TP900 v4.01E Instrument Manual Manual/2... · 2020-01-16 · 1 Precautions for Using TaKaRa...

Model TP700/760

Software version: V5.0x OS: Windows XP / Vista / 7

Instrument Manual

TAKARA BIO INC.

Attention: Thermal Cycler DiceTM

Real Time System Software Operation Manual

should be stored for reference in a secure and accessible location.

TM

NOTICE

P10 PCR Notice

Purchase of this instrument conveys a limited non-transferable immunity from suit for the

purchaser's own internal research and development and applied fields other than human in vitro

diagnostics under one or more of U.S. Patents No. 5,475,610 or corresponding claims in their

non-U.S. counterparts, owned by Applera Corporation.

No right is conveyed expressly, by implication or by estoppel under any other patent claim.

Further information on purchasing licenses may be obtained by contacting the Director of

Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Applied Biosystems does not guarantee the performance of this instrument.

L45 Heated Cover

This product is covered by the claims of U.S. Patent 5,552,580, and their corresponding foreign

counterpart patent claims.

L47 Real-Time PCR Quantification Method

The purchase of this product includes a limited, non-transferable license for all fields other than

human or veterinary in vitro diagnostics under specific claims of U.S. Patent Nos. 6,174,670,

6,569,627, 6,303,305, and 6,503,720, owned by the University of Utah Research Foundation

and licensed to Idaho Technology, Inc. and Roche Diagnostics GmbH.

- Thermal Cycler Dice™

is a trademark of TAKARA BIO INC.

- Microsoft® Windows

® XP , Windows Vista

® , Windows

® 7 , Excel

® , PowerPoint

®

is a trademark of Microsoft Corporation.

- Adobe® Reader

® is a trademark of Adobe Systems Incorporated.

- All marks are the property of their respective owners. Certain trademarks may not be

registered in all jurisdictions.

Copyright© 2012 by TAKARA BIO Inc., Otsu, Shiga, Japan

TP700/760 Instrument Manual

Rev. A

＝ Contents ＝

page

Precautions for Using TaKaRa Scientific Instruments --- 1

Chapter 1: Outline --- 6

Chapter 2: Installation Requirements and Maintenance --- 8

1. Installation Conditions --- 8

2. Opening the Packaging --- 9

3. Installation -- 11

4. Moving the System -- 12

Chapter 3: Part Names and Functions -- 13

Chapter 4: Explanation of Screens -- 17

1. System Startup and Shut Down -- 17

2. Initial Screen Menu -- 19

Chapter 5: Maintenance -- 22

1. Self-diagnostic Function -- 22

2. Optical Calibration -- 25

3. Daily Inspections -- 36

Chapter 6: Troubleshooting -- 37

Chapter 7: Specifications -- 42

Chapter 8: Consumption and Options List -- 45

Appendix : Thermal Cycler Dice Real Time System TP700/760 -- 46

1. Computer Driver Setup -- 47

2. Software Install / Uninstall -- 52

3. Computer Basic Setup -- 56

1

Precautions for Using TaKaRa Scientific Instruments

●Users are recommended to carefully read the content of this manual before operating the TaKaRa PCR

Thermal Cycler Dice system. ●After familiarizing themselves with the information in this manual, users are recommended to store it in

secure and convenient location from which it is always available for reference

Introduction

All Takara scientific instruments are sold strictly for use in research applications and should not be

used by those who are not experts in this field.

Due to ongoing improvements, product specifications and the external appearance are subject to

change without prior notification.

Warranty and Responsibilities

Refer to the Warranty card included with this product for detailed description of the Warranty

conditions and period.

This product is designed to ultimate safety standards. Nevertheless, should any safety-related issues

arise, users are requested to immediately contact the nearest sales agent.

Precautions (safety and accident prevention) for System Use

（１）Danger Warnings

The various messages and labels provided on the product itself and in the various manuals are

designed to ensure the system is operated safely to prevent personal injury or damage to property.

Users are strongly recommended to read and observe the warnings included with the symbols

shown below.

Warnings indicate incorrect system operation and handling procedures that can lead to serious accidents.

Cautions indicate incorrect system operation and

handling procedures that can lead to accidents. ！ CAUTION

！ WARNING

2

（２）General Precautions Regarding Takara Scientific Instruments

It is important to observe the various precautions described below to ensure optimum system

performance and prevent accidents before they happen. Ensure safe and correct system operation

by carefully reading the precautions.

【Precautions for Installation Location】

Observe the following precautions in order to prevent fires and electrical shocks.

● Do not install the system on an unstable or sloped foundation or any

other location subject unstable conditions.

● If the system tips over or falls, immediately turn off power to the

system and all connected devices and disconnect the power supply

cables.

● Install the system away from direct sunlight or rainwater.

● Avoid blocking the paths to any air vents included on the product.

Blocked air vents can lead to internal overheating that may result in

physical burns or fire.

● Avoid locations subject to corrosive or flammable gases.

● Avoid installing the system in locations subject to dust or strong

vibrations.

● Avoid installing in locations subject to direct sunlight and wide

temperature fluctuations as this may result in internal overheating and

may cause physical burns or fire.

● Do not place objects on top of the system.

● Do not install the system in locations other than research institutions or

related facilities.

【Electrical Precautions】

Observe the following precautions when using electrically powered scientific instruments in order to

prevent electric shocks and fires.

● Avoid operating system with power sources that do not conform to

system power supply specifications.

● Avoid sharply twisting, altering, marking or otherwise damaging the

power supply cable. Avoid applying stress to, heating or placing

objects on the power cable.

● Never use a multiple outlet extension plug.

● Always ground the system to prevent electric shocks due to leakage

current.

！ WARNING

！ CAUTION

！ WARNING

3

● Be sure to compare system power supply requirements with the rated

power capacity of the power source to ensure system current

requirements do not exceed power socket capacity. Confirm that the

total power requirements of all connected equipment do not exceed

the rated power supply capacity of the common power source.

● The system body and power supply cable are subject to high voltage

levels(The maximum voltage is a line voltage AC100V～240V). Avoid

inserting metal or flammable objects inside the system or handling it

with wet hands, as this can result in electric shock and/or other

dangers.

● When moving the system, be sure to first disconnect the power cable

as well as any peripheral devices.

● Be sure to disconnect the power cable prior to cleaning or servicing

the system.

【Other precautions】

Observe the following precautions in order to prevent electric shocks and fires.

● Avoid inserting metal objects, flammable materials or other foreign

objects into the various openings on the system case.

● Never remove the system cover or attempt to alter it in anyway.

● Avoid disassembling or making any direct contact with internal parts of

the system.

● Should noxious fumes smoke or other abnormal signs appear,

immediately terminate operation, turn off system power and

disconnect the power cable.

Wait until conditions stabilize before contacting the nearest sales

agent or the company technical support line.

