Translation: From RNA to Protein

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Translation: From RNA to Protein

description

Translation: From RNA to Protein. Overall Picture. Protein. Universal Code. tRNA. To translate the mRNA transcript into a protein, the codons must be read one at a time to assemble amino acids in the right sequence This occurs in the ribosome with the help of transfer RNA ( tRNA ). - PowerPoint PPT Presentation

Transcript of Translation: From RNA to Protein

Page 1: Translation: From RNA to Protein

Translation:From RNA to Protein

Page 2: Translation: From RNA to Protein

Processed mRNA leaves the nucleus

mRNA mRNA binds to ribosomeRibosome

tRNA delivers amino acids

tRNA

Overall Picture

Protein

Page 3: Translation: From RNA to Protein

Universal Code

Page 4: Translation: From RNA to Protein

tRNA• To translate the mRNA transcript into a

protein, the codons must be read one at a time to assemble amino acids in the right sequence

• This occurs in the ribosomewith the help of transfer RNA (tRNA)

Page 5: Translation: From RNA to Protein

Structure of tRNAgluamino acid A tRNA molecule

bound to an amino acid is called an amino - acyl tRNA( aa-tRNA)

anticodon

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tRNA

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tRNA

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Structure of tRNA• Folded RNA molecule with an

anticodon loop– the anticodon contains a nucleotide

triplet which is complementary to the mRNA codon

• Each tRNA carries a particular amino acid corresponding to its anticodon

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Reusing tRNA

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How Many Codon Permutations?

AUCG

43 = 64

?

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Universal AND Redundant

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The Wobble Hypothesis• Though there are 61 codons coding for

amino acids, we do not need 61 types of tRNA

• The 3rd nucleotide in the anticodon is often less important than the first two(binds weakly / not specific)

• The third nucleotide is in the“wobble” position

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The Wobble Hypothesis• UCC and UCU both code for serine

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The Ribosome• A ribosome is a complex of protein

combined with ribosomal RNA (rRNA)• 2 subunits : small and large

3 binding sites for tRNA• P site

holds one aa - tRNA and the growing chain of amino acids

• A site receives the tRNA with the next amino acid to be added to the chain

• E site releases the used tRNA back into the cytoplasm

E P A

Small ribosomal subunit

Large ribosomal subunit

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Phases of Translation

1. Initiation

2. Elongation

3. Termination

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Initiation

1. In the cytoplasm a small portion of mRNA binds to rRNA on the small ribosomal subunit

2. A tRNA molecule with the start anticodon UAC (to complement the start codon AUG) binds to the mRNA - rRNA complex. The start tRNA carries the amino acid methionine

3. This complex then binds to the large ribosomal subunit

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Initiation

E P A

5´ 3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

U

P S

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PS

G

P S

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P S

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P S

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P S

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P S

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P S

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P S

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P S

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P

3’

5’

tRNA

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Initiation

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ElongationThe cycle of elongation has 3 steps:1. aa-tRNA binds to the A site.

2. The large ribosomal subunit catalyzes the formation

of a peptide bond with the previous amino acid. At the same time the polypeptide chain is passed from the tRNA in the P site to the tRNA in the A site. Translates 5’ 3’

3. Translocation: The ribosome moves 3 nucleotides (one codon) along the mRNA. This releases the used tRNA at the E site and frees the A site for a new aa-tRNA to bind

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Elongation

E P A

5´ 3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

U

P S

C

PS

G

P S

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P S

U

P S

A

P S

U

P S

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P S

A

P S

U

P S

C

P

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Elongation

E P A

5´ 3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

U

P S

C

PS

G

P S

G

P S

U

P S

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P S

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P S

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P S

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P S

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P S

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Elongation

E P A

5´ 3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

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P S

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PS

G

P S

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P S

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P S

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P S

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P S

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P S

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P S

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P S

C

P

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Elongation

E P A

5´ 3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

U

P S

C

PS

G

P S

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P S

U

P S

A

P S

U

P S

G

P S

A

P S

U

P S

C

P

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Elongation

E P A

5´ 3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

U

P S

C

PS

G

P S

G

P S

U

P S

A

P S

U

P S

G

P S

A

P S

U

P S

C

P

Growing polypeptide

chain

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Elongation

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Termination• There is no tRNA with the

complementary anticodon for the stop codon

• A protein release factor binds to the stop codon in the A site

• This cleaves the polypeptide from the tRNA and breaks apart the ribosomal sub-units

I love protein

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Termination

E P A

3´S

G

P S

G

P S

U

P S

U

P S

A

P S

G

P S

A

P S

U

P S

C

PS

G

P S

G

P S

U

P S

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P S

U

P S

C

P S

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P S

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P S

C

PS

U

P S

U

P S

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P S

C

P S

A

P S

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P S

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P

I’ll be back!

protein

mRNA

Release factor (protein)

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Termination

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Polysome• One mRNA can be bound

simultaneously to more than one ribosome. This is a polysome!(called a polyribosome in your textbook)

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Electron Micrograph

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3D Shape• During translation

polypeptides fold into a complex3D shape

• NOT DONE YET! Most newly synthesized proteins need modification in the ER and/or Golgi:

Post-translational modifications: - some amino acids may be removed- polypeptide can be divided into pieces- sugar and phosphate may be added- several polypeptides can join to form quaternary

structure

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Summary

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Homework

Your homework this evening is to:

1. Write captions for the diagrams provided

2. Do the section review questions on p. 331 #1-8

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Structure of tRNA

3’

5’

amino acid

anticodon

Ileu

A tRNA molecule bound to an amino acid is called an amino - acyl tRNA ( aa-tRNA)

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Polysome