Transgenic organisms powerpoint
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Transcript of Transgenic organisms powerpoint
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By Stephanie Wilson
The Creation of Transgenic Mice
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How do you create a Transgenic Mouse?
•Several methods but one most often used:
Transgenic Mouse Process Animation
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First Method of Transgenic Mice Creation
• (basic steps) Standard Transgenic Approach:1. Microinject fertilized mouse eggs with
transgene
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2. Injected eggs are implanted into a foster mother
Methods continued…
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Methods continued…
3. Offspring are then born “Pups”; possible founders (have the transgene)
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Method Continued…
4. Potential founders (pups with the transgene) are found using a PCR analysis and or southern blotting
(Black offspring = founders)
5. Founders can then be further analyzed for affects of that transgene
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Methods Continue…Transgenic Mice Technology Part 1
Transgenic Mice Technology Part 2
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Why create transgenic mice?
Transgenic Mice aids study of Pancreatic Cancer
•Transgenic Mice have:• Aided the study of Alchemies Disease• Aided in study of pancreatic cancer
• Acted as disease models • Tested toxicants • Produced specific proteins helpful in
biotechnology
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Pros and Cons • Pros
– Cheap– Short generation time– Defined genes
• Cons – Ethical Concerns
• Are the animals treated with respect?– Environmental Issues
• What if a genetically modified animal gets loose?– Religious Views
• Do we have the right to act as God?
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Animation And Picture Credits• Experiments." Experiments. Web. 23 May 2012.
<http://student.biology.arizona.edu/honors2005/group07/Experiments.html>.• "Transgenic Mice." Transgenic Mice. Web. 23 May 2012.
<http://www.accessexcellence.org/RC/VL/GG/transgenic.php>. • Creating Transgenic Mice: On-Line Self-Study." Human Development. Professor Danton O'Day.
Web. <http://www.utm.utoronto.ca/~w3bio380/pdf/Creating%20Transgenic%20Mice.pdf>.• "Transgenic Mouse Animation." Transgenic Mouse Animation. Web. 23 May 2012.
<http://spine.rutgers.edu/cellbio/assets/flash/transgen.htm>.• Transgenic Animals. 13 June 2011. Web.
<http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html>.• "Fig002jme: Two Methods to Produce Transgenic Mice." Fig002jme: Two Methods to Produce
Transgenic Mice. Web. 23 May 2012. <http://journals.cambridge.org/fulltext_content/ERM/ERM3_12/S1462399401002952sup004.htm>.
• Proneural. "The Science of Transgenic Technology Part 2 of 2." YouTube. YouTube, 25 Apr. 2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=EFNtTfhhQ70>.
• Proneural. "The Science of Transgenic Technology Part 1 of 2." YouTube. YouTube, 25 Apr. 2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=ujZHrR1mro8>.