● Should water or other foreign matter enter the system, immediately

terminate operation, turn off system power and disconnect the power

cable.

Wait until conditions stabilize before contacting the nearest sales

agent or the company technical support line.

● Do not sit on the system.

● Do not disassemble the system

！ WARNING

！ CAUTION

！ WARNING

！ CAUTION

4

（３）Warning Safety Labels Applied to System Case

Users are advised to avoid accidents by carefully reading the warnings and cautions contained on

warning stickers at key locations on the interior and exterior of the system.

Possible

Danger

Warning Type

Location of Danger

Type d’Avertissement

Localisation du Danger

Warning Label Description of

Danger

Electric

shocks and

fires

Disassembly

Prohibited

System Interior

Démontage Interdit

Intérieur de l’Appareil

Never disassemble

system, as this may

lead to electrical

short-circuits,

leakage current

and/or fire.

Electric

shocks

Warning! High

Voltage

Power cable

Attention! Haute

tension

Cordon d’alimentation

The case and power

cable are subject to

high voltage levels,

so take care to avoid

electric shocks.

Electrical

shocks and

fires

Grounding Mandatory

Power cable

Mise à la Terre

Obligatoire

Cordon d’alimentation

Always ground the

system to prevent

electric shock from

leakage current.

Burns High Temperatures

Heat cover

& Heat block

Haute Température

Couvercle Chauffant

Avoid touching the

heat cover, which

reaches very high

temperatures and

may cause burns.

Burns and

fires

Installation

Precaution

Air vents

Précautions

d’Installation

Prises d’air

Be sure not to block

air vents when

installing the system,

as this can cause

internal overheating.

Personal

injury

Do not Open When in

Operation

System cover

Ne Pas Ouvrir en

cours de

fonctionnement

Couvercle

Be sure to close the

cover while the

system is in

operation. Failure to

do so can lead to

burns and/or

damage to the eyes.

5

This symbol means caution or warning.

This symbol means an action is prohibited.

This symbol means an instruction must be followed.

（4） Electrical Symbols

The symbols shown on this page are adapted to the TaKaRa PCR Thermal Cycler Dice. Read the

explanations and make sure you understand what the symbols mean before you interact with the

instrument in any way.

This symbol indicates the on position of the main power switch.

This symbol indicates the off position of the main power switch.

Precautions against Transferring or Copying Content of This Document

The content of this document cannot be transferred or copied without the express permission of the

copyright holder.

Copyright holder: TAKARA BIO INC.

Printed in Japan

Contact TAKARA BIO INC.

TAKARA BIO INC.

SETA3-4-1, OTSU, SHIGA, 520-2193, JAPAN

http://www.takara-bio.com

Technical Inquiries (Contact):

URL: http://www.takara-bio.co.jp/english/index.htm

http://www.takara-bio.com/

http://www.takara-bio.co.jp/english/index.htm


Rev. A

6

● Chapter 1: Outline

Thank you for purchasing the Thermal Cycler Dice™

Real Time System Lite.

This system consists of the following components:

<TP700> Thermal Cycler Dice™

Real Time System Lite

1. Thermal Cycler Dice™

Real Time System Lite Main Unit (Code: TP710)


Real Time System Control Computer (Code: TP820)


Real Time System Software for TP700 (Code: TP730)

<TP760> Thermal Cycler Dice™

Real Time System Lite MRQ


Real Time System Lite Main Unit (Code: TP710)


Real Time System Control Computer (Code: TP820)


Real Time System Software for TP700 (Code: TP730)

4. Multiplate RQ Software (Code: TP840)

System construction table

System Lite code TP700 TP760

TP710 Dice Real Time System Lite Main Unit ○ ○

TP820 Control Computer ○ ○

TP730 Dice Real Time System Software for TP700 ○ ○

TP840 「Multiplate RQ」 Software － ○


Rev. A

7

The main features of the Thermal Cycler Dice™

Real Time System Lite are as follows:

【 TP700/760 】

This model has the 48-well Sample Block Assembly. Use a 48 well plate, 24well

plates, 8 strips tubes and individual tubes for your situation.

The reaction block of copper with a high thermal conductivity is use. Temperature

uniformity has improved better than the reaction block of aluminum.

Original calibration processing eliminates the need for calibration using secondary

fluorescent dye. This reduces running costs and reagent preparation time.

The miniature design saves space by using the smallest width and height possible.

With an internal mirror and built-in tilt and sliding lid mechanism, the device can be

stored on a shelf, but is still easy to operate.

( Main Unit Dimensions: Width 294 x Depth 480 x Height 304 [mm] )

The CCD camera system gives no difference in measuring time between wells.

The system includes automatic compensation for stable detection, maintenance

functions and easy optical calibration functions.

Expandability is provided for by allowing installation of a maximum of four (4) types

of filter unit. Two fluorescent filter units are installed as standard (FAM and ROX).

The application software is user-friendly. Basic settings are easy, and application

settings provide a high degree of freedom. It is possible to set the fluorescent

measurement point and to view data even with other computers. The software

uses a unique data format that prevents alteration of the data by general-purpose

software.

The application software have the Absolute Quantification method, the Relative

Quantification mode and added the Plus/Minus Assay program and the SNP

Genotyping Assay program.

The application software has the List manager of Target and Sample, making text

files for RDML(Real-time PCR Data Markup Language) and changeable graphic

colors patterns.

【 TP760 only 】

TP760 system includes Multiplate RQ software ( Code: TP840).


Rev. A

8

● Chapter 2: Installation Requirements and Maintenance 1. Installation Conditions

To ensure optimum performance and long and stable product life, observe the

following precautions when selecting an installation location.

Note that installing the system in a location that does not meet the requirements

listed below can result in malfunctions and/or system breakdown. Replacement

of components and system repairs for any deterioration in performance or

damage caused by failure to adhere to the following conditions will not be

covered by warranty.

1. Install system in a comparatively dust-free location.

2. Avoid locations subject to corrosive or flammable gases.

3. Avoid locations subject to either strong shocks or continuing low levels of

vibration.

4. Install the system away from direct sunlight or rainwater.

5. Install the system as far away as possible from electrical equipment that

generates strong magnetic fields, electrical fields and high frequencies. Avoid

locations near equipment that generate noise caused by electrical motors and

transformers.

6. Choose a flat location capable of withstanding the design weight of the

system.

7. Do not put it on the slippery seat. Avoid to slip down when the earthquake.

8. The installation environment must be within 15 to 30ºC temperature range and

a 20 to 80% relative humidity range.

9. Avoid locations subject to sharp temperature fluctuations, exhaust from

air-conditioning systems and condensation caused by sharp humidity

fluctuations.

10. Avoid blocking air vents located on the right and left sides, front and rear of the

system.

11. Choose an operating location with good ventilation.

12. Do not operate the system in a cold room.

13. Select a room that can provide sufficient power (frequency, voltage and

current) for system operation.

