Toxicology Final Presentation Dyad 1 Hassanain A Sola A About Caffeine Stimulant of the Central Nervous System Increases Heart Rate Acts like Adrenalineports/ caffeine.1.jpg Problem & Hypothesis How does caffeine affect the development of Danio rerio embryos? The caffeine will almost double the heart rate of the fish as well as cause abnormalities in the structure of the Fry. Materials 80 Zebrafish Embryos Pipettes Starbucks Coffee (Fill in the kind and size) Distilled Water Graduated Cylinder 16 Petri Dishes Microscope Stopwatch Journal Methyl Blue Incubatorteachers.thelanguagemenu.com/data/uploaded/ma taerial.jpg Procedure: Preparing the Solutions 1.Gather materials 2.Fill 1 Petri Dish with 19 mL of distilled water and 1 mL of coffee. 3.Fill 1 Petri Dish with 18 mL of distilled water and 2 mL of coffee 4.Fill 1 Petri Dish with 17 mL of distilled water and 3 mL of coffee. 5.Fill 12 Petri dish with 20 mL of distilled water and 3 drops of Methyl blue /PetriDish%206104_M.jpg Procedure: Creating The Experimental Group 1.Using a pipette, carefully extract 5 eggs and place them in the 5% solution. 2.Place 5 more eggs into the 10% solution. 3.Place 5 eggs in the 15% solution. 4.Place 5 eggs in the control solution 5.Using the stopwatch, time 5 minutes. 6.Remove eggs from each experimental solution using the pipette and place them in a control solution. Procedure: Observing The Embryos 1.Extract the embryos onto empty Petri dish using the pipette. 2.Place the Petri dish under the microscope. 3.Look for any abnormalities as well as checking the amount living in each group. 4.Check the heart beat of each embryo and take the average based on the coffee concentration. 5.Record data in journal.network.com/direct/dbimage/ /Microscope.jpg Procedure: Wrapping Up 1.Overnight, place the embryos in the incubator at a temperature of 27 C 2.Continue previous steps for 2 more trials. Results Trial 1 (Table) Day 1Day 2Day 3Day 6 Control4310 5% % %5100 Results Trail 1 (Graph) Results Trial 2 (Table) Day 1Day 2Day 3Day 4 Control10 8 5% % %7422 Results Trail 2 (Graph) Results Trial 3 (Table) Day 1Day 2Day 3Day 4 Control % % %7322 Results Trial 3 (Graph) Final Observations The caffeinated embryos had smaller yolk sacks than the control group. (Yolk Sacks) Short, thin, and curved in experimental group. Long and straight control (Body Type) Experimental group developed faster than control fries (Development Rate) The average heart rate for the 5% was 186 BPM, for 10% 222 BPM, and for 15% 242 BPM. The control had 120 BPM (Heart Rate) Body Shape 15% Solution Control Group Rapid Development (~3 Days) 10% Solution Control Group Error Analysis Methyl Blue. It was not used in the first trial until the second day. Pipette. Some of the embryos may have been lost while transferring with the pipette. Temperature. The incubator was too hot on some days. Some days the embryos/fries were not placed in the incubator.