Top producing beamlines of the world
description
Transcript of Top producing beamlines of the world
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Top producing beamlines of the world
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http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html
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Top producing beamlines of the world
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http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html
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http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html
http://biosync.sdsc.edu/
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Number Description Percent
446028 Images (~7 TB) 33%
2346 Data sets 47%
449 MAD/SAD (1:2) 19%
48 Published 2%
8.3.1 in 2003
Beamline Efficiency
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Seconds Description Percent
104490 Assigned and available 91%
42093 Shutter open 40%
52684 Collecting (3026 images) 50%
51806 Something else 50%
Beamline Efficiency“representative” 8.3.1 user
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Seconds Description Percent
51806 Something else 100%
247s 45
Mounting 22%
229s 37
Centering 16%
179s109
Strategizing 38%
309s 37
Prepping 24%
Beamline Efficiency“representative” 8.3.1 user
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Seconds Description Percent
16820 Something else 100%
10s 45 Mounting 3%
30s 37 Centering 7%
140s109
Strategizing 90%
0s 37 Prepping 0%
Beamline Efficiency“expert” user
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Touch screen
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Traffic light
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firewire
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DVD data archive
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Beamline pager
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Ice-B-gone
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Foam dewars
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Foam dewars
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Zero-parallax optics
pinhole
prism
microscope
backstop
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Zero-parallax optics
pinhole
prism
microscope
backstop
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Zero-parallax optics
pinhole
prism
microscope
Styrofoam™ backlight
backstop
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Zero-parallax optics
pinhole
prism
microscope
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Apr 6 – 24 at ALS 8.3.1
Elven Automation
27,686 images collected
148 datasets (15 MAD)
31 investigators
56 unique cells
5 KDa – 23 MDa asymmetric unit
0.94 – 32 Å resolution (3.2 Å)
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Apr 6 – 24 at ALS 8.3.1
Elven Automation
148 datasets
117 succeded
~3.5 (0.1-75) hours
31 failed
~61 (0-231) hours
2 / 15 MAD structures
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Number Description Percent
446028 Images (~7 TB) 33%
2346 Data sets 47%
449 MAD/SAD (1:2) 19%
48 Published 2%
8.3.1 in 2003
Beamline Efficiency
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Hampton Pin
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Syrrx Pin
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plastic Pin
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Yale Pin
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SuperPin
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SuperTongSuper Tong
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Hampton PinSuper Tong
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Syrrx PinSuper Tong
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plastic PinSuper Tong
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Yale PinSuper Tong
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SuperTong
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thaw
Radiation Damage
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Incremental strategy
incremental_strategy.com merged.mtz auto.mat
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Incremental strategy
incremental_strategy.com merged.mtz auto.mat
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OTIS
shoot nextsample
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OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
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OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
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OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
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OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
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OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
![Page 48: Top producing beamlines of the world](https://reader035.fdocuments.net/reader035/viewer/2022062723/56813ef0550346895da96b25/html5/thumbnails/48.jpg)
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
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OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
![Page 50: Top producing beamlines of the world](https://reader035.fdocuments.net/reader035/viewer/2022062723/56813ef0550346895da96b25/html5/thumbnails/50.jpg)
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
![Page 51: Top producing beamlines of the world](https://reader035.fdocuments.net/reader035/viewer/2022062723/56813ef0550346895da96b25/html5/thumbnails/51.jpg)
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
![Page 52: Top producing beamlines of the world](https://reader035.fdocuments.net/reader035/viewer/2022062723/56813ef0550346895da96b25/html5/thumbnails/52.jpg)
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
![Page 53: Top producing beamlines of the world](https://reader035.fdocuments.net/reader035/viewer/2022062723/56813ef0550346895da96b25/html5/thumbnails/53.jpg)
OTIS
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
![Page 54: Top producing beamlines of the world](https://reader035.fdocuments.net/reader035/viewer/2022062723/56813ef0550346895da96b25/html5/thumbnails/54.jpg)
OTIS
recentered data set from 50º to 150º with 1º oscillations using 1 Å x-rays detector at 2.5 Å resolution
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