14. Never use a multiple outlet extension plug.

15. Do not attempt to move the system while holding it by the lid handle or the

control panel, as this can damage to key components. Doing so can also

result in personal injury due to part breakage resulting in the system falling

down.


Rev. A

9

2. Opening the Packaging

This equipment comes packaged in carton, as shown below. The power cord is bundled at

the bottom of the carton box. The gross weight with packaging is 21.5 kg, and the equipment

itself weighs 18 kg. Take sufficient care when removing the equipment from the packaging.

■ Main Unit Package

【Packed Items】

Main Unit

Power Cord

Instruction Manual

Warranty card

Consumable Accessory Pack

1. 48 well snap plate (NJ700) 4 plates

2. Flat cap for snap plate (NJ720) 24 strips

3. 0.2ml Hi-8-Tube (NJ300) 25 strips

4. 0.2ml Hi-8-Flat Cap (NJ302) 25 strips

5. 0.2ml 8-strip tube, individual Flat caps (NJ600) 24 strips

Power Cord

Consumable

Accessory Pack

Inside the upper

tension plate

Instruction Manual

and Warranty card


Rev. A

10

■ Control Computer Package

Notebook PC

AC adapter for PC

USB Cable (supporting USB 2.0)

Thermal Cycler Dice™

Real Time System Software CD for TP700

Thermal Cycler Dice™

Real Time System Driver Software CD

Microsoft® Office Home & Business 2010

Accessory Pack

Note: The contents of the accessory pack may vary.

Check the sama serial Number, TP710 main body and PC label.

same number


Rev. A

11

3. Installation

Use the following procedures when installing system.

1. Confirm that the system power switch is set to off.

2. Connect the system side of the power cord to the power connector on the rear

of the system.

3. Plug the AC power plug of the cord into a power outlet.

The system is designed to operate normally with a 100 to 240 V AC, 50/60Hz

power line.

Make sure that the power outlet is designed for a 3-pronged plug with ground

line.

4. Install the Control Computer in a location near the main unit.

5. Connect the USB cable provided to the connector

on the back of the main unit, and connect the other

end to the USB port on the computer.

Note: Connect the cable to one of all computer USB ports of which the drivers

are installed.

6. After turning the main unit power ON, turn the computer power ON and start

the software. “Instrument: Connected” should be displayed at the bottom right

of the initial screen.

Caution: Connect the AC adapter as a power source for the computer. If

battery power is used alone, experiments may be cut off when

battery power runs out.

7. Please refer to the Software Manual for information about real time PCR and

analysis.

8. When experiments are complete, close the software by File -> Exit.

9. Shut down the Control Computer.

10. Turn the power source for the main unit to the OFF position.

In the interests of safety, and avoiding electric shocks, be sure to connect

the system to a properly grounded power outlet. ！ CAUTION

USB port


Rev. A

12

4. Moving the System

Follow the procedures described below when moving the system to a new location after it has been installed. ● New Location Within the Same Site Follow the precautions listed below when moving the system to a new location

within the same site.

1. Thoroughly clean the system and make sure that there are no tubes or plates remaining in the sample block.

2. Avoid any shock during movement that can affect the operation of this highly sophisticated system.

3. When lifting and carrying the system, be sure to place both hands on each side of the system and lift and carry in a balanced way.

4. Make sure that the new location conforms to the installation requirements described in section “1. Installation Conditions” in Chapter 2.

5. Follow the installation procedures outlined in section “2. Installation” in Chapter 2.

● Moving to a New Site

When moving the system to a new site, clean it carefully and repack it in the packaging in which it was delivered. Note that damage sustained by the system during transport due to improper packaging will not be covered by Warranty even if the Warranty period is still valid. Follow the instructions provided in this chapter when reinstalling the system at the new location.


Rev. A

13

● Chapter 3: Part Names and Functions

Exterior (Lid Closed)

POWER [ON] when the power switch turn ON ( | ).

[OFF] when the power switch turn OFF (○).

(always ON when power is being supplied).

Standby [Flashing] when the main unit is getting ready;

[ON] when ready for operation (a run can be started

immediately using the Run command).

RUN [ON] during program (run) operation;

[OFF] after the operation is complete.

Power ON

Preparing for Operation

POWER: ON, Standby: Flashing, RUN: OFF

Ready for Operation

(Lid temperature stable)

POWER: ON, Standby: ON, RUN: OFF

Run Start

(Program start)

POWER: ON, Standby: OFF, RUN: ON

Returning to Standby

condition

POWER: ON, Standby: Flashing, RUN: OFF

LED display


Rev. A

14

Front (Lid Open)

Explanation of Parts ――――――――――――――――――――――――――――――

① Lid Handle To open the Lid, lift the Lid Handle, slide the Lid towards the

back and push it into the main unit. To close the Lid, pull the Lid

Handle forward and push it down.

② Lid The Lid prevents evaporation by heating the top of the Reaction

Plate. The Lid also allows through path of excitation light and

reflected light.

[Caution]: The equipment becomes very hot inside (108-110°C).

Do not insert fingers.

③ Sample Block Place the Reaction Plate or Tube on the Reaction Block.

[Caution]: Be careful to avoid injuries from burns. The block

heats up to a maximum of 100°C.

④ Lid Lock While the program is in operation, the Lid becomes locked so

that it cannot be opened.

⑤ Ventilation Intake (Front Side)

Openings in the main unit for providing internal cooling by air

ventilation.

[Caution]: Do not cover the Ventilation Intakes.

① Lid Handle

② Lid

④ Lid Lock

⑤ Ventilation Intake

(Front Side)

⑥ Power Switch

ON (｜), OFF (○)

⑦ Ventilation Intake

(Left and Right Sides)

⑧ Lid Lock Catch

③ Sample Block


Rev. A

15

⑥ Power Switch Switch for turning the main unit power to ON (｜) or OFF (○).

⑦ Ventilation Intake (Left and Right Sides)

Openings in the main unit for providing internal cooling by air

ventilation.

[Caution]: Do not cover the Ventilation Intakes.

⑧ Lid Lock Catch Catch for the Lid Lock cylinder.


Rev. A

16

Back

Explanation of Parts ――――――――――――――――――――――――――――――

⑨ Power Cord Connector Connector for Power Cord.

[Caution]: Ensure that you use the Power Cord that

came with the equipment.

⑩ Ventilation Exhaust (Back Side) Openings in the main unit for providing internal cooling.

[Caution]: Do not cover.

⑪ USB Cable Port Connection for the USB cable mini-connector plug.

⑫ Top Cover Cover for the equipment.

[Caution]: Do not open. For service..

⑨ Power Cord Connector

⑩ Ventilation

Exhaust (Back)

⑪ USB Cable Port

⑫ Top Cover


Rev. A

17

● Chapter 4: Explanation of Screens

1. System Startup and Shut Down

1) Startup

① Verify that the Thermal Cycler Dice™

Real Time System Lite main unit is connected

to the computer via the USB cable.

