~ UWUT~, i va K U ~ V O xpwpa Ba T T ~ O K O ~ E I p ioa (SE

30 ~ E U T E ~ O ~ E T ~ T ~ an6 qv avap~~ t r l .

Kaviva xpwpa 6 ~ v 8a T ~ P O K L ~ ~ E I pioa UE 30 ~ E L J T E ~ ~ ~ E ~ T U an6 qv avap~~<q.

Alahdov~al 2 g 1,4-cpa1vuh~vo61apivr~ ( CSH~NZ ) UE @&pp6 ( SO0 C ) VEPO Kal o

o y ~ o ~ O U ~ T T ~ Q ~ W V E T ~ I iws sa 100 ml . To 61ahupa cpuAaoo&~ar o&

O~oupb~pwprl cp16h~ p& ~ U ~ ~ I V O n6pa Kal 6la~Qp&iTal OE ~ U X ~ O Kal OKOTEIVO

pipoc . MET^ qv n a p a a ~ ~ u q TOU, iva 61aAuga 1,4-cpa1vuh~vo61apiv~~

axqpari<&~ i va iQpa p ica a& pia f i 660 q ~ ~ i p ~ c : U U T ~ T T ~ ~ ~ E I p ~ ~ a va

anopptcp@&i.

Apa~wvov~al 9 m1 un&po<&16iou u6poyovou 30 % cr& v&po Kal o b y ~ o ~

U~pTrhQpdv~Tal iwq Ta 100 rnl. Tla o~a@~ponoiqoq, T T P O ~ T ~ ~ E T ~ I 1 ml n u ~ v o u

~ E I I K O I ~ o{toq ava hiTp0 61ahljpa~o~.

5.1. Eloayov~al 5 ml TOU 6EiypaToq ~ ~ ~ U K T O S FiUa U& Ka8ap0 60~lpCtCJ~l~6 owhtjva

C]& ~aTaAhT)ho Kh&ialpo.

5.4. flapa~rlp~iiar q napaywyrj xpQaioq pioa UE 30 ~ E U T E ~ O ~ E T T T ~ arro T ~ V

avap&l{q. Eav TO K U U V ~ X ~ W ~ U ITPOK~~WEI apy6T&pa an0 30 ~ E U T E ~ O ~ E ~ T T U a l l6

TqV ll~008fiKq TWV ~ V T ~ ~ ~ ~ ( S T ~ ) ~ I W V , t l aVif6pCfUq ~ E V pl'Top&i Va ~ a p € f K ~ ~ p l ~ ~ & f .

IV . KATAMETPHZH MIKPOOPTANIEMRN - AOKIMH METPHtHZ TPYBAIOY

ETOYE 30 'C

H pi80605 au~f i ~ a e o p i < ~ ~ T ~ V 6 ia61~a~ ia avacpop6~ ~ a ~ a p i ~ p q o q ~

p~~poopyavlal~bv TQ porj8~1a piaq TEXVIKI~S ~ a ~ a p i ~ p q a ~ ~ a n o l ~ i a ~ UTOUC

30 "C . H pi80605 ~ i v a ~ ~cpappoo~pq UE a ~ a ~ i p y a a ~ o yaha Kal narn~p~wp ivo

yaha Kal U& yaha UHT ~~r&{&pyaopivo Kal a n o u ~ ~ ~ p w p i v o npo~nwaopivo

o~ouq 30 "C &ni 15 11pip&~.

' E v a ~ KaeOplU~ i~0~ O ~ K O ~ TOU 6&iypUTO< ~ U ~ ~ K T O < ~ V ~ ~ E I ~ V ~ E T U I p& TO

U ~ O ~ T ~ W ~ U Tqs ~ahhl ipy~laq a& ~puphia Petri Kal &lTWd<&Tal DTOUq 30 "C ER^

72 W ~ E C . 01 allOlKi&~ KUTC~~ETPWVTUI Kal unohoyi<&~al 0 apl8p0< TWV

p~~poopyav~opwv ava rnl a ~ a ~ i p y a u ~ o u rj nao~~plwpivou y a h a ~ i o ~ rj ava 0, l

ml npo~nwaoytvou UHT &n&<~pyaoptvou 0 anoa~upwpivou V ~ ~ ~ K T O < .

4 . 'Opyava Kal yuahlva o ~ ~ l j q

4.1 . 'Opyava

4.1.1. Khipavoq B~ppoO atpa, r ~ a v o ~ va h ~ ~ ~ o u p y d UTOUS 170 h w ~ 1 7 5 "C.

4.1.9. AV~~&l~TfipCl<, I K ~ V O ~ VU U V ~ ~ E ~ ~ V ~ E I 1 m/ TOU 6&fypaT0< yahaKT0~ fi ~ E K U ~ I K ~ (

Up~iwCJq p& 9 m1 61ahli~q Kal ~ E ~ T O U ~ Y ~ V T U S T T ~ v w OTqV apxfl TqS EKK~VTPOU

TT&pla~pOtP~< TWV T T & ~ ~ & x o ~ E v w v TOU ~ O K I ~ U U T I K O ~ awhrjva .

4.2.2. @ ~ a h ~ q X W ~ T J T I K O T ~ ~ T U ~ 150 ~ W S 250 m1 fi CTwhfivE~, XWPT)TIK~TT)TU<

nepirrou 20 ml, yla va cpipouv TO un6a~pwpa ~Uhh l i py~ lC I~ .

4.2.3. Zlcpwvla (Trwpa~lopiva p& a ~ a ~ i p y a o ~ o PapPaKl) an6 yuahi fi arrocr~~lpwpivo

CTUV~ETIKO U~IKO, p& CIKipala UK~T), O V O ~ U U T ~ K ~ ~ S X W ~ I ~ T I K ~ T I ~ T U ~ 1 m1 Kal

ixov~aq arrohq<q 61ap i~pou 1,75 fwq 3 mm .

