Tokyo Medical and Dental University, Tokyo, Japan, 2000-2005.
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Transcript of Tokyo Medical and Dental University, Tokyo, Japan, 2000-2005.
Tokyo Medical and Dental University , Tokyo, Japan, 2000-2005
Ginsenoside Re, a Main Phytosterol of Panax ginseng,Activates Cardiac Potassium Channels via a Nongenomic
Pathway of Sex Hormones
Chang-Xi Bai, Asami Kaihara, Eri Ozaki, Takashi Kawano,Yutaka Nakaya, Muhammad Awais, Moritoshi Sato, Yoshio Umezawa, and
Junko Kurokawa
Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University
MOLECULAR PHARMACOLOGY Vol. 70, No. 6
Copyright © 2006 The American Society for Pharmacology and Experimental Therapeutics
28134/3156556
Mol Pharmacol 70:1916–1924, 2006
Both panaxadiols and panaxatriols activate IKs.
NO produced by eNOS, but not nNOS, is responsible for IKsenhancement by ginsenoside Re.
Signaling cascade of IKs enhancement by ginsenoside Re. A–C,effects of SH-6 (A), an Akt inhibitor, PP2 (B), a c-Src inhibitor, and
wortmannin (C), a PI-3 kinase inhibitor, on ginsenoside Re-induced IKsenhancement.
Effects of preincubation with various blockers on ginsenoside Re (3 M)-induced IKs enhancement
Involvement of sex hormone receptors on IKs enhancement by ginsenosides
Effects of ginsenoside Re on Akt phosphorylation
Ginsenoside Re binds to the human AR, ER, and PR
Effects of ginsenosides on proliferation of MCF-7 and LNCaP
Effects of ginsenoside on recruitmentof coactivator examined by
FRET experiments
Nontranscriptional Regulation of Cardiac Repolarization
Currents by Testosterone
Chang-Xi Bai, MD, PhD; Junko Kurokawa, PhD; Masaji Tamagawa, BE;Haruaki Nakaya, MD, PhD; Tetsushi Furukawa, MD, PhD
Department of Bio-informational Pharmacology , Medical Research Institute, Tokyo Medical and Dental University
Effects of testosterone on APD
Effects of testosterone on membrane currents
Dose-dependence of ICa,L suppression and IKsenhancement by testosterone
Effects of nisoldipine and chromanol 293B on APD shortening
Role of NO in testosterone-induced APD shortening, ICa,L suppression, and IKs enhancement
Effects of inhibitors of the signal molecules in thenongenomic pathway of testosterone
Phosphorylation of Akt and NOS3 bytestosterone
Effects of testosterone on isolated Langendorff-perfused hearts
Proposed schematic model for regulatory mechanismof testosterone on ICa,L and IKs
Role of Nitric Oxide in Ca2 Sensitivity of the SlowlyActivating Delayed Rectifier K Current in
Cardiac Myocytes
Chang-Xi Bai, Iyuki Namekata, Junko Kurokawa, Hikaru Tanaka, Koki Shigenobu, Tetsushi Furukawa
Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University
Effects of A23187 on [Ca2]i, APD, and IKs
Effects of IKs blocker and ICa,L blocker onA23187-induced APD shortening
NO and NOS3 play a role in the A23187-induced APD shortening and IKs enhancement
CaM, but not Akt, is involved in A23187-induced IKs enhancement and APD shortening
Effects of A23187 on interactions of NOS3 with Cav-3 and CaM
Effects of digoxin and rise in [Ca2]o on[Ca2]i, APD, and IKs
The University of Oklahoma Health Sciences Center2005-2009
Activation of TRPP2 through mDia1-dependent voltage gating
Chang-Xi Bai1, Sehyun Kim1, Wei-PingLi1,3, Andrew J Streets2, Albert CM Ong2
and Leonidas Tsiokas1,*
Department of Cell Biology, University of Oklahoma Health SciencesCenter, Oklahoma City, OK, USA
The EMBO Journal (2008) 27, 1345–1356 | & 2008 European Molecular Biology Organization | All Rights Reserved 0261-4189/08
www.embojournal.org
&2008 European Molecular Biology Organization The EMBO Journal VOL 27 | NO 9 | 2008
THE
EMBO
JOURNAL
Colocalization of mDia1 and TRPP2 at the plasma membrane of LLC-PK1 cells
Voltage-dependent block of endogenous TRPP2 by mDia1 in LLC-PK1 cells
Voltage-dependent block of TRPP2 by activated mDia1
Structure–function analysis of mDia1
Voltage-dependent gating of TRPP2 by purified mDia1 in inside-out patches
Activation of TRPP2 by EGF through mDia1-dependent gating
Proposed scheme of the mDia1-dependent regulation of TRPP2
Formation of a new receptor-operated channel by
heteromeric assembly of TRPP2 and TRPC1 subunits
Chang-Xi Bai1*, Aure´lie Giamarchi 2*, Lise Rodat-Despoix 2, Franc¸oise Padilla 2, Tamyra Downs1,
Leonidas Tsiokas1+ & Patrick Delmas 2+
EMBO reports
scientific report
Figure 5. Co-localization of TRPP2 and TRPC1 in primary cilium and association in rat kidney membranes(A) LLC-PK1 cells were stained with a monoclonal antibody to acetylated a-tubulin (611B) (red) or rabbit polyclonal antibody to TRPC1 (green). mIMCD3 cells were stained with a mouse polyclonal antibody to TRPC1 (1F1) (red) or rabbit polyclonal antibody to TRPP2 (green). ( B) Mouse pre-immune serum (lane 2) or mouse polyclonal a-TRPC1
(lane 3) was used to immunoprecipitate endogenous TRPC1. Lane 1: 100 µg kidney membrane lysates were immunoblotted with chick en a-TRPP2.
199
1 2 3
TRPP2
lysate
s (
inp
ut)
Pre
-im
mu
ne
a-T
RP
C1
A B
a-tubulin TRPC1 merge
TRPC1 TRPP2 merge
LL
C-P
K1
mIM
CD
3
128
85