② Turn the Power Switch located at the bottom right of the Thermal Cycler Dice™

Real

Time System Lite main unit to the ON (｜) position.

When the power is turned on in the main unit, The Lid begins to warm up. It takes

about 5 minutes for the Lid plate until its temperature is greater than about 100°C.

The Standby LED blinks until Lid have warmed up sufficiently. When warm-up is

complete and the equipment is ready for use, the Standby LED changes to ON.

③ After the Thermal Cycler Dice™

Real Time System Lite main unit is powered, turn

on the computer.

④ At the Login Screen, input User Name and Password if necessary.

⑤ When the computer has finished starting up, double-click the Thermal Cycler Dice™

Real Time System Software icon on the desktop.

Alternatively, the user may select [All Programs]→[TaKaRa Thermal Cycler Dice]→

[Thermal Cycler Dice Real Time System ] from the Start menu at the bottom left of

the computer screen.

⑥ The splash screen is displayed, and the Thermal Cycler Dice™

Real Time System

Software launches.


Rev. A

18

⑦ The Initial Screen for the Thermal Cycler Dice™

Real Time System Software is

displayed.

Refer to the section on software operation in the Operation Manual for a

description of the software and its method of use.

2) Shut Down

① Select Exit from the File Menu in the Thermal Cycler Dice™

Real Time System

Software.

Caution: If there are experimental files

that have not been saved, a

dialog box will be displayed

asking whether or not the files

should be saved before shutting

down. Save the experiment files

if necessary.

② Select “Turn Off Computer” from the Start menu at the bottom left of the computer

screen.

③ The Shut Down Screen is displayed.

④ Click “Turn Off”. The computer shuts down.

⑤ Turn the Power Switch on the Thermal Cycler Dice™

Real Time System main unit to

the OFF (○) position.


Rev. A

19

2. Initial Screen Menu

The connection status can be verified using the Instrument Status and the Camera Status displays in the Status Bar (bottom of screen). “Connected” is displayed when the computer and any units are connected, and “Disconnected” is displayed if they are not connected, or if power to the main unit is OFF.

Displays connection to computer and main unit.

① ② ③ ④

Temperature:

the current Lid

Temperature.

Lid: Close/Open

Lid Status (Open/Closed)


Rev. A

20

① File Menu

New: Create a new Experiment File

Open/Close: Open experiment files / Close the opened file

Data Manager: Open the Data Manager

Save: Overwrite as the same file name

Save As: Save the experiment file to another file name

Load Template: Load the only format of selected stored experiment

files

Make Full Report: Make a report of experiment file as Word/Power point

data form.

Print: Print out a report of experiment file

Exit: Close the software (program)

② Instrument menu

Run: Start Run/Add Cycle/Pause/Resume/Force Quit

(forcibly terminate)

Filter Set: Settings for Excitation and Detection Filter /

Information for Fluorescent dye / Settings for Display,

Addition, Deletion and Measurement Order

Calibrate: Optical System Calibration Screen

Display Thermal Service Mode for service only

Diagnosis: Self-diagnostics for equipment heating and cooling

systems


Rev. A

21

③ User Menu

Change: Change a user

Setting: Display the User Setting screen

Management: Display the User Management screen

④ Help Menu

Help: Display User’s manual by selected Japanese or

English ( need to install “Adobe Reader”)

About: Display Software version


Rev. A

22

● Chapter 5: Maintenance

1. Self-diagnostic Function

If the PCR reaction does not proceed well, it possible to determine whether the

temperature control for the main equipment is operating normally using the self-diagnostic

function for Sample Block temperature efficiency.

In addition, the status of the temperature control function can be tracked by performing

periodic self-diagnostic tests. There are 3 items in the diagnostic test:

① Rate Test

Tests the heating and cooling rates.

② Cycle Test

Tests the cycle time, overshoot and undershoot .

③ Up-Down Test

Measures the heating and cooling time required to go up 98°C and then down to

10°C.

1) Select [Instrument]→[Diagnosis] from the Initial screen.

Self-diagnostic function

from software Initial Screen

Self-diagnostic function

from after File→Open operation


Rev. A

23

2) The Self-Diagnosis Screen is displayed.

Select the diagnostic items, and click the Start button. The first step of the

diagnostic program starts.

[Note]: If the first phase of the diagnostic program does not change to orange in

the diagram, click the Stop button once and click the Start button again.

① Rate Test Program Execution Screen (Refer to the previous figure.)

Program Contents: 25°C/30 seconds, 94°C/30 seconds, 40°C/30 seconds

Specification:

Maximum heating rate greater than or equal to 3.0°C per second

Maximum cooling rate greater than or equal to 2.4°C per second

1. Check diagnostic items.

2. Click Start

button. 3. It is progressive time.

Results are displayed when self-diagnostics are complete.


Rev. A

24

② Cycle Test Program Execution Screen

Program Contents: 94°C/30 seconds, 55°C/30 seconds, 72°C/30 seconds

Specification:

Overshoot less than or equal to 0.5°C

Undershoot less than or equal to 0.5°C

Length of run time : Within 4 minutes

③ Up-Down Test Program Execution Screen

Program Contents: 98°C/1 minute, 10°C/1 minute

Specification:

Length of run time within 6 minutes


Rev. A

25

2. Optical Calibration

Optical calibration is necessary for calculation of baseline values to ensure that the

obtained 48-well fluorescent display quantities are uniform. When the two

standard-specification wavelength filters are used, use the FAM and ROX calibration plates

which were specially designed for use with the Thermal Cycler Dice™

Real Time System

Lite. It is recommended that calibration be carried out once a year. Check for any soiling or

contamination of the Block before performing optical calibration, and clean if necessary.

Prepare the Test Reagent and the following tools and equipment:

One (1) FAM calibration plate for TP700

One (1) ROX calibration plate for TP700

Cotton swab (for cleaning the Block)

Ethanol (for cleaning the Block)

Plate Centrifuge

Powder-free gloves

1) Cleaning the Sample Block

If the Sample Block is dirty, it may affect the strength of the fluorescence. In order to

check whether the Sample Block is soiled/dirty, tighten the Lid without putting anything

on the Sample Block, and then photograph in FAM and ROX modes and verify that

fluorescence is not visible. If dirty, clean using alcohol and a cotton swab.

Verification of Sample Block Soiling

① Verify that there is no plate on the Sample Block, and close the Lid.

② Open [Instrument]→[Calibrate]→[Optical] from the Initial Screen Menu.

③ Using operation system Windows® 7,

the Use Account Control message appears.

Click “Yes” on the screen.


Rev. A

26

④ Close the initial screen menu and appear again.

In this condition, allow to access the program.

Open again [Instrument]→[Calibrate]→[Optical] from the Initial Screen

Menu.

⑤ Click “Take Picture” in the Optical Calibration Screen.