4.2.4. TpuPhia Petri, an6 61auyiq axpwpo yuahi fi a r r o o ~ ~ ~ p w p i v o OUV~ETIKO UAIKO,

p& TO a l l6 KUTW TpuPhi0 (nu8yiva) V U f x E l ECTWTEP~KI\ 6lapE~p0 T ~ E ~ ~ o u 90

i w ~ 100 mm. To. E C T W T E ~ I K ~ Paeoq T T ~ ~ T T E I va ~ i v a ~ TOUA~XIOTOV 10 mm . 0

-rru8pfvag 6 ~ v n p f r r ~ ~ va ~ X E I avwpahi~q nou va nap~pno6i?ouv sqv

K ~ T U ~ ~ T ~ Q C T I ' ~ TWV aTT0lKld~ .

(a) PE 61~1TfipT)Ur) (JTOU~ 170 i w ~ 175 "C ~n i 6x1 h~yb~epo an6 pic! wpa pdoa

CTE KhiPavo &pp00 aipa (4.1 . l)-

(P) IJ€ 6laTflpQOQ CTTOU< 121 k1 "C ETT~ 0x1 AIYOTE~O an6 20 ~ E T T T ~ pkJa CTE

~ U T O K ~ E ~ O T O (4.1.2).

Ta yuahlva (JKE6r7 TTOU ~ ~ ~ O U T E ~ ~ W ~ ~ K U V p ioa UTo C ~ U T ~ K ~ E I U T O npin&l va

(JTEYVWVOVT~I PE UTTUVT~T)UT) TOU U T ~ O ~ .

Ta OI(PWVI~ r r p i n ~ ~ va a n o a ~ ~ ~ p d v o v ~ a ~ ae KhiPav0 @&pp06 aipa (4.1 . l) .

5 . YTTboTpwva KahhlfpyElaq - Ayap yla ~Cl~apfTptlCJq TpuPhiou v& yaha

E~xljAlopa payrag 2,5 g

Tp UTTTOV~~ 590 g

D (+) yAu~o<q fi 6 & < ~ p b < ~ 1,o g

A~ro~ou~upwp ivo yaha a& 1,o g

O K ~ V ~

A V ~ P 10 fwq 15 g, avahoya p& T I ~

TTT)KTIK~< 1 6 1 6 ~ ~ ) ~ ~ ~ TOU

~pr)a1po~~o1o6p~vou ayap

N ~ p o 1 000 ml

To anopou~upwpfvo yaAa oE a~bvq T T ~ ~ T T E I va d v a ~ a-rrqhhaypivo an6

avamoA~i~. AUTO TTP~TTEI Va E ~ E Y X ~ E ~ p& ~ U ~ K ~ I T I K ~ ~ 60~1p.i~ x ~ ~ ) ~ I J J o T T o I ~ v T u ~

a-rropou~upwyivo ydha oe a ~ o v q TTOU ~ i v a ~ PE p&Palo~q~a anqhhayyivo an6

avao~oh~iq.

Ta DUOTCITIK~ 61aorr~ipov~a1 o~ a~wpqpa Kal 61aAljovsa1 p& qv a~ohoueq

~Elpa: E K X ~ ~ I U ~ J ~ ~.laylaq, T~uTTTov~~, y h u ~ 6 < ~ Kal T E ~ I K ~ , ~ T T O ~ O U T U ~ U ~ & V O

yaha UE OKOVQ, pfoa U& v&po. H Bhppavar) TOU arwptjpa~oq 8a poqQtjaa TQ

61a61~aaia au~ f i . I l poo~ ie~sa~ TO ayap Kal 8~ppaivsra1 p ixp~ ppaapou,

~ V U ~ E ~ O V T ~ S UUVEXL;)~ iwq ~ T O U TO ayap 61aAue~i EVTEAL;)~ fi a ~ p i < ~ ~ a ~ ~ n i 30

AElTTa TTEP~Tou.

Eav x p ~ ~ a u ~ d , y ivs~a~ 61lj0qoq pfaa an6 61q0q~1~6 xap~q.

Eav TO U ~ ~ O ~ T ~ W ~ U ~ E V TT~OKEITU~ VU X ~ ~ ( T I ~ O T T O I ~ ~ E ~ UIJ~OW~, (PU~~CTCJETU~ oTO

(SKOTU~I CTE 9~ppo~paa ia p~~a<Lj 1 Kal 5 'C ~ ~ r i 6x1 T T E ~ I ~ U O T E ~ O and i va p jva

and T ~ V .rrapaa~&uq.

To U T T ~ O T ~ W ~ U Kahhlfpy&la< (5.1) pnop&i va napao~&uao~&i an6 avu6po

UTrboTpwpa TOU ~pllOpf0U. A~ohouf3oirv~al 01 06qyiEs TOU KUTCIDKELJUDT~~, a h h ~

TO ~ T T O P O U T U ~ W ~ ~ V O yaha a& o ~ o v q n p o a ~ i e ~ ~ a ~ T T ~ I V 61ahu8~i av 6&v

~ T T O T E ~ E ~ CTU(TT~(STIKO.

TO pH E ~ ~ Y X E T ~ I pE hva p H p ~ ~ p o (4.1.4) E ~ V XPEI~OTE~, TO pH 610pB~V&~al hT0l

L;)uTE, ~ E T U TqV ~ T T ~ U T E ~ P W O ~ , Va EiVal 7,O 2 0,1 UTOU< 25 "C X ~ ~ U I ~ O T T O ~ ~ V T ~ <

61ahupa ( T O U ~ ~ X ~ O T O V 0,1 mOl/l ) u6po<&16iou V ~ T ~ ~ O U rj ~6poxhwp1~0ir 0<i0<.

Eav TO ~ ~ ~ I W T I K ~ ~ E V T T ~ o K E ~ T C ~ ~ va ~ptlUlp0TT0lqB~i a ~ i u w ~ , cpuhaoa&Tal m0

1 ~ ~ 0 ~ 6 6 1 CJE B~ppo~paoia p&~a<lj l Kal 5 ' C ~ r r i 6x1 TTE~IUCTOTE~O an6 hvav

prjva an0 Tnv n-apao~~vtj.

