“Standardization of Stem rust note taking and evaluation of germplasm with emphasis on emerging

threats of Yellow rust and Leaf rust”Kenya , Njoro from the 12 – 18 October 2015

Training course on

The annual training course is part of the Durable Rust Resistance in Wheat (DRRW) project funded by the Bill and Melinda Gates Foundation and the Department for International Development (DIFD, UK( .

Atef A. Shahin, Ph.D.Wheat Disease Research DepartmentPlant Pathology Research Institute

ARC, Egypt

7th Annual training course on Standardization of Stem Rust Note-taking and Evaluation of GermplasmKalro, Njoro, Kenya (12th-18th October, 2015)

Course ObjectiveThe course aimed to create awareness of the devastating effects of stem rust race Ug99 on global wheat production; train wheat researchers to use new approaches to fight the rust diseases (including genetics, pathology, breeding, and molecular genetics); and teach them methods for identifying, scoring, and evaluating stem rust both in the field and experimental plots. Practical sessions focused on demonstrating rust methodologies and giving participants hands-on experience in scoring the disease both in the greenhouse and the field. “They also evaluated materials from their own national programs to know the status of resistance and learned breeding approaches to develop resistant varieties”The annual training course is designed to monitor further migration of Ug99 and its variants; facilitate field screening of international germplasm; identify new sources of resistance; understand the genetic basis of resistance; develop durable, targeted breeding programs; and enhance the capacity of national programs.

Training Program

Training Program

  

 

 

 

 

 

 

 

 

 

 

 

 

Standardization of Stem rust note taking and evaluation of germplasm with emphasis on emerging threats of Yellow rust and Leaf rust

Total of 32 participants from 12 countries around the world….

Countries ParticipantsHungary 1Egypt 2Nepal 1India 4Iran 3Pakistan 11Ethiopia 3Kenya 3

Bangladesh 3Turkey 1

For the seventh consecutive year, scientists from around the world met at the Kenya Agricultural Livestock Research Organization (KALRO-Njoro) for training on “Standardization of Stem Rust Note-taking and Evaluation of Germplasm.” The week-long course (12-18 October 2015) attracted 32 participants from institutions in 12 countries, including Bangladesh, Egypt, Ethiopia, Hungary, India, Iran, Kenya, Mexico, Nepal, Pakistan, Turkey, and USA.

Gordon Cisar, Ph.D., (DRRW, Cornell University)

The Durable Rust Resistance in Wheat project and the Borlaug Global Rust Initiative

• Origin of the project (Sr31 & Ug99)• Raising awareness and organizing a response• Funding the project ($ from BMGF & DFID)• Objectives of the DRRW project• Rust never sleeps• GOAL: A durable rust research community

406 page proposal accepted Feb., 2011

DRRW Project

On behalf of the collaborators of the DRRW project, welcome to the challenges of the global wheat rust community..

..so welcome to the 7th KARI-Njoro rust training workshop..Please ask questions. We will all learn from the discussion.

Keep in touch: www.globalrust.org

Pathogenicity Surveys and Nomenclature

Pathogen Genetics

Mendelian Genetics

R. McIntosh (Prof. Emeritus, University of Sydney)

HOST : PATHOGEN GENETICS

THE GENE-FOR-GENE CONCEPT

LAWS OF HOST:PARASITE INTERACTION

Basic Experimental Models Used in Host:Pathogen Genetics

Wheat Rusts- Overview and Importance

Enhancing wheat productivity and sustainability in Asia, Africa and Latin America by developing and deploying higher yielding, climate resilient and

disease resistant varieties

Ravi P. Singh and Spring Bread Wheat Improvement Team, Global Wheat Program

Speaking at the end of the training, Ravi Singh, Chief Wheat Breeder and Distinguished Scientist, CIMMYT, emphasized that global collaboration and networking are vital in addressing global challenges such as stem rust Ug99.

A Decade of Stem Rust Surveillance: How far have we come and where are we going?

Dave Hodson, CIMMYT-Ethiopia 2005: What did we need to do?

•Build the surveillance network (surveys + sampling) •Track the important pathogens – inform breeding programs •Manage + disseminate information •Improve impact assessments •Put some early warning systems in place Survey Tools: New Technology Options Data Management: Wheat Rust Toolbox Wheat Rust Toolbox ICT framework Public Information Systems :

WWW Rust Tracker.org Successful Pathogen Tracking Ug99 Race Group Molecular diagnostics and population genetics Emerging Early Warning Systems

Under the guidance of Dave Hodson, CIMMYT Senior Scientist, participants acquired hands-on experience using surveillance tools and GPS for conducting informed pathogen monitoring and surveillance, and evaluating and recording stem rust data.

They also observed CIMMYT-Kenya shuttle breeding activities aimed at incorporating Ug99 resistance into CIMMYT germplasm and progress in breeding for durable adult plant resistance.

CIMMYT-KARI-DRRW partnership in East Africa: Working together to beat the threat of Ug99

Sridhar Bhavani

Gene Identification and characterization Mapping Major genes Fine mapping

Breeding for resistance to stem rust Breeding options available Few in high yielding adapted backgrounds, e.g. Sr22, Sr25, Sr26,Sr33,Sr45,Sr50,SrNd643, SrNiini, SrYaye, SrHuw234 .

Stacking/Pyramiding multiple race specific genes (Sr22, Sr25, Sr26)

Effectiveness of Known Sr genes to TTKS (Ug99) Lineage

Enrique Autrique Godwin Macharia

Ruth Wanyera

High throughput phenotyping HTP - BW Breeding HTP - BW Breeding

PhenocornSPAD Canopy

HTP for predicting yield Accurately predicting yield prior to testing could increase

rates of genetic gain

Yield prediction accuracies were measured using

HTP Genome-wide markers (Genomic selection)

Pedigree records

separately and in combination

Training the Next Generation of Rust Researchers to Combat Stem Rust Race Ug99

Certificate distribution to trainees: Sridhar Bhavani (CIMMYT), Immaculate Maina (Center Director, KALRO) and Atef Shahin (trainee from Egypt). Photo: Marianna Myer.

Participants acknowledged the valuable knowledge and experience shared by rust researchers including Bob McIntosh (University of Sydney), as well as CIMMYT scientists Ravi Singh, Dave Hodson, Julio Huerta, among others.

Trip to Nakuru National Park

Photo: Marianna Myer .

 

 

 

 

 

 

 

 

  

 

 

 

 

 

 

 

 

 

 

  

 

AcknowledgementsImmaculate Maina, Director, Njoro Station, Kenya R. McIntosh (Prof. Emeritus, University of Sydney), Ravi Singh (CIMMYT-Mexico) Gordon Cesar (DRRW, Cornell University) Sridhar Bhavani (CIMMYT) Dave Hodson (CIMMYT-Ethiopia) Durable Rust Resistance in Wheat (DRRW(Bill and Melinda Gates Foundation Department for International Development (DIFD, UK(

Contact : a.a.shahin@hotmail.com

Thanks to all the people who have contributed to organizing this training course

7th Annual training course on Standardization of Stem Rust Note-taking and Evaluation of GermplasmKalro, Njoro, Kenya (12th-18th October, 2015)