Set the fluorescent dye to FAM with Expo=200, Gain = 1

in the “Camera _Setting” items.

Set to

Expo [200]

Gain [ 1 ]

Set to “FAM”.

Photo Shoot button

Click


Rev. A

27

⑥ Click the TakePicture button, and a Block Image photograph is taken.

A confirmation message is displayed when photographing is complete.

Click OK.

[Note] Take Picture is started on 103 degree C. of Lid temperature.

⑦ Verify the image.

⑧ If the Sample Block is soiled, soak the cotton swab ethanol and wipe off

any accumulated dirt.


Rev. A

28

⑨ Change to ROX, set Gain to [6] and photograph the Block in the same

way .

⑩ If the Sample Block is soiled, soak the cotton swab in ethanol and wipe

off the accumulated dirt in the same way.

Caution: Be sure to verify the image with both FAM and ROX. Sometimes

soiling is not visible with FAM but is visible with ROX, and vice versa.

Expo [300]

Gain [ 3 ]

ROX

Photo shoot button


Rev. A

29

2) Optical Calibration

Preparation ① The FAM and ROX Calibration Plates each perform 3 cycles of PCR

in the Thermal Cycler Dice™

Real Time System. Melt the calibration

reagent and stir by convection.

PCR program: (95°C/5 seconds, 60°C/30 seconds) x 3 cycles.

② For the 48-well plate, perform the centrifugal process for 3 minutes at

1500 to 1800 rpm to remove Reagent and air bubbles adhering to the

well wall surface. Verify by visual inspection.

Caution: After the centrifuging process, verify that there is no liquid adhering to the

plate wall surface and that no bubbles are present.

If there is still liquid adhering to the walls or bubbles remaining, continue

the centrifugal process until the liquid drops solidly into the tip.

Caution: Wear powder-free gloves for experiments when conducting Optical

Calibration, and be careful not to touch the bottom surface of the plate or

the top surface of the seal.

When the Calibration Plate becomes dirty, Optical Calibration cannot be

performed accurately, and this may affect all test data for experiments

performed afterwards.

Procedure

① Verify that the main unit has completed warming up and the Standby LED is ON.

② Select [Instrument]→[Calibrate]→[Optical] from the Software Screen.

If appear the User Account Control message,

click “Yes”. The initial screen close and open again.

Select [Instrument]→[Calibrate]→[Optical].

○

Liquid level is even

X

Liquid coming into contact with wall surface

X

Contains bubbles


Rev. A

30

③ The Optical Calibration Screen appears.

Verify that only "SearchWellCenter" is at the top

and select FAM from the drop-down menu.

④ Slide the Lid into the main unit and insert the FAM

Plate into the Sample Block.

Caution: Adjust the direction of the plate and insert

securely into the Sample Block.

⑤ Close the Lid securely and wait for 1 minute.

Caution: When the Calibration Plate sheet becomes

cloudy, this influences calibration.

Therefore, when the sheet is heated,

wait approximately 1 minute until the

cloudiness disappears.

Caution: During calibration, the Lid does not lock.

Therefore, be careful not to open the Lid

while calibration is being performed.

⑥ Click the SearchWellCenter button.

⑦ A message is displayed confirming that the Calibration Plate (FAM) is set on the

Sample Block.

Verify that the FAM plate is in place, and click OK.

The right screen is displayed when the

center of each well is detected.

Click OK. The center points of the detected wells are displayed.

③

⑥


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31

⑧ A red spot is displayed in the center of each well. Verify by visual inspection that

this is the center of the Well.

Click the Save button.

Caution: If the red spot is not displayed in the center,

click the SearchWellCenter button again.

Caution: Data is not retained if Save is not selected.

Always be sure to click the Save button.

⑨ When the Save operation is complete, the screen below appears. Click the OK

button.

The diameter of the red circle becomes large, and a confirmation message

is displayed in the center.

⑩ Click the Calibration tab to proceed to Calibration.

Perform Calibration.

Verify that the fluorescent

pigment is FAM, and click

the Calibrate button.


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32

⑪ The Calibration Verification Screen is displayed.

Verify that the Calibration Plate (FAM) is on the Sample Block and click OK.

⑫ FAM calibration starts.

⑬ When FAM calibration ends, a verification screen is displayed. Click OK.

FAM calibration is now complete.

After leaving to cool, open the Lid and remove the FAM Calibration Plates.

Caution: The Calibration Plate becomes hot due to proximity to the Lid heater.

Remove only after it has cooled off.

Next, perform ROX calibration in the same way as FAM calibration only installed

ROX filter.


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33

⑭ Insert the ROX Calibration Plate into the Sample Block and close the Lid.

Click the SearchWellCenter tab A in the area on the left and designate the

fluorescent pigment as ROX in the drop-down menu B . Click the

SearchWellCenter button C , verify that it is the ROX plate, and click OK D

when the confirmation message is displayed.

Caution: Verify that the pigment is ROX in the Fluorescent Pigment Selection

Screen.

Caution: The Lid does not lock during calibration. Therefore, be careful not to

0pen the Lid while calibration is being performed.

⑮ The task of detecting the central position of the wells is complete. Click OK.

A B C D


Rev. A

34

⑯ When the central points of the wells are displayed, verify by visual inspection that

they represent the well centers, and click the Save button.

Caution: If the Save button is not selected, data is not retained.

Always be sure to click Save .

⑰ When saving is complete, the screen below appears. Click OK.

Then click the Calibration tab to proceed to Calibration.

⑱ Select ROX as the fluorescent pigment type from the drop-down menu and click

the Calibrate button.

When the confirmation screen appears, verify that the ROX Plate is set,

and click OK.


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35

⑲ ROX calibration begins.

⑳ When calibration ends, a confirmation screen is displayed. Click OK.

Lastly, remove the ROX Calibration Plate; be sure not to forget this step.

This completes the calibration operation.


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3. Daily Inspections

Perform the following inspections and cleaning periodically in order to maintain the

operational performance of the equipment,

Recommended Once-Weekly Maintenance

Clean the Ventilation Intakes and exhaust (front/back/left/right).

Wipe clean with a cloth, or if there is significant accumulation of dust, clean with a

toothbrush.

* The main equipment, including the Sample Block, LED’s light source and

Electronic circuits, need to be cooled by air. Dirty ventilation intakes may cause

decreased functional capability and shortening of operating lifetime.

Clean the Lid and the main unit.

Wipe the outside of the equipment with a cloth soaked a little in a neutral detergent.

Then wipe with a dry cloth.

Verify the sliding action of the Lid. Wipe off any dirt or debris around the rails.

* Wiping the Lid rails with a dry cloth is sufficient. It is not necessary to lubricate.

Disinfect by wiping with a 10% bleach solution. Allow to leave for 10 minutes, then

wipe with a 70% alcohol solution in the same place. Lastly, wipe with a dry cloth to

dry the surface.

