Translation: From RNA to Protein
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Transcript of Translation: From RNA to Protein
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Translation:From RNA to Protein
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Processed mRNA leaves the nucleus
mRNA mRNA binds to ribosomeRibosome
tRNA delivers amino acids
tRNA
Overall Picture
Protein
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Universal Code
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tRNA• To translate the mRNA transcript into a
protein, the codons must be read one at a time to assemble amino acids in the right sequence
• This occurs in the ribosomewith the help of transfer RNA (tRNA)
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Structure of tRNAgluamino acid A tRNA molecule
bound to an amino acid is called an amino - acyl tRNA( aa-tRNA)
anticodon
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tRNA
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tRNA
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Structure of tRNA• Folded RNA molecule with an
anticodon loop– the anticodon contains a nucleotide
triplet which is complementary to the mRNA codon
• Each tRNA carries a particular amino acid corresponding to its anticodon
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Reusing tRNA
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How Many Codon Permutations?
AUCG
43 = 64
?
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Universal AND Redundant
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The Wobble Hypothesis• Though there are 61 codons coding for
amino acids, we do not need 61 types of tRNA
• The 3rd nucleotide in the anticodon is often less important than the first two(binds weakly / not specific)
• The third nucleotide is in the“wobble” position
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The Wobble Hypothesis• UCC and UCU both code for serine
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The Ribosome• A ribosome is a complex of protein
combined with ribosomal RNA (rRNA)• 2 subunits : small and large
3 binding sites for tRNA• P site
holds one aa - tRNA and the growing chain of amino acids
• A site receives the tRNA with the next amino acid to be added to the chain
• E site releases the used tRNA back into the cytoplasm
E P A
Small ribosomal subunit
Large ribosomal subunit
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Phases of Translation
1. Initiation
2. Elongation
3. Termination
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Initiation
1. In the cytoplasm a small portion of mRNA binds to rRNA on the small ribosomal subunit
2. A tRNA molecule with the start anticodon UAC (to complement the start codon AUG) binds to the mRNA - rRNA complex. The start tRNA carries the amino acid methionine
3. This complex then binds to the large ribosomal subunit
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Initiation
E P A
5´ 3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
G
P S
A
P S
U
P S
C
P
3’
5’
tRNA
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Initiation
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ElongationThe cycle of elongation has 3 steps:1. aa-tRNA binds to the A site.
2. The large ribosomal subunit catalyzes the formation
of a peptide bond with the previous amino acid. At the same time the polypeptide chain is passed from the tRNA in the P site to the tRNA in the A site. Translates 5’ 3’
3. Translocation: The ribosome moves 3 nucleotides (one codon) along the mRNA. This releases the used tRNA at the E site and frees the A site for a new aa-tRNA to bind
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Elongation
E P A
5´ 3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
G
P S
A
P S
U
P S
C
P
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Elongation
E P A
5´ 3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
G
P S
A
P S
U
P S
C
P
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Elongation
E P A
5´ 3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
G
P S
A
P S
U
P S
C
P
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Elongation
E P A
5´ 3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
G
P S
A
P S
U
P S
C
P
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Elongation
E P A
5´ 3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
G
P S
A
P S
U
P S
C
P
Growing polypeptide
chain
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Elongation
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Termination• There is no tRNA with the
complementary anticodon for the stop codon
• A protein release factor binds to the stop codon in the A site
• This cleaves the polypeptide from the tRNA and breaks apart the ribosomal sub-units
I love protein
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Termination
E P A
3´S
G
P S
G
P S
U
P S
U
P S
A
P S
G
P S
A
P S
U
P S
C
PS
G
P S
G
P S
U
P S
A
P S
U
P S
C
P S
A
P S
U
P S
C
PS
U
P S
U
P S
C
P S
C
P S
A
P S
A
P S
C
P
I’ll be back!
protein
mRNA
Release factor (protein)
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Termination
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Polysome• One mRNA can be bound
simultaneously to more than one ribosome. This is a polysome!(called a polyribosome in your textbook)
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Electron Micrograph
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3D Shape• During translation
polypeptides fold into a complex3D shape
• NOT DONE YET! Most newly synthesized proteins need modification in the ER and/or Golgi:
Post-translational modifications: - some amino acids may be removed- polypeptide can be divided into pieces- sugar and phosphate may be added- several polypeptides can join to form quaternary
structure
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Summary
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Homework
Your homework this evening is to:
1. Write captions for the diagrams provided
2. Do the section review questions on p. 331 #1-8
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Structure of tRNA
3’
5’
amino acid
anticodon
Ileu
A tRNA molecule bound to an amino acid is called an amino - acyl tRNA ( aa-tRNA)
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Polysome