7. Aia61~aoia

7.1. T f i < ~ TOU UTTOUT~&~UTO<

7.3. Ilponolpaoia ~ q q apx~~f iq apaiwuqq (10-') ( a ~ a ~ i p y a a ~ o Kar .rracn&pwpivo

yaha)

M&Tacphp&~al PE aTT00T~lpWp6~0 alcpd~l0 (4.2.3) 1 m1 TOU ~ E ~ Y ~ ~ T O S (7.2)

a ~ a ~ i p y a a ~ o u ytihaK~o< tj rrao-rep~wpivou y a h a ~ ~ o ~ p ioa U& 9 ml a p a ~ w ~ ~ ~ o l j

(6.1) arro<p~ljyov~a< rqv ~rracpfi pe~atl j TOU aicpwviou Kal TOU apalwn~oir. H

B~ppo~paoia TOU apa~wnl<olj T T ~ ~ ~ T T E I va eiva~ Trepinou q i61a p& a u ~ i v TOU

6dyya~oq yaha~soq. H apx l~r j au~ t j apaiwaq avape~yvlje~a~ TT~OUEKTIKU UTOV

a v a p ~ ~ ~ t j p a (4.1.9) e n i 5 iwq 10 ~ E U T E ~ O ~ E T T T ~ .

'ET~I a r r o ~ ~ a ~ a ~ pia apxl~tj apaiwoq 10-'

7.4. f l apao~~u f l l l ~ p a l ~ h p ~ ~ E K ~ ~ I K W V ~PU~L;)U&WV (~K~TCPVQOTO Kal

nameplwpivo yMa)

M&Tacpfp€Tal FE ~ T T O U T E I P W ~ ~ V O ( ~ ~ ( P ~ v I o (4.2.3) 1 I l l 1 TqC ~ P X I K ~ ~ S a p a i w ~ q ~

(7.3) phaa D& 9 ml ~ ~ ~ ~ U T I K O U (6.1) ~ K O ~ O U ~ ~ V T U < 715 o6qyiq TOu 60e~Kav

OTO crqpeio 7.3.

7.5. El.rpoh~aopo< TpuPhiwv Petri

7.5.1. A K ~ T ~ ~ ~ ~ D T O ydha : p&Ta@p&Tal p& C ~ ~ O ~ T E ~ ~ W ~ ~ V O 0lcpLbVl0 (4.2.3) 1 m1 TOU

6&iypa~oq f l l~a l ~ q q ~a~ahhqhqq ~ E K ~ ~ I K & apaiuoqq piaa U& ~puphi0 (4.2.4).

~ P ~ T T E I Va E ~ E T ~ < O V T ~ I 660 a~alCi)~~&l< TOU~~XIUTOV. f l ~ 0 & ~ 0 l ~ a < E ~ a l 6va

TpUPhi0 and K&& apaiwoll ~a~ahhqha ~ n ~ h ~ y p i v q (8.1.1).

7.5.2. ~UUTEPIWJJEVO yaha : pE~a(p~p&Tal FE ~ T T O ~ T E I ~ W ~ ~ V O ( ~ ~ ( P ~ v I o (4.2.3) 1 m1 TOU

6~iypaT0< Q / K ~ I TqS ~a~ahhqhqq ~ E K ~ ~ I K ~ S apaiwoqq pioa UE TpuPhio (4.2 .4).

npfn&l Va & ~ E T ~ < O V T ~ I 660 apa lb~&l< T O U ~ ~ X ~ U T O V . ~ ~ o & T o ~ ~ ~ < o v T ~ ~ 660

TpuPhia all0 K ~ E U ~ U ~ W D T J ~a~ahhqha ET~I~EYIJ~VT) (8.1 .l),

7.5.3. raha UHT - ~ r r ~ c ~ p y a o p t v o Kal anocrr~~pwpivo. ( E ~ E T ~ < E T ~ I I J E T ~ an6 &nLbaoq

15 qp&pwv orouq 30°C). M~racpip~Tal p& a n o a ~ ~ ~ p w p i v o alcpLbvlo (4.2.3) 0, l

TOU 6Eiypa~0< TOU ydrha~T0~ (7.2) p&oa UE TpuPhi0 (4.2.4). ~ P O & T O ~ ~ ~ ~ < O V T ~ ~

660 ~puPhia .

Avap~1yv60v~ar api(Sw< p & ~ 6 TqV E K X U U ~ , ~ ~ E ~ I ( s T ~ ~ ~ o v T ~ < E T T ~ P K C ~ ) ~ TO

TpuPhiO Petri ~ U T E va arro~~qf306v opo16popcpa ~ a ~ a v ~ p q p i v ~ q a n o ~ ~ i ~ q ~ E T U

TqV ETTWO~T).

0 xpfrvoq ~ E T ~ { C TOUS T ~ ~ O U < Tq< ~ ~ o E T o I ~ J ~ C J ~ ~ S ; TOU 6&iypa~o< TOU yaha~TO<

Kal TqS ~ v u ~ E I < ~ ~ s , avahoya pE TO ~ i60q TOU yaha~TO<, Tq ~ O K I J J ~ C T T I K ~ ~ 60oq fi qv apaiworl TOU u-rroo~pwpa~oq 6&v ~ ~ ~ T T E I va u r r ~ p p a i v ~ ~ Ta 15 ~ E T T T U .

A q j v o v ~ a ~ va o~~peorrolq006v rravw UE ~aeaplj , lyuxpfl, o p ~ < o v ~ ~ a ~ n ~ c p a v ~ ~ a .

7.7. Enwaoq TUV TpuPhiwV Petri

Ta TpUPAia p~~acp ipov~a .~ UTOV ~ n w a o ~ r j (4.7.3). Ta TpuPhia ~ n w a < o v ~ a l

aV&~~pappiVCf. AEV O T O I ~ ~ < O V T U I ~ ~ E P I ( J O O T E ~ ~ an6 651 KaTdc thpO<. 01 aTjh&<

TWV TpuPhiwv n p f n ~ ~ va ai-rixouv q pia an0 ~qvahhq Kal an0 Ta ~ o ~ x c h p a ~ a KUI

TI)V KOPU(P~~ TOU ETTWUUT~~.