Caution: If bleach solution remains on the equipment, it may lead to corrosion.

Therefore, always wipe with alcohol after using bleach.

Recommended Once-Monthly Maintenance (or occasionally based on frequency of

use or soiling conditions):

Clean the Sample Block.

* For the cleaning method, refer to 3. Optical Calibration 1) Sample Block Cleaning

in this chapter.

Once Yearly

Optical Calibration is recommended.


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● Chapter 6: Troubleshooting

Symptom Cause Correspondence

Nothing happens when the

Power Switch is turned ON.

Power is not being supplied. Verify the Power Cord connection on

the main unit side and the power

outlet side.

The Power Cord is not securely

plugged in to the power outlet or

the main unit connector.

The power outlet is not providing

power.

Check the power source equipment.

Thermal protector worked on

overheat of the sample block.

Wait the moment that the sample

block cooled.

Thermal fuse on the cooling fin

was melt down.

Need to replace it. Make a request

for repair.

Trouble occurred on electronic

circuits.

The Power Switch remains

OFF even when it is turned

on.

Malfunction of internal electrical

circuits in the main unit.

(The Power Switch works as the

over-current breaker.)

Immediately unplug the Power

Cable and make a request for repair.

Power Switch malfunction. Replace Power Switch.

It occur unusually when the

power switch turned on.

Optical filter changer is not

stopped on home position.

You hear a strange noise.

Turn off the power as soon as

possible and make a request for

repair.

The Lid does not close. There is an obstruction between

the Lid Heater and Sample Block.

Remove the obstruction.

Sample plate or tubes do not

seated correctly into the well.

Re-seat them correctly.

The Lock function of the Lid is

broken.

Disassemble the Lid and make a

request for repair.

Software do not launch. Software is not installed correctly. Delete the software and re-install it.

Not installed .NET Framework 2.0

or later version.

Install .NET Framework 2.0 or later

version.


Rev. A

38


The program does not

start.

The main unit is not connected to

the PC by USB cable.

Connect the USB cable.

No communication between the

main unit and the PC.

Refer to the last symptom.

[ Instrument “Disconnected” and/or

Camera “Disconnected”].

Open the experiment file that is

not a new experiment file.

Create a new experiment file.

Warm-up for the Lid Heater has

not yet finished to warm up.

Try again after allowing 5 minutes

for the equipment to warm up.

The heater or a safety fuse has

burned out.

A part needs to be replaced. Make a

request for repair.

When starting the program,

the message “Please put

the lid on” is displayed.

The Lid is not completely closed. Close the Lid completely.

The Lid switch is broken. The switch must be replaced or

adjusted. Make a request for repair.

The program cycle stops

before completion.

The stepping motor has

malfunctioned.

The part needs to be replaced and

calibrated. Make a request for

repair.

The position-detecting sensor has

malfunctioned.

Make a request for repair.

Under PC Power Settings, any

settings are OFF.

Monitor/Hard Disk power is always

ON. Change to “OFF”.

Sleep setting is some hours.

Change to “Never” under “sleep

after”.

Under Screen Saver Settings on

PC, its setting is not OFF.

Change to “OFF” .

Thermal protector switch on the

Sample block heat sink works for

over temperature.

The cycler is too hot. Set the

environmental operating

temperature to 20-23℃. Make

sure the Ventilation. If the protector

or a fan is broken, need to replace.

Make s request for repair.


Rev. A

39

Symptoms Cause Correspondence

The program cycle stops

before completion.

When the PC was started up, the

camera or/and the USB-serial

bridge driver was not recognized.

On the start screen, display the

message under bar.

[Camera: Disconnected]

Refer to 1,2,3

[Instrument: Disconnected]

Refer to 1,2,4

Refer to the section on Computer

settings in Appendix .

1. Shut down the system. After

turning the power ON for the main

unit, turn the power ON for the PC.

2. Alternatively, leaving the power

ON for both the PC and the main

unit, unplug the USB cable, plug it in

again, and wait 1 minute.

3. The method for verifying the

camera drivers is to select [Imaging

device] under [Start]→[Control

Panel]→[System]→[Device

Manager]. Check to see if the device

is recognized.

4. Under the device manager on the

PC, make sure “COM1” of Ports /

ATEN USB to Serial Bridge (COMx).

On the control PC, work on

another operation except unit

control.

Operate the system only.

PC had too loaded and then

stopped.

The fluorescence

measurement value

(graph) is abnormal.

< Graph is too low around

baseline.>

The Test Reagent Plate is not in

place.

Set the Test Reagent Plate.

LED can not light. LED or a power supply is broken.


The camera has malfunctioned. The part needs to be replaced.


The fluorescence

measurement value

(graph) is abnormal.

< Graph is wavy.>

The Filter Unit is no correctly set. Correctly set the Filter Unit.

Filter unit exchanger is no move

smoothly.

Need it to replace or repair.


LED or power supply for LED is

malfunction.

make a request for repair.


Rev. A

40


The rate of temperature

heating or cooling is too

low.

The environmental operating

temperature being used is not

appropriate.

Set the environmental operating

temperature to 20-23°C and perform

the self-diagnostic test. If out of

range, make a request for repair.

The Peltier element is broken. The part needs to be replaced.


Intake and exhaust are close to

the walls.

Make sure that ventilation is good.

Cooling fan or Exhaust fan is

broken.

Need to replace it. Make a request

for service.

The computer froze. CPU confused. Re-start the computer. Running data

is saved until PC froze.

PC can not communicate to

the main unit.

On the start screen, display

the followings under status

bar.

Instrument “Disconnected”

and/or

Camera ”Disconnected”

Power switch is OFF position. Turn the switch to ON.

The camera driver or/and the

USB-serial bridge driver was not

recognized.

1. Shut down the system. After

turning the power ON for the main

unit, turn the power ON for the PC.

2. Alternatively, leaving the power

ON for both the PC and the main

unit, unplug the USB cable, plug it in

again, and wait 1 minute.

Connected another USB terminal

on the PC for moving the system.

Connect the same USB port of start

setting system.

Under the device manager on the

PC ,verify “COM1” of Ports/ ATEN

USB to Serial Bridge(COM x).,

Change to “COM1” if not.

The camera driver was not

recognized.

The method for verifying the camera

drivers is to select [Imaging device]

under [Start]→[Control Panel]→

[System]→[Device Manager].

Check to see if the device is

recognized.

Port setting of using PC changed

automatically. for example case,

Insert another USB port or

connect a USB device to

recognized USB port.

Verify the driver settings as follows;

Start>Control Panel>Hard>Device

Manager/ Port ATEN USB=COM1,

and Imaging devices =Lumenera

(refer to Appendix 1)


Rev. A

41

Error code

Display the following error when the system catches error. For recover the system shut

down and restart. If it will not clear, please make a request for repair.

