Transcriptional Specificity of Dysfusion

Dys Forms a Heterodimer with Tgo In Vivo

Ectopic Dys Expression cause ectopic Tgo nuclear Localization

Dys::Tgo Activates TCGTG, GCGTG and ACGTG in S2 Cell Culture

Nxf::Arnt and Nxf::Tgo Activates TCGTG,GCGTG and ACGTG

Trh:Tgo Activates ACGTG

Sim::Tgo Activates ACGTG

Basic region alignment of selected bHLH-PAS proteins

Protein Basic region Binding site D-Dys NKSTKGASKMRR AC, GC, TC H-Nxf YR•••••••A•• AC, GC, TC D-Sim KEKS•N•ART•• AC H-SIM1 KEKS•N•ART•• AC D-Sima KEKSRD•ARC•• AC H-HIF-1 KEKSRD•ARS•• AC M-HLF KEKSRD•ARC•• AC D-Trh KEKSRD•ARS•• AC D-Ss GVTKSNP••RH• GC H-AHR EGIKSNP••RH• GC

NC/GTG

Identification of Dys taget gene CG13196 Enhancer

Fusion cell expression of CG13196 requires TCGTG sequences

Misexpression of dys results in ectopic expression of Dys target gene CG13196 in multiple cell types