EKTEIVO~EVE~ ~TTOIK/E< ~ E W ~ O ~ ~ V T U I W< @VC< UTTOIK~E< . Eav h ly0~fp0 al l6 h a

TfTClpT0 TOU T P U P ~ ~ O U ~ X E I K U ~ U C ~ ~ E ~ an6 E K T E I V ~ ~ E V C < UTTOIK~ES, 01 U T T O ~ K ~ E ~

~a~ap~spo i r v ra l CTTO ~ T T ~ ~ C T P ~ ~ T O Tpfil~a TOU TpUPhiou Kal ~ J T T O ~ O ~ ~ ~ & T U I 0

apl9poq ~ o U C ~ V T ~ U T O ~ X E ~ (S& 0h6~hqp0 TO TpuPhio . Eav ~ E ~ I O ~ O T E ~ O an6 i va

T~TUPTO TOU ~puPAiou &&l ~ahu(pB&i an0 EKTCIVOCJEVE~ U~TOIK~E<, TO ~puPhio

U~TOPP~TTTETU~ .

8.1.2. 0 ap10~6q TWV pl~poopyav~apwv ava ml a ~ a ~ i p y a o ~ o u fi rracrr~p~wpfvou

~ ~ ~ U K T O S ~ ~ V E T U I am0 Tq axiar):

onou

ZC ~ivar TO a9p01opa TWV U ~ O I K I W V ~TTWC, K ~ T ~ ~ E T ~ O ~ T ) K ~ V U T ~ V

8.1.1,

(n~+O,l n2)d ~ i v a ~ icro PE TOV OYKO TOU 6Eiyp~T0~ UTO TpuApio, UTO onoio:

n l EiVal 0 ap lepb~ TWV TpuPAiwv TlOU K C ( T C X ~ E T P ~ ~ E ) ~ ~ K C I V (TTQV TFPWTI)

apaiwaq,

n2 ~ i va l o ap le~oq TWV Tpuphiwv TTOU ~ a ~ a p ~ ~ p f i e q ~ a v OTQ ~ E ~ T E ~ Q

apaiwoq,

H phpqoq 6iv~ra1 PE 660 U ~ ~ ~ V T I K U yqcpia. O ~ a v TO lyqcpio nou n p i n ~ l va

U T ~ O ~ ~ U ~ E U ~ E ~ dval TO 5, U T ~ O ~ ~ U ~ O ~ O I E ~ T U I CTUI WUTE TO lyq(~i0 UKPIPW< GTU

apl~TEp6 Va E~VUI T T E ~ I T T ~ .

Apaiwoq 10": 278 Kal 290 a n o ~ ~ i ~ q

Apaiwoq 10" : 33 Ka! 28 arrol~icq

8.1.3. Eav unapxouv povo p ~ ~ p l j a ~ ~ ~ ~IKPOTEPES an6 10, o ap~€lpo< TWV

pr~poopyav~apwv ava X I ~ I O ~ T O ~ I T ~ O avacpip~~al w< "~IKPOTEPO< an0 10 X d

ava ml", FE TO "d" va ~ i v a ~ TO av~io~pocpo TOU ~IKPOTEPOU UUVTEAEDT~~

apaiwoqs.

8.1.4. EUV UTT.U~XOUV v6vo ~ET~I)CTEI< TTOU UTTEPPU~VOUV TIS 300 ~ T T O ~ K ~ E S aAAa E~VUI

6uva~rj pi~prlatl, U T T . O ~ O ~ ~ ~ & T U ~ PIU K ~ T ' E K T / ~ I ~ Q p i ~ p t p r l Kal

Trohha~haala<&~al mi TO av~ia~pocpo TOU U U V T E ~ U T ~ apaiwa~<. To

anOTih&~pa avacptp~~al u s " E K T I ~ O U ~ E V O ~ aplf3p6~ p~~poopyav~opbv ava ml".

4486

V. KATAMETPHEH MIKPOOPrANIZMRN - AOKIMH METPHEHZ TPYBAIOY

tTOYE 21 oC

H pi60605 au~ l j ~a6opi<&1 rq 61a61~auia avacpopa~ ~ a ~ a p i ~ p q o q q

~ ~ K ~ O O ~ ~ ~ V ~ O ~ W V p& Tq pofi6~1a p1aS T E X V I K ~ ~ K ~ T C ~ ~ ~ T ~ Q U Q < alT0lKldV UTOUS

21 'C U& T K ~ U T E P ~ W ~ ~ V O yaha VET^ ail0 &ildC!Uq TOU ydrhaKT0~ UTOUS 6 'C ~lli

T ~ ~ V T E qpip&< yla va Trpoo61opla~~i o pa6pos p o h u v ~ q ~ TOU nam~plwpivou

y~haKT0~ an0 q ~ u ~ p o ~ p o n l ~ o l j ~ opyavl~polj< I K U V O ~ ~ ~ npo< TroAAaTrhaoraapb

U& yaAa o ~ o u ~ 6 'C.

TO Tram~plwptvo yaha ETIW~~<ETCII (JTOUS 6 'C &ITi TTEVTE pip&<. 'EV~G

~a0oplopivo< OYKOS TOU 6&iypaT0< TOU Y U ~ ~ K T O < aVap&lyV6&Tal p& TO

U T T ~ U T ~ W ~ U ~ r l q ~ a h h l i p y ~ l a ~ a& Tp~phia Petri K ~ I &-rrwa<&~al mou5 21 'C ~ T r i

25 L;)PES . 01 ~ T T O I K ~ E < K C ~ T C ~ ~ E T ~ W V T C ~ ~ Kal U T F O ~ O Y ~ < & T ~ ~ 0 a~l6p0< TWV

~ l ~ p 0 0 p y a V l ~ p 6 ~ avdr m1 ~ T ~ U T E ~ ~ W ~ ~ V O U ydtha~TO<.

4. ' Opyava K ~ I yuah~va U K E O ~

4487

4.1. ' Opyava

(a) 6+0,Z°C

(p) 2 1 f I 0 C

UE bha Ta u q p ~ i a TOU EUWTEPIKO~~ TOUS.

4.1.9. A V U ~ E I K T ~ ~ U < I K ~ V O < VU a~ap€lyVl~€l TOU 6€iypaTO< ~ U ~ U K T O < fi 6~Ka61Krj

61ahuuq PE 9 ml TOU 61ahli~ou KUI avap€iKTq< l ~ a v 6 5 o orroio< va ~EITOUPYE~

u ~ q v Paoq ~ r l < apxfic TQ< .rr~pla~pocprj< TOU r r ~ p ~ ~ x o p t v o u TOU urro ~£,i~aurl

Ei€iypa~o< UTov ~ O K I ~ ~ U T I K O uwhtjva.