Error code Cause

11 Indicates disconnected center sensor on the sample block.

12 Indicates short in center sensor on the sample block.

13 Indicates disconnected left sensor on the sample block.

14 Indicates short in right sensor on the sample block.

15 Indicates disconnected right sensor on the sample block.

16 Indicates short in right sensor on the sample block.

17 Indicates disconnected lid sensor.

18 Indicates short in lid sensor.

19 Indicates disconnected cooling plate sensor on the sample block.

20 Indicates short in cooling plate sensor on the sample block.

21 Indicates disconnected circuit of the cooling fan.

22 Indicates short in circuit of the cooling fan.

23 Indicates lid temperature control.

24 Indicates block temperature control error.

26 Indicates home position error.


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● Chapter 7: Specifications

Main Unit Basic Specifications

Product Name Thermal Cycler Dice™ Real Time System Litet

Part Number TP710

Objects Processed 48-Well Plate Cap Seal Type

8 Series Tube Cap Seal Type

Optical Detection

System

CCD CAMERA (1280 x 960 resolution).

Computed for each well based on the image data.

Optical Filter Filter Unit (Include excitation/emission filter and dichroic mirror)

2 Filter Units as standard:

FAM (SYBR Green I ), ROX (Texas Red)

Maximum of 4 can be installed: FAM, ROX, Cy5, HEX/VIC

Light Source LED x 3 (Blue LED x 1, White LED x2 )

recommended total usage time: 50,000 hours

Temperature Control

Method

PID Control (Temperature Sensor Pt 1000Ω)

Heating and Cooling

System

Peltier Element

Cooling Capability 10°C (reaction block temperature when the environment

temperature is 30°C)

Temperature Range 10.0 - 99.9°C (increments of 0.1°C)

Temperature Display

Information Display

Sample temperature calculated from block temperature; display

sent to control PC (increments of 0.1°C)

Main Unit Display LED display: POWER / Standby / RUN

Rate of Temperature

Increase/Decrease

Maximum 3.0°C / second temperature heating rate

Maximum 2.4°C / second temperature cooling rate

(with an environment temperature of 20°C).

Run Modes Fast mode, Normal mode

Lid Holding

Temperature

When set program temperature to 20°C or more:

Lid temperature at about 108°C.

When set program temperature to less than 20°C:

Lid temperature at about 40°C.

Lid Lock Function Locks the Lid with an electromagnetic lock while running,

and releases the lock when all processes are complete.

Operating the Pause or Stop functions during a run releases

the lock. When a paused run is restarted, the lid is locked again

and the run continues.


Rev. A

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Optical Calibration

Function

For use with Takara Thermal Cycler Dice Real Time System Lite

FAM/ROX Calibration Plate. (FAM/ROX).

Self-diagnostic

Function

Various sensors for broken wires, short circuits, fan lock, and

Block Temperature, Lid Temperature, or Heating/Cooling Rate

abnormalities

Safety Devises Lid Heater : Thermo Fuse ( 169 degree C)

Heat Sink of the Sample Block : Thermal Guard (85 degree C)

+ Thermo Fuse (113 degree C)

Main Switch : Over Current Switch (10 A)

Timer Battery Lithium Cell, Type CR2032 3V

Connection with PC USB 1 port (COM1 Port use)

Supply Voltage AC 100-240 V 50/60 Hz

Maximum Current 3.7 A (at 100 V)

Power Cord 125 V 15 A cable (3 m) including a 3-pin plug connection with a

ground pin (commonly used in Japan and North America)

Exterior Dimensions

/ Weight

Width 294 × Depth 480 × Height 304mm

18 kg for main unit only. (21.5 kg including packaging.)


Rev. A

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Basic Specifications of Computer

Product Name Computer to be used with TaKaRa Thermal Cycler Dice™ Real Time

System

Part Number TP820

PC Model (ex.) Fujitsu LIFE BOOK A561/D

Dimensions /

Weight

374 (Width) × 245 (Depth) × 35 (Height) mm

2.4 kg

Specifications OS: Microsoft® Windows

® 7 Professional Service Pack 1

32bit (English / Japanese)

Intel® Core™ i 5-2520M Processor (2.50GHz)

Memory: 2 GB

Hard Disk: 250 GB

LCD Size: 15.6 inch HD(1366x768) LED back light LCD display

Optical Drive: DVD super multi drive

USB port x 4 USB version 2.0

Standard

Accessory

USB mouse, USB Memory key

Microsoft® Office Home & Business 2010 (English or Japanese):

include Word 2010/ Excel® 2010/ PowerPoint

® 2010/ Outlook

® 2010

/ OneNote®2010

Note: The computer specifications may change according to market

conditions. This specification is at the time of June, 2012.

Application Software Basic Specifications

Product Name Thermal Cycler Dice™ Real Time Software

Part Number TP730

Media One (1) Program CD and One(1) Driver software CD

For OS Windows® XP / Vista / 7 32bit / 64bit *

* This system driver are installed 32bit version for the Camera and the USB

Converter. The software can use on another computer that is installed the 64 bit

OS for analysis only.


Rev. A

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● Chapter 8: Consumption

■ 48 well plate

Code Product Quantity Comments

NJ700 48 well Snap Plate 20 plates

NJ720 Flat Cap for Snap Plate 120 seats Cap for NJ700

■ Strip Tubes


NJ300 0.2ml Hi-8-Tube 125 strips Strip of 8 attached caps for use

with 0.2 mL strip tubes

NJ302 0.2ml Hi-8-Flat Cap 125 strips Flat caps for easy closure and

sample labeling

■ Strip Tube with individual Flat Cap


NJ600 0.2ml 8-Strip Tube, individual Flat

Caps

120 strips Strip of 8 tubes with each cap.

Can use one tube by separation.

■ Option Filter Units（TP900/960, TP800/860 Multi Wave Model）


TP702 Filter Unit HEX for TP700

(For HEX / VIC)

1 unit Only Factory setting

TP703 Filter Unit Cy5 for TP700

(For Cy5)

1 unit Only Factory setting


Rev. A

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Appendix

Thermal Cycler DiceTM

Real Time System TP700/760

Computer settings

☆☆ Windows 7 ☆☆

A. Computer Driver Setup - - - - - 47 B. Software Install / Uninstall - - - - - 52 C. Computer Basic Setup - - - - - 57

TP700/760 Instrument Manual Windows7

Rev. A

47


A．Computer Driver Setup

Computer Driver Settings

① Search the “flycapture 2.2.3.14_x86.exe” from the Thermal Cycler Dice™ Real Time

System Driver Software CD and double-click to launch it.

On the way, when the User Account

Control dialog appear, click on Yes .

② Appear the Setup Wizard, Click the

Next button.

③ Check the accept the terms in the

License Agreement to agree to the

License Agreement and

click on Next .

④ Click on Next .

FlyCapture2 Installer

CD/DVD drive


Rev. A

48

⑤ Input your name and organization if

need, select “Anyone who uses this

computer” and release the check of

“internet”.

