TrueAllele Challenges in Court and Culture€¦ · Cybergenetics © 2007-2012 2 Predict match...
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Cybergenetics © 2007-2012 1 TrueAllele ® Challenges in Court and Culture Cybergenetics © 2003-2012 9 th Annual DNA Technology Educational Seminar Centre of Forensic Sciences and the Promega Corporation Toronto, Ontario November, 2012 Mark W Perlin, PhD, MD, PhD Cybergenetics, Pittsburgh, PA, USA Commonwealth v. Foley Perlin MW, The Blairsville slaying and the dawn of DNA computing , in Death Needs Answers: The Cold-Blooded Murder of Dr. John Yelenic, Niapas A, New Kensington, PA: Grelin Press, 2012. Murder Victim's fingernails Minor 7% DNA mixture Crime: Evidence: Challenge: Statistic Method 13 thousand inclusion 23 million obligate allele 189 billion TrueAllele Defense: If mixtures are reliable, don’t we expect the same information from all methods? TrueAllele v. Inclusion information MW Perlin, A Sinelnikov. An information gap in DNA evidence interpretation . PLoS ONE, 2009.
Transcript of TrueAllele Challenges in Court and Culture€¦ · Cybergenetics © 2007-2012 2 Predict match...
Cybergenetics © 2007-2012 1
TrueAllele® Challenges in Court and Culture
Cybergenetics © 2003-2012
9th Annual DNA Technology Educational Seminar Centre of Forensic Sciences
and the Promega Corporation Toronto, Ontario November, 2012
Mark W Perlin, PhD, MD, PhD Cybergenetics, Pittsburgh, PA, USA
Commonwealth v. Foley
Perlin MW, The Blairsville slaying and the dawn of DNA computing, in Death Needs Answers: The Cold-Blooded Murder of Dr. John Yelenic,
Niapas A, New Kensington, PA: Grelin Press, 2012.
Murder Victim's fingernails Minor 7% DNA mixture
Crime: Evidence:
Challenge:
Statistic Method 13 thousand inclusion
23 million obligate allele 189 billion TrueAllele
Defense: If mixtures are reliable, don’t we expect the same information from all methods?
TrueAllele v. Inclusion
info
rmat
ion
MW Perlin, A Sinelnikov. An information gap in DNA evidence interpretation. PLoS ONE, 2009.
Cybergenetics © 2007-2012 2
Predict match information
15 loci
12 loci
67
info
rmat
ion
MW Perlin, A Sinelnikov. An information gap in DNA evidence interpretation. PLoS ONE, 2009.
Regina v. Broughton Arson, terror Fuse Low-level DNA mixture
Crime: Evidence:
Challenge:
• low template mixture • three DNA contributors • triplicate amplification • post-PCR enhancement
vWA locus
Likelihood ratio
Gen
oype
pro
babi
lility
(vW
A)
Assume coancestry at a theta value of 1%, and that there are three contributors present. A match between Mr. Broughton and the fuse is 3,620,000 times more probable than a coincidental match to an unrelated Caucasian.
6x
Perlin MW. Explaining the likelihood ratio in DNA mixture interpretation. Promega's Twenty First International Symposium on Human
Identification, 2010; San Antonio, TX.
Cybergenetics © 2007-2012 3
New York State Police validation papers
Perlin MW, Legler MM, Spencer CE, Smith JL, Allan WP, Belrose JL, Duceman BW. Validating TrueAllele® DNA mixture interpretation. Journal of Forensic Sciences. 2011;56(6):1430-47. Perlin MW, Belrose JL, Duceman BW. New York State TrueAllele® Casework validation study. Journal of Forensic Sciences. 2013;58(6):in press.
Information: sensitivity
MW Perlin, MM Legler, CE Spencer, JL Smith, WP Allan, JL Belrose, BW Duceman. Validating TrueAllele® DNA Mixture Interpretation. Journal of Forensic Sciences, 2011.
0
5
10
15
20
25
2A 2C 2H 2D 2B 2F 2G 2E
log(LR)
LR2CPI
7.03
6.24 13.26
Quantitative TrueAllele
Inclusion method
New York State Forensic Science regulatory approval
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Regina v. Duffy & Shivers Murder, terror Mobile phone, match stick Low DNA amount; mixtures
Crime: Evidence:
Challenge:
Real IRA claimed responsibility
March 7, 2009 Antrim, Northern Ireland
Combining DNA evidence
a
b
c
a
b
c
a b
a
c
singles pairs all three data
10 20 30
b
c a b c 1.1 million
6.04
STR tests
40,000
4.60
26,000
4.42
560
2.75
25
1.40
27
1.43
6
0.75
MW Perlin, Combining DNA evidence for greater match information, American Academy of Forensic Sciences 64th Annual Meeting,
Atlanta, GA, February 24, 2012.
TrueAllele match statistics
5.91 trillion
6.01 billion
1.1 million
Colin Duffy
Brian Shivers
Perlin MW, Galloway J. Computer DNA evidence interpretation in the Real IRA Massereene terrorist attack.
Evidence Technology Magazine. 2012;10(3):20-23.
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Admissibility ruling
… In Broughton, Judge Eccles did not give a reasoned judgment explaining his decision.
… I am satisfied that the stage has now been reached in the case of this system where it can be regarded as being reliable and accepted, and I am satisfied that Dr Perlin has given his evidence in a credible and reliable fashion. In the light of these conclusions I can see no basis on which I could properly exercise my discretion … to exclude this evidence, and I therefore admit it in evidence.
December 1, 2011 The Honorable Mr. Justice Hart
Pennsylvania precedent
Commonwealth v. Kevin Foley Pennsylvania Superior Court
February 15, 2012
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CPI information
Information log(LR)
Sta
tistic
log(
mC
PI)
Modified CPI information
Information log(LR)
Sta
tistic
log(
mC
PI)
SWGDAM 2010: DNA mixture interpretation guidelines
Interpretation of complex DNA mixtures must account for data uncertainty
TrueAllele and the crime lab
Casework system Interpretation services
Cybergenetics © 2007-2012 7
More information
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