4.2.2. @~ah&g X W P ~ T I K ~ T T J T ~ < 150 ~ W S 250 m1 0 OwhfiVE~, X W ~ T ( T I K O T ~ T U ~ TTEP~TTOU

20 ml, yia va cpipouv TO urroorpwpa ~ahhlipy~lag.

4.2.3. Zlcpwv~a (nwpa~iapiva FE a~a~ tpyauro pappdi~i) and yuahi r j a n o o ~ ~ i p w p i v o

OUV~ETIKO UAIKO, PE ~ K i p a l a 6~pt l , 0VO~aO~lKf i~ X W ~ ~ T I K ~ T ~ ~ T ~ ~ 1 m1 Kal

ixov~ag anoA~<q 6lapi~pOU 1,75 iwg 3 mm.

4.2.4. TpuPAia Petri, an0 61auyEq a ~ p ~ p 0 yuahi ~ ~ O U T E I ~ W ~ ~ V O U U V ~ E T I K ~ UAIK~,

C]& TO an6 KUTW ~ p ~ P h i 0 (TTuepiVa) va ~ X E I E U W T E ~ ~ K ~ ~ 6161pETp0 T T E P ~ O U 90-

100 mm To EOWTEPIKO Paeog T T ~ ~ T ~ E I va ~ i v a ~ T O U ~ ~ X I O T O V 10 mm. 0

nuepivaq 6 ~ v ~ r p i n ~ i va ~ X E I avwpahisg TTOU va ~rap~pn06i<ouv T ~ V

KaTaviTprlUQ TWV aTT01KlLbV.

Ta yutrhrva U K E ~ ~ Q TTP~STEI Va UTT~UTEI~L;)VOVT~~ p& pla CfTrrrb TIC a ~ 6 A o u 0 ~ ~

p~Q660uq:

(a) p& 61arflpqaq aroug 170 iwg 175 'C ~ n i 6x1 A I Y ~ T E ~ O an6 pla wpa p ioa

UE ~hipavo 0~ppol j a ipa (4.1 .l)

(P) FE 6laTfiprlOtl O T O U ~ 121 f 1 OC ~ n i 6x1 AIYOTE~O an6 20 A E ~ T ~ p iaa UE

C ~ U T ~ K ~ E I U T O (4.1.2).

f l p i n ~ i va AqcpeEi pipipva GUT& va &<aacpaA~o~&i ~ r r a p ~ t j g 61~ia6uoq arpou oro

~ U T ~ K ~ E I U T O : EUV 0 ef,onh~apog anou~&lpGv&~al a& 60x~ia, a u ~ a 6 ~ v T T ~ ~ T T E I Va

K ~ E ~ V O V T ~ I ( S T E Y ~ V ~ , 01 c p l ~ h ~ g TTP~TTEI Va ~ X O U V xahapa T O T ~ O ~ E T ~ ~ ~ V ~ TKbpaTa.

Ta yuahiva OKEljq l l O U ~ T T ~ ( ~ T E I P C ; ) ~ ~ K ~ V piOa UTO ~ U T O K ~ E ~ O T O TTpill.51 Va

U T E ~ V W V O V T ~ ~ PE ~TT~vThrlOrl TOU aTp0d.

Ta alcpLbv~a ~ ~ ~ T T E I va a r r o m ~ ~ p w v o v ~ a ~ CTE ~hipavo @&pp012 aipa (4.1.1).

5. YITOCITpwva ~ a h h l i p y ~ r a ~ - ayap yla KaTa~iTpq(Sq TpUPhiOU FE yaha

E~xljhlopa paylaq 2,5 g

Tpun~ovq 5 0 g

D (+) ~~uKo<~I 1,o g

A~ropou~upwpivo ydAa o~ a~6vq 1,O g

A V ~ P 10 h< 15 g, avahoya p& TIS T ~ ~ K T I K ~ S

1 6 1 0 ~ ~ ~ ~ ~ TOU X ~ ~ ( S I ~ O ~ O I O ~ ~ ~ E V O U

avap

N ~ p o 1 000 ml.

To anopou~upw~ivo yaha (SE a~ovr l npt-rr&l va ~ i va l anqhhay~ivo an6

avamoh~iq. AUTO n p i n ~ r va EAEXOE~ pc UUYK~ IT IK~< 60~1pis xpqa~pono~wv~aq

ano~ou~upwpivo yaha o& o~ovq nou ~ivar FE p ~ p a r o ~ r l ~ a a-rrqhhayviva an6

avao~ohdq.

Ta O U U T ~ T I K ~ ~ ~ I ~ D T T E ~ P O V T ~ I (SE CIlLhpqpa Kal 61ahljov~al v& TqV a~6Aou8q

(3Elpa: EK~6hlCIpa pavldr~, TpulTTbvq, yhu~6<q Kal TE~IKU, ~ T ~ O ~ O U T U P W ~ ~ ~ V O

yaAa (SE o~ovq, piaa UE v~po. H Oippavaq TOU a~wptjpa~oq 8a porlOr\u&~ ~q

61a61~auia au~t). npoo-rie~~ar TO ayap Kar B~ppa iv~~ar ~ ( i x p ~ ppaapoO,

a v a 6 ~ u o v ~ a ~ O U V E X W ~ f w ~ OTOU TO ayap 61ahuO~i EVTEAL~S fi CIT~~<ETCII ~ r r i 30

hurrra rr~pirrou.

Eav xp&laUT&i, ~ ~ V E T U I 6lfieq(Sq pioa an6 ~ I ~ ~ ~ ] T I K O XUPT~.