Click on Next .

⑥ Click on Next again.

⑦ In the next screen, select “Complete” .

Then click on Next .

⑧ Check two items of USB and release

the check mark of “Install UsbPRO....”.

Click on Next .

Check on

□Install UsbPRO.. remove the check

check

In the next screen, check “Click

confirm” and then click on Next .

Remove the check.


Rev. A

49

⑨ Confirm the check “The installer will disable ....” , and click on Next .

Then click on Install .

⑩ Start to install and finish the wizard of

camera driver. Click on Finish .

⑪ The restarts windows appear, click on Yes for restart.

⑫ Open the device manager.

Show the “PGR Chameleon CMLN-13S2M”.


Rev. A

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⑬ Install a USB – Serial Bridge driver.

Double-click on the ATEN “cvrs9_1.0.075.exe” file inside the Driver CD.

⑭ Right-click on “Computer” icon on the

desktop ( or Start > Computer ) and select

Properties from the menu. Open the

“Device Manager”.

⑮ Under Ports (COM & LPT), set “ATEN

USB to Serial Bridge” (COMx) to COM1.

If OK, go to the item ⑲.

⑯ To change the settings, right-click on

“ATEN USB to Serial Bridge (COM x) ”

and open “Properties” from the menu.

⑰ In the Properties box, under Port

Settings, click on Advanced .

Caution: If “ATEN USB to Serial Bridge”

is not set to COM1, then change to “COM1”

as the following procedures.


Rev. A

51

⑱ Set COM Port Number to COM1

and click OK.

⑲ Close the Device Manager, and open it again to verify that the settings are correct.

Under Ports (COM & LPT) Settings, verify that the “ATEN USB to Serial Bridge

(COM1)” setting is in effect. For other devices, verify that “?” is not displayed. If a

question mark is displayed, double-click on that device, open Properties, and click

Reinstall Driver. The driver is then reinstalled.

⑳ If the computer has multiple USB ports, set up the driver according to the

procedure described above after changing the USB cable connection. Also, make

sure that the port setting is COM1.

Caution: Make sure to connect the main unit to the USB port on the computer that

has been set. If there are multiple ports and all of them are set in the same

way, then every time the computer is used, the connected USB cable will

be automatically detected. If this setting has not been performed, the Install

Shield Wizard appears and the driver settings are requested. Please set

the driver settings if they have not already been carried out.

21. Eject the CD and reboot the computer.

TP700･760 Instrument Manual Windows 7

Rev. A

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B．Software Install / Uninstall

If the Thermal Cycler Dice Real Time System Software is being installed for the

first time, perform the steps described in section 1 (Install).

If the software version is being upgraded, first perform the steps in section 2

(Uninstall), and then start the procedure from section 1.

1. Install

① Insert the Thermal Cycler Dice Real Time System Software CD in to the CD drive.

② Make sure the OS is Windows 7

English version. Double-click on

the English folder in the CD drive.

③ Double-click on the setup.exe file

inside the open folder to launch the

installation program.

④ In the initial screen, click the Next

button.


Rev. A

53

⑤ Click the Next button in the screen

that is displayed below.

○ The installation folder can be

changed, but please use the

default folder unless there are

special circumstances.

○ Use default (“Just me”) for the

user settings.

⑥ Click the Next button in the screen

below. The installation process

begins.

⑦ The Installation Execution screen

is displayed.

On the way, when

“User Account Control”

appear ,click on Yes .

⑧ When installation is complete, the right screen is displayed. Click “Close” button.

And then The restarts window appear, click on “Yes”.

CD/DVD drive

RP1_RealTimePCR_Res.exe


Rev. A

54

2. Uninstall

① From the Start menu, go to Control Panel and double-click on the Programs icon.

② When the “Uninstall or Change a Program” window is displayed, select “Thermal

Cycler Dice Real Time System Software ” from the Currently Installed Programs list.

③ Click the Uninstall button.

When the confirmation dialog box is displayed, click Yes.

View by Category View by Small icons


Rev. A

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④ The uninstall operation starts.

On the way, when the User Account

Control dialog

appear, click on “Yes”.

⑤ When complete, a dialog box inquiring whether to restart now or later is displayed.

If additional software is to be installed, select “No”. The following screen is

displayed. After verifying that the Thermal Cycler Dice Real Time System program

has been removed, click Close. Then proceed to the installation steps detailed in

section 1.Alternatively, click Yes to restart the computer and continue settings.

RP1_RealTimePCR_Res.exe

TP700/760 Instrument Manual Windows 7

Rev. A

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C. Computer Basic Setup

Computer Settings

① In the Start menu, right-click on

Computer.

Click “Show on Desktop” to display

the Computer icon on the desktop.

This icon can then be selected when

needed for setting purposes.

② Right-click on the screen and open

“Personalize”. Under the Screen

Saver tab, set “None” for Screen

Saver and click on “Apply” .

Then, click on the change power

settings


Rev. A

57

③ Under Power Options, select “High performance” and “Change plan settings”.

If “High performance” is not appeared, click on the arrow (Show additional plans).

In the Edit Plan Settings, set as shown below and click on the Save changes.

④ In the same way, Power Options >

High performance > Change plan

settings > Change advanced

power settings. Set as follows.

Dim the display

Turn off the display all “Never”

Put the computer to sleep

Click on the Save changes.


Rev. A

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⑤ Right-click on the Computer icon

and click Properties to enter the

System Properties window.

Click on “Advanced system

settings” to enter the system

properties, click Advanced tab,

then “Settings...”.

⑥ Under the Visual Effects tab,

remove the checks from the

following two boxes and click OK.

→ □ Save taskbar thumbnail

previews

→ □ Show windows contents

while dragging

⑦ Ensure that file extensions are

displayed. Under [Start]→[Control

Panel (view by Large icon or Small

icon)] →[Folder Options], click the

View tab. Under Advanced Settings,

remove the check mark beside

“Hide extensions for known file

types”. Click on “OK” button.


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⑧ Hardware Verification

Right-click on the Computer

icon on the desktop and select

[Properties]→[Device Manager].

Verify that the following devices

are installed normally. If a

question mark is shown for an

item, right-click on that icon and

select [Properties]→ [Re-install]

to re-install the drivers.

・ Imaging devices: Lumenera

USB 2.0 Camera is normal.

・ Ports (COM & LPT) is normal

and COM 1 is selected.

・ All the USB drivers are

normal.

2012.06.08

TAKARA BIO INC.

SETA3-4-1, OTSU, SHIGA, 520-2193

URL: http://www.takara-bio.com

TP900 v4.01E Instrument Manual Manual/2... · 2020-01-16 · 1 Precautions for Using TaKaRa...

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Transcript of TP900 v4.01E Instrument Manual Manual/2... · 2020-01-16 · 1 Precautions for Using TaKaRa...