Today

• House Keeping–RT-PCR result–Final report (manuscript style)

• C-value paradox.ppt

• Phylogeny, COI: snail species ID-ed

• fungal sequences

RT-PCT results

snail 1 2 3 6 6 7 8 9 9

LBP/BPI

PhysalysinPGRP

eIF2

group 1 2 3 5 6 7 8 9 10ho

usek

eepi

ngE

xper

imen

tal

PARASITES AND SNAILPARASITES AND SNAIL BIOLOGY BIOLOGY

“identity, possibilities”phylogenetics

“intentions”transcriptomics

PCRrDNA/mito

BioanalyzerDNA-free,

direct sequencing

gel electrophoresisnanodrop spec

Sequence ID (BLAST)editing

Phylogenetics

electrophoresisRT-PCR

gel

CTAB

Trizol

TA cloning, B/W screening

M13 sequencing

Primer design, walking

Qiagen plasmid extraction Restriction digests

DNA

RNA

GenBank submission

+Sevilleta snails

Radix swinhoei?

C-value paradox.ppt

Physid snail species identification

Physella acuta

Based on alignmentof experimentally obtainedpartial COI encoding sequenceswith sequences from popset 164430598For phylogenetic tree construction using parsimony

Majority Rule

Majority Rule Extended

Majority Rule Extended

Majority Rule Extended

Majority Rule