4490

5.2. Ka~avoprj, ~ ~ o u T E ~ ~ ~ u ~ Kal6la~t jpr l~q TOU Ull0UTp~paT0< ~ahhlipy&la<

To unbu~pwya (5.1) yo~pa<e~al ae nooo~q~ec; ~ w v 100 iw< 150 ml yioa UE

cp~ah~< Ij 12 i w s 15 ml pioa oe uwAljve< (4.2.2). 01 cp~ahe~ Kal 01 uwhljvc<

.rrwpa~i<ov~a~.,

Eav TO unoa~pwya 6 w T T ~ ~ K E T ~ I va xpqu1ponolq0~i apfuw<, (PUAUO(SET~I CTTO

U K ~ T ~ ~ I , UE ~ c ~ y o ~ ~ a o i a pe~atd I Kal5 oc e n i 0x1 T T ~ ~ I U B ~ T E ~ O am6 i va pljva

an6 T ~ V napau~eufl .

To unoo~pwpa ~ a h h l i p y ~ l a ~ (5.1) yrropei va mapaa~euao8ei an6 avu6po

unompwpa TOU E ~ T T O ~ ~ O U . A~ohou9oljv~al Ol o6qyicq TOU K ~ T ~ U K E U ~ O T ~ a h M

TO ~ T T O P O U T U ~ W F ~ V O yaha (s& UKOVQ llpOUTi0~Tal nplv 61ahuBei av ~ E V

~ T T O T E ~ E ~ UUBT~TIKO.

To pH rrpoaappo<c~ar aTo 6,9 rtr 0,1 DTIS 25 OC onw< rrcp~ypacpq~~ o ~ o oqpcio

5.1 Kal TO unou~pwpa ~ a ~ a v i p ~ s a ~ , ~ T T o C T T E I ~ ~ V E T ~ I Kal cpuhaaoe~al 6 n w ~

~~&plypa(pIl#& OTO or lp~ i0 5.2.

To a p a l w ~ l ~ o (6.1) ~ a ~ a v i y ~ ~ a l y iaa o~ 60~1yaas1~oi iq owhrjvq (4.2.1) a E

T ~ T O ~ E S TIOUOT~TE< WOTE, y&Ta TqV ~~ToQTEIPwCJT), Ka8& auhtjvas VU l l&pl ix€l

9,O + 0,2 ml a p a ~ w ~ ~ ~ o u . 01 a w h j v ~ q rrwpa~i<ov~al.

Eav TO apa lw i r~o 6 ~ v T T ~ O K E I T ~ I va xpqa1yon01q0~i a y i a w ~ , cp~ha~o&Tal aTo

aKo~dr61 o~ B ~ p y o ~ p a a i a j.i~~a<O 1 Kal 5 OC ~ n i 6x1 TTEPI~CT~TEPO an6 i va pqva

an6 T~IV n a p a o ~ ~ u Q .

To a p a l w ~ l ~ o (6.1) p r rop~ i va n a p a c s ~ ~ u a u ~ d an0 acpu6a~wyiva 6 l a ~ i a 0 D K ~ V E < TOU E ~ T T O ~ ~ O U . A K O ~ O U ~ O ~ ~ V T ~ I 01 o 6 q y i ~ ~ TOU KaTaaK~uaori. TO pH

~ I O ~ ~ W V E T ~ I OTTWC ~ T E P I Y ~ ~ ( P T ) K E UTO C T Q ~ E ~ O 6.1 Kal Ta apalwTlK6

~a rav ipov~a l , anoo7~1pWvov~a1 Kal c p ~ h ~ ~ o 0 ~ T a l onwq n ~ p ~ y p a q q ~ ~ CTTO

aqp~io 6.2.

7.2.1. ETTwa<&Tal pla K ~ E I U T ~ ~ u u o ~ ~ u a o i a n a u ~ ~ p ~ w p i v o u ~ M ~ K T o < fi, ~ a v a u ~ o ~ i v a l

a66va~0, iva ~VTITT~O(SWTI&UTIKO 6 ~ i y l a 6x1 A I Y ~ T E ~ O an6 100 mf &Tri 120 1: 2

wpe< UTOUS 6 +0,5 'C a& ~ r r h a o q [4.1.3.(a)].

M&Tacpip&Tal, pE a i r ~ ( S ~ & l p ~ l ~ i V O UI~~WVIO (4.2.3 ), 1 m1 TOU 6&iypaT0< (7.2.2)

aKClTfpyaUT0~ ydrhaKTO< fi T.ra~T&plw~f~OU ~ U ~ ~ K T O < pf(Sa U& 9 m1 Clpal~TlK06

(6.1) anocp~6yov~a~ T ~ V ~rracpfi p&ra<6 TOU o~cpLhv~ou Kal TOU aparw~1~06. H

B~ppo~paoia TOU a p a ~ w ~ ~ ~ o l j T T ~ ~ T T E I va rr~pirrou q i61a p& au~f i TOU

~E~V~.IUTOU< yaha~~oc;. H apxr~rj aurq aapaiwaq a v a p ~ ~ y v u ~ ~ a r R~OOEKTIK~ CTTOV

a v a p ~ l ~ ~ f i p a (4.1.9) &TTi 5 hw< 10 ~ E U T E P O ~ E ~ T C ~ .

'ETUI ~ T F O K T U T ~ I pla apxl~fi apaiwoq 10-'.

7.5 . El.tpokaap6~ TpUPhiwV Petri

A v C ! ~ & ~ ~ V U O V T ~ ~ apiCTw< p&Tdl TqV ~ K X U O ~ , lT&plCTTpi~OVTa<; &lTClpKh~ TO

TpuPhi0 Petri, Gcrrc va arro~~qeot jv opolopopcpa ~ a r a v ~ p q p i v ~ q a r r o ~ ~ i ~ q p ~ ~ a

T ~ V ~rrC;)acrq.

0 xpbvo~ p&Taf,fi TOU T ~ ~ O U S Tq< ~p~&TOl~ao iCf< TOU 6&iypClTO< y~hClKT0< Kal

~ q q avap&l<q~ apaiworlc Kal urro~r~p6paro< 6cv r r p i r r ~ ~ va u- r r~ppa iv~~ ~a 15

A&TTU.

Ta TpuPhia ~ E T U ~ ~ ~ ~ O V T ~ I oTov &lTwClCTTfi [4.1.3.(P)]. Ta ~puphia &nwa<ov~al

av~a~pappbva . AEV o ~ o l p a < o v ~ a ~ n ~ p l a a 6 ~ ~ p o an0 d51 KUTU 6 ~ 0 5 . 01 UTI)AE<

TWV ~puPhiwv n p f n ~ l va anixouv r) pla an6 qv cihhq Kal ano Ta ~ o l x & p a ~ a

Kal an0 TqV K O P U ( P ~ ~ TOU &llWClU~fi.

