TOYOPEARL GigaCap® DEAE-650M

A new member of the renowned TOYOPEARL GigaCap Series

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TOYOPEARL GigaCap ® Series

Full line-up of high capacity ion-exchange media

Anion-exchanger

•TOYOPEARL GigaCap Q-650M

•TOYOPEARL GigaCap DEAE-650M

Cation-exchanger

•TOYOPEARL GigaCap S-650M

•TOYOPEARL GigaCap CM-650M

Mixed-mode resin (CIEC type)

•TOYOPEARL MX-Trp-650M

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TOYOPEARL GigaCap DEAE-650M

Key features:

• High dynamic binding capacity

• DEAE-type weak anion-exchanger (AEX)

Increase productivity through transfer of traditional DEAE-type

AEX to TOYOPEARL GigaCap DEAE-650M

• Example: Factor VIII, insulin,

• TOYOPEARL GigaCap DEAE-650M has slightly stronger retention than

traditional TOYOPEARL DEAE-650M

Benefit of traditional TOYOPEARL DEAE-650S/M/C

• Sharper peaks at low salt concentration

• Larger effective pore size support separation of huge biomolecules

• Three different particle sizes (S, M & C grades) available

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Dynamic binding capacities were determined at 10% breakthrough

Column size: 6 mm ID x 4 cm

Loading buffer: 0.05 mol/L Tris-HCl buffer (pH 8.5)

Elution buffer: 0.05 mol/L Tris-HCl buffer (pH 8.5) + 0.5 mol/L NaCl

Linear velocity: 212 cm/h

Detection: UV @ 280 nm

Sample: BSA (1 g/L)

SBC Conditions:

Adsorption buffer 0.05 mol/L Tris-HCl buffer (pH8.5)

Protein concentration 10 g/L, Total protein; BSA 210 g/L-gel

TOYOPEARL GigaCap DEAE-650M BSA DBC exceeds all commercial DEAE media

Comparison of DEAE-type Anion-Exchangers

Particle size Ion-exchange Recovery (%)(um) capacity (eq/L) Static Dynamic （DBC）

TOYOPEARL GigaCap DEAE-650M 50-100 0.23 179 165 100TOYOPEARL GigaCap Q-650M 50-100 0.17 191 172 97TOYOPEARL DEAE-650M 40-90 0.11 30 25 97Capto DEAE 90 (median) 0.29-0.35 143 140 96Fractogel EMD DEAE(M) 40-90 - 52 - -

ProductBinding capacity (g/L)

Crosslinked Agarose DEAE

Tentacle type DEAE

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Dynamic binding capacities were determined at 10 % breakthrough

Column: GigaCap DEAE-650M 6 mm ID x 4 cm

Loading buffer: 0.05 mol/L Tris-HCl buffer (pH 8.5) , Flow rate: 212, 407, 623, 739 cm/hr

Elution buffer: 0.05 mol/L Tris-HCl buffer (pH8.5) + 0.5 mol/L sodium chloride

Detection: UV @ 280 nm

Sample: BSA (1 g/L)

TOYOPEARL GigaCap DEAE-650M has high binding capacity even at high flow rate

Dynamic Binding Capacity @ Various Flow

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CIP conditions

Alkaline washing solution: 0.5 mol/L NaOH

Buffer washing solution: 0.05 mol/L Tris-HCl (pH8.5) + 0.5 mol/L NaCl, Washing flow rate : 0.5 mL/min.

Alkaline washing column volume: 27 CV / cycle, washing time : 1 hr

Buffer washing column volume: 10 CV / cycle

DBC conditions

Column size : 6 mm ID X 4 cm

Binding buffer : 0.05 mol/L Tris-HCl (pH 8.5)

Sample : BSA (1 g/L), Loading flow rate : 1 mL/min. Detection : UV @ 280 nm

TOYOPEARL GigaCap DEAE-650M shows high chemical stability

GigaCap DEAE CIP Study with 0.5 M NaOH

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Column size: 7.5 mm ID x 7.5 cm

Buffer A: 0.05 mol/L Tris-HCl (pH 8.5); Buffer B: 0.05 mol/L Tris-HCl + 1.0 mol/L NaCl (pH 8.5)

Gradient: 120-min linear gradient from 0 to 100% B

Flow rate: 1.0 mL/min, Detection: UV @ 280nm

Sample:1. transferrin (2.9 g/L), 2. ovalbumin (6.5 g/L), 3. trypsin inhibitor (10.0 g/L)

Injection volume: 100 µL

Selectivity of High Capacity DEAE-type AEX (1)

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1

3

Agarose based DEAE

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Column size: 7.5 mm ID x 7.5 cm

Buffer A: 0.05 mol/L Tris-HCl (pH 8.5); Buffer B: 0.05 mol/L Tris-HCl + 1.0 mol/L NaCl (pH 8.5)

Gradient: 120-min linear gradient from 0% to 100% B

Flow rate: 1.0 mL/min, Detection: UV@280nm

Sample:1. transferrin (2.9 g/L), 2. ovalbumin (6.5 g/L), 3. trypsin inhibitor (10.0 g/L)

Injection volume: 100 µL

Selectivity of High Capacity DEAE-type AEX (2)

TOYOPEARL DEAE-650M shows sharpest peaks at low salt concentration

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1

3

Agarose based DEAE

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Mechanical Stability of GigaCap DEAE-650M

Column size: 22 mm ID x 20 cm

Eluent : 0.1 mol/L NaCl

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0.05

0.10

0.15

0.20

0.25

0.30

0 200 400 600 800 1000 1200 1400 1600

Colu

mn p

ress

ure

(M

Pa)

Linear Velocity （cm/hr）

Brand A (agarose, DEAE)

TOYOPEARL GigaCapDEAE-650M

Crosslinked Agarose DEAE

TOYOPEARL Anion-Exchanger Map

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Selection of TOYOPEARL AEX Resins

Initial recommendation →

Resin screening →

Large volume sample →

Salt tolerant →

Low salt feedstock →

- Sharper peaks →

Antibody FT mode →

Factor VIII purification →

Acidic polysaccharide →

Oligonucleotides →

Antibiotics, small MW API →

Strong alkaline CIP (1 M NaOH) →

GigaCap series

ToyoScreen IEC-A, TS RoboColumns

GigaCap series

Q-600C AR

GigaCap series, SuperQ-650

DEAE-650,

GigaCap Q-650M/Q-600C AR

DEAE-650, GigaCap DEAE-650M

GigaCap Q-650M

TSKgel SuperQ-5PW(20)

GigaCap Q-650M, SuperQ-650

Q-600C AR

Ordering Information

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Product P/N Volume

TOYOPEARL GigaCap DEAE-650M 22865 100 mL

TOYOPEARL GigaCap DEAE-650M 22866 250 mL

TOYOPEARL GigaCap DEAE-650M 22867 1000 mL

ToyoScreen GigaCap DEAE-650M 22872 1 mL x 6

ToyoScreen GigaCap DEAE-650M 22873 5 mL x 6