Ta TpuphiCI E<&TU<OVTC~I U& xapqho VWS . r la VU ~ I E U K O ~ U V ~ E ~ q K U T U ~ ~ T ~ ~ D ~ ,

pTTOp&i VU ~p~UlpOTTOl~8d KCfTdrMIlhO< ~ ~ U K O S (4.1.6) fi/Kal K U T U ~ E T ~ T ) T ~ ~ S

(4.1.8). ATTo(PE~Y&TuI q haveaoptvq B&wpqoq U W ~ U T I ~ ~ W V fi ~ a ~ a ~ a e l o p f v o u

U ~ I K O ~ UTU Tpuphia W< orlp&laKi< aTTolKi&<. E<&TU<OVTU~ TT~O(JEKTIK~( apcpipoha

a v ~ l ~ c i p ~ v a , ~pqo~l .~o-rro~wv~a< q a ~ o uyqh f i~ p&yi@uvoq< (4.1.7) onou

a n a l ~ e i ~ a ~ , yla va {exwpioouv 01 a n o l ~ i ~ < arro t i v o UAIKO.

EKT&IV~~JEV&< U ~ ~ O I K / & S ~ E W ~ O ~ V T U I W< povi< aTTOlKi&< . Eav ~ I Y ~ T E P O a750 bva

T ~ T U ~ T O TOU ~puphiou ~ X E I ~ahuqe&i an6 E K T & I V ~ ~ E V E < UTTOIK~E<, 01 C f l T ~ l ~ i & <

K U T U ~ & T ~ O ~ V T U ~ OTO aTTp0Oph~TO Tpfipa TOU TpUPhi0u Kal ~lTOh0y~ETal 0

aplep6< ITOU UVTICJTOIXE~ OE 0hoKhr)pO TO TpuPhio . Eav T T E ~ ~ O ~ O T E P O al l0 bva

T ~ U ~ T O TOU T ~ U P ~ ~ O U ~ X E I KCIhlJcp~~i an0 EKTE~VO~JEVE< UTTOIK~E<, TO TpuPhio

a r r o p p i n ~ ~ ~ a ~ .

8.1. X ~ ~ ~ U ~ ( J O T O ~ O ~ V T ~ I p&~ptjU&l< an0 oha TU TpUPhia TTOU TTEPI~XOUV an0 10 bw<

300 a n o ~ ~ i ~ ~ ( p h i n ~ ar lp~ ia 8.3 Kal 8.4).

8.2. 0 Clplep6< TWV ~ I K ~ O O ~ ~ U V ~ ~ ~ W V uvu m1 T T U O T E ~ ~ W ~ ~ V O U ~ U ~ U K T O < 6iV~Tal an6

onou:

1 C EiVal TO a8pOl~pCl TWV an0 l~ ldV 0 l T w ~ K U T U ~ E T ~ ~ ~ ~ K U V DTqV 8.1,

(nl + 0 , l n2)d Eival io0 p& TOV o y ~ o TOU 6&iypa~oq UTO TpuphiO, UTO onoio:

nl &iVal 0 ap18p6q TWV TpUphi~V l l O U K U T U ~ E T ~ ~ ~ Q K U V 0.Tq T T P , ~ T ~

apaiwaq,

n2 ~ivar o ap18p6q TWV TPUPA~WV nou ~ a ~ a p ~ ~ p i 8 q ~ a v aTq 6 ~ 6 ~ ~ p q apaiwaq,

d ~ i v a ~ o U U V T E A E ~ T ~ S apaiwoq< PE TOV onoio iylvav 01 T I~WTES ~ETPQUEI<.

H ~ . r h p ~ o q 6 i v ~ ~ a l p& 600 aqpavr l~a yqcpia . 'O~av TO yqcpio rrou T T ~ ~ T T E I va

U T ~ O ~ ~ U ~ E U ~ E ~ &iVal TO 5, O T ~ O ~ ~ U ~ O T T O ~ E ~ T U ~ ha1 WUTE TO l/Jqqi0 ClKplpLb~ aTU

a p ~ o ~ ~ p a va ~ i v a ~ TTEPITTO .

Apaiwaq 10": 33 Kal28 a n o l ~ i ~ q

=2,9 104.

8.3. Eav uTK?~)(ouv ~ O V O V p&Tpfi~&l< ~ I K ~ ~ T & P & < an6 10, 0 ap18p6q T U V

p~~poopyav~opwv ava X I A I O ~ T ~ A I T P O a v a c p i p ~ ~ a ~ wq " ~ I K ~ O T E P O S an6 10 X d

avdr ml, p& TO "dlt va t iva~ TO av~io~po(po TOU ~ I K ~ O T E P O U ~ U V T E ~ E U T I ~ apaiwoqq.

8.4. Eav unap~ouv .p6vo ~ E T ~ ~ U E I S nou un&ppaivouv TIS 300 ano l~ i&q ahha &ival

~ U V U T ~ ~ ll pi~pqDq, U~0hOyi<&~al pia K ~ T ' E K T ~ ~ I ~ u ~ ~ ~ T P ~ U T ] Kal

n o h h a n h a o ~ a ~ ~ ~ a l ~ n i TO ~ V T ~ ~ T ~ O ( P O TOU U U V T E ~ E U T ~ apaiwoq~. TO

arro~CA&opa avacpip~ra~ wq "EKTI~OU~EVO< ap10p6q p~poopyav~opwv ava ml .

V1 . KATAMETPHEH KOAOBAKTHPIAIRN - METPHZH AnOlKlRN ETOYf 30 'C

H pi0oBoq a u ~ r j ~ a 0 o p i < ~ 1 TU 61a61~aaia avacpopaq ~ a ~ a p f r p q u ~ q

~ohoPa~~qpliSiwv naas~p~wp ivo yaha p& ~q pofie~la plus T E X V I K ~ ~ ~

Ka~aphpIlCfq~ ~ ? T o J K ~ ~ v UTOUS 30 'C.

' Eva~ K U ~ O ~ I U ~ ~ V O S ~ ~ K O S ToU 6~iypaT0~ TOU y d h a ~ ~ o ~ avayslyvli~~al p& To

U T T O U T ~ W ~ U ~ a h h l i p y ~ l a ~ OE TpUPhia Petri Kal ETTWU<ETUI UTOUS 30 E lT i 24

L;)PEs. K U T ~ ~ E T P W V T ~ ~ 01 X U P U K T ~ ~ ~ O T ~ K ~ < alT0lKiE< Kal, EUV X~EIC((JTE(,

~lTlpEpCli~~€Tal q T U U T ~ T ~ T U TWV pq X ~ ~ U K T ~ ~ ~ U T I K L ~ V aTT0lKlbV C]& ~ A & Y X O TqS

~ K U V ~ T ~ T U ~ <lipWCJq< TrlS haKT6<~2. METU unohoyi<~rar 0 aplepbs

K O ~ O ~ U K T ~ ~ ~ I ~ ~ U V ~ V U m! T T U O T E ~ ~ W ~ ~ V O U ydhaKTO<.

4. 'Opyava Kal yuahlva o ~ ~ l j r l

0 UlJVqelo~iV0< &pyaUT~plCl~b~ ~<o-rrkop6$, Kal 16laiT~pa:

4.1. 'Opyava

4.1.3. E ITWU~TIK~ KhiPavo / incubator IKUVO va 6laTqpEi B~ppo~paaia 30 rt 1 OC CJE

oha Ta aqpda TOU EUWTEPIKOU TOU.

4.1.4. pHp&~po, J.IE 6uva~o~qta ~£,iowoq~ B~ppo~paaiaq, a ~ p i p ~ ~ a q +- 0,l pova6aq pH

4498

4.2.1. A O K ~ ~ U U T ~ K O ~ awhrjv&< PE ~a~ahhqho KhEiUlp0 Kal X W ~ ~ T ~ K ~ T ~ T U 20 ml, yla va

cptpouv TO urrou~pwpa &nlp&paiwaqq (5.2) Kal uwhfiv~q Durham K ~ T ~ A ~ ~ ~ A w v

~~uUTUUEUV yla xpfiUq PE TOU< ~ ~ K ~ ~ u ( s T I K o ~ ~ < UOhfiv&<.

4.2.3. Zcpwv~a (Tr~paTlUpE~a p& a ~ a ~ f p y a o ~ o Pap(36~1) an6 yuahi rj a n o o ~ ~ ~ p w p i v o

UUV~ETIKO U~IKO, PE aKfpala dr~pq, 0~0paUTlKfi< ) ( W ~ ~ T I K O T ~ ~ T U < 1-10 m1 Kal

Cxov~a< a-rrohq<rlGrapE~pou 1,75 iwc; 3 mm.

4.2.4. TpuPhia Petri, an6 61auyf~ axpwpo yuahi rj anoo~Elpwyivo U U V ~ E T I K ~ ) UAIKO,

PE TO an6 K ~ T W Tp~Phi0 (nu8pfva) va ~ X E I EOWTEPIK~~ 6 1 d p ~ ~ p 0 n~p inou

90-100 mm. To E O W T E ~ I K ~ pa00~ n p i n ~ ~ va ~ i v a ~ TOUA~XIUTOV 10 mm. 0

nu0pfva< 6 ~ v n p f n ~ l va ~ X E I avwpahi~q nou va rrapcpn06i<ouv TI~V

K~Ta!.liTpllUq TWV u ~ o ~ K ~ ~ v .

(a) p& 6laTrjp1loq mouq 170 iwq 175 ' C &ni 6x1 AIY~TEPO an6 pia wpa pfua

(SE KhiPavo 8~ppo0 afpa (4.1 . l )

(P) FE 61a~rjpqoq mouq 121 i 1 ' C ~ n i 6x1 AIYOTE~O an6 20 hEnTa pioa UE

UUTOK~&IUTO (4.1.2).

5.1. IO6&5-&pU8pb ayap X O A ~ ~ < - A ~ K T ~ < ~ < (ayap VRBL). E T & ~ & o E ~ E K T I K ~ U T T O O T ~ U ~ ~ .

.Iljo~aorl:

~ E T T T O V Q 7 g

E~xirhlupa pay la~ 3 g

AaKT6<r\ ( C12H22011.H~O ) 10 g

Xhwploljxo VUTPIO ( NaCl ) 5 9

XOAIKU aha~a 1,5 g

O U ~ ~ T E P O E P U ~ P O 0,03 g

Kpuu~ahhl~6 166q 0,002 g

10-1 5 g (avahoya PE TIC T T ~ K T I K ~ S I ~ I ~ T I J T E S

TOU X ~ ~ U I ~ O I T O I O O ~ ~ ~ E V O U ayap)

@~$OVTUI yptjyopa UE ppaopb, U V U ~ E ~ O V T ~ < K ~ T U 61ao~f lpa~a Kal apiuwc

K ~ T U V ~ ~ E V O V T U ~ UE T ~ o u ~ T Q T E < TWV 100 fw< 150 ml phaa U E ~ ~ T O O T E I ~ W ~ ~ V E <

(~lah&< (4.2.2). TO u~r6u~pwpa ~ J ~ X E T ~ I o& ~6p6hou~po (4.1.5) UTOU< 45 +l 'C.

H O T E I ~ ~ T Q T U TOU U ~ I K O ~ 7Tpf7TEl VU E ~ ~ V X E T U I TqV bpa TQ< ~pqUlp07~0iQUfi< TOU

(phfnf U Q ~ E ~ O 6.4).

Zdmaorl:

~ ~ E T T T O V ~ 10 g

A~KTo<Q (C12H220iiH20) 10 g

Xohtj p065 acpu6a~wpivq 20 g

Aapmpo rrpaolvo 0,0133 g

N ~ p 6 l 000 ml

TO unompwpa ' ~ a ~ a v i ~ ~ s a ~ , UE ~ O U O T ~ T E S TWV 10 ml, pioa UE ~ O K I ~ U U T I K O ~ ~ ~

uwhfivf~ (4.2.1) rrou r r~p lb~ouv o w h j v ~ ~ Durham. 01 owhtjv~< rrwva~i<ov~a~.